Introduction To Soil Science Laboratory Manual (Schwyter and Vaughan)
Introduction To Soil Science Laboratory Manual (Schwyter and Vaughan)
SOIL SCIENCE
LABORATORY MANUAL
(SCHWYTER AND
VAUGHAN)
Intro to Soil Science Laboratory Manual by Anna R. Schwyter & Karen L. Vaughan is licensed under a Creative Commons
Attribution-NonCommercial-ShareAlike 4.0 International License.
Introduction to Soil Science Laboratory Manual
Anna R. Schwyter
Karen L. Vaughan
2020
(unable to fetch text document from uri [status: 403 (Forbidden)])
TABLE OF CONTENTS
This text explores the fundamental principles of soil science and soils as a natural resource. Students will be introduced to the physics,
chemistry, microbiology, morphology, fertility and management of soils, and to the processes driving soil formation. The major soil
types of the world and their classification will be discussed, and the relation of major soil characteristics to soil productivity,
conservation and sustainability will be addressed.
TITLE PAGE
1: INTRODUCTION TO SOIL SCIENCE AND SOIL FORMATION
GOAL: To better understand the concepts of soil formation and applications of methods for describing and identifying soils
1.1: INTRODUCTION
1.2: ACTIVITY 1 - SOIL FORMATION
1.3: ACTIVITY 2 - SOIL HORIZONS
1.4: ACTIVITY 3 - SOIL COLOR
1.5: ACTIVITY 4 - SOIL TEXTURE
1.6: ACTIVITY 5 - SOIL STRUCTURE
2.1: INTRODUCTION
2.2: ACTIVITY 2 - MINERALS
2.3: ACTIVITY 2 - ROCKS
2.4: ACTIVITY 3 - WEATHERING
2.5: ACTIVITY 4- ROCKS TO PARENT MATERIAL
3: SOIL TAXONOMY
GOAL: To understand the main soil factors that contribute to the classification of a soil and how to use the system of Soil Taxonomy.
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5.9: ACTIVITY 1 - BULK DENSITY AND POROSITY OF SOIL CORES
5.10: ACTIVITY 2 - SOIL MOISTURE
5.11: ACTIVITY 3 - SOIL WATER MOVEMENT
5.12: ACTIVITY 4 - INFILTRATION ESTIMATES AND AVAILABLE WATER CALCULATIONS
5.13: ACTIVITY 5 - EFFECTS OF SOIL EROSION
6: SCIENCE COMMUNICATION
GOAL: To learn how to effectively communicate scientific ideas and understandings to a variety of audiences – with a focus on soil
science!
6.1: INTRODUCTION
6.2: ACTIVITY
7: SOIL CHEMISTRY
GOAL: To understand key aspects of chemical nature, properties, and reactions in the soil-environment.
7.1: INTRODUCTION
7.2: ACTIVITY 1 - SOIL COLLOIDS
7.3: ACTIVITY 2 - SOIL CHARGE
7.4: ACTIVITY 3 - SOIL CHARGE CALCULATIONS
7.5: ACTIVITY 4 - SOIL PH AND ELECTRICAL CONDUCTIVITY
7.6: ACTIVITY 5 - REDUCTION AND OXIDATION REACTIONS
9: FIELD LAB
9.1: OVERVIEW
9.2: CLIMATE
9.3: RELIEF
9.4: TIME
9.5: SOIL CLASSIFICATION
9.6: SOIL ORDERS
9.7: SLOPE AND RUNOFF
9.8: SOIL PERMEABILITY
9.9: LARAMIE'S LOCAL SOILS
9.10: ACTIVITY 1 - SOIL FORMATION
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10.12: ACTIVITY 5 - WINOGRADSKY COLUMNS
BACK MATTER
INDEX
GLOSSARY
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CHAPTER OVERVIEW
1: INTRODUCTION TO SOIL SCIENCE AND SOIL FORMATION
GOAL: To better understand the concepts of soil formation and applications of methods for describing and identifying soils
1.1: INTRODUCTION
1.2: ACTIVITY 1 - SOIL FORMATION
1.3: ACTIVITY 2 - SOIL HORIZONS
1.4: ACTIVITY 3 - SOIL COLOR
1.5: ACTIVITY 4 - SOIL TEXTURE
1.6: ACTIVITY 5 - SOIL STRUCTURE
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1.1: Introduction
☰
Soil is the medium for plant growth at the land surface. Most of the world's food supply comes from plants growing in the
soil (oceans and lakes supply some food). Soil serves as the habitat for thousands of species of animals, insects, and
microorganisms. Soils support buildings, highways and other structures. Soils capture rainwater and melting snow to
recharge our precious ground water. Soils also adsorb air and water pollutants. Soil microorganisms constitute the major
component of life in the earth and cause many major biochemical transformations essential to our lives.
Throughout the duration of this course you wil dive deeper into the concepts and understandings of each of the following
sub-disciplines, or focal areas of interest, within the discipline of soil science:
By nature of these sub-disciplines, soil science is inherently an interdisciplinary field. For this first lab, we will begin to
learn a bit more about this interdisciplinary science by understanding the fundamentals of soil formation, soil
characteristics, and the methods used for identifying and describing soils.
SOIL FORMATION
Soil is defined as: “a natural body comprised of solids (minerals and organic matter), liquid, and gases that occurs on the
land surface, occupies space, and is characterized by one or both of the fol owing: horizons, or layers, that are
distinguishable from the initial material as a result of additions, losses, transfers, and transformations of energy and matter
or the ability to support rooted plants in a natural environment” (Keys to Soil Taxonomy, 2012).
Soils exist in three dimensions (pedon; 3 dimensional bodies) containing inorganic and organic material generally
arranged into layers from the land surface downward into the earth. These layers of topsoil and subsoil (being roughly
parallel to the ground surface) are called horizons. A two-dimensional vertical section of soil extending through these
horizons downward to the parent material is called a profile.
Soils form in place based on five natural soil forming factors, including: 1) Climate; 2) Organisms; 3) Relief; 4) Parent
Materials; And, 5) Time (Figure 1). Collectively, these five factors contribute to the function of soil formation (S), S= f
(Cl, O, R, P, T…), otherwise known as pedogenesis. As the diagram suggests, soil formation is a continuum. As any one
factor or influence changes, the final soil product will vary. An infinite number of combinations of soil forming processes
yield many different soil properties.
Understanding the role of each soil forming factor allows us to understand or predict soil occurrence.
These five soil forming factors also work through four important soil forming processes (Table 2), considered in the
following four groups: 1) Additions; 2) Losses; 3) Transformations; And, 4) Translocations.
Figure 1. “The soil forming factor continuum” by Colby J. Moorberg and David A. Crouse is licensed under CC by 4.0
Table 1. Examples of soil forming factors
Soil Forming Factor Example
While these factors and processes contribute to the natural formation of soil, it is also acknowledged that soil formation is
likewise impacted by what is referred to as, “the human factor”. As such, we all play a part in the formation, conservation,
and protection of soil. As we manipulate the earth's surface, we can either improve or degrade our quality of lives.
Regardless of the effect, we become responsible for the stewardship of our land – and good land stewardship requires us to
understand the properties and processes of soils.
Soil Horizons
The horizon is different from the layers above and below it in the same soil. In total, there are five major soil horizons,
called master soil horizons, which are distinguishable by their color, composition, and other characteristics.
O horizon
An O horizon consists of organic material deposited on top of the mineral surface. Often, various stages of decomposition
can be recognized, with fresh material at the surface and decayed material below.
Most soils do not have an O horizon or, at best, they have only a thin O horizon either because soil microorganisms rapidly
decompose the organic material, or very little organic material is deposited on the soil surface due to a lack of vegetative
growth. However, some forest soils may have an appreciable accumulation of organic matter as an O horizon. This may
consist of broadleaf litter or more commonly as a thick layer of pine needles.
Soil color
Color is one of the most important physical characteristics of soil. It can be used to differentiate among various soil
horizons (layers of soil), to estimate organic matter content, to evaluate the soil drainage class, and to assess many other
soil conditions. The Munsell Color System, as approved by the National Bureau of Standards, is used to identify soil color.
The Munsell Color System has been developed internationally to describe any color anywhere. It has nothing to do,
directly, with soil.
In the Munsell Color System, three component parts describe the colors: hue, value, and chroma (always designated in
exactly this order). Hue is the spectral variable. Hue represents one of the dominant colors of the rainbow, for example,
yellow or red. Value represents the relative darkness or lightness of the color. Chroma represents the purity, strength, or
saturation of a color. Colors having zero chroma are gray to black.
A Munsell Color Book is used to identify soil color. The book's pages display chips of various colors, which are matched
as closely as possible to the soil color in the field. Each page represents a different Hue. Common pages used for soil
studies include 10R, 5YR, 10YR, and 2.5Y hues. The letter R represents Red and the letter Y represents Yellow. Red soils
are common when iron oxides are abundant in the soil profile. The pages go from 2.5R (pink) and 5R (yel owish pink),
both of which are seldom used, to 7.5R (reddish orange) to 10R (dark reddish orange; commonly associated with iron
oxides). The hues, which consist of increasing amounts of yellow mixed with red, are designated as 2.5YR (brownish
orange), 5YR (strong brown), 7.5YR (moderate brown), and 10YR (medium brown). As the red is eliminated, more yellow
is emphasized with 2.5Y (light olive brown) and 5Y (moderate olive). For a few strongly flooded soils, which have no free
oxygen gas, the colors are very dark blue, dark greenish, dull bluish gray, or black.
Soil Texture
The solid portion of soils consists primarily of mineral particles (usually more than 95 % by mass) mixed with organic
materials. These mineral particles are divided into coarse fragments greater than 2 mm in diameter (termed gravel), and
finer particles less than 2 mm in diameter.
The fine particle fraction is further divided into arbitrary size classifications known as soil separates. The three soil
separates are sand, silt, and clay. These three separates remain essentially constant during your lifetime in any given soil.
The size classification of these soil separates is shown in Table 3.
Table 3. Size Classification of Soil Separates
Particles Diameter (mm)
Soil texture is defined by the relative proportion of sand, silt, and clay. Soil texture remains constant for any given soil
during your lifetime. Because texture is limited to sand, silt and clay, other soil components, including water, air, organic
matter, and inorganic materials larger than 2 mm, are excluded, although gravels and cobbles may be included as modifiers
to the textural name. Texture applies only to mineral soils. Organic soils such as peat and muck, do not have texture.
Percentage is determined by multiplying the decimal fraction of two numbers by 100 %. The symbol % means “per cent”
which can be translated mathematical y to mean “per” = to divide by the number, and “cent” = 100. Thus % means “divide
by 100" which is the same as to multiply by (1/100). Thus, to multiply by 100 % is the same as multiplying by 100 x
(1/100) which equals 1.0. Therefore, multiplying any decimal fraction by 100% does not change the value of the original
decimal fraction because it is the same as multiplying by 1.0. Note, it is incorrect to multiply only by 100. The correct
form is to multiply by 100%, not by 100 alone.
The percentages of sand, silt and clay are calculated from laboratory data according to the following definitions:
mass (g) of sand
%Sand = × 100%
mass (g) of sand + silt + clay
17 g silt
%Silt = × 100 = 34.0% = 34.0 pph silt in soil
50.0 g dry soil
17.5 g clay
%C lay = × 100 = 35.0% = 35.0 pph clay in soil
50.0 g dry soil
Soils having similar textures can be grouped into classes of soils having similar characteristics and behavior. Twelve soil
textural classes are given in the textural triangle (Fig.2). The textural classes provide a way to express soil texture without
referring specifically to the percentages of each separate.
When any two of the three percentages are known, the soil textural class can be determined from the triangle. This is why
only two axes are required to plot the textural class for any given soil.
Figure 2. USDA Soil Survey Division Staff, SoilTextureTriangle, marked as public domain, more details on Wikimedia
Commons
Locate the percent sand along the bottom (horizontal axis) of the triangle. Locate the percentage of clay along the left-hand
axis of the triangle. Move upward from the sand percentage and to the right of the clay percentage until these two
projected lines intersect. This point of intersection corresponds to the textural class corresponding to this particular portion
designated on the triangle.
If a soil falls on the boarder of a texture (in-between textures), soil scientists have adopted the convention of adding 1%
clay to the actual value and reporting the textural class name associated with this 1% clay addition. The reason for making
this decision is based upon the fact the clay content is the most difficult to analyze of the three soil separates.
A soil's textural class may be determined by several methods that range from quick and simple field tests to more involved
laboratory techniques. The "field" or “feel" method is the simplest to conduct, but it is the least accurate due to differences
among individuals. The feel method is qualitative more than quantitative. The feel method involves moistening the soil,
kneading the moistened soil mass with your fingers to mix the particles thoroughly, and feeling the particles to determine
the texture. The accuracy of estimating soil texture by this method improves with experience. Examples of the soil textures
using the feel method are provided in descriptive form.
Determining the Soil Textural Class by the "Feel/Field" Method
The soil textural class can be estimated by observing and feeling the soil under dry, moist, and wet conditions. The size
range of the separates and their feel when moist are listed in Table 4.
Table 4. USDA soil particle size ranges and feel under moist conditions.
Soil Structure
Soil structure is the arrangement of the individual soil particles into aggregates which are separated by surfaces of
weakness. The structural aggregates, which are called peds, comprise several types depending on their shape: granular,
blocky, prismatic, columnar, and platy. The definition has two parts: aggregation and separations. If a soil's condition does
not satisfy both parts of the definition, it is structureless. Thus, not all soils have structure. The two structureless conditions
are single grained (having no aggregation) and massive (having no separation patterns).
Granular soil structure… occurs most commonly in the A horizon. Granular structure forms as a result of the soil humus
attracting strongly to the surface of the silt and clay particles of the soil. This attraction multiplied over many years creates
aggregates. The binding between the humus of one aggregate and the next is less than the binding of the humus to the silt
and clay particles. Consequently, the aggregates act independently. The humus causes the individual aggregates to arrange
much as marbles would in a random arrangement with many voids or pore spaces between the aggregates. These pores
allow for rapid water permeability. These same pores permit rapid air movement and allows for easy root penetration.
Granular structure is optimized in the presence of the very fine roots of grasses. Granular structure can form within 50 to
100 years of exposing new parent material.
Blocky soil structure… is most commonly found in the B horizon. Blocky structure occurs as a result of the long, slow
process of clay particles moving from the A horizon (eluviation) and leaching into the B horizon (illuviation). As these
clay particles accumulate in the B horizon, they settle inside existing pores. Consequently, the soil pores become lined with
these il uviated clay particles. With time, more of these pores become fil ed with clay, thus squeezing the pores tighter
together and reducing water permeability, air movement and ease of root penetration. These individual aggregates take on
a block-like appearance.
Prismatic soil structure… usually forms after blocky structure has been formed in the B horizon of a soil. Prismatic
structure is usually associated with soils which have formed in semiarid and arid environments where the soil dries out to a
considerable depth during some period of the year. This pul s the blocks apart and eventually leads to a whole group of
blocks acting as a vertical prism. These prisms form zones of weakness between the prisms vertically. During the first rains
of the wet season, water rapidly moves down along these prisms, but as the soil becomes wetter, the prisms expand, fil ing
the voids between the prisms resulting in moderate water permeability. This causes restricted air movement and reduces
the ease of root penetration through this horizon.
Columnar soil structure… occurs in the B horizon of some soils in semiarid and arid regions. Columnar structure only
occurs where the soil has a high concentration of sodium on the soil cation exchange sites. The columns form in a similar
manner to the formation of the soil prisms. However, as the soil continues to form, the sodium disperses the clays from the
surface of the prism and causes these clays to move down the edges of the prisms. This causes a strong degree of sealing of
the prisms resulting in slow to very slow water permeability, restricted air movement, and very difficult root penetration.
Few plants can grow in such soil.
Platy soil structure… is relatively rare in native soils. Platy structure is most commonly formed as a result of compaction
by vehicles, livestock, and humans. Platy structure is most commonly found in the A horizon of agricultural, urban, forest,
and range soils under intensive management. The key factor is relatively horizontal layers of these soil aggregates.
Single grain soil structure… occurs where no grain is connected to any other grain. The best example is dune sand. Sands
and silts in an alluvial (river deposit) or loess (wind deposit) may have a single grain structure. Single grain structure is a
lack of structure since no true aggregates exist.
Massive soil structure… is really the lack of structure. As the term massive suggests, this is simply a mass of soil particles
with no apparent repeating shape across the horizon. Massive structure usually occurs in the C horizon but may occur in
other horizons if very little soil horizon development has occurred.
3) Please fill in the following blanks and answer the questions referring to each of the soil forming factors indicated
below:
Soil forming factor Contributing element
1. _______________________
Climate 2. Precipitation
3. Evaporation
1. Human influence
Organisms 2. ________________________
3. ________________________
1. ________________________
Relief
2. Erosion/sedimentation on the landscape
Give two examples of a parent material type:
Parent Material 1. _________________________
2. _________________________
Why would it be important to assess the
Time
formation of soil over time?
4) Indicate whether each of the following examples represents an ADDITION, LOSS, TRANSFORMATION, or
TRANSLOCATION within the soil. Note: some answers are used more than once. (6 points)
a. Clay particles are moved by water moving from an upper E horizon to a lower B horizon within a soil profile…
b. The forces of nature have acted upon the soil over time, weathering sand sized particles into smaller clay sized
particles within the top horizon of a soil profile…
c. Surface mineral soil material is washed away by the force of a monsoon…
d. Organic material is mixed into the upper 20cm of the soil profile by the activity of burrowing worms…
e. Dust particles fall on to the surface of a soil…
f. Nitrate is leached from the A horizon within the soil profile…
A
B
C
6) Moisten the soil and determine its Munsell Color in this new moisture condition. Record results on the appropriate line
below.
Moist Soil Munsel Color Notation Color Name
A
B
C
USDA-NRCS, Texture by Feel, marked as public domain, more details on Wikimedia Commons
1
2
3
1
2
Soil Sample Sand % by mass Silt Clay USDA Soil Textural Class
A 65 25 10 Sandy Loam
B 40 40 20
C 50 25 25
D 10 70 20
E 40 10 50
F 17 46 37
5) What is the lowest clay percentage allowed for a texture to be classed as:
6) What is the lowest silt percentage allowed for a texture to be classed as:
7) A dry soil sample weighing _25_ grams in total contains _8_ grams of sand, _15_ grams of clay, and the remainder is
silt. Calculate the percentages of sand, silt and clay and determine the soil's textural class. Show the appropriate units and
complete setup for each calculation of this problem.
% Sand =
2.1: INTRODUCTION
2.2: ACTIVITY 2 - MINERALS
2.3: ACTIVITY 2 - ROCKS
2.4: ACTIVITY 3 - WEATHERING
2.5: ACTIVITY 4- ROCKS TO PARENT MATERIAL
1 11/8/2021
2.1: Introduction
Soils don’t simply exist on the landscape; rather they grow, develop, erode, and slowly transform into other soils through
time—a group of processes known collectively as pedogenesis. Pedogenesis begins with parent material, the stuff from
which soils form. Although a small minority of soils develop in organic deposits, most derived from inorganic parent
materials acted upon by additions, losses, translocations, and transformations
A major type of transformation is weathering of geologic parent materials. All mineral soils develop from the rocks and
minerals of the earth's crust. Through weathering, minerals and rocks physically disintegrate into smaller particle sizes,
and chemicaly decompose into altered chemical and mineralogical products. However, the original rock material
commonly is transported or reworked by some mechanism before a soil is formed. Variations in the history of the initial
material lead to a range of types of soil parent materials (Table 1). Additional y, these weathering processes release
elements, including plant and animal nutrients, and convert primary minerals into clays.
Table 1. “Summary of transported parent materials” by Colby J. Moorberg and David A. Crouse is licensed under CC by 4.0
Mode of transportation Mode of deposition Name of parent material
Stream Alluvium
Water Lake Lacustrine
Ocean Marine
Ice Till, moraine
Ice
Meltwater Outwash (alluvium, lacustrine)
Wind Wind Loess, dunes
These parent materials continue to weather over time based on a function of soil forming factors and processes. Another
major consideration for weathering potential of soil parent materials is hillslope position. Hillslope position references
where on the landscape a soil is located (Figure 1). Hillslope positions essential y delegate the degree of slope and
interaction with the groundwater table for the soils of a specific position location. These relationships between soil and
slope, or the groundwater table, greatly influence weathering processes over time, increasing both physical and chemical
weathering, depending on hil slope position.
Figure 1. "Hill slope position across a landscape" Positions higher up and with a higher slope wil have better drainage and
less interaction with the groundwater table, while those positions that are lower lying on the landscape have a lower slope
and decreased drainage. Wysocki et al., 2000 and Schoeneberger et al., 2012.
MINERALS
In geology and soil science, a mineral is a naturally occurring, inorganic, crystalline solid having a definite chemical
composition and predictable physical properties. Of the six criteria included in this definition, three (naturally occurring,
inorganic, and solid) need little elaboration; the remaining three are explained in succeeding paragraphs.
Crystalinity of Minerals
Crystalline means that a mineral’s atoms are arranged in an orderly and repeatable manner; they are not random. The
atomic arrangement, which varies among mineral groups, contributes to several of the mineral’s physical properties,
Element Atomic Symbol Plant Available Form Element Atomic Symbol Plant Available Form
In addition to the 18 elements required by plants, animals also require arsenic (As), chromium (Cr), fluorine (F), iodine (I),
lithium (Li), sodium (Na), selenium (Se), silicon (Si), and tin (Sn). Herbivorous animals obtain these elements by eating
plants, which take up the elements, even though the plant might not need them. Carnivorous animals, in turn, obtain the
elements by preying on other animals.
Regardless of whether the elements are required by plants or animals, all except carbon, hydrogen, oxygen, and nitrogen,
are made available initially by weathering of minerals. The elements gradually become concentrated in the biosphere
through biogeochemical cycles, including the carbon and nitrogen cycles, which will be covered in a later laboratory.
ROCKS
The most common rocks are consolidated assemblages of minerals, but a few (e.g., obsidian, pumice, and coal) comprise
non-crystalline (hence, nonmineral) materials. Despite the great number of possible compositions and arrangements, rocks
can be divided into three categories based on their mode of formation: igneous, sedimentary, and metamorphic.
Igneous Rocks
Intrusive and Extrusive: Igneous rocks form when molten material called magma cools and solidifies.
Although the magma originates tens of kilometers below Earth’s surface, it can solidify at any depth from its depth of
origin to the surface. Magma that remains at depth while cooling and solidifying forms igneous intrusive rocks. Because of
extremely slow cooling, minerals can grow to macroscopic size, and can be readily identified in a hand specimen.
Instead of remaining deep throughout the cooling process, some magmas erupt to the surface, where they cool and solidify
rapidly. The resulting igneous extrusive rocks can be glassy (i.e., amorphous) or microcrystalline, although many contain a
smattering of macrocrystals that formed before eruption. In erupting, the magma might ooze toward the surface as lava, or
it might explode violently into the atmosphere, forming pumice and volcanic ash.
Felsic and Mafic: In addition to having distinctively different crystal sizes, igneous rocks also vary in mineralogical
composition because of chemical differences among magmas. Some rocks are dominated by quartz and orthoclase
(potassium-rich feldspar) because they derived from magma that was rich in silica and potassium. These rocks, which tend
to be light colored, are called felsic. Other igneous rocks have little or no quartz or orthoclase, but instead are characterized
by calcic plagioclase (calcium-rich feldspar) and ferromagnesian minerals (e.g., augite and hornblende). These are called
mafic and are dark colored. Other rocks of intermediate compositions can be found between these extremes.
Table 6. An example of elements combining to form minerals and minerals combining to form an igneous rock.
Sedimentary Rocks
Sedimentary rocks, which make up the bulk of Earth’s continental crust, can be either clastic (i.e., fragmental) or
nonclastic (i.e., nonfragmental). See Table 7 for examples.
Clastic rock: consist of fragments of previously existing rocks (igneous, sedimentary, or metamorphic) that have been
transported to a new location (often an ocean bottom), where they were deposited and buried layer upon layer, and
cemented together. The deposits can be several thousand meters thick!
The fragments, or clasts, can vary in size to include clay, silt, sand, gravel, and cobbles.
Nonclastic rock: form by chemical precipitation of dissolved salts and by biochemical precipitation of organically derived
compounds in water, most commonly on the floors of shallow seas. Organisms that generate nonclastic rocks include
bacteria, algae, diatoms, corals, and mollusks. The most common nonclastic sedimentary rocks are limestone and dolomite
(also called dolomitic limestone). Others include rock gypsum, chert, and coal.
Although the two major categories are recognized, most sedimentary rocks are a combination of clastic and nonclastic,
nonetheless they usually are dominantly one or the other. For example, the sand grains in sandstone are clastic, but the
cement that holds them together can be nonclastic.
Table 7. Classification of sedimentary rocks.
Clastic Nonclastic
Metamorphic Rocks
Metamorphic rocks form primarily by the application of extremely high heat and pressure to some previously existing
rock. The original rock may be igneous, sedimentary, or even metamorphic, but sedimentary rocks are most susceptible to
metamorphosis. Metamorphic rocks can be either foliated or nonfoliated. Foliated rocks consist of minerals that are
aligned or oriented to yield a somewhat stratified or layered appearance, whereas the minerals in nonfoliated rocks are
about equally arranged in all directions, giving them a more uniformly massive appearance (Table 8).
Table 8. Classification of metamorphic rocks.
Foliated
Metamorphic Rock Common Original Rock Appearance/Comments
WEATHERING
Weathering breaks rocks and minerals down, resulting in the production of soils. Weathering occurs via two processes:
physical disintegration (breaking into smaller pieces) and chemical decomposition (changing the nature of the minerals
themselves).
Physical disintegration causes rock masses to split apart or to abrade and wear away from the larger rock surface.
Freezing and thawing, abrasion by particles suspended in wind or water, and grinding caused by glaciers result in
disintegration. Repeated actions of these processes cause stresses, which eventually break and erode rock surfaces.
Chemical decomposition
Chemical decomposition progresses most rapidly when more surface area is available for chemical reactions to occur.
Warm and wet environments enhance chemical decomposition. The smaller the particles, the greater the surface area of a
given volume of rock (the greater the total exposed corners and edges) and the greater the rate of chemical decomposition.
The total number of corners exposed to weathering processes is the major factor in the determination of weathering rates.
This is because a corner has three surfaces and three edges for chemical attack. Chemical weathering is a combination of
five major processes: carbonation, hydration, hydrolysis, oxidation-reduction (redox), and solution.
Hydrolysis and particle size: Hydrolysis is a chemical decomposition process involving the splitting of water molecules
during a reaction. A hydrolysis reaction can be easily identified by looking for water on the left side of a chemical equation
and the absence of water (but the formation of H+ and/or OH-) on the right side of the equation. All minerals weather by
hydrolysis. The hydrolysis reaction is particularly evident with silicate minerals (e. g. feldspars and micas). Hydrolysis is
the primary reaction resulting in the release of most plant nutrient elements from minerals. The mineral orthoclase will
weather by hydrolysis.
+ −
KAlSi3 O8 + H2 O → HAlSi3 O8 + K + OH
C O2 + H2 O ↔ H2 C O3
Soluble calcium + Soluble hydroxide + Carbonic acid → Low soluble calcite + Water
The second reaction is:
C aC O3 + H2 C O3 → C a(H C O3 )2
Reduced (gray soil colors) ↔ Oxidized (red and yellow soil colors)
Read through and complete the folowing activity and questions. All questions should be answered and completed labs are
due at the end of the laboratory period. No late work wil be accepted.
pyrite
calcite
gypsum
hornblende
muscovite
plagioclase
orthoclase
quartz
granite
basalt
sandstone
schist
limestone
chert
conglomerate
marble
gneiss
shale
Part 2
Your instructor has set out pictures detailing redoximorphic features displaying Oxidation and Reduction reactions.
Observe each photo and detail your observations in the section below.
1. Observe the picture on display for gleyed soils (Image 1). Describe the appearance of the gleyed soil.
2. Observe the colors of the mottled soil on display (Image 2). Describe the appearance of the mottled soil.
3. What are the physical (i.e. color) and chemical (i.e. form of Fe) properties of a gleyed/reduced soil?
4. What environmental condition causes soils to become gleyed?
5. In the field, what soil topographic or slope position (i.e. summit, backslope, or toeslope) would be most
conducive to the formation of reducing conditions or gleyed soils? Why?
6. Most soils do NOT have gleyed horizons. If a gleyed horizon exists, which soil horizon is most commonly
gleyed/reduced in a soil (Assume this soil has an impermeable layer at 100 cm)?
7. Explain why this horizon (from Q #6) would be most commonly reduced or gleyed.
8. Soil may have a mottled condition associated with a fluctuating water table. What are the physical and chemical
properties of a mottled soil? What happens when the water table rises and falls in the mottled soil zone?
9. Well oxidized soils are associated with what soil drainage condition? (See page 1-11 in the Red Book)
In the field, what soil topographic or slope position (i.e. summit, backslope, or toeslope) would be most
conducive to the formation of strongly oxidizing conditions in a soil? Why?
10. For most soils, which is likely to have the most oxides, and most likely to have an oxidized horizon? Why?
Granite
Sandstone
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3.1: Introduction to Soil Taxonomy
☰
The word, “taxonomy” is based on the Greek words “taxis”, meaning arrangement; and “nomia”, meaning method. In
biology, taxonomy refers to a hierarchical system in which organisms are grouped based on shared characteristics, with
domains and kingdoms at the top of the hierarchy, and genus and species at the lowest levels. Similarly, Soil Taxonomy is
a hierarchical system used to group soils based on observable or measurable characteristics. A common application of soil
classification (the act of identifying the taxonomic classification for a given soil) is to develop models of how soils of
different classifications associate with one another within a landscape, which can eventually be used in soil mapping. The
primary concepts of soil classification using Soil Taxonomy will be reviewed in this lab, followed by an overview of the
Web Soil Survey (United States Department of Agriculture Natural Resources Conservation Service, 2016).
Table 1. “Explanation of the Ap horizon description” by Colby J. Moorberg and David A. Crouse is licensed under CC by 4.0
Morphological property Description Morphological property Description
Figure 1. “Organization of Soil Taxonomy with examples” by Colby J. Moorberg and David A. Crouse is licensed under
CC by 4.0
Order
There are 12 soil orders in total (Table 4). The geographical range of each order is dependent upon several environmental
processes and climatic factors (i.e. temperature, precipitation, etc.) leading to soil formation. Soil order is the first to be
determined when classifying a soil.
Table 4. “Simplified key to the 12 soil orders” by Colby J. Moorberg and David A. Crouse is licensed under CC by 4.0 The
bolded syllable in each order is the formative element used in the names of suborders and lower taxonomic levels.
Suborder
Soil suborders are determined based on soil moisture regimes and diagnostic features. Formative elements specify unique
soil properties at each taxonomic level and has a connotation for a given soil.
The formative elements for suborder are listed in, (Table 5).
Table 5. “Formative elements used to identify various suborders in Soil Taxonomy” by Colby J. Moorberg and David A.
Crouse is licensed under CC by 4.0
Great group
The great group expresses the degree of diagnostic horizon expression within each suborder taxa. The formative elements
for great group are listed in (Table 6).
Table 6. “Formative elements for names of great groups and their connotations” by Colby J. Moorberg and David A.
Crouse is licensed under CC by 4.0
Subgroup
A soil’s subgroup is defined by properties that are transitional or similar to those found in other orders, suborders, and
great groups.
Family
The family is typically comprised of more than one component that is determined for the soil in question using information
detailed in chapter 17 of the Keys to Soil taxonomy. The technical family name is comprised of descriptive terms for family
information such as, particle size class and mineralogy of control section, soil temperature regime, CEC, depth, rupture-
resistance, etc.
Figure 2. “Formative elements in the taxonomic classification of the Harney series” by Colby J. Moorberg and David A.
Crouse is licensed under CC by 4.0
To break things down, each soil taxonomical category is described in general detail below, outlining what information
contributes to each of the categories:
Description of defining/diagnostic
Taxonomical category Example
information
*The 12 Soil Orders: (Alfisol, Andisol, Aridisol, Entisol, Gelisol, Histosol, Inceptisol, Mollisol, Oxisol, Spodosol, Ultisol,
Vertisol)
Now, let’s relate similar taxonomical concepts to information and details regarding your hometown, or favorite
place…
ORDER:
Order is the broadest of the categories, while still providing a sense of overall identity. Please select one of the following
states or territories that is most applicable to the location of your hometown/favorite place. If located outside the US,
please note it as, “OUS” (outside U.S.). Your classification text should be as follows: ______, ______, ______, ______
____ ____ HI
California - CA Ohio - OH
Colorado - CO Oklahoma - OK
Connecticut - CT Oregon - OR
Delaware - DE Pennsylvania - PA
Idaho - ID Tennessee - TN
Illinois - IL Texas - TX
Indiana - IN Utah - UT
Iowa - IA Vermont - VT
Kansas - KS Virginia - VA
Kentucky - KY Washington - WA
Maine - ME Wisconsin - WI
Maryland - MD Wyoming - WY
Mississippi - MS Guam - GU
Nebraska - NE Palau - PW
New Jersey - NJ
Suborder:
This describes one of the most defining characteristics of your hometown, whether that be proximity to the coast, overall
moisture conditions, elevation, etc. For example, : If I live in Hawaii, my most defining feature is that I live on an Island,
so I will designate this as…
______, ______, ______, ______ ___ islhi
isl= on an island, hi= in Hawaii
Please select one of the following below that best describes your location:
Anthr- located in a city with a lot of human activity and impact Hi- located at an elevation higher than 5K ft
Coas- located on or near the coast (~15 mi) Isl- located on an island
Cry- cold most of the year Lo- located at an elevation lower than 1K ft
Great group:
This describes your location further in depth, almost like an adjective to describe the prominent feature of your locale. For
example: If I live in Hawaii, I will first say I live on an Island, and on this Island I live extremely close to the coast (<15
mi), so I will designate this as…
______, ______, ______, ______ Coasislhi
Coas = on the coast, isl= on an island, hi= in Hawaii
Please select one of the following great group descriptions below to further detail your location:
Anthr- located in a city with a lot of human activity and impact Lo- located at an elevation lower than 1K ft
Coas- located on or near the coast (~15 mi) Sno- impacted by a lot of snow
Dry- dry land conditions Riv- located near a prominent river or freshwater body
Hi- located at an elevation higher than 5K ft War- warm/hot most of the year
Inl- located inland Woo- wooded area with a high presence of vegetation
Subgroup:
This category will detail the most defining and unique element of your location, that sets it apart from the rest of town.
Consider any of the following below, however, we encourage you to come up with your most defining feature that comes
to mind when you think of the landscape of your location. For example: If I live in Lousiana, I will first say I live in a
warm/hot location, located near the coast, and in a wetland- so, I will designate this as…
______, ______, ______, __Wetty Coaswarla
Please select one of the following subgroup descriptions below to further detail your location:
Anthro- located in a city with a lot of human activity and impact Mounty- live on a mountain
Deserty- desert conditions Rivery- located near a prominent river or freshwater body
Dryish- dry land conditions Roady- live near a major highway or road
Empty- vast lands Typic- very typical location for the state it is located in
Highly- located at an elevation higher than 5K ft Warmish- warm/hot most of the year
Inner- located inland Woodsy- wooded area with a high presence of vegetation
And when we compare our classified location to a similarly classified soil, it looks something like this:
Moisture regimes:
Aquic: (or Perudic): Saturated with water long enough to cause oxygen depletion
Udic: Humid or subhumid climate
Ustic: Semiarid climate
Aridic (or Torric): Arid climate
Xeric: Mediterranean climate (cool, moist winters and warm, dry summers)
2) To illustrate the structure of Soil Taxonomy, separate a complete taxonomic name into the 6 categories. Follow the
example of the Harney silt loam earlier in this laboratory.
a) Colby:
a. Taxonomic Name: Fine-silty, mixed, superactive, calcareous, mesic Aridic Ustorthents
b. Order-
c. Suborder-
d. Great Group-
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4.1: Introduction- Soil Survey Reports
☰
The properties of each soil must be understood to ensure proper use and management. To consider the soil in land use
planning and development, an inventory is essential in determining the location and extent of the various kinds of soil.
This inventory is called a soil survey.
Soil surveys began in 1898 in the United States. The study of soils was initially conducted for agriculture and forestry,
with little attention given to the ways in which soil properties might influence urban use of land. Modern soil surveys now
include information about multipurpose uses of soils. These include interpretation information for wildlife management,
development of parks and recreation areas, construction potentials for super highways, airports, and building foundations,
selection of pipeline right-of-ways, evaluation of the pollution potential from septic tanks, selection of desirable spatial
distribution patterns for residential, commercial, industrial, agricultural, and recreational land use development,
implementation of zoning and subdivision control, and land evaluation for equitable tax assessments and bank loan
appraisals.
Shallow 20-50
Moderately deep or Moderately Shallow 50-100
Deep 100-150
Very deep >150
While still under the Soil Data Explorer tab, select the tab heading entitled Suitabilities and Limitations for Use – it’s located
immediately to the left of the Soil Properties and Qualities tab heading.
9. Determine the suitability of your soil map unit for Dwellings with Basements. This can be found under the Building Site
Development tab (default settings are OK).
What is the rating? _______________
List the reason(s) for the rating _________________
10. Determine the suitability of your soil map unit for Local Roads and Streets. This is also found under the Building Site
Development tab (default settings are OK).
What is the rating? _______________
List the reason(s) for the rating _________________
11. Click on the Vegetative Productivity tab. Determine the suitability of your soil map unit for one of the categories listed (this
will vary depending on geographical location).
List the category (e.g. Yields of Irrigated Crops, Range Production, etc.)
What is the rating? _______________ (describe as needed)
12. Click on the Waste Management tab. Determine the suitability of your soil map unit for Disposal of Wastewater by
Irrigation (default settings are OK).
What is the rating? _______________
List the reason(s) for the rating _________________
GENERAL QUESTIONS
13. In your own words, give a definition of a soil map unit. A couple of sentences should be adequate to do this, but make sure
your definition mentions soil series.
14. Explain why it is necessary to use soil series rather than higher levels of the classification system (i.e. order, suborder) as
the basis for detailed soil mapping.
15. Based on all of the information you have found in Web Soil Survey, provide a general assessment of the selected soil map
units from your AOI relative to their use and management. Describe any relevant soil or site properties that result in
limitations for the variety of land uses that are assessed in Web Soil Survey.
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5.1: Introduction- Soil Texture, Structure and Water Relations
☰
In this lab, you will observe examples of soil textures and structures independently. Second, you will observe the effects of
texture and structure on water percolation. Third, you will measure the water holding capacity of a coarse and fine textured
soil. During the exercise, try to think about how the concepts you are learning can be applied to your major area of interest.
Figure 1. Illustration of the difference between bulk density and particle density by Plant and Soil Science eLibrary used
with written consent.
Some typical values for the bulk density of soils having different textures along with the corresponding porosity
percentages are given In Table 1.
Table 1. Typical bulk density, porosity, and pore size distribution of several soils.
For a particular soil, the bulk density may vary due to compaction or loosening as a result of tillage operations, or other
forms of soil disturbance. Variation of bulk density means the porosity will also change. Some soils (surface of forest soils,
Histosols, and soils derived from volcanic ash) have very low bulk density values (often less than 1.0 gram/cm3), due to
high porosity.
Where Dp is particle density; ODwt is oven-dry weight of soil; Vs is volume of solids. Particle density takes into account
the mass and volume occupied by the solid particles only. It excludes the volume occupied by air and water. Since a
majority of soils is composed of particles derived from minerals containing 70% or more silica and oxygen, the particle
density of most soils is approximately 2.65 g/cm3 . This particle density is nearly the same as the density of quartz.
Variations in the particle density of soils are due to the presence of heavier minerals (such as iron oxides) or organic matter
(which has a low density and reduces the overall particle density value). Kaolinite and illite clays have particle densities of
about 2.6 g/cm3 , while colloidal montmorillonite clays have particle densities of about 2.4 g/cm3.
Although tillage results in a change in both bulk density and porosity, it does not affect particle density. The particle
density remains constant because tillage and other short-term changes do not alter the total amount or the chemical
composition of the soil mineral particles.
Where %P is percent porosity; Vp is volume of pores; Vt is volume total (solids plus pores). The total volume of pores is
usually greater in a well-structured fine-textured (clayey) soil than in a coarse-textured (sandy) soil. However, the
individual pores tend to be larger in sandy soils than in clayey soils (which have many more micro pores). But why can a
soil which has larger pores have less total pore space than a soil which has smaller pores?
Although a soil’s total porosity is important, its pore size distribution is equally important. Individual pores can be
categorized as macro pores and micro pores. The large (macro) pores drain quickly of excess water and allow free
movement of air and water. These macro-pores promote soil aeration (free movement of gases) and enhance water
infiltration, percolation and drainage. Soils containing a large proportion of macro pores are usually very sandy and tend to
retain only a limited amount of water. The small (micro) pores retain much more water, and consequently drain slowly and
have restricted air and water movement. Restricted soil aeration causes reduced plant growth because roots need oxygen to
conduct the process of root respiration in every cell. Also, many microorganisms require oxygen - therefore, some
biological and chemical reactions are inhibited by poor aeration.
Aggregation, or the clustering of the soil particles into aggregates, creates larger macro pores between the peds, which
enhance aeration and root penetration. Within each aggregate exist smaller micro pores that function primarily to retain
water. A balance between macropores and micropores is desirable for most agricultural situations to provide both adequate
aeration and optimum water retention for crop growth.
If the bulk density and the particle density of a soil are known, then the porosity can be calculated by using the following
relationship:
Since, 100 % total soil volume = % solid volume + % pore volume then, % total pore volume = 100% total soil volume -
% solid volume
%P = %Vt − %Vs
This last equation can be used to determine the porosity, if one recognizes the % solid volume can be obtained by dividing
the bulk density by the particle density and multiplying the quantity by 100. The resulting equation becomes:
Db
%P = 100% − ( × 100)
Dp
Note, the units of bulk density and particle density must be the same for them to be able to cancel in the calculation. The
term in brackets, i.e. [bulk density / particle density x 100] represents the percentage of the soil volume occupied by the
soil solids. When the volume of soil solids is subtracted from 100%, the difference is the percentage of the soil volume
occupied by pores, or the total porosity or total pore space.
Figure 2. Uhland sampler for collecting soil samples for bulk density determinations Source: King et al. 2003.
The driving weight is raised and dropped repeatedly to drive the sampler into the soil. When the desired depth is reached,
the device is removed from the soil, and the removable metal cylinder containing the soil sample is removed. With the
dimensions of the cylinder and the weight of oven-dry soil inside the cylinder, we can calculate the bulk density.
Please watch the following video for proper bulk density soil sampling technique; the "core method" is what you will be
using, which begins at 22:20.
Video: A video job aid on the three preferred methods for collecting bulk density samples in soils using National
Cooperative Soil Survey methods and references. https://youtu.be/E7BSZrJ-TDw?t=1341
Figure 3. Detailed description of the association between crop and soil response regions and soilmoisture availability.
Source: Rhoads and Yonts, 1984.
Water moves through a soil in the large pores and drains away due to the force of gravity. This water is called gravitational
water. Gravitational water represents the difference in soil moisture content between the saturation condition and field
capacity.
Gravitational water = soil moisture content at saturation - soil moisture content at field capacity.
Water held within small soil pores by adhesion and cohesion is termed capillary water. Only a portion of the total
capillary water in the soil is available to plants. Plant available water can be obtained from the soil. Plant available water
is the difference between the water content of a soil at field capacity and the soil water content at the permanent wilting
point.
Plant available water = water content at field capacity - water content at permanent wilting point
Available water-holding capacity (plant available water) is an estimate of the water held between field capacity and
permanent wilting point within the rooting zone or the top 150 cm of the soil if there is no root-limiting layer. The total
water is calculated by summing the amount of water held in each horizon or portion of horizon, if the horizon extends
beyond 150 cm. If a horizon or layer is unfavorable for roots, this and all horizons below should be excluded in calculating
the available moisture.1
For most field crops, the permanent wilting point is equal to -1500 kPa of water potential or 1500 kPa of water suction.
When a soil is air dry, the water present is held at water potentials ranging from -3000 to –10,000 kPa. The water in air dry
soil is in equilibrium with the soil pore atmosphere which has about 98% relative humidity. The Hygroscopic Coefficient is
the condition when the last micro pore is drained of water and only films of water exist surrounding the soil particles. Soil
dried at 105°C to a constant weight is considered oven dry. The oven dry weight of soil is used as the reference weight to
quantify the amount of water in mineral soils for all moisture conditions. Hygroscopic water is the water held between the
Hygroscopic Coefficient and Oven dry.
Hygroscopic water content = Water held below the Hygroscopic Coefficient - Oven dry mass
1 For available water calculations, the properties of the lowest horizon designated for description can be assumed to extend
to 150 cm, if the presence of a restrictive layer is not evident. If a restrictive layer is present between the lowest described
horizon and the 150 cm depth, the depth to the restrictive layer should be considered for available water estimations.
Materials not suited for plant root growth include: (i) horizons with coarse sand textures and some unfilled voids located
directly underneath a horizon of finer-textured soil materials (i.e., textures of very fine sand, loamy very fine sand or finer),
(ii) bedrock, (iii) fragipans, (iv) densic materials, (v), horizons cemented across 90% or more of the soil profile, and (vi)
SiC, C, or SC textures that are very firm or firmer and have structureless grade and massive shape of structure.
Worldwide there are approximately five billion hectares of degraded land, which is approximately 43% of the Earth’s land
surface. Of those five billion hectares, the most common cause of degraded land (two billion hectares) is soil degradation
due to erosion, compaction, surface crusting, acidification, or salt accumulation. Most of that degradation (85%) comes
from water and wind erosion, however, other human activities such as overgrazing, industrial activities, unsustainable
agricultural activities, overexploitation, and vegetation removal cause many negative side effects on the land ultimately
causing degradation of the soil (Figure 4).
Figure 4. The negative side-effects (physical, chemical, and biological) caused by various human impacts that degrade soil.
Source: Palm et al., 2007.
Soil erosion includes two separate processes – soil erosion by water, and by wind.
Water erosion… begins with detachment as rain drops bombard soil aggregates, separating some of them from the
aggregate. These stand-alone soil particles are much smaller and are more easily transported. The transported particles are
eventually deposited in a low-lying area, completing the three-part process of detachment-transport-deposition (Figure 5).
Transport can happen due to splashes from the raindrop, or from running water carrying sediment downhill.
Water erosion is initiated by sheet erosion where splashed soil is moved uniformly, but some columns of soil that were
protected by pebbles may remain. When the water gathers into small channels due to irregularities in the landscape, those
channels incise into the soil surface forming a rill. Rills can be smoothed by tillage equipment. If enough water gathers, a
gully can form, which is essentially a large rill that is so deep that it cannot be smoothed by tillage equipment. Interrill
erosion is sheet erosion that occurs between rills. The majority of soil erosion is due to sheet and rill erosion.
1
2
3
Core volume (cm3)
Step 2: Now that you have practiced calculating bulk density and porosity, you will do the same calculations for your
prepared soil samples collected in the first week of lab.
Show your detailed Bulk density and porosity calculations:
Bulk Density = ___________ g/cm3
Porosity = ___________ %
Bulk Density Questions
1. Explain how the addition of organic matter to these soils would affect their bulk density.
2. Which of these soils will usually have the higher bulk density, the sandy soil or clayey soil? Why?
3. How does your bulk density value from your ring sample compare with those given in Table 1?
4. Calculate the bulk density of a soil with a porosity of ___30%___ by using the porosity formula and assuming the
particle density of this soil is 2.65 g/cm3.
1. Out of the following textures below, please indicate which was SOIL A, B, or C.
Sand =
Silty Clay Loam =
Clay =
2. Which texture class did the water move through the fastest? The slowest?
3. Why do you think this occurred?
4. What is a practical application of this method? (Aka why would you want to know this specific information about the
soil?)
Horizon Structure
1
2
3
4
Infiltration estimate
Step 2: Determine the texture for each soil horizon within the range soil profile. Based on your results and the relationship
of texture and infiltration (which you just learned in Activity 3), what do you estimate the available-water holding capacity
will be, (circle one of the following options on the following page):
Horizon Texture
1
2
3
4
1 0
2 5
3 15
4 20
Using the information in the table above, calculate your available-water to a depth of 150 cm for this range soil and using
your final answer determine the final available-water class of this same soil:
Available-water calculated for soil profile = ________________________
Available-water class = ________________________
Follow-up questions:
1. Do your estimated infiltration rates and calculated available-water holding capacity make sense together? Please
explain the relationship between the two, and why or why not they make sense together.
2. Did your estimated available-water holding capacity match your calculated available-water holding capacity? If not,
where could you have assumed or calculated incorrectly?
3. Based on your final calculation would plants grow well in this soil? Why or why not?
6.1: INTRODUCTION
6.2: ACTIVITY
1 11/8/2021
6.1: Introduction
☰
INTRODUCTION
Science communication is a growing area of practice and research and withholds particular value for natural sciences.
Science communication is recognized as the public communication of science-related topics. The act of communicating
science is carried out in many ways, encompassing science journalism, blogging, social media, videos, art, academic
publishing, and any other activity that aims to help people understand anything related to research findings or the scientific
process. Science communication is primarily broken down into two facets: 1) The science of science communication; and
2) The practice of science communication. The science of science communication breaks down what works effectively for
communicating science, how it works, and why it works. While the practice of science communication is the actual
implementation of communicating scientific ideas to a variety of audiences – and often multiple audiences!
7.1: INTRODUCTION
Soil chemistry is primarily concerned with the chemical reactions associated with the many phases incorporated within the soil
mixture. Soil chemistry is an ever-expanding field that traditionally focused on chemical reactions that affected plant growth and
nutrition, and has since expanded to include water and soil contaminants and their effects on plants, animals, and humans.
1 11/8/2021
7.1: Introduction
☰
Soil Chemistry is the branch of soil science that deals with the chemical composition, chemical properties, and chemical
reactions in soil. The soil environment is dynamic and comprised of a heterogeneous mixture of air, water, and inorganic
and organic solids. Soil chemistry is primarily concerned with the chemical reactions associated with the many phases
incorporated within the soil mixture. Soil chemistry is an ever-expanding field that traditionally focused on chemical
reactions that affected plant growth and nutrition, and has since expanded to include water and soil contaminants and their
effects on plants, animals, and humans.
Soil is made up of inorganic and organic assemblages, in the form of solids, liquids, and gases. Elemental content varies
among soil types, however, the elements found in the highest quantities are typically O, Si, Al, Fe, C, Ca, K, Na, and Mg
(in order of highest to lowest natural abundance). Inorganic components of soil makeup approximately 90% of all solid
components, however, soil organic matter (SOM) still plays a critical role in chemical reactions within the soil. In addition,
properties such as size, surface area, and charge behavior affect the majority of the essential equilibrium and kinetic
reactions and processes which occur in soils (Figure 1).
In this lab we will cover important chemical reactions in soil, the properties that affect chemical reactions in the soil, and
how to measure specific chemical properties in soils.
SOIL COLLOIDS
The extremely small, colloidal particles (smaller than 0.002 mm) of clay and humus control many important chemical and
physical properties of the soil. This portion of the soil is often called the "active fraction", comprised of highly reactive
materials with electrically charged surfaces. The small size of colloids results in a large surface area per unit weight, and
their ionic structure results in a net electrical charge.
The type, amount, and mineralogy of colloids will strongly influence most land management decisions. For example a soil
that is 40% of clay that primarily consists of smectite (a 2:1 shrink-swell clay) could have limitations for constructing
roads, or building foundations due to the shifting of the soil as the soil wets and dries. Such a soil could be highly
productive for row crop agriculture though, due to the high amount of charge that facilitates the retention of nutrients like
Ca2+, K+, Mg2+, etc. On the contrary, a soil such as an Oxisol that has 80% clay has colloids that are primarily aluminum
and iron oxides, which do not shrink or swell, and have a low amount of charge. Thus, the soil would be well suited for
building foundations.
Figure 1. "The basic molecular and structural components of silicate clays" (a) (top) Si tetrahedral structure versus,
(bottom) Al, Mn octahedral structure, and b) orientation of tetrahedral and octahedral sheets forming layered crystalline
structure. Adapted from “The Nature and Properties of Soils” Brady and Weil, 2008.
Noncrystalline silicate clays: These clays also consist of tightly bonded Si-Al-O atoms, oriented into unordered,
noncrystalline sheets. They are characterized by high water holding capacity and extremely high capacities to strongly
absorb phosphate and other anions, particularly under acid conditions. Examples of noncrystalline silicate clays include,
allophane and imogolite, typically found in Andisols, or soils derived from volcanic ash.
Iron and aluminum oxides: these properties are present in many soils but are highly expressed in highly weathered soils of
warm, humid regions. They consist of either Fe or Al atoms combined with O atoms, with a net charge that ranges from
slightly negative to moderately positive. Examples of iron oxides include crystalline goethite and soil coatings. Examples
of aluminum oxides include crystalline gibbsite and soil coatings.
Organic material/humus: Organic colloids or humus, are especially important in the upper portions of a soil profile.
Because these colloids are not mineral material, alternatively, they consist of complex chains and rings of carbon atoms
bonded to H, O, and N. These colloids are often the smallest in size, have a net negative charge that varies with soil pH,
and have a high capacity to absorb water.
SOIL CHARGE
Now that we have prefaced the basics of soil colloids, it is important to highlight some of the important processes which
contribute to the potential charge of a soil. To begin, there are two kinds of charge within a soil: 1) Permanent charge and
2) Variable charge.
1. Permanent charge: this charge is determined from isomorphic substitution which takes place in the clay fraction of the
soil between phyllosilicate layers of crystalline silicate clays.
2. Variable charge: this charge is predominantly pH dependent and results from reaction of OH- associated with the
following
Edges of clay minerals
Organic material
Figure 2. "Permanent charge due to isomorphic substitution" Example of isomorphic substitution whereby Mg replaces the
position of Al, still maintaining crystalline structure.
Figure 3. Charge calculations determined for the substitution of one Mg in place of an Al ion. Adapted from “The Nature
and Properties of Soils” Brady and Weil, 2008.
Figure 5. “Schematic of process for determining cation exchange capacity” by Colby J. Moorberg and David A. Crouse is
licensed under CC by 4.0
Calculating CEC
The Sum-of-Cations Method: If you have a soil analysis where the quantities of all cations in the soil are listed, simply
summing all those exchangeable quantities will yield the CEC you found in the preceding problems.
The "Mineralogy" Method: As you know from your reading and class discussion, clay minerals have a range of values for
CEC. If the mineralogy of the clay fraction is known (that is, the type and amounts of each clay mineral), then the CEC
can be approximated.
To make these calculations easier, Table 1 contains representative values for CEC to use in all calculations for this class
unless otherwise noted. In nature, however, these soil colloids will have a range of values.
Table 1. “"Typical CEC of various soil colloids"” by Colby J. Moorberg and David A. Crouse is licensed under CC by 4.0
Mineral or colloid type CEC of pure colloid (cmolc/kg)
kaolinite 10
illite 30
montmorillonite/smectite 100
vermiculite 150
humus 200
Example: Using the mineralogy approach to CEC calculations, consider a soil having 100% clay where the clay is 100%
kaolinite. The CEC would then be 10 cmolc/kg. If a soil contains only 10% kaolinite (or 10 kg clay in 100 kg soil),
however, this clay would contribute:
10 cmolc 10 kg clay 1.0 cmolc
Total CEC of the soil = × =
kg clay 100 kg soil kg soil
Figure 6. Mineral soil pH ranges for crops by Keith Johnson, used with written consent.
Figure 8. The redox ladder and associated redox potential (Eh) thresholds by Vaughan & Duball, 2019.
Redox Diagrams
Redox conditions in soil are represented on a Redox diagram, which displays Eh versus pH (Figure 10). By determining
the pH and the Eh and finding the point of intersection on the Redox diagram one can obtain a fairly good idea about the
general nature of the soil system.
Figure 9. Redox diagram demonstrating the function of pH and redox potential on the fate of reduction for various
chemical compounds. The upper right of a redox diagram represents highly oxidized conditions, while the lower left
represents highly reduced conditions, by Vaughan & Duball, 2019.
The sulfide that forms is very reactive. It forms extremely insoluble metal sulfides (iron-monosulfides (FeS) and pyrites
(FeS2)). These metal sulfides give the solution a black or dark gray appearance. Often an organic mat forms on the surface
of these soils.
Please read through and complete the following activity and questions. All questions should be answered and completed
labs are due at the end of the laboratory period. No late work will be accepted.
2) Describe the major difference between a 1:1 silicate clay and a 2:1 silicate clay.
d. Calculate the CEC from the data in the table using the sum of cations method.
1) Measuring pH:
a. Add 5 g of each soil type into to sterile cup.
b. Add 20 mL of DI water
c. Mix the soil and water until it is a uniform slurry
d. Use pH meter and place electrode into the slurry
e. Wait until the reading stabilize
f. Record pH
g. Rinse and wipe down meter
h. Repeat process for all samples
pH Data-
Sample ID pH
2) Measuring EC:
(Follow procedure for measuring pH)
EC Data-
Sample ID EC
pH and EC questions:
1. Which soil sample had the highest pH? Lowest?
2. What range of salinity does each soil sample meet?
3. If your soil had a pH of 5 what treatment needs to be done so that alfalfa can be planted?
4. What is the pH range for All Textures for Slightly Saline?
Control:
Organic Matter Addition
pH Measurements for →
K2SO4 Addition:
OM+K2SO4:
In addition, your instructor has set out another suite of mesocosm experiments with indicator of reduction in soil (IRIS)
tubes inserted into the soil for one month. Protocol for interpreting IRIS tubes is also on display for you to review. After
interpreting the IRIS tubes please answer the following questions below:
1. Which mesocosms displayed reduction of sulfate?
2. How do you know sulfate was reduced? Please describe in detail below including the chemical reaction involved.
3. What colors indicate reduction of iron? __________________ and ________________.
4. When iron (Fe) is reduced it goes from ____________ to ___________. (form of iron)
5. Reduction of what chemical compound exemplifies the strongest reducing soil conditions? (State the oxidized and
reduced forms)
6. Refer to Figure 11 to answer the following questions.
At the following Redox Potential (Eh) in Mv, and pH ranges, what compounds are being reduced?
Eh = +400, pH = 8 → ____________
Eh = 0, pH = 6 → ____________
Eh = -300, pH = 5 → _____________
Who is responsible for the microbially mediated reactions at the following ranges?
Eh = +500, pH = 9 → ____________________
Eh = 0, pH = 9 → _____________________
Figure 1: “Schematic of process for determining cation exchange capacity” by Colby J. Moorberg and David A. Crouse is
licensed under CC by 4.0
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8.1: Introduction to Soil Health
Introduction to Soil Health
(Adapted from NRCS, 2017)
Soil health is also commonly referred to as, soil quality. As defined by the Natural Resource Conservation Service, soil
health is “the continued capacity of soil to function as a vital living ecosystem that sustains plants, animals, and humans.”
This concept identifies the fact that only “living” things can have health, thereby stressing the concept that a soil is a living
ecosystem. As with all living entities, including living ecosystems, the only way to maintain life is through proper
management.
Soil is not simply an inert growing medium, but rather it is comprised of billions of biological organisms, ranging from
microscopic to macroscopic in size. Together these organisms help to maintain the life of the soil, as well as many
physical, chemical and biological factors. Therefore, soil is an ecosystem which can provide the following capabilities:
(NRCS, 2017)
1. Regulating water - Soil helps control where rain, snowmelt, and irrigation water goes. Water and dissolved solutes
flow over the land or into and through the soil.
2. Sustaining plant and animal life - The diversity and productivity of living things depends on soil.
3. Filtering and buffering potential pol utants - The minerals and microbes in soil are responsible for filtering,
buffering, degrading, immobilizing, and detoxifying organic and inorganic materials, including industrial and
municipal by-products and atmospheric deposits.
4. Cycling nutrients - Carbon, nitrogen, phosphorus, and many other nutrients are stored, transformed, and cycled in the
soil.
5. Physical stability and support - Soil structure provides a medium for plant roots. Soils also provide support for
human structures and protection for archeological treasures.
The capabilities of a soil are determined by both inherent and dynamic properties/qualities within the soil. Inherent soil
quality is, “a soil’s natural ability to function.” An example of an inherent soil property/quality is how a sandy texture soil
drains faster than a clayey texture soil, or how a deep soil has more space for root growth compared to a soil with bedrock
at or near the surface. Essentially the idea behind inherent properties/qualities is that these characteristics do not change
easily. On the contrary, dynamic soil quality is, “how soil changes depending on how it is managed.” Management
decisions and efforts can affect factors such as the amount of soil organic matter, soil structure, and water and nutrient
holding capacity. Thus, dynamic soil quality is directly affected by inherent soil quality.
One major goal of soil health is to manage the soil in a way that enhances or improves soil function. It is important to
understand that soils wil respond differently to management depending on inherent soil properties, and so how these
properties vary across the surrounding landscape should be taken into consideration. Another core goal of soil health is to
assess and manage soil so that it functions optimal y for current and future use. Monitoring changes in soil health is critical
for determining sustainable soil management and practices.
Aggregate Stability
A soil with strong aggregates generally has adequate biological activity, organic matter, and nutrient cycling present. When
soil aggregates are weak, it may be a sign of degradation.
Leaving soil vulnerable to greater risk of wind and water erosion, and impeding infiltration and root growth. Aggregation
is especially important for survival of biological soil communities and will affect the amount of pore space between the
soil particles, therefore also impacting bulk density, CEC, and many other dynamic soil properties.
Soil particles form aggregates as a product of cementing agents in the soil including clay content, adsorbed cations such as
calcium and magnesium, and iron oxide content. Expansion and contraction of clay particles as they become moist and
then dry can shift and crack the soil mass and create aggregates or break them apart. Calcium, magnesium, iron, and
aluminum stabilize aggregates via organic matter sorption. In contrast, aggregate stability decreases with increasing
amounts of exchangeable sodium: dispersion is promoted when too many sodium ions accumulate between soil particles.
NUTRIENT CYCLING
There are several plant-available nutrients that are important to the overall health of a soil. The three major nutrients of
importance are N, P, and K. Nutrients occur naturally in soils in mineral form, through biological inputs, atmospheric
deposition, and the application of fertilizers. Nutrients are lost from systems through processes like runoff, water solubility,
plant uptake and leaching. When managing a soil for plant production, it is important to understand nutrient cycles,
availability, and potential for loss.
4 2
−
The process begins with autotrophic and chemolithotrophic bacteria oxidizing ammonium generated by organic matter
mineralization and fertilizer addition, producing nitrate and acid, leading to soil acidification. The process of nitrification
determines the relative amounts of different inorganic N sources available for plant and crop growth and is responsible for
significant loss of added N fertilizer.
Denitrification involves the reduction of nitrate to nitrite, nitric oxide, nitrous oxide and nitrogen gas. This process is
catalyzed by bacteria, archaea and fungi, and is essential for returning N to the atmosphere.
decompose. Soil organisms consume the organic matter extracting energy and nutrients and releasing water, heat, and CO 2
back into the atmosphere. If organic matter is added to the soil at a faster rate than organisms convert it to CO , C will
2
currently farmers and scientists are interested in reversing that effect by increasing C stored in the soils through
management. (Source: NRCS East National Technology Support Center)
NUTRIENT AVAILABILITY
In addition to nutrient cycling, the actual availability of those nutrients to the plants is critical to consider when assessing
soil health. Certain nutrients are only available under specific soil conditions. Soil pH is especially important when it
comes to nutrient availability. Consider the following figure:
Questions
1. Aggregates of soils high in organic matter are more stable than are those low in OM. Which soil aggregates fall apart
when wetted, and which will maintain their stability?
2. Discuss the positive and negative impacts of tillage on soil structure. What is a physical consideration in deciding
whether to utilize this practice on agricultural land?
3. What are 3 management strategies to support and promote soil aggregation?
N
P
K
N
P
K
S
Fe
Ca
☰ 10
Volume of water used = 30mL + [dry weight of soil x soil water content (g/g)]
**ppm in extract = lb NO3-N/acre / 43.6 = lbs/1000 sq ft
Note: for garden topsoil, bulk density can be assumed to be 1.1 g/cm3 and 1.5 g/cm3 for subsoil. For all other soil textures,
see table below.
Table 1. Soil bulk density according to texture
Soil Textures Avg bulk density (g/cm3)
Sand 1.56
Loamy sand 1.54
Sandy loam 1.5
Loamy sand 1.45
Silt loam 1.2
Sandy clay loam 1.63
Silty clay 1.55
Clay loam 1.45
Silty clay loam 1.4
Note: The maximum nitrate-N reading on the nitrate/nitrite test strip container is 50 ppm. If the sample reading falls into
the 50 ppm category, the sample can be diluted to get a better estimate of the actual amount over 50 ppm. To dilute the
sample, fill the eye dropper with filtered solution and place five drops in a plastic container. Add five drops of distilled
water; mix gently by swirling the container. Take a reading with a new test strip as stated in Step 4. Multiply the estimated
nitrate-N in ppm by 2 before using the calculations. If the nitrate reading falls into the category of 50 ppm again, repeat the
dilution steps, and multiply the estimated nitrate-N in ppm by 4.
Soil sample site description
Bulk Density
N-Availability Questions
1. Using Table 2, determine three vegetables that would have soil-N requirements met based on your soil sample.
2. Compost might typically contain about 1% total N, but only 10% of that becomes available in the first year. So typical
compost would contain 0.1% NO3-N, meaning about 1000 lb of compost is needed for each lb of N to be added, and
substantially increase SOM content. Explain how compost can provide a supply a slow release of nutrients and long-
term benefits to soil health.
Table 2. Nutrient requirements of common vegetables
Calculate:
1. How much N is in a 50 lb bag of 12-22-18?
2. How much P2O5 is in a 40 lb bag of 0-46-0?
3. How much K2O is in a 70 lb bag of 12-22-18?
4. How much N is in a 30 lb bag of 0-46-0?
5. How much N is in a ton of DAP (18-46-0)?
6. How much P is in a ton of DAP (18-46-0)?
Calculating the amount of fertilizer needed to supply a certain amount of N, P2O5, or K2O.
When we calculate the amount of fertilizer needed to supply a certain amount of N, P2O5, or K2O we need to know:
1. How much N, P2O5, or K2O is needed per unit area (e.g., 1,000 square feet, acre, etc.)
2. The analysis (grade) of the fertilizer (e.g., 10-10-10, 0-46-0, etc.)
3. How much area we need to fertilize.
4. For manure applications, you need to know the amount of N that will be available during the growing season. Rule of
thumb is 60%.
You need to apply 2 lbs of N per 1,000 square feet. You have a 30-0-0 grade fertilizer available for application. The area
you need to fertilize is 150,000 square feet. How much fertilizer do you need to apply?
Step 1. Determine the rate of fertilizer needed.
Step 2. Determine the fertilizer analysis (grade).
Step 3. Calculate how much fertilizer you need per 1,000 square feet.
Divide the fertilizer application rate by 0.30 (30%):
Step 4. Calculate how much fertilizer is needed to cover the required area.
Calculate:
1. You need to apply 2 lbs of N per 1,000 square feet. You have a 15-10-10 fertilizer available, and the total area to be
fertilized is 3 acres. How much fertilizer will you need? (Hint: Don’t forget to convert sq. ft. to acres!)
Know and understand the five soil-forming factors and how they influence the formation of local soils.
In the field, determine the soil properties of texture, structure, color, pH, slope %, slope position, water runoff class, and water
permeability.
Learn the major properties of cambic, calcic, and argillic diagnostic horizons.
9.1: OVERVIEW
9.2: CLIMATE
9.3: RELIEF
9.4: TIME
9.5: SOIL CLASSIFICATION
Soils in the United States are classified according to the USDA Soil Taxonomy. Soil Taxonomy includes the system of soil
classification published by the Soil Survey staff of the U.S. Department of Agriculture’s Natural Resource Conservation Service
(NRCS), formerly the Soil Conservation Service (SCS). This system provides a comprehensive natural classification of soils based
upon measurable and observable soil morphological properties.
1 11/8/2021
9.1: OVERVIEW
☰
Soils occur in definite patterns as a consequence of the five soil-forming factors. Short distance changes in the soils are
related primarily to changes in topography and parent material.
Hillslope positions across a landscape Wysocki et al., 2000 and Schoeneberger et al., 2012
Parent Material
When rocks are exposed to atmospheric conditions, they begin to adjust to their new environment. This adjustment (known
as weathering) involves processes which cause physical disintegration and chemical decomposition of the rocks. The
weathering of bedrock produces unconsolidated debris serving as the parent material for soils. The parent materials
undergo continued alteration and evolve into a soil reflecting the integrated effects of climate, biotic factors (plants,
animals, and microorganisms), topography, and time.
Some common parent materials of the Laramie area are alluvium, colluvium, and residuum.
Alluvium Unconsolidated material transported and deposited by flowing water.
O
O
A
A A
C E
Typical profile C Bt
R Bs
R C
C
R
R
Time (yrs.) 0 10 to 1,000 1,000 to 10,000 10,000 to 100,000
The diagnostic subsurface horizons are called endopedons and characterize different soils in the system. Several subsurface
horizons are described in the U. S. Soil Taxonomy. Two of these (Argillic and Cambic) are common in the Snowy Range
Mountains area. Not all soils have a diagnostic subsurface horizon.
Wycolo Series
The Wycolo series consists of moderately deep, well drained soils that formed in materials derived from interbedded
limestone, redbed sandstone, and shale. Wycolo soils are on uplands, structural benches, and strath terraces. Slopes range
from 2 to 20 percent. The soils formed in materials weathered from interbedded limestone, redbed sandstone, and shales.
Elevations are 6,000 to 7,800 feet. The mean annual precipitation is 10 to 14 inches of which at least half occurs in the
spring months. The mean annual temperature is 40 to 45 degrees F., and the frost-free season is 85 to 110 days.
Well drained; runoff is medium; moderate permeability. These soils are used mainly as rangeland. Native vegetation is
western wheatgrass, needle and thread, and big sagebrush.
Read through and complete the following activity and questions. All questions should be answered and completed labs are
due at the end of the laboratory period. No late work will be accepted.
Soil Series Parent Material Rock type Soil Series Parent Material Rock type
Alcova Canwall
Tieside Redrob
Pilotpeak Poposhia
Soil series
Land use
landscape position
Slope %
10.2: HUMUS
Humus is the stable, amorphous, heterogeneous fraction of the soil organic matter remaining after the major portion of added plant
and animal residues have decomposed. It is dark brown to black. It is bound to the surfaces of clays and hydrous oxides, which
renders it resistant to microbial attack. The original source of the organic materials is not easily determined.
1 11/8/2021
10.1: Microbial Activity and the Carbon Cycle
The solid portion of soil is composed of minerals and organic matter. The organic matter includes plant and animal
residues at various stages of decomposition, cells and tissues of soil organisms, and substances synthesized by the soil
biota. Each of these contains many types of compounds including proteins, sugars, polysaccharides such as cellulose,
hemicellulose, and starch, and complex fats, waxes, and lignins. All the lifeessential elements are contained in the
collection of organic compounds found in soils.
The total amount of organic matter in soil and the nutrients it contains vary with the climate, nature of the parent material,
soil pH, and the kind and amount of vegetation produced on or applied to the soil.
The process of soil organic matter decomposition and humus formation may be represented as a partial oxidation process
as follows:
organic substances + O2 + microorganisms + suitable environment ------> CO2 + H2O + Energy (both as heat and for
microbial growth) + humus + new microbial cells + inorganic plant nutrients (Ca2+, Cl-, Co2+, Cu2+, Fe2+, H3BO3, H2PO4-,
K+, Mg2+, Mn2+, MoO42-, NH4+, Ni2+, S2-, and Zn2+)
Under natural soil conditions, organic residues undergo an initial rapid decomposition, which liberates large amounts of
carbon dioxide, water, energy, and releases small quantities of inorganic nitrogen, phosphorus, sulfur and other plant
nutrients. Probably the most important function soil microorganisms serve is to recycle organic carbon to carbon dioxide,
thereby maintaining photosynthesis on the earth.
The microbial oxidation process slowly decomposes plant and animal residues. Nutrients contained in the plant and animal
tissue are transformed from unavailable organic forms to plant-available inorganic forms during the microbial oxidation.
Hence, organic matter acts similar to a very slow-release fertilizer and buffers the soil-plant environment against drastic
fluctuations in available plant nutrients. The complete cycling of carbon through plants, animals, organic residue, humus,
and CO2 eventually occurs.
A soil containing one percent organic matter contains up to 45 million kilocalories of potential energy per acre-furrow-
slice. This energy is equivalent in heating value to about 6 tons of coal, or 31 barrels of crude oil or 168,000 cubic feet of
natural gas. Soil microorganisms use only a small amount of the energy from the decomposition of organic residues for
building new cells. The production of a single pound of soil organic matter from plant residues that have been amended
with fertilizer nitrogen requires the total destruction of perhaps 10 to 20 pounds of plant residues to provide the energy for
the process. Furthermore, whenever fresh residues are applied to soil, the decomposition of "humified" organic materials
already present in the soil is accelerated.
The principal reasons for adding organic residues to soils are to modify the tilth of the soil, making seed bed preparation
easier, to add plant nutrients, and to dispose of unusable or unwanted organic waste. Other benefits of adding organic
matter to soils include improved soil structure, increased cation exchange capacity, increased water holding capacity,
increased aeration, and a reduction in soil erosion.
Plant roots + root hairs 2,500 to 4,000 1.13 x 1013 1.35 x 104
Source: Brady, N.C. and R.R. Weil. 2002. The nature and properties of soils. 13th ed. Prentice Hall, Upper Saddle River,
NJ. †AFS = acre-furrow-slice = 2,000,000 lbs. of dry soil which is assumed to contain about 2.5 % humus. 1 hectare-15
cm = 2,000,000 kg of dry soil which is assumed to contain about 2.5 % humus.
A number of conditions affect microbial populations in soils. The optimum temperature range for decay organisms is
between 70-100 °F (about 20-40 °C). Soil temperatures outside this range will retard the activity of most soil organisms.
Excessive water in soil reduces the numbers and kinds of living organisms due to poor aeration. However, at low moisture
levels soil organisms thrive better than do higher plants. Numbers of fungi, bacteria and actinomycetes vary with soil pH.
If pH of the soil is <6.0, the fungi become the dominant soil microorganisms. The supply of nutrients, organic material for
energy, and free oxygen gas affect microbial numbers. Fortunately, optimum soil conditions for most plants and for most
soil microorganisms are similar.
Microbial decay of organic matter releases copious amounts of carbon dioxide. In this way carbon is cycled from the soil
to the air, completing the carbon cycle that began with photosynthesis. More microbial activity is synonymous with a
greater production of carbon dioxide by soil microbes.
Composting of organic material is one way to manage the overall decay process. This allows time for the microbes to
decompose most of the organic residues.
Read through and complete the following activity and questions. All questions should be answered and completed labs are
due at the end of the laboratory period. No late work will be accepted.
Microbial Observations
1. Carefully retrieve the 3 plastic bottles from the incubation shelf keeping them vertical during the transfer from the
incubation area to your desktop
2. Open each bottle one at a time. Examine the contents of the bottles by performing the procedures indicated below.
1. Move your hand across the top of the bottle bringing the air towards your nose.
2. Smell the air and record the odor of what you smell.
3. Examine the soil surface of each bottle for fungi using first your eyes and then the microscope. Fungi produce
fungal mycelia as tissue that has the appearance of fine cotton threads or spider webs.
3. Rate the appearance of fungal mycelium in the control, alfalfa, and sawdust bottles using the terms none, slight,
abundant, or overgrown.
Odor detected Appearance of fungal mycelium
Control
Alfalfa
Sawdust
Carbon Dioxide
During the microbial incubation, the microbes decomposed the organic amendments and the humus in the soil, releasing
carbon dioxide into the atmosphere of the incubation bottle.
Date experiment was initiated = ____________ Date analyzed = __________________
Number of days of incubation = _____________
Figure 1: Observed increase atmospheric CO2 derived from direct measurements, taking the average of Mauna Loa
(Hawaii) and the South Pole (thin solid line) and two ice cores: Law Dome (dashed thin line) and Siple (thin dotted line).
This is compared to total anthropogenic emissions (thick solid line) and 46% of total emissions (thick dashed line). (Knorr
2009)
5. Increasing temperatures on earth is attributed to an increase in carbon dioxide, which traps heat via the greenhouse
effect. The increased atmospheric carbon dioxide is due to the burning of fossil fuels. Explain why carbon dioxide levels in
the world’s atmosphere has lagged behind emissions. Consider possible carbon sinks that may be keeping carbon in
terrestrial and marine environments.
Procedure
1. After viewing each of the three treatment containers with the binocular microscope, carefully add 50 ml of the 0.01
Molar calcium chloride extracting solution to each of the containers.
2. Using a glass stirring rod, stir mixture for 30 seconds. Rinse stirring rod and return to proper location.
3. Allow containers to sit undisturbed for 15 minutes while soil particles settle.
4. After the settling period, obtain one nitrate strip for each container and carefully tilt the container to about a 45-degree
angle to allow enough depth of solution for dipping of the test strip. Dip the test strip with tab facing down to avoid
floating organic matter. Lightly shake off any excess solution and read the nitrate color from the test strip against the
color chart at one minute after dipping. The color pad at the end of the test strip is the one to read for nitratenitrogen.
5. Cleanup: Place the nitrate test strip into a trash can and thoroughly rinse the plastic container with tap water to remove
all soil and extraction solution, dumping rinse material into bucket in sink. Place plastic container in proper storage
container.
Nitrate Observations
Color of the test strip Amount of nitrate produced (ppm)
Control
Alfalfa
Sawdust
Nitrate Questions
1. Rank the treatments (control, alfalfa, sawdust) in terms of the amount of nitrate produced in each treatment, from
greatest to least amount of nitrate produced.
Immobilization of nitrogen occurs when the carbon to nitrogen ratio (C:N) is high (≥30:1) and an insufficient level of
ammonium and nitrate exist in the soil to meet the needs of the microorganisms that are decomposing the organic matter.
Usually, immobilization occurs when the nitrate level is nearly zero. Immobilization occurs during the initial part of the
degradation of most organic materials in the soil. After a sufficient period of time, the microbes will run out of energy after
they have decomposed most of the organic material and release large amounts of carbon dioxide. Once the microbes have
run low on energy, they begin to release extra ammonium into the soil by the process of mineralization. The ammonium
produced in the soil is rapidly converted to nitrate by the nitrifying bacteria. Consequently, after mineralization, the nitrate
level in the soil amended with an organic material will be higher than was the nitrate level of the original soil.
2. Based on the discussion above, briefly explain the ranking (#1) (the amount of nitrate produced in each treatment).
3. Do your results for the amount of nitrate in each treatment make sense in terms of the C/N ratios of the various
treatments?
4. What would be the effect on plants if a finely ground organic matter having high C/N (85/1 straw) were added to the
soil?
Introduction
Microorganisms play an essential role in cycling elements that make the planet habitable for all other types of organisms. The diversity of these simple life
forms is evident in the myriad of ecological niches they inhabit, from hydrothermal vents to the acidic lining of your stomach.
In this activity, you will observe a sampling of the diverse microorganisms that live in your local environment. Winogradsky columns will be set up-simple
devices for constructing a stratified ecosystem-that illustrate different types of microbial metabolism in a colorful way.
Samples will be set up at the beginning of the semester and you will be required to make observations four times throughout the the spring. Answers to the
questions below are due in Lab 10: Soil Ecology so ensure that you have completed these by that lab period.
Materials
4 clear containers
1-4 disposable containers (plastic baggies) for mixing soil
1 trowel for digging sediment sample
4 small labels
Water
1 bucket container large enough to hold 6-10 cups of soil
Large measuring cup for measuring soil and mixture
A carbon source (organic forest litter); appx 1 cup loosely packed
6-10 cups soil
Sulfur source; raw egg yolk is best
Large mixing spoon
1 funnel
Procedure
Four Winogradsky columns will be set up: a control column and columns that contain added carbon, added sulfur, and carbon and sulfur. Make sure to set aside
a few minutes on each of the assigned observation weeks during the experimental period to make observations and take photos of your columns.
Collect the Soil Sample
1. Identify a soil source in your area-anywhere with soil and water is appropriate, such as a stream, creek, lake beach (sand) or even back yard.
2. Take 2-3 photographs of your sample site to illustrate the location where your sediment was collected.
3. Collect approximately 10 cups of sediment in the bucket. The sample should be wet. Avoid or pick out rocks, sticks and leaves; include some additional
water from the sample site if possible.
Assemble the Winogradsky Columns
1. Label 4 containers: “Control,” “Carbon,” “Sulfur,” and “Carbon and Sulfur.”
2. Label 4 disposable containers corresponding to the plastic containers and add appx 1.5 cups soil to each. Do not cross-contaminate samples.
3. For “Control” column, skip to step 5
4. For the other three containers,
1. For “Carbon” column: Add ½ cup shredded newspaper (loosely packed) to soil and mix with spoon or trowel
The newspaper contains cellulose, a source of carbon
2. For the “Sulfur” column: Add the yolk of a raw egg to the soil and mix. If using hardboiled eggs, crumble the yolks.
Egg yolk is a source of calcium sulfate in the column
3. For the “Carbon and Sulfur” column: Add both nutrients as described in steps a and b.
5. Mix each of the samples thoroughly. Make sure to remove any large debris such as leaves, rocks or sticks. Slowly mix in water until the mixture has a
milkshake consistency.
6. Using a large spoon, slowly add appx 1 cup of the mixture to the appropriately labeled plastic column. As you add the sample, tap the column on the counter
to release any trapped air in the column.
7. Add water on top of the soil until there is a 2 cm layer of water on the surface, and air at the top of the column.
8. Place the lid on each column and turn a half turn. Do not tighten the lid!
9. Place all 4 columns in a well-lit place (window).
10. Using a camera or phone, take a photograph of the columns to document week 0 of experiment.
0
Lab 3
Lab 5
Lab 8
Lab 10
Questions
1. How do your columns differ? How are the similar?
2. Did you observe changes in the control column? If so, explain why they occurred
3. Winogradsky columns form oxygen concentration gradients. Predict the distribution of oxygen throughout the column. Consider the soil, water and air
4. Winogradsky columns form sulfide concentration gradients as well. In the columns that contain egg yolk, predict how sulfide will be distributed throughout
the column.
5. Purple sulfur bacteria and green sulfur bacteria are two types of bacteria that use sulfide to support photosynthesis. In general, green sulfur bacteria tolerate
higher levels of sulfide than purple sulfur bacteria do. Predict where the green and purple sulfur bacteria would be in relation to each other.
6. If samples were extracted from various layers of all the columns, where would you find photosynthetic organisms such as cyanobacteria and algae? Why?
7. Explain how Winogradsky columns illustrate the diversity of microorganisms found on Earth today in terms of the diversity of niches they occupy.
GOAL: To become familiar with soil and the related uses made of these soils in the local area.
Instructions
Form a group of no more than 4 students from the introductory soil science course or work alone.
Identify, locate, and photograph at least 10 of the following items. You will receive 1 point for each item up to 10
points. If you are unsure about any of your photos – take a few extra to ensure you get the full 10 points.
As a group, assemble a document that contains the photographs along with a detailed caption that answers the question
posed in the table below. Your lab instructor may require a presentation, poster, or word document.
Be sure that everyone’s first and last names are on the finished assignment. Ask your lab instructor how to submit this
assignment.
A. Evidence of good or sound land management. What is the land use and why is it considered good management?
B. Evidence of poor land management. What is the land use and why is it considered poor management?
C. Soil structure – from a previously exposed soil profile What type of structure is this and where was the photo taken?
D. Evidence of proper water management What is it and why is this smart water usage?
E. Parent material – a photo from aboveground is fine What is this PM and how do you know this?
F. Rock What is this rock, where did you find it, and how do you know that it is correctly identified?
G. Mineral What is this mineral and how do you know that it is correctly identified?
H. Soil as engineering media Where are you and why is this soil considered engineering media?
I. Soil as agricultural media Where are you and what is being grown in this soil?
J. Evidence of biological activity in the soil What organism is responsible for this activity and is the activity beneficial or harmful and why?
K. Soil organism What is the organism and how did you capture it? How does this organism affect the soil?
L. Soil order – a photo of any soil order taken from
What is the soil order and how can you be sure that it is correctly identified?
aboveground – unless there is an open soil pit or exposed soil
M. Something used in everyday life that is reliant on the soil What is it and why is it reliant on the soil?
You may only photograph 2 items/concerns from each row (i.e. 2 soil orders, 2 parent materials, 2 soil structures…). You will receive an extra point (for a total of 11/10) if you take a photo
of the entire group in front of one of the items!
Agricultural uses
Intensive crop production requires suitable highly productive soils. Cultivation should be avoided on lands not suitable for intensive agricultural production or on highly erodible land.
Future generations expect a continued supply of healthful and affordable food. This will require a sustainable farming enterprise that will insure continued soil productivity for the future.
Increasingly, people are concerned about both the future productivity of the land and about the nature of the overall quality of the soil health (microorganisms and other organisms living in
and on the soil). Best management practices (BMPs) must be followed to assure continued successful agricultural production with minimum consequences (limited nitrate leaching or
pesticide contamination of ground waters). The value of prime farm land must be recognized and appreciated.
In the semi-arid portions of the U.S., erosion due to the wind dominates. In these areas, windbreaks and shelter belts are constructed using trees and shrubs. On the open prairies, wheat is
planted in swaths perpendicular to the wind to reduce soil loss by high winds.
The presence of rare or endangered species on agricultural lands is increasingly pitting farmers and ranchers against those with a concern for the ecosystem and the environment.
Urban Planners
Urban planners need soil information to assist in planning future urban growth and areas for future expansion. This includes considerations for future open space preservation, water
drainage and water recharge locations, and other specific local soil concerns. They must consider the importance of protecting agricultural land as prime farmland for future generations.
Often, urban communities are sensitive to the need for aesthetic conditions such as green belts and parks. Stream beds and poorly drained areas can often be developed for green belts and
avoid the damage created by constructing buildings or highways on these poorly drained areas.
Soil engineering properties are particularly of concern relative to construction sites. Storm water runoff, sewage water treatment, and promoting clean air and water are important soil
functions to consider when making urban plans. The spatial relationships of the soils and the native vegetation patterns are of primary importance relative to parks, playgrounds, golf
courses, poorly drained areas, green belts and other open spaces. An assessment of the local geology and natural resources plus existing highways, railroads, rivers, and other transportation
routes must be included in any long-term plan.
Zoning Regulations
Almost every proposed land use will have some conflicting use as viewed by various members of the community. Consequently, all land use decisions are hotly debated political issues.
These issues usually are described as zoning decisions. Libertarians want the government to have no role in any aspect of their lives. On the other side are various specific interest groups
and environmental groups advocating the government must intervene to protect the soil for future generations. Poorly drained areas (flood plains) must be avoided for most human
habitation. Steep hillsides which are prone to slope failures must be avoided for housing developments. Poorly drained and wet soils are not suitable for individual home septic tanks.
Zoning maps are commonly developed delineating areas with severe or hazardous conditions for potential zoning.
The needs associated with an increase in population and growth must be balanced with the availability of existing and projected expansion of possible resources available to serve this
increase in population. The rights of property owners and farmers to sell their land must be balanced against a higher good to the community to preserve or protect such land for future
generations.
References
Bartelli, L. J., A. A. Klingebiel, J. V. Baird and M. R. Heddleson. 1966. Soil surveys and land use planning. Soil Science Society of America and American Society of Agronomy. Madison,
WI.
Beatty, Marvin T., Gary W. Petersen, and Lester D. Swindale. 1979. Planning the uses and management of land. Agronomy 21. American Society of Agronomy, Crop Science Society of
America, Soil Science Society of America, Inc. Madison, WI.