LEARNING ACTIVITY SHEET

QUARTER 2nd / SEMESTER 1st, Week 2

Name: ______________________________________________ Score: _______

Grade & Section: _________________________ Subject: GENERAL BIOLOGY 1

Name of Teacher: __________________________________Date: _____________

I. Title: Photosynthesis (Light and Dark Reaction)

II. Type of Activity: Concept notes with formative activities
LAS for summative assessment ( Written Work Performance

Task)

III. MELC: Describe the patterns of electron flow through light reaction events
(STEM_BIO11/12-IIa-j-4); and Describe the significant events of the Calvin cycle
(STEM_BIO11/12-IIa-j-5).

IV. Learning Objective/s:

 Explain the events that occur in light reaction.
 Discuss the events of the light-independent reactions of photosynthesis
(Calvin Cycle).
 Realize the importance of photosynthesis as one of the most vital processes
of life.

V. Reference/s:
Print Material/s:

Reece, J. B., Urry, L. A., Cain, M. L., Wasserman, S. A., Minorsky, P. V., and
Jackson, R. B. (2011). Photosynthesis. In Campbell biology (10th ed., pp.
195-199). San Francisco, CA: Pearson.

Online Resource/s:

Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., and Walter, P. (2002).
Chloroplasts and photosynthesis. In Molecular biology of the cell (4th
ed.). New York, NY: Garland Science. Retrieved
from http://www.ncbi.nlm.nih.gov/books/

1
 Berg, J. M., Tymoczko, J. L., and Stryer, L. (2002). A proton gradient Across
the thylakoid membrane drives ATP synthesis. Retrieved
from http://www.ncbi.nlm.nih.gov/books/NBK22519/

Berg, J. M., Tymoczko, J. L., and Stryer, L. (2002). Two photosystems generate
a proton gradient and NADPH in oxygenic photosynthesis. Retrieved
from http://www.ncbi.nlm.nih.gov/books/NBK22538/.

Biologydictionary.net Editors. (2016, November 10). Calvin Cycle. Retrieved

from https://biologydictionary.net/calvin-cycle/

Gunther, T. (2020). Calvin Cycle. Retrieved from.

https://www.nationalgeographic.org/ media/calvincycle/

VI. Summative Assessment

A. Multiple Choice. Read and analyze the questions carefully. Please choose the
letter of your answer.

1. The cellular process of ___1___ requires chemical energy produced by

____2___.

1 2
a. photosynthesis respiration
b. net primary production decomposition
c. decomposition photosynthesis
d. biosynthesis respiration

2. Over a plant's lifetime ____1____ of the energy converted from sunlight to

the energy of sugar is lost as ____2____.

1 2
a. 10% water
b. 90% heat
c. all oxygen
d. 10% carbon dioxide

3. Energy is said to flow through the ecosystem rather than cycle because it:
a. is passed from one trophic level to the next.
b. is destroyed by each organism as it is used.
c. never returns to be re-used by the autotrophs.
d. increases in supply as it moves up the food chain.

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4. Which organ of the plant does photosynthesis most commonly occur?
a. stem
b. leaves
c. roots
d. flowers

5. The light dependent reactions of photosynthesis occur in the:

a. thylakoid membranes
b. stroma
c. mitochondrion
d. outer surface of the chloroplast

6. When plants do not receive enough water, their photosynthetic rate drops
significantly. This is because:
a. water is a raw material needed for the light dependent reactions.
b. the stomates close and carbon dioxide is not available.
c. sugar builds up and inhibits photosynthesis.
d. not enough oxygen is produced to keep glycolysis running.

7. The sugar produced by photosynthesis is used:

a. to produce ATP in respiration.
b. as raw material for biosynthesis.
c. to make new DNA.
d. for all of the above

8. Plant seeds germinated in complete darkness will continue to loose biomass

and eventually die. The loss of biomass occurs in the form of ________ that is
lost from the plants.
a. water
b. oxygen
c. carbon dioxide
d. sugar

9. The picture below shows several organisms in a marine ecosystem. Which

organism produces oxygen?

a. Corals
b. Algae
c. School of fish
d. Water

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10. During the energy-capturing reactions of photosynthesis, which of the
following occurs?
a. light-dependent reactions
b. light-independent reactions
c. both a and b

11. ATP production during photosynthesis is sometimes called

photophosphorylation because __________.
a. light is involved
b. water is involved
c. oxygen is involved
d. all of the above

12. All of the following are main factors affecting photosynthesis except?
a. Light intensity
b. Carbon dioxide concentration
c. Temperature
d. Color of the leaf
13. Which of the following is the correct reaction of photosynthesis?
Light
a. 6CO2 + 12H2O C6H12O6 + 6HO2 + 6O2
Chlorophyll

Light
b. 6CO2 + 12H2O C6H12O6 + 6O2 + 6O2
Chlorophyll

Light
c. 6CO2 + 12H2O C6H12O6 + 6O2 + 6H2O
Chlorophyll

Light
d. 6CO2 + 12H2O C6H12O6 + 6CO2 + 6O2
Chlorophyll

14. It is a grouping of pigment molecules and proteins.

a. Electron
b. Photons
c. ATP
d. Photosystem
15. The energy that these molecules carry is stored in a bond that holds a single
atom to the molecule. For ATP, it is a phosphate atom, and for NADPH, it is a?
a. hydrogen atom.
b. Oxygen atom
c. Carbon atom
d. ATP

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 B. TRUE or FALSE. Write T if the statement is true or F if the statement is false.

_____1. Because water is such an important component of photosynthesis, a

shortage of water can slow or even stop a reaction.

_____2. Temperature is also a factor that affects photosynthesis.

Photosynthesis is dependent on temperature. As the enzymes
approach their optimum temperatures the overall rate increases.
Above the optimum temperature the rate begins to decrease until it
stops.

_____3. The intensity of light does not affect the rate of photosynthesis.

_____4. Increasing light intensity decreases the rate of photosynthesis.

_____5. After the light intensity reaches a certain level, the plant reaches its
maximum rate of photosynthesis.

_____6. Carbon dioxide concentration affects the rate at which photosynthesis

occurs, as it increases the rate at which sugars are produced in a light-
dependent reaction as the concentration itself increases.

_____7. Whichever factor is in the most limited supply, or the limiting factor,
affects the productivity of the reaction more than the amount of the
other reactants in the reaction.

_____8. The concentration of Chlorophyll also affects the rate of

photosynthesis, as the rate of light being absorbed would change if
the amount of chlorophyll present changed.

_____9. Pollution also affects photosynthesis to some degree, as soot in the

air can block stomata, and reduce the transparency of leaves.

_____10. A limited amount of water in a reaction will not limit the quantity of
carbon dioxide present in the reaction, limiting the rate of
photosynthesis.

C. Fill in the Blank. Supply the missing word/s to complete the given statements
on photosynthesis.

1. _________energy-capturing portion of photosynthesis that takes place in

thylakoid membranes of chloroplasts and cannot proceed without solar energy, it
produces ATP and NADPH.

2. ________ green pigment that absorbs solar energy and is important in

photosynthesis.

3. ______________ membrane-bounded organelle with chlorophyll – containing

membranous thylakoids; where photosynthesis takes place.

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 4. _______________Process usually occurring within chloroplasts whereby
chlorophyll traps solar energy and carbon dioxide is reduced to a carbohydrate.

5. ____________Synthesis portion of photosynthesis that takes place in the stroma

of chloroplasts and does not directly require solar energy; it uses the products of
the light dependent reactions to reduce carbon dioxide to a carbohydrate.

D. Identification/Explanation. Explain the following questions.

1. What happens to water, CO2, and sunlight in photosynthesis, meaning what is
created from these reactants?

2. What is NADPH? What is the difference between NADP+, NADPH, ADP, and ATP?
How does ATP turn into ADP+P?

3. Write the chemical equation for the process of photosynthesis.

4. What are the reactants and products of dark reaction? Where in the chloroplast do,
they occur?

5. What is Light-Independent Reaction often called?

6. Where does the carbon dioxide come from? What will happen to it and what will it
eventually become?

7. How many molecules of high-energy sugars are produced as a result of one Calvin
Cycle?

8. Draw a picture of a molecule of ATP and an ADP molecule (make sure to KNOW
the differences between these models). Also, label: adenosine, ribose, and
phosphate groups. Finally, show the chemical bond that would be broken if energy
needed to be released (10 points).