PROFORMA

RECOGNITION OF LABORATORY UNDER THE WATER

ACT, 1974 & AIR ACT, 1981

(To be filled in by all existing laboratories to be considered for recognition as Water Act, 1974 & Air Act,
1981 by Haryana State Pollution Control Board

1. General

(i) Name of Organization : _______________________________________________

(ii) Name of the Laboratory : ______________________________________________

(iii) Address

a) Postal : ________________________________________________________

b) Telephone : _____________________________________________________

c) Fax : __________________________________________________________

d) E-mail : ________________________________________________________

(iv) Year of establishment of

organization : ______________________________________________________

(v) Year of establishment of

environmental laboratory/wing : _________________________________________

(vi) Type of Organization : (Please tick the

appropriate to your Organization)
Government Autonomous Public Sector

Pollution Control Educational Institute

Board/Committee (Govt./Govt. added/private)

Private NGO Any other

1
(vii) If laboratory/organization is private/NGO, give details:
a. Whether registered with local, state

or central govt. authorities : Yes / No

b.
If yes, mention Registration No. and date : __________________________
c.
Nationality of owner/head of the
Organization
d.
Laboratory is located in (tick relevant)
Commercial/Business complex
Residential area Industrial area
Other
e. Laboratory is situated in authorized/
approved area notified by the govt. Yes / No

(viii) Objectives & scope of the organization*

(Please indicate, among others, whether it includes specialized testing, measurement,

services)

(ix) Head of the Organization :

a) Name

b) Designation

c) Address

d) Telephone

e) Fax No.

f) E-mail

2
(x) Laboratory Incharge, if different than (ix) above.

a) Name and Designation : ____________________________________________

b) Address : _______________________________________________________

c) Telephone: ________________ Fax _______________ E-mail ____________

(xi) Name of accreditation body(s)/organization i.e. ISO, NABL, GLP, SPCB's, PCC's etc.
from which the laboratory has been already recognized/accredited, give details.

S. No. Nam e of the Accreditation / recognition granted Environmental Validity up to

certification/recognition for the activities Parameter covered
body/organization

(xii) If applied for renewal of laboratory recognition under EPA, 1986, give previous
recognition details:

a. Validity period : From ________ to __________

b. Reference of Gazette notification : ________________________________
c. CPCB/MOEF reference No. : ____________________________________

(xiii) Whether laboratory ever been de-recognized before its validity period of recognition
under The Water Act, 1974, The Air Act, 1981 and The E(P) Act, 1986 by State
Pollution Control Board/Pollution Control Committee/Central Government/ CPCB, if
yes, give details:

3
2. Infrastructural details of Laboratory: (please enclose brief lay out plan map of laboratory)
with organizational chart and laboratory position in there to:

(i) Total floor space of the environmental laboratory (in sq. mtr): _____________

a) Water Laboratory = Sq. mtr

b) Biological & Microbiological Laboratory = Sq. mtr

c) Air Laboratory = Sq. mtr

d) Provide scanned photograph of above with layout plan.

(ii) Details of major projects undertaken pertaining to environmental studies:

[Please attach separate sheet, if space is insufficient]

(iii) Which of the following type of analytical tests are being carried out in the laboratory
[please mark Yes (V )/No (x)]:

a) Physical k) Hazardous waste Characterization

b) Inorganics general and non metallic l) Ambient air
c) Inorganic (Trace metals) m) Source emission
d) Organics (General) n) Air Toxics
e) Trace Organics o) Hazardous Air Pollutants
f) Microbiological p) Volatile Organic Carbon
g) Toxicity m) Noise measurement
h) Biological n) Meteorological
i) Hazardous waste o) Vehicular emission/Auto exhaust
j) Soil, sludge, sediment

(iv) Laboratory scientists/chemist or officials are fully conversant for sampling, monitoring,
preservation and transportation [please tick Yes(V)/No(x)j.

(a) Water & wastewater (i) Hazardous Air Pollutants analysis

(b) Hazardous waste (j) Volatile Organic Carbon analysis
(c) Solid waste (k) Noise monitoring
(d) Soil (l) Meteorological monitoring
(e) Municipal waste (m) Source emission
(f) Biomedical waste (n) Auto exhaust monitoring
(g) Ambient air/fugitive (o) On line ambient air quality monitoring
emission
(h) Air Toxics analysis

4
(v) Laboratory scientists/chemists or officials are capable of analyzing desired/
relevant parameters in various types of matrix [please tick [Yes(V)/No (x)]

a. Liquid Samples (water & wastewater)

b. Solid Samples (soil/mud/solid waste/sludge etc.)

c. Semi-solid samples (sludge/slurry)

d. Gaseous samples (Ambient air, source emission, vehicular emission)

(vi) a) Mark the parameters given in Appendix 'A' which can be analyzed in the
laboratory:

b) Mark the equipment given in Appendix 'B' which are available in the laboratory:

c) Mark the glass apparatus/assembly given in Appendix 'C', which are available in
the laboratory.

d) Mark the Instruments given in Appendix 'D' which are available in the laboratory.

e) Mark the methodology employed for analysis in Appendix 'E'.

f) Mark the Air Quality Parameters, which can be analyzed in the laboratory in
Appendix 'F'.

g) Mark the Instruments/equipment given in Appendix 'G'.

h) Give details about instruments/equipment in Appendix 'H'.

i) Give details about the analytical methods adopted in Appendix 'I'.

j) Give details about the facilities available for analysis of specific organic compounds
in Appendix 'J'.

(vii) Which of the methods given below are being followed for the [Tick V ]:

(a) Water and Wastewater Analysis:

1. APHA 2. BIS 3. USEPA

4. ASTM 5. ISO 6. Any other

(b) Air Pollution Monitoring and Analysis

1. APHA 2. BIS 3. USEPA 4. CPCB 5. ASTM

6. ISO 7. Any other

5
(viii) Provide details for participation in inter-laboratory (between laboratories) Analytical
quality control proficiency testing programme during last 5 years. Attach copy of
performance report with the application

Coordinating Agency i.e. CPCB, Period (Month / Percentage of

Parameter covered
WHO, NABL, SPCB/PCC etc. year) performance

6
 (ix) Name, designation and qualifications of staff/officials posted at environmental
laboratory/branch (with expertise in environmental analysis/testing): (Please enclose
separate sheet if space is inadequate)

S. No. Name Designation Qualification Total experience in any. Nature of present job assignment (V only)
Field (years & months) Administrative Supervisory Analysis/
sampling

(x) Details of training programme/s related with the environment field attended within last
five years by the officials working at the laboratory as mentioned
at (ix)

S. No. Name of official/s Training conducted by the Title/topic Duration

institution/organization

7
(xi) Please indicate by asterisk (*) the name/s of personnel (maximum three) & having desired
qualification and experience as mentioned in Annexure-IV to be considered for
nomination as Govt. Analysts. Brief bio-data of these persons should be enclosed as
per annexure-V.
S. No. Name Designation Qualification Experience in years related
with Environmental Analysis

(xii) If applied for renewal of recognition under EPA 1986, please outline steps taken for up
gradation of laboratory (please attach details as annexure) during recognition period
with respect to:

a) Procurement of new sophisticated instrument

b) Addition of new parameters
c) Participation in Analytical Quality Control (AQC) exercise of CPCB.

Signature :(Head of organization) (Head of laboratory)

Full name : _________________________ _________________________________

(In capital letters)

Seal of laboratory

8
 Self-Assessment by the Laboratory

Pre-requisite for Recognition of Laboratories under the Water Act, 1974 & Air Act, 1981

The laboratory should ensure that it fulfills the following essential requirement by itself through
self assessment before submitting an application seeking recognition under Water Act, 1974 &
Air Act, 1981:
(i) Laboratory (Private) is registered by the local govt./State Govt./Central Govt.
(ii) Laboratory has minimum 9 nos. of full time working skilled manpower with following
qualification:
Nos. of
S. No. Qualification Nature of Job
Manpower
1. High School/Intermediate with Science Assistance in sampling & 2
analysis
2. Bachelor's Degree in Basic Science or Sampling and analysis 4
equivalent.
3. Master's Degree in Science or equivalent or Sampling & Analysis. 3
Bachelors Degree in Engineering / Supervision of Analysis
Technology or equivalent or Ph.D.
Total Manpower (Minimum) 9

(iii) Environmental laboratory should have minimum space required as given

below:
a) Water Laboratory = 100 Sq. mtr
b) Air Laboratory = 100 Sq. mtr
c) Water & Air Laboratory = 150 Sq. mtr

(iv) Laboratory should compulsorily meet essential parameter requirement as Appendix A &
F.

(v) Laboratory fulfills minimum requirement of equipment/instrument as Appendix B, D & G.

(vi) Laboratory should analyzed samples adopting any validated methods i.e. USEPA,
APHA, BIS, ASTM, ISO, EU or CPCB only.

(vii) Laboratory must have environmental journals/books/analytical methods for sample

analysis with adequate space.
(viii) Laboratory should have not been revoked the recognition by any SPCB/PCC and Govt.
Department. If revoked, recognition case will not be considered before a period of three
years from the date of revoked.
(ix) Laboratory must have comprehensive facilities, expertise for water or air or both related
parameters.
(x) Laboratory should apply strictly as per the format with desired enclosures.

9
 APPENDIX

LIST OF PARAMETERS BEING ANALYSED

A) Physical Tests : [Please mark Yes(V )/No (x)]

S. No. Mandatory parameter S. No. Secondary parameter

1. Conductivity 1. Flocculation test (Jar test)
2. Colour 2. Odour
3. pH 3. Salinity
4. Fixed & volatile solids 4. Settleable solids
5. Total solids 5. Sludge volume index (SVI)
6. Total dissolved solids
7. Total suspended solids
8. Turbidity
9. Temperature
10. Velocity & discharge Measurement of
industrial effluent stream

Minimum required - All 10 nos. of parameters Minimum required 3 parameters

B) Inorganic [Please mark Yes (V )/No (x)]

(i) General & Non-metallic

S. No. Mandatory parameter S. No. Secondary parameter

1. Acidity 1. Bromide
2. Alkalinity 2. Carbon dioxide
3. Ammonical nitrogen 3. Chlorine demand
4. Chloride 4. Iodine
5. Chlorine residual 5. Sulphite
6. Dissolved oxygen 6. Silica
7. Fluoride 7. Cyanide
8. Total hardness 8. Sulphide
9. Total kjehldal nitrogen (TKN)
10. Nitrite nitrogen
11. Nitrate nitrogen
12. Phosphate
13. Sulphate

Minimum required - All 13 parameters Minimum required- Atleast 3 parameters

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(ii) Trace Metals [Please mark Yes(V )/No (x)]

S. No. Mandatory parameter S. No. Secondary parameter

1. Boron (B) 1. Arsenic (As)
2. Cadmium (Cd) 2. Aluminium (Al)
3. Calcium (Ca) 3. Beryllium (Be)
4. 5. 6. Chromium (Cr) Total 4. Barium (Ba)
Chromium (Cr) Hexavalent 5. Lithium (Li)
Copper (Cu) 6. Manganese (Mn)
7. Iron (Fe) 7. Selenium (Se)
8. Lead (Pb) 8. Silver (Ag)
9. Magnesium (Mg) 9. Strontium (Sr)
10. Mercury (Hg) 10. Tin (Sn)
11. Nickel (Ni) 11. Antimony (Sb)
12. Potassium (K) 12. Cobalt (Co)
13. Sodium (Na) 13. Vanadium (V)
14. Sodium absorption ratio (SAR)
15. Zinc (Zn)

Minimum required - All 15 parameters Minimum required - Atleast 4 parameters

(C) Organics (General) and Trace Organics [Please mark Yes(V )/No (x) and give details at
Appendix J for Trace organics]

S. Mandatory parameter S. No. Secondary parameter

No.
1. Bio-chemical oxygen demand (BOD) 1. Total organic carbon (TOC)
2. Chemical oxygen demand (COD) 2. Adsorbable organic halide (AOX)
3. Oil & Grease 3. Surfactants
4. Phenol 4. Tannin & lignin
5. Pesticide (each) 5. Poly-chlorinated biphenyl (PCB's) each
(i) Organo-chlorine (BHC, DDT, 6. Polynuclear aromatic hydrocarbon (PAH)
Aldrin, Endosulphan) each
(ii) Organo nitrogen-phosphorous 7. Organic Carbon (in solid)
(Malathion, methyl parathion, 8. Carbon/Nitrogen ratio
Chloropyriphos)

Minimum required - All 5 parameters Minimum required - Atleast 3 parameters

D) Microbiological Tests [Please mark Yes(V )/No (x)]

S. Mandatory parameter S. No. Secondary parameter

No.
1. Total Coliform 1. Total plate count
2. Faecal Coliform 2. Enterococcus
3. Faecal Streptococci 3. Coliphage
4. E. Coli

Minimum required - All 4 parameters Minimum required - Atleast 1 parameters

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E) Toxicological Tests [Please mark Yes(V )/No (x)]

S. Mandatory parameter S. No. Secondary parameter

No.
1. Bioassay method for evaluation of 1. Bio-accumulation, bio magnification and
toxicity using fish (90% survival of fish bio-transformation studies
after 96 hrs in 100% effluent) 2. Estimation of the effect at tissue level
3. Measurement of toxicity using Daphnia or
other organism
4. Measurement of toxicity factor using zebra fish
(dimensionless toxicity test

Minimum required - 1 parameter Minimum required - 1 parameter

F) Biological Tests [Please mark Yes(V )/No (x)]

S. Parameter S. No. Parameter

No.
1. Benthic organism identification and count 5. Saprobity Index
2. Macrophytic identification 6. Chlorophyll
3. Planktonic identification count 7. Primary productivity
4. Measurement of various diversity index 8. P/R Ratio

Minimum required - Atleast 3 parameter G) Characterization of

Hazardous Waste [Please mark Yes(V )/No (x)]

S. Parameter
No.
1. Preparation of Leachate (TCLP extract/water extract)
2. Corrosivity
3. Ignibility (Flash point)
4. Reactivity
5. Toxicity
6. Measurement of heavy metals/pesticides in the waste/leachate

Minimum required - Atleast 3 parameters H) Soil/Sludge/Sediment

and Solid Waste [Please mark Yes(V )/No (x)]

S. No. Mandatory parameter S. No. Secondary parameter

1. Boron 1. Ammonia
2. Cation Exchange Capacity (CEC) 2. Bicarbonate
3. Electrical Conductivity (EC) 3. Calcium
4. Nitrogen available 4. Calcium carbonate
5. Organic carbon/matter (chemical method) 5. Chloride
6. pH 6. Colour
7. Phosphorous (available) 7. Exchangeable sodium percentage (ESP)
8. Phosphate (ortho) 8. Gypsum requirement
9. Phosphate (total) 9. H. Acid
10. Potassium 10. Heavy metal
11. SAR in soil extract 11. Magnesium

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S. Mandatory parameter S. Secondary parameter
No. No.
12. Sodium 12. Mechanical soil analysis
13. Soil moisture 13. Nitrate
14. TKN 14. Nitrite
15. Calorific value 15. PAH
16. Pesticide
17. Potash (available)
18. Sulphate
19. Sulphur
20. TOC
21. Total water soluble salt
22. Water holding capacity

Minimum required: All 15 parameters Minimum required: Atleast 10 param eters

Remarks: Besides minimum instruments/equipment facilities laboratory must qualify minimum 5 essential
groups i.e. A to E for water and similarly A to D for air analysis.

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 APPENDIX 'B'

a) LIST OF EQUIPMENT FOR WATER / WASTEWATER ANALYSIS

[Note: Please mark Yes (V )/No(x)]

S.
Equipment Yes/No Nos. available**
No.
BASIC EQUIPMENTS
1. Ice Box/s* (2)
2. Filtration assembly* (1)
3. Heating Mantle
4. Stop watch
5. Hot air oven* (2)
6. Hot plate* (2)
7. Muffle furnace* (1)
8. Standard weight
9. Water bath
10. Thermometer/s* (4)
11. Refrigerator/s big size* 300 litres or above (2)
SPECIFIC EQUIPMENTS
1. Autoclave* (1)
2. Bottom sampler
3. BOD Incubator* (1)
4. Centrifuge* (1)
5. Aquarium for bioassay test* (4)
6. COD Digester with aluminium heating blocks * (1)
7. Colony Counter
8. Depth Sampler
9. Digester with condensers
10. Digestion chamber* (1)
11. Dissolved oxygen sampler
12. Flocculator ( Jar testing apparatus)
13. Flow meter
14. Incubator for bacteriological test* (2)
15. Laminar flow* (1)
16. Magnetic Stirrer with hot plate* (2)
17. Mechanical Shaker
18. Microwave digester
19. TKN Analyzer semi autom atic with aluminum block digester
20. Ultrasonic water bath
21. Vacuum pump* (1)
22. Water purification / distillation assembly* (1)
23. Ekman Dredge
24. Water sampler

14
S.
Equipment Yes/No Nos. available**
No.
25. Oil & Grease sampler
26. Water Testing kit
27. Chloroscope for residual chlorine
28. Any other equipm ent (please attach details on separate sheet)

Besides minimum analytical capabilities, expertise, laboratory must be equipped with these items if seeking recognition with
desired nos. as mentioned against each item.
Provide minimum numbers of items, in case exact numbers are not available.

Certified that all the above equipments are properly of ________________________

__________________ (Name of laboratory) and procurement records/bills of
instruments/equipment are available at the laboratory. The list of instruments / equipment taken
on loan is appended herewith.

Signature of Laboratory Incharge

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 APPENDIX C

a) LIST OF GLASS APPARATUS AND DISTILLATION ASSEMBLIES

[Note: Please mark Yes (V )/No (x)]

S. No. Particulars Yes or No Total nos.

available
1. Fluoride distillation assembly
2. Cyanide distillation assembly
3. Ammonia distillation assembly
4. Water distillation assembly
5. Soxlet extraction assembly
6. Arsenic estimation assembly
7. Phenol distillation assembly
8. Any other (please enclose details on separate sheet)

Remarks: If actual figures are not available give minimum / least nos. available.

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 APPENDIX 'D'

a) LIST OF INSTRUMENTS FOR WATER / WASTEWATER ANALYSIS

[Note: Please mark Yes (V )/No (x)]
S. No. Name of instrument Yes/No Total Nos. **
BASIC INSTRUMENTS
1. Analytical Balance +* (1) 1 mg ||
2. Conductivity Meter* (1)
3. Dissolved oxygen meter
4. pH Meter with combined glass electrode* (1) ||
5. Turbidity meter* (1) ||
SPECIFIC INSTRUMENTS
1. Alpha/Beta Radioactivity Counter
2. Atomic Absorption Spectrophotometer (Flam e) with the following
cathode lamps + (V available HCL)* (1)
(i) Aluminium (iii) Arsenic (ii) Antimony (iv)
Barellium
(v) Barium (vii) Cadmium (ix) (vi) Boron (viii) Calcium
Chromium (xi) Iron (x) Copper (xii) Lithium
(xiii) Lead (xiv) Magnesium
(xv) Manganese (xvii) Nickel (xix) (xvi) Mercury (xviii) Potassium (xx)
Selenium Silver
(xxi) Sodium (xxii) Strontium
(xxiii) Tin (xxiv) Cobalt
(xxv) Vanadium (xxvi) Zinc
(xxvii) Other, pl. specify
3. Atomic Absorption Spectrophotometer with Graphite Furnace and
Hydride Generation System
4. Organic Halogen Analyzer (AOX/TOX)
5. Binocular Microscope
6. Flame Photometer* (1)
7. Gas Chromatograph with following detector*++ (1)
- ECD-NPD
- FID-TID
- FPD
- Other detector
8. Gas Chromatograph with Mass Spectrometer (GC-MS)
9. High Pressure Liquid Chromatograph (HPLC)
10. Ion Chromatograph
11. Inductively Coupled Plasma (ICP) Spectrometer
12. Mercury Analyzer Digital* (1)
13. Portable Analyzer Kit (DO, pH, Temp. Cond.)
14. Precision Balance weighing up to 1 mg* (water / air)
15. Rotary Evaporator* (1)
16. Spectrophotometer (Visible)* or Ultraviolet & visible* (1)
17. Stereo Microscope

* Besides minimum analytical capabilities, expertise, laboratory must equipped with these items if seeking /
applying for recognition with desired nos. as mentioned against each item.
** Provide minimum number of item, in case exact numbers are not available
+ All H.C.L. may not required essentially
++ GC equipped minimum ECD, NPD & FID with capillary column.
- If equipped with ICP Spectrophotometer then AAS is not required essentially.
- Mercury Analyzer Digital may not required essentially, if Mercury is measured 1 ppb or below by AAS/ICP.

17
b) LIST OF SPECIFIC EQUIPMENTS/INSTRUMENTS FOR HAZARDOUS
WASTE ANALYSIS
[Note: Please mark Yes (V) /No (x)]

S. No. Instruments Nos.

Available
1. Bomb colorimeter
2. Elemental analyzer
3. Flash point apparatus
4. Moisture content meter
5. Rotary evaporator
6. Toxicity characteristic leaching procedure (TCLP) extractor
7. Toxic Gas analyzer
8. X-ray fluorescence (XRF) Spectrometer
9. Zero head space extractor (ZHE)

c) MAINTENANCE CONTRACT STATUS OF IMPORTANT SOPHISTICATED

INSTRUMENTS

[Note: Please mark Yes (V )/No (x)]

S. No. Name of the instrument Repair job undertaken on Whether

Annual Maintenance contract / sufficient
emergency call basis spares
available
1. AAS (Flame & Flameless)
2. AOX
3. Total Organic Carbon Analyzer
4. Gas Chromatograph
5. Water purification system
6. Analytical balance
7. Specific ion meter
8. Mercury analyzer
9. UV-Visible spectrophotometer
10. Alpha/Beta Radioactivity Counter
11. Any other

d) REFERENCE MATERIAL (RMS) AND CERTIFIED REFERENCE

MATERIAL (CRMS)

S. No. Availability of RMS/CRMS Parameters (V /X)

Nos. of standards
1. Trace Metals
2. Organo-chlorine pesticides
3. Organo-nitrogen phosphorous pesticides
4. Polychlorinated Biphenyls (PCB's)
5. Polycyclic aromatic hydrocarbon (PAH)
6. Benzene, Ethylene, Toluene & Xylene
7. Dioxins and Furans

18
 APPENDIX 'E'

Note: - Please enclose details on separate sheet, if space is inadequate.

- Provide list of standards (RM/CRM) with their names, make & expiry date.

19
METHODOLOGY EMPLOYED FOR ANALYSIS
[Please tick V relevant adopted method]

(A) PHYSICAL PARAMETERS

S. No. PARAMETER METHOD ADOPTED

1. Colour a. Visible comparison method (only potable waters)
b. Spectrophotometric Method (All)
2. Odour Threshold odour test
3. Conductivity Conductivity Meter
4. pH Value Electronic (pH Meter)
0
5. Total solids dried at 103-105 C Gravimetric
0
6. Total suspended solids dried at 103-105 C Gravimetric
0
7. Total dissolved solids dried at 180 C Gravimetric
0
8. Fixed and volatile solids ignited at 550 C Gravimetric
9. Settleable solids Volumetric using Imhoff concentration
Gravimetric
10. Sludge volume index (SVI) Volumetric followed by gravimetric ( using Imhoff conc. and
filtration device)
11. Salinity a. Electrical conductivity method
b. Density method
12. Settled sludge volume Volumetric
13. Turbidity Nephelometric
14. Temperature Thermometer
15. Velocity and discharge measurement of river, a. Cross-Section-velocity Method
drain, industrial effluent stream etc b. Weirs (Rectangular or V Notch or U-Notch)
c. Chemical Methods.
16. Flocculation test (Jar test) Dosing of coagulants
17. Other Parameters (Please specify)

20
 APPENDIX 'E'

(B) INORGANIC (GENERAL & NON-METALLIC)

S. No. PARAMETER METHOD ADOPTED

1. Acidity a. Electrometric/Potentiometric titration
b. Color Indicator titration
2. Alkalinity a. Electrometric/Potentiometric titration
b. Color Indicator titration
3. Ammonical Nitrogen a. Distillation followed by colorimetric method (Nesselerization or
phenate)
b. Distillation followed by titrimetric method
c. Distillation followed by ion Selective electrode method
4. Bromide Colorimetric (Curcumin or Carmine)
5. Carbon Dioxide a. Titrimetric
b. Nomographic
6. Chloride a. Titrimetric (Argentom etric or Mercuric Nitrate)
b. Potentiometric
7. Chlorine dem and Dosing of sampling chlorine solution
8. Chlorine Residual Titrimetric
9. Cyanide a. Distillation followed by Titrimetric
b. Distillation followed by Colorimetric
c. Distillation followed by Cyanide - Selective Electrode
10. Dissolved Oxygen a. Winkler titrimetric-azide modification
b. Membrane electrode method
11. Fluoride a. Distillation followed by Colorimetric (SPADNS or Alizarin Red)
b. Distillation followed by Fluoride selective electrodes
12 Iodine a. Leuce Crystal violet method
b. Amperom etric titration method
13. Total kjehldal nitrogen a. Macro kjehldal method
b. Semi micro kjehldal method
14. Nitrite nitrogen Colorimetric
15. Nitrate nitrogen a. Colorimetric
b. Cadmium reduction method
c. Electrode method
16. Phosphate Colorimetric
17. Sulphate a. Turbidimetric
b. Gravimetric method with residual/ignition of residue
18. Sulphide a. Iodometric method
b. Ion selective electrode method
c. Methylene blue method
19. Sulphite a. Titrimetric
b. Phenonthralin method
20. Silica a. Molybdosilicate method
b. Heterotopy blue method
21. Total hardness Titrimetric (EDTA method)
22. Other parameters (pl. specify)

21
 II. TRACE METALS (Tick for applicable methods for elemental analysis)

S. Elements Flame atomic Flame atomic Flame Electro thermal Hydride/cold Inductively ICP/MASS Anodic Alternative
No. absorption (direct) absorption photometry atomic vapour atomic coupled plasma Spectrom etry stripping methods
(extracted) absorption absorption (ICP) ICP/MS voltametry (colorimetric/
titrimetric by
difference etc)
1. Aluminium (Al)

2. Antimony (Sb)

3. Arsenic (As)

4. Barium (Ba)

5. Beryllium (Be)

6. Boron (B)

7. Cadmium (Cd)

8. Calcium (Ca)
3
9. Chromium (total) (Cr )
6
10. Chromium (Hexa) (Cr+ )

11. Cobalt (Co)

12. Copper (Cu)

13. Iron (Fe)

14. Lead (Pb)

15. Lithium (Li)

16. Magnesium (Mg)

17. Manganese (Mn)

18. Mercury (Hg)

19. Nickel (Ni)

20. Potassium (K)

21. Selenium (Se)

22. Silver (Ag)

23. Sodium (Na)

24. Sodium Absorption Ratio (SAR)

25. Strontium (Sr)

26. Tin (Sn)

22
27. Vanadium (V)

28. Zinc (Zn)

1. Total nos. of metal analysis claimed _________ ;

2. Metal digestion method adopted (pre treatment (please tick appropriate)

(a) Using hot plate

(b) Closed loop system
(c) Microwave digestion

23
C. ORGANIC (GENERAL) & TRACE ORGANICS
[Please mark Yes (V) /No (x) for adopted method]
S. No. PARAMETER METHOD
GENERAL
0
1. Bio-chemical Oxygen Demand (BOD) a. Three days BOD at 27 C
0
b. Five days BOD at 20 C
2. Chemical oxygen demand (COD) a. Open reflux method
b. Closed reflux titrimetric method

c. Closed reflux colorimetric

3. Oil & Grease a. Grass metric (simple extraction)

b. Soxhlet extraction

4. Phenol a. Distillation followed by colorimetric

b. Chloroform extraction

5. Adsorbable organic halogens Adsorption pyrolysis titrimetric

6. Organic carbon (in solids) Rapid titrametration method
7. Total organic carbon a. High temperature combustion
b. Persulphate ultraviolet or heated persulphate oxidation

c. Wet oxidation method

8. Surfactants a. Surfactant separation by sublation

b. Anionic surfactants as MBAS

c. Non imic surfactants as CTAS

9. Carbon/Nitrogen Ratio By calculation

10. Tannin & lignin Colorimetric method
TRACE ORGANICS
11. Pesticides a. Organo-chlorine (Please specify adopted method)
b. Organo-phosphorous (Please specify adopted method)

c. Carbamates (Please specify adopted method)

d. Herbicides (Please specify adopted method)

e. Fungicides (Please specify adopted method)

12. Polychlorinated biphenyl (PCB's) Please specify adopted method

13. Poly nuclear aromatic hydrocarbon Please specify adopted method
14. Volatile Organics Please specify adopted method
15. Trihalomethanes Please specify adopted method

D. MICROBIOLOGICAL TESTS (Adopted method)

S. No. PARAMETER METHOD
1. Total coliform a. Multiple tube technique
b. Membrane filter technique
2. Faecal coliform a. Multiple tube technique
b. Membrane filter technique
3. Faecal streptococci a. Multiple tube technique
b. Membrane filter technique
4. Enterococcus a. Multiple tube technique
b. Membrane filter technique
5. Total plate count a. Pore plate method
b. Spread plate method
c. Membrane filter method
6. E. Coli a. Multiple tube technique
b. Membrane filter technique
7. Others (Please specify)

E. HAZARDOUS WASTE PARAMETERS (Adopted method)

S. No. PARAMETER METHOD
1. Preparation of Leachate (TCLP extract/water extract -
2. Determination of various parameter in Leachate i.e. Methods as prescribed in water analysis
metal, pesticides etc.
3. Corrosivity a. Electrometric (by pH meter)
b. Corrosivity toward steel
4. Reactivity Identification of characteristic properties i.e. explosive, reading
violent, violently react with water forms potential explosive mixture
with water etc.
5. Ignitability a. By Pen sky martens apparatus
b. By seta flash closed cap tester
6. Toxicity Toxicity characteristics leaching procedure (TCLP)
7. Other (Please specify)

24
 APPENDIX

AIR QUALITY PARAMETERS

Facilities available [Please mark Yes (V )/No (x)]

A. Ambient Air / Fugitive Emissions

S. No. Group of parameter Yes/No Adopted method

S. No. Group of parameter (V / x)
Yes/No Adopted method
(i) Mandatory Parameters (V / x)
1.
(i) Nitrogen
Mandatorydioxide as NO2
Parameters
1.
2. Particulate matter
Sulphur dioxide (SO2)
2.
3. Sulphur dioxide particulate matter
Total suspended
3.
4. Velocity & flow
Respirable suspended particulate matter (PM10)
4.
(ii) Carbon dioxide
Secondary Parameters
5.
1. Carbon
Ammoniamonoxide
6.
2. Temperature
Carbon monoxide
7.
3. Oxygen
Chlorine
8.
4. Oxides
Fluorideof nitrogen
(ii)
5. Secondary Parameters
Non methane hydrocarbon
1.
6. Acid
Leadmist
2.
7. Ammonia
Methane
3.
8. Chlorine
Ozone
4.
9. Fluoride
Benzene(Particulate)
Toluene Xylene (BTX)
5.
10. Fluoride
Polycyclic(Gaseous)
aromatic hydrocarbon (PAH) Benzo-a-pyrine
6. Minimum required
Hydro-chloric acid - At least 4 parameters from secondary parameters

Minimum required - At least 5 parameters from Secondary parameter

B. Stack gases/source emission

25
C. Noise level

S. No. Group of parameter Yes/No Adopted method

(i) Mandatory Param eters

1. Noise level measurement (20 to 140 dba)

2. Ambient Noise & Source specific noise

D. Meteorological Monitoring

S. No. Group of parameter Yes/No Adopted method

(i) Mandatory Parameters
1. Ambient Temperature
2. Wind direction
3. Wind speed
4. Relative Humidity
(ii) Secondary Parameters (Minimum required at least
one parameter)
1. Solar radiation
2. Rain fall

E. Vehicular Emission Monitoring

S. No. Group of parameter Yes/No Adopted method

(i) Mandatory Parameters
1. Carbon monoxide
2. Smoke Density
3. Hydrocarbon
(ii) Secondary Parameters (Optional)
1. Oxides of Nitrogen

Remark: Laboratory seeking recognition must qualify minimum 4 groups A to D groups of parameters with
appropriate space requirement, skilled manpower and adequate infrastructure facilities.

26
 APPENDIX-G

LIST OF EQUIPMENT/INSTRUMENTS
[Please mark Yes (V)/No (x)]

If yes, give Total

S. No. Instrument Yes/No
Nos. **
1. BTX analyzer (PID/FID detector)

2. BTX calibrator

3. Charcoal Tubes

4. CO Analyzer (Non-dispensive Infrared principle)

5. Detector Tubes with Pump of different pollutants (Please specify details)

6. Dust analyzer (Beta Attenuation/TOEN)

7. Exhaust CO/HC analyzer

8. Flue gas analyzer

9. Gas Chromatograph with Air sampling port, FID & PFPD detectors

10. Handy sampler for gaseous monitoring* (2)

11. High Volume sampler with flow controller (4)

12. Low flow pump

13. Meteorological sensors with mast (W S, WD, Temp., Humidity)* (1)

14. Micro balance (Readability 1 ug)

15. Multi calibration system

16. Multi channel recorder

17. Multi calibration kit (portable)

18. Noise level meter* (2)

19. NO-NO2-Nox Analyzer (Chemiluminescence based)

20. Ozone analyzer (Ultraviolet)

21. Permeation tubes for calibration

22. RSPM sampler with flow controller/brush less motor + calibration kit* (4)

23. Smoke density meter

24. SO2 Analyzer (Pulsed Fluorescence based)

25. Soap bubble meter

26. Stack monitoring kit with High Temp. Probes* (2)

27. Toddler Bags

28. Wet gas meter

29. Any other (please specify)

LIST OF INFRASTRUCTURAL EQUIPMENT/ITEMS FOR AIR ANALYSIS

[Please mark Yes (V)/No (x)]

If yes, give Total

S. No. Items Yes/No
Nos.**
1. Air conditioner (split type)

2. Air Conditioner (Window type)

3. Breathing apparatus

4. Cold room far sample storage

5. Computer with printer

6. Constant voltage transformer

7. Face shield and helmet

8. Gas mask

27
 APPENDIX-G

9. Refrigerator (frost free, CFC free)

10. Tool Kit (Electrical & Mechanical)

11. Uninterrupted power supply (UPS) system

12. First aid box

13. Trolley for sample transportation

14. Fume Hood

15. Exhaust System

16. Fire Extinguisher

17. Electricity Generator

18. Gas Cylinder Trolleys

19 Any other (please specify)

** Provide minimum numbers of items, in case exact numbers are not available
* Besides minimum analytical capabilities, expertise laboratory must equipped with these items, if seeking / applying for recognition with
desired numbers as mentioned against each item.

28
 Appendix H

Details of equipment/instruments available in the Laboratory

(Please enclose separate sheet if space is inadequate)
Procurement Standard operating
S. Measuring Month & year Month & year placed in Calibration Status
Instrument/Equipment Make/Model document/bills procedure (SOP's) Accuracy % ±
No. range of purchase service Internal/External
available (V/x) available (V/x)
1. AAS

2. GC

3. Flame photometer

4. Mercury analyzer

5. BOD incubator

6. Analytical balances

7. Autoclave

8. pH meter

9. Conductivity meter

10. Bacteriological incubator

11. Spectrophotometer (visible)

12. Turbidity meter

13. Noise level meter

14. High volume sampler

15. Stack monitoring kit

16. RSPM sampler

17. Meteorological sensor

(Please provide details on separate sheet, if space is inadequate) * If

external, mention date of calibration validity.

29
 Appendix I

METHOD DETAILS OF TEST PARAMETERS MARKED AS V AT APPENDIX A TO F

(Please enclose separate sheet, if space is inadequate)

Method adopted (Please

Measuring SOP's Available
S. No. Parameter provide method details viz. Minimum Detection Limit (MDL)
(V /x)
Range
Method Nos., page details)
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.

30
 Appendix J

Please provide the name of compounds being analyzed in the laboratory using Gas Chromatography
technique for the following groups:

Pesticides Polynuclear Benzene

Polychlorinated aromatic Dioxins & ethylene
S. No. Organo-chl Organo Nitro Trihalomethanes
Carbonates Biphenyls (PCB's) hydrocarbon Furans toluene &
orine phosphorous PAH Xylene

31
 ANNEXURE - III

Attachments

1. Provide coloured scanned photograph showing inner view / work area of the laboratory
for the following sections.

Water and Wastewater Section Microbiology Section

Instrumentation Section Air and Emission Testing Section

Library / Conference Room

Outer view of the laboratory building

2. Enclose Layout Plan of the laboratory with the application.

32
 TERMS & CONDITIONS FOR RECOGNITION OF LABORATORIES UNDER THE ENVIRONMENT
(PROTECTION) ACT, 1986

The following terms and conditions shall be observed for recognition of laboratories under Section 12
(1) (b) of the Environment (Protection) Act, 1986.

1. The laboratory (Private/NGOs) shall be legally identifiable and registered with an appropriate
statutory body i.e. local govt., state govt. or central govt.

2. The laboratory shall perform all the functions as mentioned in Rule 9 of the Environment
(Protection) Rules, 1986.

3. It shall carry out the tests as per the method prescribed by the Central Government of any
authorities constituted under Section 3 (3) of the Environment (Protection) Act, 1986 from time
to time.

4. The test report shall be recorded in Form III of the Environment (Protection) Rules 1986 in
triplicate. It shall be signed by the Government Analyst and be sent to the officer from whom the
sample is received by the laboratory.

5. It shall carry out those tests, which are specified in APPENDIX-A to F of the application and it
shall not carry out any other test on the samples given.

6. The laboratory shall charge rates not exceeding those fixed for EPA recognized laboratories.

7. The laboratory shall not charge rates higher than the rates they charge to any other
Government or Public Sector organization.

8. The laboratory shall ensure that a sample submitted to it for testing will only be tested by a
person, recognized as 'Government Analyst' by Central Government under provisions of the
Environment (Protection) Act and as notified in the official gazette from time to time.

9. When a Government Analyst ceases to be in the services of the Laboratory, the Head of the
Laboratory shall report this fact to the Central Government within fifteen days and
simultaneously take steps for filling up this vacancy.

10. Any report signed by the Government Analyst may be used as evidence of facts in a court of
law as per Section 1 4 o f the Environment (Protection) Act, 1986. The laboratory shall provide
all facilities to the 'Government Analyst' for giving evidence in a court of law, if it becomes
necessary.

11. It shall maintain complete secrecy in respect of the test results. These shall not be divulged to
any person or authority other than the Officer empowered under Section 11 of the Act of the
court having jurisdiction.

33
 ANNEXURE - III

12. Laboratory shall remain open for all working days except weekly off, Central & State Govt.
holidays. Environmental laboratory of an educational institute/college will make arrangement of
acceptance of samples and their analysis during any vacation exceeding more than 5 days i.e.
summer/winter vacation etc.

13. It shall maintain proper records and registers and the calculations and test results in respect of
tests conducted by them.

14. The laboratory and the Government Analysts employed by the laboratory shall participate in
(Analytical Quality Control Exercises) organized by the Central Government or an organization
designated by it to test the capabilities of the recognized laboratories and analysts from time to
time. The fee if so, for AQC exercise has to be paid by the participating laboratory to the
designated organization.

15. If feel necessary, Central Government will send dummy environmental samples to the
laboratory to keep constant check over the laboratories of the results of the sample, which are
to be analyzed, free of cost by the laboratory and results will be provided to the Central
Government.

16. If the laboratory is sent samples from an establishment with which it has got connections
through ownership or other means which make it improper for the laboratory to carry out the
tests with respect to that sample, it shall disclose the fact to the empowered officers or authority
sending the sample and shall refuse the samples.

17. It shall be the responsibility of the laboratory to maintain properly the necessary infrastructure
for conducting tests successfully.

18. In case the laboratory desires to make a mention of its recognition as environmental laboratory
in its letter heads, printed material, signboards, etc., it shall specify the period of recognition
and such mention of the recognition shall cease immediately after the expiry of recognition.

19. The laboratory shall comply with all the rules and regulations notified under the Environment
(Protection) Act, 1986.

20. The recognition shall become effective from the date of its Gazette Notification up to a period of
five years or revocation whichever is earlier.

21. The Central Government / CPCB shall have the right to de-recognize the laboratory at any time
in public interest without assigning any reason, if it is deemed necessary by the Central
Government.

22. Private/NGO's laboratory shall maintain complaint register (bounded and numbered) having
the following columns

Customer's name and Ref. No. if any Date on which Name of Complaint has been
address sample received complainer rectified in the
laboratory

34
23. The recognition accorded to Government Analysts in an environmental laboratory ceases along
with the de-recognition of that environmental laboratory.

24. Each of environmental sample test report provided by the private laboratory to the customer
must give in their footnote regarding availability of complaint register with the owner.

25. If the laboratory has shifted within city from the place, where it has been granted recognition
earlier the laboratory will inform Central Govt. regarding the change as well as.

26. In case of take over of a recognized private laboratory, its ownership changes; occurrence of
such changes must be communicated to the recognition body MOEF/CPCB within one month.
Through, an appropriate mechanism that the laboratory continues to comply with the criteria
against which recognition was originally granted will be verified.

27. After recognition, laboratory can be re-inspected at any time for its periodic
assessment/performance.

The aforesaid terms and conditions are acceptable to us.

Dated: Signature _______________________________________________________

(Head of Laboratory)

Full Name ____________________________

(in capital letters)
Address _____________________________

Seal of laboratory
Signature of the Head of the Laboratory

35