Sulfite DOC316.53.

01137

Colorimetric Method1
0.10 to 5.00 mg/L SO32– Reagent Solution
Scope and application: For boiler water and foodstuffs.
1 Reagent sets for this method are only available in the European Union.

Test preparation

Instrument-specific information
Table 1 shows all of the instruments that have the program for this test. The table also
shows sample cell and orientation requirements for specific instruments.
To use the table, select an instrument, then read across to find the applicable information
for this test.
Table 1 Instrument-specific information
Instrument Sample cell orientation Sample cell
DR 6000 The fill line is to the right. 2495402
DR 3800
DR 2800
DR 2700
DR 1900
DR 5000 The fill line is toward the user.
DR 3900

Before starting
Analyze the samples immediately. The samples cannot be preserved for later analysis.
The temperature of the samples and reagents must be 15–25 °C (59–77 °F) for accurate results.
The sample pH must be 3–10 for accurate results.
Review the Safety Data Sheets (MSDS/SDS) for the chemicals that are used. Use the recommended personal protective
equipment.
Dispose of reacted solutions according to local, state and federal regulations. Refer to the Safety Data Sheets for disposal
information for unused reagents. Refer to the environmental, health and safety staff for your facility and/or local regulatory
agencies for further disposal information.

Items to collect
Description Quantity

Sulfite Reagent A 5 drops

Sulfite Reagent B 2 drops
Pipet, serological, graduated, 10 mL 1
Pipet filler, safety bulb 1
Sample cells (For information about sample cells, adapters or light shields, refer to Instrument-
2
specific information on page 1.)

Refer to Consumables and replacement items on page 3 for order information.

1
Sample collection
• Analyze the samples immediately. The samples cannot be preserved for later
analysis.
• Collect samples in clean glass or plastic bottles.

Test procedure

Start

1. Start program 692 2. Prepare the sample: 3. Add 5 drops of Sulfite 4. Swirl to mix.
Sulfite HPT 430. For Use a pipet to add 10.0 mL Reagent A (HPT 430 A) to
information about sample of sample to a sample cell. the prepared sample.
cells, adapters or light
shields, refer to Instrument-
specific information
on page 1.

5. Add 2 drops of Sulfite 6. Swirl to mix. 7. Start the instrument 8. Prepare the blank: Fill a
Reagent B (HPT 430 B) to timer. A 3-minute reaction second sample cell with
the prepared sample. time starts. 10 mL of sample.
Do not move the sample cell
during the reaction time.

Zero

9. Clean the blank sample 10. Insert the blank into the 11. Push ZERO. The 12. Clean the prepared
cell. cell holder. display shows 0.00 mg/L sample cell.
SO32–.

2 Sulfite, Colorimetric Method (5.00 mg/L)

 Read

13. When the timer expires, 14. Push READ. Results

insert the prepared sample show in mg/L SO32–.
into the cell holder.

Interferences
Interfering substance Interference level
Sulfide More than 5 mg/L

Method performance
The method performance data that follows was derived from laboratory tests that were
measured on a spectrophotometer during ideal test conditions. Users can get different
results under different test conditions.
Program Standard Precision (95% confidence interval) Sensitivity
Concentration change per 0.010 Abs change
692 3.00 mg/L SO32– 2.51–3.49 mg/L SO32– 0.04 mg/L SO32–

Summary of Method
The reagents react with sulfite to form a yellow complex. The measurement wavelength is
435 nm.
Consumables and replacement items
Required reagents

Description Quantity/Test Unit Item no.

Sulfite Colorimetric Reagent Set1, includes: — 100/pkg HPT430

Sulfite Reagent A 5 drops 16 mL —
Sulfite Reagent B 2 drops 5.8 mL —

Required apparatus

Description Quantity/test Unit Item no.

Pipet, serological, graduated, 10 mL 1 each 53238

Pipet filler, safety bulb 1 each 1465100

1 Available only in Europe.

Sulfite, Colorimetric Method (5.00 mg/L) 3

 FOR TECHNICAL ASSISTANCE, PRICE INFORMATION AND ORDERING: HACH COMPANY
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On the Worldwide Web – www.hach.com; E-mail – [email protected] FAX: (970) 669-2932

© Hach Company/Hach Lange GmbH, 2007, 2010, 2012, 2014, 2016. All rights reserved. 11/2016, Edition 9