EXPERIMENTAL CHAPTERS FINAL Edited
EXPERIMENTAL CHAPTERS FINAL Edited
INTRODUCTION
Hand Sanitizer: Aid for Proper Hygiene”, contains several parts which include the
background of the study, objectives of the study, significance of the study, time and place
One of the dirtiest parts of a person’s body is his hands. Every day, people do so
many tasks related to their chores at home and their functions in their workplace and in
school. Because of these, hands catch various forms of germs and bacteria that could
World Health Organization (2017) defines hygiene as the instance and established
way of conduct which aids to uphold and preserve health and further prevent the spread
of diseases. Hand hygiene, on the other hand, is the active process of practically
performing hand washing, antiseptic hand-wash, and alcohol-based hand rub. It is also
described to be the efficient yet short-rubbing of all surfaces of the hands with lathered
soap which is then followed with rinsing and cleansing under flowing streaming water.
Hand washing complies with manually getting rid of visible short-term contaminants
Furthermore, Amoo (2017) emphasized that one of the basic and most effective
ways of preventing the spread of infection is by proper hand hygiene which involves an
1
alcohol-based hand rub. Hence, students need to be encouraged and re-educated about the
In the school setting, as mentioned in the study of Wang (2014), one way to
decrease risk of illness is for learners to engage in good hand hygiene practices prior to
eating. The challenge to hand hygiene is that schools have such busy curricula with
insufficient time for the learners to wash their hands prior to eating. Environmental
obstacles also impede compliance. There are often too few hand sinks available. Even if
students to properly wash their hands. Furthermore, some are too high for easy access and
many are located outside the classroom where supervision is limited. Soap and paper
towels are frequently in short supply and hot water is often not available in many school
bathrooms.
Hand washing with soap and water is always preferably more efficient in
removing and reducing the number of microbes on the hands. It is more effective to
perform both hand hygiene and use antiseptic alcohol-based hand rubs. The use of
alcohol hand sanitizers, however, is mostly implemented when there is no soap and
water.
Rogers (2018) said that hand sanitizer, also called as hand antiseptic or hand rub
is an agent applied to the hands for the purpose of removing common pathogens or
disease-causing organisms. It typically comes in foam, gel, or liquid form. Their use is
recommended when soap and water are not available for hand washing or when repeated
hand washing compromises the natural skin barrier causing scaling or fissures to develop
2
on the skin. Although the effectiveness of hand sanitizer is variable, it is employed as a
simple means of infection control in a wide variety of settings, from day-care centers and
schools to hospitals and health care clinics and from supermarkets to cruise ships.
keeping up with good hygiene can be a tough job to do since it requires effort, time, and
expensiveness of hygiene kit that one actually buys and also of not having enough wash
time because of possessing lots of tasks are actually some of the reasons why people do
not invest time and effort on good hygiene especially so that practicality in their day to
day lives are of their topmost priority. Hence, finding an alternative source for making
sources, can be accessed by many for its immediate availability in the locale. It is a
common term and genus of flowering plants in the rue family, Rutaceae. These plants are
large shrubs or small trees, reaching 5 to 15 m tall, with spiny shoots and alternately
arranged evergreen leaves with an entire margin. They are also notable for their
fragrance. Their juices contain a high quality of citric acid giving them their characteristic
sharp flavor. The citrus fruits’ pure extract is an all-natural body odor eliminator and
antiperspirant. Also, citrus extracts can improve the beauty and quality of skin and hair”
(Coursehero, n.d.).
3
Moreover, citrus hand sanitizers are convenient, portable, easy to use, and not
time consuming. These are free from chemicals and preservatives so that hands will not
seen from the market and in the surroundings. It will effectively clean hands especially
on removing grease and killing bacteria or microorganisms rather than using soap and
water. Effectiveness from hand sanitizers is best when a large volume of product is
Aside from these facts highlighting the benefits of citrus fruits in the form of a
juice, several studies have also been conducted to prove its worth and potential as a hand
sanitizer.
Hence, the researchers are eyeing on the potential of citrus fruit, particularly the
“key lime” or “dayap” (Citrus aurantiifolia) as its locale term in the Ilocos Region, as a
home-made hand sanitizer. “The key lime is a citrus hybrid with a spherical fruit, 2.5cm
to 5 cm in diameter. It is usually picked while it is still green but it becomes yellow when
ripe. It is smaller and seedier, with a higher acidity and a stronger aroma. It is valued for
its unique flavor compared with other limes. It is also known as West Indian
through the use of hand sanitizers supported by relevant studies, the researchers were
prompted to conduct a study to determine the effectiveness of key lime hand sanitizer as
4
Objectives of the Study
1. To determine the effectiveness of the hand sanitizer made from key limes (Citrus
2. To determine whether the key lime (Citrus aurantiifolia) hand sanitizer is more
To the School. This study will help the school promote proper hygiene
among the learners through the use of key lime (Citrus aurantiifolia) hand
importance of proper hygiene and introduce the key lime (Citrus aurantiifolia)
To the Teachers. This study will help the teachers educate their learners
about the importance of proper hygiene through the use of key lime (Citrus
To the Parents. This study will help the parents to further monitor their
children’s proper hygiene and support the products produced by their children
5
To the Learners. This study will help the learners know the importance
of proper hygiene through the use of key lime (Citrus aurantiifolia) hand sanitizer
which will encourage the learners to further produce more alternative products
To the Researchers. This study will help the researchers enhance their
To the Future Researchers. This study will help the future researchers in
The study was conducted at Sta. Lucia Academy, Inc. from June 2019 to February
2020.
Definition of Terms
Key Lime (Citrus aurantiifolia) Hand Sanitizer. This refers to the output or main
product of the study with the key lime or “dayap” as the main ingredient as an aid for
proper hygiene.
Aid for Proper Hygiene. This refers to the ability of key lime (Citrus aurantiifolia) hand
Effectiveness. This refers to the degree of usefulness of the desired output of the key
6
Sta. Lucia Academy, Inc. This refers to the educational institution where the study was
conducted.
School Year 2019 – 2020. This refers to the inclusive academic year when the study was
conducted.
many cultural cuisines, and in juice production. It is widely used because of its
anti-lipedema, and antioxidant properties. Moreover, it can protect the heart, liver, bone,
and prevent urinary diseases. Its secondary metabolites are alkaloids, carotenoids,
coumarins, essential oils, flavonoids, phenolic acids, and triterpenoids. The other
naringenin, nobiletin, and rutin - all of these contribute to its remedial properties”
Alexander (2019) elaborated that citrus fruits trees, like key limes, have come to
gain a worldwide recognition for their suiting refreshing juice, nutritious value and
numerous health benefits and maintenance. Their applied health therapeutic uses have
diseases and macular degeneration. Key lime juice, say for instance, has been shown to
medicines.
7
According to Sales (2019), the calamansi citrus fruit is more effective at killing
microorganisms than soaps. In addition, the lemon lime hand sanitizer which the
Papermates, Inc. in Noteworthy, America (2011) promotes has proven also the efficacy
of citrus fruits in decreasing bacteria on the skin that could cause disease. Nezza Naturals,
their natural hand sanitizer which is made up of lemon lime and tea tree essential oils.
Moreover, the Essential Oils (EO) products originally formulated in America, is making
its way to the Philippines, highly utilize citrus fruits as the main ingredient of their
products such as hand sanitizers, perfumes, and lotions. One of which is their organic
sanitizer made with lemon and orange, all belong to the citrus family.
including salad bars and bowls of whole fresh fruit, opportunities for the transmission of
According to Shulman (2006), one should consider the alcohol content such as
those which are acceptable forms and not the hazardous one. On the other hand, an
alcohol content of less than 60 percent is not enough to be effective. All dirt, blood and
soil must be wiped or washed away first if the alcohol in the sanitizer is to be effective. In
such cases, hand-washing with soap and water is advised. Hand sanitizers are not
8
cleaning agents and are not meant as a replacement for soap and water, but as a
complementary habit. Sanitizers are most effective when used in conjunction with
diligent hand-washing. The benefits of hand sanitizers are convenient, portable, easy to
use and not time- consuming. Several studies have concluded that the risk of spreading
stomach and respiratory infection is decreased among families who use hand sanitizers.
Commercially prepared hand sanitizers contain ingredients that help prevent skin
dryness. Using these products can result in less skin dryness and irritation than hand
washing. The use of hand sanitizers is a habit that one can help keep in all exposed to
fewer germs, and therefore may decrease the chance of illness. Whether one is on the
playground, using someone else's computer or visiting a friend in the hospital, take the
time to rub some on your hands. It is an easy step toward a healthy living.
9
CHAPTER II
MATERIALS AND METHODS
This chapter presents the materials and methods used in the conduct of the study.
Materials
This part presents the materials used in the conduct of the experiment.
The products used which were classified into substances and wares. The
substances include two (2) key limes (Citrus aurantiifolia), one (1) cup of aloe vera gel,
two (2) tablespoons of glycerin, three (3) droplets of sweet orange essential oil, and one-
half (½) cup of water. The wares include tablespoon, measuring cups, cutter or knife,
Methods
This part presents the research design and treatments, experimental procedure,
which aimed to determine the effectiveness of the key lime (Citrus aurantiifolia)
hand sanitizer. The data were gathered through a laboratory experiment using a
hardened nutrient agar, a general purpose nutrient medium used for the cultivation
10
The researchers administered their experiment in gathering data with three
(3) set-ups: one (1) control set-up and two (2) experimental set-ups, and each set-
up has three (3) trials. There are nine (9) petri dishes used in the experiment; each
set-up used three (3) petri dishes for each trial. The petri dishes were filled with
boiled mixture of water and nutrient agar which was cooled until it solidified.
The first set-up was the control set-up (without hand sanitizer) where the
soilidified agar was swabbed with wet cotton buds that were rubbed on
unsanitized hands. The second set-up was the experimental set-up 1 (commercial
hand sanitizer) where the solidified agar was swabbed with wet cotton buds that
were rubbed on sanitized hands that used a commercial hand sanitizer. The third
set-up was the experimental set-up 2 (key lime (Citrus aurantiifolia) hand
sanitizer) where the hardened agar was swabbed with wet cotton buds that were
rubbed on sanitized hands that used key lime hand sanitizer. The process in each
of the set-ups were done three (3) times for each of the three (3) trials but with
different hands. The trials in each set-up were all stocked for four (4) days to let
Experimental Procedure
The following steps were used in the conduct of the experiment:
First, prepare all the needed ingredients and materials in making the key
lime (Citrus aurantiifolia) hand sanitizer. Then, cut the aloe veras into half and
scrape the gel until you earn one (1) cup of it. Put it on a clean basin. Put the
scraped gel in a blender and blend it to remove the lumps. Set it aside on a basin.
11
Add two (2) tablespoons of glycerin on the blended aloe vera gel as moisturizer
to the sanitizer to prevent dry skin. Add one-half ( 12 ) cup of water to lessen the
viscosity of the sanitizer. Grate and squeeze the key lime (Citrus aurantiifolia).
Add the key lime (Citrus aurantiifolia) juice and zests which are the main
ingredients of the sanitizer. Put three (3) drops of essential oil to add an
appealing smell to the sanitizer. Choose the appropriate essential oil that will
blend the smell of the key lime (Citrus aurantiifolia). Mix all the ingredients
together. Stir it carefully to properly mix the ingredients. Put it into a clean
12
Below are the pictures of the actual procedure in conducting the experiment.
Substances Wares
and
13
Data Gathering Instrument
laboratory experiment through a solidified nutrient agar. The solidified agar was
placed into petri dishes. Wet cotton buds were rubbed to different hands which
include the hands which were unsanitary, the hand which was sanitized with the
commercial hand sanitizer, and the hands which were sanitized with key lime
(Citrus aurantiifolia) hand sanitizer. The wet cotton buds were slightly swiped
into the hardened agar in a zigzag line pattern. Each of the hardened agar were
used in three set-ups with three trials: three trials in control set-up (without hand
2 (key lime hand sanitizer). All of them were stocked for four (4) days to let the
bacteria grow. The number of bacteria were gathered through manual counting
Statistical Analysis
solidified nutrient agar, swabbed with wet cotton buds that were rubbed on
14
sanitized and unsanitized hands. The series of trials administered utilized a
commercial hand sanitizer referred to as experimental set-up 1, the key lime hand
sanitizer referred to as experimental set-up 2, and hands that did not used any
hand sanitizer referred to as control set-up. It shows that the average number of
experimental set-up 2 is 24. This means that experimental set-up 2 has the lowest
number of bacteria present, thus it implies that the key lime hand sanitizer is more
15
CHAPTER III
RESULTS AND DISCUSSION
This chapter of the study presents the findings, conclusions, and recommendations
of the study.
Findings
Based from the gathered data, the following findings were revealed:
1. The control set-up (without hand sanitizer) has grown 32 bacteria in its first trial;
the experimental set-up 1 (commercial hand sanitizer) got the highest number of
grown bacteria which has 83 bacteria in its first trial; and the experimental set-up
2 (key lime (Citrus aurantiifolia) hand sanitizer) got the lowest number of grown
2. The control set-up (without hand sanitizer) got the highest number of grown
bacteria which has 81 bacteria in its second trial; the experimental set-up 1
(commercial hand sanitizer) has 44 bacteria in its second trial; and the
experimental set-up 2 (key lime (Citrus aurantiifolia) hand sanitizer) got the
lowest number of grown bacteria which has 8 bacteria in its second trial.
3. The control set-up (without hand sanitizer) got the highest number of grown
bacteria which has 105 bacteria in its third trial; the experimental set-up 1
(commercial hand sanitizer) has 49 bacteria in its third trial; and the experimental
set-up 2 (key lime (Citrus aurantiifolia) hand sanitizer) got the lowest number of
16
4. The control set-up (without hand sanitizer) got the highest average number of
grown bacteria which is 73 within its three trials; the experimental set-up 1
(commercial hand sanitizer) has the average number of 59 bacteria within its three
trials; and the experimental set-up 2 (key lime (Citrus aurantiifolia) hand
sanitizer) got the lowest average number which has 24 bacteria within its three
trials.
Conclusions
Based from the findings of this study, the following conclusions were drawn:
1. On the first trial, it can be gleaned from the findings that the experimental set-up 2
2. On the second trial, it can be observed from the findings that the experimental set-
up 2 (key lime (Citrus aurantiifolia) hand sanitizer) has a lesser grown bacteria
compared to the control set-up (without hand sanitizer) and experimental set-up 1
(commercial hand sanitizer), which means that the key lime is effective as a
sanitizer.
3. On the third trial which recorded the highest number of grown bacteria, it
revealed based on the findings that the experimental set up-2 (key lime (Citrus
hand sanitizer).
17
4. Based on the three trials conducted, the researchers therefore conclude that the
experimental set up-2 (key lime (Citrus aurantiifolia) hand sanitizer) is effective
other hand, without the use of hand sanitizer, there is a greater possibility that
Recommendations
Based from the conclusions of the study, the following recommendations were
formulated:
1. Key Lime (Citrus aurantiifolia) Hand Sanitizer, a cheaper and a safer way of
sanitizing bare hands to remove bacteria since it only contains natural ingredients,
yet effective without alcohol content. Aside from this, the use of key lime (Citrus
microorganisms on hands though it only requires less time than hand washing and
are more accessible than sinks. Added to these benefits is the fact that hand
sanitizers that do not contain alcohol can actually improve the texture of the skin
of hands and can address skin problem conditions such as dry skin, and could as
2. The school administrators and teachers shall reiterate the importance of proper
hygiene to the students of Sta. Lucia Academy, Inc. This shall be sustained as
18
4. The learners should know the importance of proper hygiene using the key lime
(Citrus aurantiifolia) hand sanitizer and produce other alternative products that
5. The future researchers should be conducted to further validate the content of this
study.
19
BIBLIOGRAPHY
-production-technology/exploitative-beneficial-effects-of-citrus-fruits.
handle/10024/128300/NNRNS12K%20Amoo%20Thesis.pdf?
sequence=1&isAllowed=y.
Coursehero. (n.d). Scope and limitations of the study covers how citrus fruits can be.
Limitations-of-the-Study-The-study-covers-how-citrus-fruits-can-be/.
https://www1.health.gov.au/internet/ publications/publishing.nsf/Content/ohp-
enhealth-manual-atsi-cnt-l~ohp-enhealth-manual-atsi-cnt-l-ch3~ohp-enhealth-
manual-atsi-cnt-l-ch3.7.
Meyer, L. (n.d.). Guacalina Nursery & Broker Retrieved from https://www. guacalina
.com /citrus/meyer-lemon.
20
Narang, N. & Jiraungkoorskul, W. (2016). Anticancer activity of key lime, citrus
pubmed /28082795/.
Query, V. (2016). The origin of modern personal hygiene standards. Retrieved from
http://theycallmeoystergirl.com/the-origin-of-modern-personal-hygiene
standards/.
/topic/hand-sanitizer.
Rural Information Hub. (2019). Health promotion and disease intervention: Social
promotion/2/theories-and-models/social-cognitive.
Sajjad, A., & Sajjad, S. S. (2014). Aloe vera: An ancient herb for modern dentistry-a
210463/.
https://www.canadianliving.com/health/prevention-and-recovery/article/hand-
sanitizers-benefits-and-limitations.
scholar. org/6173/aa968a222ecaacbf015af61c75edc6e2b23a.pdf.
21
World Health Organization. (2017). WHO guidelines on hand hygiene in health care: A
handhygiene_summary.pdf.
22
APPENDIX A
STATISTICAL TABLE
23
APPENDIX B
PICTORIALS
24
TRIAL 1
TRIAL 2
TRIAL 3
25
BIOGRAPHICAL SKETCH
of Mr. George P. Soriano (+) and Mrs. Imelda R. Soriano. She currently
Barangobong Sta. Lucia, Ilocos Sur on April 22, 2002. Her religious
Central School, Barangobong, Sta. Lucia, Ilocos Sur. She took her secondary education at
Sta. Lucia Academy, Barangobong, Sta. Lucia, Ilocos Sur. At present, she is currently
enrolled in the Science, Technology, Engineering and Mathematics strand and hopes to
life and to trust herself that she can pass all the struggles that she is going to face while
she is in the process of reaching her goals. Her motto in life is, “Forget the risk and take
the fall. If it’s what you want then it’s all worth it.”
26
BIOGRAPHICAL SKETCH
currently resides at Calongbuyan, Candon City, Ilocos Sur and was born
at Ilocos Sur District Hospital, Sta. Lucia Chapter on July 12, 2002. His
Elementary School, Calongbuyan, Candon City, Ilocos Sur. He took his secondary
education at Sta. Lucia Academy, Barangobong, Sta. Lucia, Ilocos Sur. At present, he is
currently enrolled in the Science, Technology, Engineering and Mathematics strand and
His vision in life is to make his family happy as they see him grow and pursue his
dreams. Also, to see everyone, especially his siblings and friends, as professionals and
have a happy life. He believes in the saying, “Believe you can, and you’re halfway
there.”
27
BIOGRAPHICAL SKETCH
son of Mr. Manuel S. Dela Cruz and Mrs. Evelyn S. Dela Cruz. He
currently resides at Bao-as, Sta. Lucia, Ilocos Sur and was born at
Elementary School, Bao-as, Sta. Lucia, Ilocos Sur. He took his secondary education at
Sta. Lucia Academy, Barangobong, Sta. Lucia, Ilocos Sur. At present, he is currently
enrolled in the Science, Technology, Engineering and Mathematics strand and hopes to
communicate with people from different countries and become aware of social issues. In
other words, an interconnected world. He believes in the saying that, “No man is an
island.”, because everyone has the right to know and learn everything they desire freely.
28
BIOGRAPHICAL SKETCH
the son of Mr. Nikita Osana and Mrs. Desiree Osana. He currently
resides at Bao-as, Sta. Lucia, Ilocos Sur and was born at Bao-as, Sta.
Roman Catholic.
Elementary School, Bao-as, Sta. Lucia, Ilocos Sur. He took his secondary education at
Sta. Lucia Academy, Barangobong, Sta. Lucia, Ilocos Sur. At present, he is currently
enrolled in the Science, Technology, Engineering and Mathematics strand and hopes to
His vision in life is to live a simple but successful life and to create something that
is useful to the future generation. He believes in the saying that, “God first, before
others.”
29
BIOGRAPHICAL SKETCH
currently resides at Banbanaba, Sta. Lucia, Ilocos Sur and was born at
Banbanaba, Sta. lucia, Ilocos Sur on June 15, 2002. Her religious
Memorial School, Alincaoeg, Sta. Lucia, Ilocos Sur. She took her secondary education at
Sta. Lucia Academy, Barangobong, Sta. Lucia, Ilocos Sur. At present, she is currently
enrolled in the Science, Technology, Engineering and Mathematics strand and hopes to
Her vision in life is to live about the truth, to express her love, to stay who she is
no matter what, and to keep fighting for her dreams as a result for a happy life. She
believes in the saying, “No beauty shines brighter than a good heart.”
30
BIOGRAPHICAL SKETCH
She currently resides at Villa Hermosa, Sta. Cruz, Ilocos Sur and was
born at Gov. Roque B. Ablan Sr. Memorial Hospital, Laoag City, Ilocos
Catholic.
She finished her elementary education at Sta. Lucia Catholic School, Burgos, Sta.
Lucia, Ilocos Sur. She took her secondary education at Sta. Lucia Academy,
Barangobong, Sta. Lucia, Ilocos Sur. At present, she is currently enrolled in the Science,
Technology, Engineering and Mathematics strand and hopes to become a Doctor in the
future.
Her vision in life is to tell other people and let them realize that there is always a
good and positive side of life that can lead them to better future. She believes in the
31
BIOGRAPHICAL SKETCH
She is the daughter of Mr. Leon E. Hafalla and Mrs. Ana Elizabeth H.
Hafalla. She currently resides at Vical, Sta. Lucia, Ilocos Sur and was
School, Luba, Sta. Lucia, Ilocos Sur. She took her secondary education at Sta. Lucia
Academy, Barangobong, Sta. Lucia, Ilocos Sur. At present, she is currently enrolled in
the Science, Technology, Engineering and Mathematics strand and hopes to become a
Her vision in life is to become successful so that she can repay all the sacrifices of
her parents just to educate her. She believes in the saying, “Trust the timing of your life.”
32
BIOGRAPHICAL SKETCH
daughter of Mr. Robert Valdez and Mrs. Rowena Valdez. She currently
Calongbuyan, Candon City, Ilocos Sur on May 22, 2002. Her religious
School , Ayudante, Candon City, Ilocos Sur. She took her secondary education at Sta.
Lucia Academy, Barangobong, Sta. Lucia, Ilocos Sur. At present, she is currently
enrolled in the Science, Technology, Engineering and Mathematics strand and hopes to
Her vision in life is to be the person who speaks for one that cannot, to listen for
the one that cannot hear, and see for the one without sight. She believes in the saying,
“No one ever injured their eyesight by looking on the bright side.”
33