Q bank

Biochemistry
Every Chapter
Every Important Question
Marks distribution

Akshat Juneja med_pie

 General
Biochemistry
Long Answer Questions
1. Classify carbohydrates with examples

2. What are the functions of carbohydrates.

3. Describe mucopolysaccharides.
4. Classification of lipids.
5. Describe phospholipids.
6. Describe saturated & unsaturated fatty acids
7. Discuss amphipathic lipid
8. Classification of amino acids
9. Discuss organization of protein structure
10.Classification of proteins
11.Describe the structureof DNA.
12.Different types of RNAs
13.Classification and nomenclature of enzymes
14.Various factors affecting enzyme activity.
15.Describe the Mechanism of enzyme action.
16.What are coenzymes? Role of coenzymes in
enzyme action.
17.Importance of serum enzymes in the diagnosisof
disease
18.What are coenzymes?

Akshat Juneja med_pie

 General
Biochemistry
Long Answer Questions

1. Classify vitamins & discuss their functions &

deficiency disorders.
2. Describe the chemistry,biochemical
functions,daily requirements, sources &
deficiency manifestations of vitamin A.
3. Discuss Folic Acid Involvement in Iron
Metabolism.
4. Describe the chemistry,biochemical
functions,daily requirements, sources &
deficiency manifestations of vitamin C.
5. Describe the chemistry,biochemical
functions,daily requirements, sources &
deficiency manifestations of vitamin D.
6. Describe the biochemical functions of vitamin B1
7. Discuss Vitamin B Complex Coenzymes

Akshat Juneja med_pie

 General
Biochemistry
3,1 Mark, MCQ Topics Questions

Epimers Enzyme specificity

Mutarotation Competitive inhibition
Sugar derivatives Coenzymes

Anomers Allosteric enzymes

Enediol lsoenzymes

Amino sugars K value

Phosphatidylinositol Lactate dehydrogenase

Sphingomyelins Role of metals in enzyme

action
Essential fatty acids
Active site
Cis-trans isomerism
Vitamin D is a hormone-
Zwitter ion
justify
Denaturation
Thiamine pyrophosphate
Chargaff’s rule
Coenzymes of niacin
Pernicious anemia
Folate trap
Tocopherol
Vitamin K in carboxylation
Biotin

Akshat Juneja med_pie

 Plasma
Proteins
Long Answer Questions

1. Describe the characteristics & functions of plasma

proteins.
2. Give an account of different types of
immunoglobulins & their functions.
3. Describe the structure of different
immunogloublins.
4. Discuss acute phase proteins

3,1 Mark, MCQ Topics Questions

Electrophoresis of plasma proteins

Functions of albumin
Antitrypsin
Haptoglobin
Multiple myeloma
Bencejones proteins
Anticoagulants

Akshat Juneja med_pie

 Haemoglobin
Long Answer Questions

1. Describe the structure of hemoglobin and

oxygen transport.
2. Write an account of hemoglobinopathies & sickle-
celal nemia.
3. Discuss the biosynthesis of heme.
4. What are porphyrias? Describe any 3 porphyrias
in detail.
5. Explain degradationof heme
6. Discuss jaundice.

3,1 Mark, MCQ Topics Questions

Methemoglobin
Bohr effect
Sickle cell anemia and malaria
Thalassemias
Carboxyhemoglobin

Akshat Juneja med_pie

 Biological
Oxidation
Long Answer Questions

What are the high-energy compounds in metabolism

Describe the components of ETC
Define oxidative phosphorylation
Discuss chemiosmotic hypothesis in detail.
Explain the enzymes involved in biological oxidation.
Discuss about the inhibitors of ETC and oxidative
phosphorylation

3,1 Mark, MCQ Topics Questions

ATP synthase
Redox potential
Cytochromes
Sites of oxidative phosphorylation
ATP as energy currency.

Akshat Juneja med_pie

 Metabolism
Long Answer Questions
1. Describe the metabolism of glucose 6-phosphate.
2. Give an account of TCA Cycle.
3. Give an account of glycogen metabolism.
4. How citric acid cycle is the final common
metabolic pathway for the oxidation of
foodstuffs.
5. Discuss the synthesis of glucose from non-
carbohydrate sources.
6. Describe the hexosemonophosphate shunt and
its significance
7. Give an account of cholesterol biosynthesis
8. Add a note on the plasma lipid profile.
9. Describe the extramitochondrial synthesis of
fattyacids.
10.Write types,characteristics & metabolism of
lipoproteins.
11.Give an account of fatty acid oxidation
12.Describe Synthesis of urea.
13.Give an account of the formationof specialized
products from glycine.
14.Discuss the metabolism of phenylalanine and
tyrosine.
15.Describe the fate of carbon skeleton of amino
acids.

Akshat Juneja med_pie

 Metabolism
Long Answer Questions

1. Describe the catabolism of purine nucleotides

and the associated metabolic disorders.
2. Write an account of the biosynthesis of
inosinemonophosphate
3. Discuss the synthesis and degradation of
pyrimidines
4. Describe the role of PRPP in purine and
pyrimidine synthesis.
5. Write an account of salvage pathway in purine
nucleotide synthesis

Akshat Juneja med_pie

 Metabolism
3,1 Mark, MCQ Topics Questions

Glycogenolysis
Galactosemia
Cori cycle
2, 3- BPG
Glycogen storage diseases
Essential Fructosuria Energetics of TCA cycle
TPP in carbohydrate metabolism
Carnitine
LCAT
Fattyliver
Ketonebodies
Lipotropic factors
Acyl carrier protein
Degradation of cholesterol
HDL One-carbon metabolism
Amino acid pool Albinism
Transmethylation Serotonin
Transamination Glutamate and glutamine
Deamination Polyamines
Ammonia toxicity

Akshat Juneja med_pie

 Metabolism
3,1 Mark, MCQ Topics Questions

Gout
Functions of nucleotides
Oroticaciduria
Degradation of uric acid in different animals
Regulation of purine synthesis
Inhibitors of purine synthesis.

Akshat Juneja med_pie

 Minerals
Long Answer Questions

1. Write on the trace elements and their

metabolism in the body.
2. Discuss the biochemical functions,dietary
requirement, sources and absorption of
calcium.
3. Describe Iron Metabolism.
4. Describe the metabolismof copper,zinc and
manganese.
5. Write the biochemical importance and disease
states of fluorine and selenium.

Akshat Juneja med_pie

 Minerals
3,1 Mark, MCQ Topics Questions

Homeostasis of calcium
Osteoporosis
Phosphorus
Sodium and chlorine
Factors affecting Fe absorption
Hemosiderosis
Wilson’s disease

Akshat Juneja med_pie

 Organ Function
Tests
Long Answer Questions

1. Write the different laboratory investigations

employed to assess liver function tests.
2. Discuss the biochemical parameters for the
differential diagnosis of jaundice.
3. Describe the renal function tests.
4. Discuss the different laboratory investigations
to evaluate gastric functions?

Akshat Juneja med_pie

 Organ Function
Tests
3,1 Mark, MCQ Topics Questions

Vanden Bergh reaction

Prothrombinimeas
Renal threshold substances
Creatinine clearance
Standard urea clearance
Urine concentration test

Akshat Juneja med_pie

 Nutrition
Long Answer Questions

1. Define BMR. Discuss the factors affecting BMR.

2. Define balanced diet. Formulate a diet for a
labourer.
3. Discuss protein-energy malnutrition &
differentiate Marasmus & Kwashiorkar.
4. Give an account of the RDA for macro-and
micronutrient

Akshat Juneja med_pie

 Nutrition
3,1 Mark, MCQ Topics Questions

Caloric value of foods

Specific dynamic action
Energy requirements of man
Kwashiorkor
Nitrogen balnce
Biological value of protein

Akshat Juneja med_pie

DNA Replication
Long Answer Questions

1. Describe the replication of DNA.

2. Describe recombination of DNA.
3. Discuss different types of DNA damages and
the repair mechanisms.
4. What are mutations? Describe different types
of mutations
5. Give an account of telomeres and their role in
senescence and cancer

Akshat Juneja med_pie

DNA Replication
3,1 Mark, MCQ Topics Questions

Replication fork
Inhibitors of DNA replication
Telomerase
Holliday model of DNA recombination
Transposition
Mismatch repair
Xeroderma pigmentosa

Akshat Juneja med_pie

 Transcription
Translation
Long Answer Questions

1. Give an account of transcription.Compare the

RNA synthesis between prokaryotes and
eukaryotes.
2. Describe translation
3. Discuss the inhibitors of transcription and
translation.
4. Explain post-transcriptional and post-
translational modifications.
5. What is genetic code? Describe the
characteristics of genetic code.

Akshat Juneja med_pie

 Transcription
Translation
3,1 Mark, MCQ Topics Questions

hnRNA
Eukaryotic RNA polymerases
Introns and exons
Reverse transcription
Wobble hypothesis
Anticodon
Shine-Dalgarno sequence
Chaperones

Akshat Juneja med_pie

 Remaining
Genetics
Long Answer Questions

1. Describe lactose(lac) operon.

2. Describe the principles of recombinant DNA
technology.
3. Explain nucleic acid blotting techniques
4. Describe the polymerasechain reaction along
with its applications.
5. Write on the utility of DNA in disease diagnosis
& forensics.

Akshat Juneja med_pie

 Remaining
Genetics
3,1 Mark, MCQ Topics Questions

One cistron-one subunit concept

DNA methylation
Gene amplification
Restriction endonucleases
Plasmids
Methods of gene transfer
Western blotting
Gene libraries
RFLPs
Recombinant Vaccine

Akshat Juneja med_pie

 Advanced
Biochemistry
Long Answer Questions
1. Explain Mechanisms of detoxification
2. Enumerate Sources & Generation of Free
Radicals.
3. Enumerate Antioxidants in Biological System
4. Describe structure, biosynthesis, regulation,
mechanism of action of Insulin.
5. Write on Regulation of Blood Glucose Levels.
6. Describe Diabetes Mellitus & it’s classification
7. Describe Glucose Tolerance Test
8. Describe Molecular Basis of Cancer
9. Explain Virus Structure & Immunological
Abnormalities in HIV
10.Explain Course & Lab Diagnosis of HIV
11.Explain Types & Applications of
Chromatography
12.Explain Electophoresis & Photometry

Akshat Juneja med_pie

 Advanced
Biochemistry
3,1 Mark, MCQ Topics Questions

Cytochrome P450
Glycosylated Hb
Oncogenes
Tumor Markers
Vaccine against AIDS? Comment !
Beer Lambert Law

Akshat Juneja med_pie