Introductory Lab

Question 1: Which of the solutions below is a strong acid?

✓ H2SO4sulforic acid
– HNO2Nitrous acid
– HCNHydrogen cyanide
– HFHydrofluoric acid

Question 2: How can we calculate the equilibrium concentration of [H3O+]hydronium of a weak

acid?
✓ Use the ICE chart
– It is half of the pKa value
– It is half of the weak acid's initial concentration
– It is always the same as the weak acid's initial concentration

Question 3: What is the Brønsted-Lowry definition of base?

✓ Proton acceptor
– Electron pair donor
– Produces OH-hydroxide in solution
– Contains OH- hydroxide

Question 4: What is the pH of a 0.15 M NH4OH solution with a Kb of 1.78 x 10-5molar ammonium
hydroxide solution with a base dissociation constant of one point seventy eight times ten to the
power of minus five?
✓ 11.21
– 11.93
– 12.09
– 10.85

Question 5: What do you think will happen if you add a hypertonic solution to the cells?
✓ The cells will shrink
– Nothing will happen to the cells
– The cells will disappear
– The cells will swell

Question 6: What do you think will happen if you add a hypotonic solution to the cells?
✓ The cells will swell
– Nothing will happen to the cells
– Half of the cells will explode
– The cells will shrink

Question 7: So, which blood type does our patient belong to?
✓ A 'A' positive
– O positive
– AB negative
– AB positive
– O negative
– B positive
– A 'A' negative
– B negative

Question 8: So, which blood type does our patient belong to?
✓ A 'A' negative

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 – O positive
– AB negative
– AB positive
– B negative
– B positive
– O negative
– A 'A' positive

Question 9: So, which blood type does our patient belong to?
✓ B positive
– O positive
– AB negative
– AB positive
– B negative
– A 'A' negative
– O negative
– A 'A' positive

Question 10: So, which blood type does our patient belong to?
✓ B negative
– O positive
– AB negative
– AB positive
– B positive
– A 'A' negative
– O negative
– A 'A' positive

Question 11: So, which blood type does our patient belong to?
✓ AB positive
– O positive
– AB negative
– B negative
– B positive
– A 'A' negative
– O negative
– A 'A' positive

Question 12: So, which blood type does our patient belong to?
✓ AB negative
– O positive
– AB positive
– B negative
– B positive
– A 'A' negative
– O negative
– A 'A' positive

Question 13: So, which blood type does our patient belong to?
✓ O positive
– AB negative
– AB positive
– B negative
– B positive
– A 'A' negative

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 – O negative
– A 'A' positive

Question 14: So, which blood type does our patient belong to?
✓ O negative
– O positive
– AB negative
– AB positive
– B negative
– B positive
– A 'A' negative
– A 'A' positive

Question 15: Now we need to prepare a buffer that has a pH of 7.4. We need this specific pH to
maintain our blood cells in culture. How should you prepare this buffer?
✓ By mixing weak base with its conjugate acid and then adjusting the pH with strong acid
– By mixing strong acid and then adjusting the pH with strong base
– By mixing strong base and then adjusting the pH with strong acid
– By mixing weak acid with its conjugate base and then adjusting the pH with strong base

Question 16: Which hazard sign is warning you of the potential corrosiveness of strong acids or
strong bases?

Click the VIEW IMAGE button to open the image.

✓ A 'A'
–D
–C
–B

Question 17: Let's begin with some experiments!

What do you think will happen, if we add a strong acid into a beaker of water?
✓ The strong acid will completely dissociate into [H+]protons and [A-]conjugate base
– None of the strong acid will dissociate
– Only a small part of the strong acid will dissociate into [H+] protonsand [A-]conjugate base
– The strong acid will mostly dissociate into [H+]protons and [A-]conjugate base

Question 18: What is the formula to calculate pH?

✓ pH = -log[H3O+] pH equals the negative logarithm of the hydronium ion concentration
– pH = log[H2O] pH equals the logarithm of the water molecule concentration
– pH = log[H3O+] pH equals the logarithm of the hydronium ion concentration
– pH = -log[H2O] pH equals the negative logartihm of the water molecule concentration

Question 19: What is the pH of a 0.00125 M HCl molar hydrochloric acid solution? Remember that
hydrochloric acid is a strong acid.
✓ pH = -log[H3O+]= 2.9 pH equals the negative logarithm of the hydronium ion concentration
which equals 2.9
– pH = -0.00125
– pH = 0.00125
– pH = log[H3O+]= -2.9 pH equals the logarithm of the hydronium ion concentration which
equals negative 2.9

Question 20: What is the pH of the 0.00125 M NaOH molar sodium hydroxidesolution? Remember
that NaOHsodium hydroxide is a strong base.

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 ✓ pH = 14 - pOH = 14 - 2.9 = 11.1equals 14 minus pOH, consequently 14 minus 2.9 equals
11.1
– pH = -0.00125
– pH = 0.00125
– pH = pOH -14 = 2.9 - 14 = -11.1equals pOH minus 14, consequently 2.9 minus 14 equals
negative 11.1

Question 21: Which of the following properties does NOT influence the rate of diffusion?
✓ Color of the particle
– Mass of the particle
– Size of the particle
– Temperature

Question 22: Water potential is the potential energy for water to move from one area to another.
Water always moves from a high water potential area to a lower water potential area. According to
the water potential formula, which factors affect the water potential?
✓ Pressure and the amount of solute
– Surface area and depth
– Speed and concentration
– Volume and temperature

Question 23: The solute potential can be calculated by ΨS = - iCRT negative i times C times R
times T, where i is the ionization constant, C is the molar concentration, R is the pressure constant
(0.00831 liter bars/(mole oK))0.00831 with units of liters times bars divided by moles times degrees
Kelvin and T is the temperature in oKkelvin. Calculate the solute potential of a 2.0 M molar sucrose
solution at 20oC 20 degrees Celsius under standard atmospheric conditions. Take into account that
sucrose doesn't ionize in water, so its ionization constant (i) is 1.
✓ -4.87 bars
– -5.48 bars
– -45.37 bars
– -3.32 bars

Question 24: The substance that is dissolved in a mixture is called?

✓ A solute
– A colloid
– A suspension
– A solvent

Question 25: The substance in which something is dissolved to make a solution is called?
✓ A solvent
– A colloid
– A suspension
– A solute

Question 26: What is the most common solvent in cellular activities?

✓ Water
– Isotonic
– Glucose
– Plasma

Question 27: How is it called when the concentration of a solute inside and outside of the cell is
equal?
✓ Isotonic
– Hyptertonic

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 – Hypertonic
– Hypotonic

Question 28: What are membranes like the cell membrane that allow some molecules to pass
through it called?
✓ Semipermeable
– Permissive
– Actively permeable
– Permeable

Question 29: We need to take a blood sample. What should we do first?

✓ Use ethanol to disinfect the skin
– Measure his temperature
– Place a bandage on the patient
– Directly take the blood using the vacutainer

Question 30: What is the blood group of a person who has antigen A 'A' in their blood?
✓ A 'A'
– AB
–O
–B

Question 31: What is the blood group of a person that has neither antigen A 'A' nor B, and is
positive for antigen D?
✓ O positive
– AB negative
– O negative
– AB positive

Question 32: Which antibodies can be found in a person with blood group O?
✓ Antibodies for both antigen A 'A' and B
– Neither antibody for antigen A 'A' and B
– Antibody for antigen AB
– Antibody for antigen O

Question 33: Which bloodtype can a person with blood group AB positive receive if they need a
blood transfusion?
✓ All of those
– AB positive and AB negative
– B positive and B negative
– A 'A' positive and A 'A' negative

Question 34: There are hazards associated with handling human or animal material, such as blood
samples. Which symbol warns you for potential biological hazards?

Click the VIEW IMAGE button to open the image.

✓B
–D
–C
– A 'A'

Question 35: What kind of protection should you use, when you are working with biohazard level 1
materials - for example human blood samples for routine blood typing tests.
✓ Gloves and some sort of facial protection

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– A positive pressure personnel suit is needed
– No special protection is needed, not even gloves
– Work needs to be undertaken in a biological safety cabinet

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