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Expt 6 Data Sheet

The document describes an experiment to isolate soluble ribonucleic acid (RNA) from yeast. [1] The procedure involves lysing yeast cells with alkali and heat, then centrifuging to separate insoluble residue from soluble RNA in the supernate. [2] Acid is added to precipitate the RNA, which is separated from the supernatant by centrifugation. [3] Ethanol and ether are used to wash and purify the isolated RNA.

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Sweetzeil Tapia
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43 views

Expt 6 Data Sheet

The document describes an experiment to isolate soluble ribonucleic acid (RNA) from yeast. [1] The procedure involves lysing yeast cells with alkali and heat, then centrifuging to separate insoluble residue from soluble RNA in the supernate. [2] Acid is added to precipitate the RNA, which is separated from the supernatant by centrifugation. [3] Ethanol and ether are used to wash and purify the isolated RNA.

Uploaded by

Sweetzeil Tapia
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Group Members: Date Performed:

Score /28
Percentage %
Group No. Section:

Experiment 6
ISOLATION OF SOLUBLE RIBONUCLEIC ACID

OBJECTIVE

_____________________________________________________________________

_____________________________________________________________________

_____________________________________________________________________

DATA/OBSERVATIONS

Describe the RNA obtained: (4 pts)


a. color:
b. texture: _______

QUESTIONS

1. Give the purpose of the following reagents in each step involved in the isolation
procedure performed. (8 pts)

a. alkali

__________________________________________________
__________________________________________________
__________________________________________________

b. acid

__________________________________________________
__________________________________________________
__________________________________________________

c. acidified alcohol __________________________________________________


__________________________________________________
__________________________________________________

d. ether
__________________________________________________
__________________________________________________

1
__________________________________________________

2. The following is an incomplete schematic diagram for the isolation procedure used.
Label each step with the reagents used and products obtained. (16 pts)

Yeast

(a)_______________, heat, centrifuge

residue 1 (discard) supernate 1

A) _____________ (b) _________, pH, centrifuge

residue 2 (discard) supernate 2

B) ________________ evaporate, filter

residue 3 (discard) supernate 3

Cool to 40oC

+ c)________

+ conc. HCl

residue 4 supernate 4

alcohol + d) ___________

residue 5 supernate 5

C) ________________ D) _____________________

Legend:

2
a,b,c,d : reagents added
A,B,C,D : products obtained

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