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Bouin'S Fixative (Bouin'S Fluid) : Technical Data Sheet 866

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Sreejith R Menon
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85 views1 page

Bouin'S Fixative (Bouin'S Fluid) : Technical Data Sheet 866

Uploaded by

Sreejith R Menon
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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U.S.

Corporate Headquarters Polysciences Europe GmbH


400 Valley Road Handelsstrasse 3 D-69214
Warrington, PA 18976 Eppelheim, Germany
1(800) 523-2575 / (215) 343-6484 +(49) 6221-765767
Fax: 1(800)343-3291 Fax: +(49) 6221-764620
Email: [email protected] Email: [email protected]
www.polysciences.com

Technical Data Sheet 866


Page 1 of 1

Bouin’s Fixative (Bouin’s Fluid)


DESCRIPTION
Bouin’s fixative consists of picric acid, formaldehyde and acetic acid. The primary use of Bouin’s fixative is for lymph nodes, prostate biopsies and kidney biopsies
however, it can also be used for decalcifying bones with the addition of formic acid. Bouin’s fixative preserves tissue detail well and will not destroy morphology
when the bone specimen is left in solution for several days or weeks. The addition of 25% ethanol to the Bouin’s can be used for adipose rich tissues, such as
breast. Bouin’s fix stops the chromogenic substrates very effectively and also helps remove some of the trapped background stain. It stains tissue yellow. This
yellow stain can be removed with multiple washes in buffered ethanol or PBS-Tween. Current protocols suggest that several changes of 70% ethanol are used to
wash the tissue until the yellow color disappears. Do not use Bouin’s to fix before in situ since unable to detect RNA at all. Fix small specimens for at least 6 hours,
and other specimens can be fixed up to two days. Large specimens can be fixed for up to three days. The specimen is usually kept in Bouin’s for 24 hours and
then transferred to 70% alcohol. If the tissue is still yellow in the paraffin block, put the hydrated sections in something weakly alkaline to remove residual picric
acid, then was well with water before staining. Retained picric acid interferes with some stains, including those used for blood cells. Bouin’s fixative is the primary
mordant solution in the trichrome stains.

If red blood cells are of interest, don’t use Bouin’s fluid for fixation because it damages them. Use neutral, buffered formaldehyde instead.
CAUTION: CARCINOGENIC CONTAINS FORMALDEHYDE, IRRITANT, TOXIC

REAGENTS: Bouin’s Fixative Formaldehyde - Severe eye and skin irritant. Sensitizer by skin and respiratory
Saturated picric acid - 3000.0 ml contact. Toxic by ingestion and inhalation. Target organ effects on respiratory
Formaldehyde - 1000.0 ml system. Corrosive. Carcinogen. Label with FORMALDEHYDE WARNING label.
Glacial acetic acid - 200.0 ml Acetic Acid - Target organ effects respiratory system (Corrosive)

Purpose Storge and handling


Bouin’s is generally used for testicular fixation because it preserves nuclei Storage
and chromosomes especially observes well during meiosis. Formalin is a Store at room temperature
more general fixative, used widely for other tissues when mitotic or meiotic Handling
cycles are not necessary to observe. Also used as a mordant for staining Glove, chemical goggles & fume hood
procedures.
Reference
1. Carson,F, Histotechnology: A Self-Instructional Text, pp 19, 1st ED, 1992, ASCP Press
FIXATION TIME 2. Crookham,J, Dapson,R, Hazardous Chemicals in the Histopathology Laboratory, 2nd ED,
For small biopsies fix in 2-4 hours, for large specimens may remain in fixa- 1991, Anatech
tive for a maximum of 72 hours

Ordering Information
PROCEDURE
1. Fixed tissue may be retained in 10% formalin or 70% alcohol Cat. # Description Sizes
2. Prior to staining, remove the picric acid from the tissue by 16045 Bouin’s Fixative (Bouin’s Fluid) 1L, 4G
a) washing in tap water
b) grades of alcohol (50%) Order online anytime at www.polysciences.com
c) or 70% alcohol saturated with lithium carbonate.

Safety To ORDER
Work in a well ventilated area, wear gloves, lab coat and goggles. Avoid In the U.S. Call: 1(800) 523-2575 • (215) 343-6484
contact and inhalation. Picric acid can become explosive if allowed to dry In the U.S. Fax: 1(800) 343-3291 • (215) 343-0214
out. Toxic through skin exposure. Germany Call: +(49) 6221-765767
Germany Faxl: +(49) 6221-764620

Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this
bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond
our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any
government regulation.
Technical Data Sheet 866 © Polysciences, Inc. - REV: 07.22.2010

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