MPOB Test Method p2.2: 2004 impurities METHODS OF TEST FOR PALM OIL AND PALM : DETERMINATION OF Metin ot PRODUCTS: 1 Scope ‘Tis test species @ method for the determination of insoluble impurties content of palm oil and pat oll products ‘hese impures include mechanical impurties, minerals Tostences, venous resins, calckim soaps, oxidised fatty Sikal soaps, hydroxy-fatty acids and their glycerides substances, carbohydrates, nitrogenous ‘acids, fatty acid lactones, and (in part) {Fis not desired to include soaps (particularly calcium soaps) or oxidised fatty acids in the insoluble impurties content, itis necessary to use a different solvent and procedure. In this case, the method should be the subject of agreement between the parties concerned. 2 Referenced documents ‘The following method contains provisions which, through reference in this text, constitute provisions of this MPOB Test Method. For dated reference, subsequent amendments to or revision of, ny of these publications do not apply. However, parties to agreements based on this MPOB Test Method are encouraged to investigate the possibilty of applying the most recent ‘edtion of the referenced documents. For undated references, the latest edition of the document referred to applies. MPOB Test Methods pt 1004 - Sampling of palm oil and palm oil products. MPOB Test Methods p1.2: 2004 — Preparation of test samples. This method is technically equivalent to ISO 663 Animal and vegetable fats and oils — Determination of insoluble impurities. 3 Definition For the purpose of this MPOB Test Method, the following shall apply. M Impurities Impurities are defined as those materials insoluble in rhexane or light petroleum under the Conditions specified in this method. NOTE 1: The content is expressed as peroentage by mass. NOTE 2: These impurties include mechanical impurties, mineral substances, carbohydrates, nitrogenous Suttons vance wee, alvum spe, ondies fat acs, fat acd lactones, and pat} aka sans, fatty acids and their glycendes. 167MPOB Test Method po... * 2 Impuriet 4 Principle oived in n-hexane or ight petroleum, is fitered and the residue 4, rhe A test prion ofthe ol dasved in nena St 0g°C and weighed. washed with same solvent. 5 Reagents 64 Petroleum ether (60/80°C) or n-hexane. The solvent must be fitered throug, Whatman glass fibre GF/B or its equivalent. 6 Apparatus Usual laboratory apparatus and, in particular, the following. 4 Porcelain Gooch crucible, of internal base diameter 20 mm. 62 ‘Whatman glass fibre fitter GF/B or its equivalent. 63 Electric oven, capable of maintaining a temperature of 103 °C +2 °C. 64 Vacuum fitter flask, 1 L capacity with adaptor and ring for the Gooch crucible. 6s Conical flask, 250 mL capacity. 68 Desiccator, containing an efficient desiccant. 7 Sampling Its important thatthe laboratory receives a sample that is resentat damaged or changed during transport or storage, ‘ety rep ae ae ae ee ‘Sampling is not part of the method ‘sampling method is given in MPOB, ‘est Matod pt. 12004, > TOst Method. recomended Preparation of sample for analysis 168MP ‘OB Test Method p2.2: 2004 Impurities jeigh, to the nearest 0.01 12, 5) and 900 100 mL of sohvent fiter the soluton through the Gooch cruciby fos cart "), several 10 mL le Srert (5. Portions, to transfer al the (i sant fe (6.1) and wash with Several portions of 1 the oll and insoles acum. Use fresh oe Om sovent unt a the oa romance CO Wipe clean with tissue paper and 43, coon the desiccator 66 to ream Temperate #4 content inthe cven (3) at 09 °C Weigh the Gooch crucible and contents to 4 the SZeminatons on the same sample, Nearest 0.001 9. Cary out two 10 Expression of results pe insoluble impurities expressed as percentage by mass is: ma- my a x 100 m where, m__ isthe mass of the test portion, in grams; im _ isthe mass of the Gooch crucible plus fiter pape, in grams; and is the mass of Gooch crucible plus fiter paper plus impurdes,n grams m provided that the requirement for Take as the result the arithmetic mean of two determinations, repeatabilty is satisfied. Report the result to the second decimal place. " Precision mieten Appendix A. ma ry tests on the precision of method oe surat ee tA Tae a rteriaboratony Minese interaboratory teats 2 ot be 8 get and matrices other than those gVe"- 14 Rey peatability ae red using the le test resutts, obtain nent The absolute difference between two independent singe tes ert reno es See eetce maton in ofterent BDO ven a Sevomert yan nck more than So 0.10% (by mass) of insoluble impurte® 169MPOB Tost Method p22: 295, Impurities 41.2 Reproducibility ‘The absolte diference between two independent single test resuits, obtained using the ce in diferent laboratories with different operators using tere ‘method on identical test meters! equipment, Senna tnt mre ofa cas be greater than 008% for Spl cota f2b0ut 0.10% (by mass) of insoluble Impuries ing 12 Test report ‘The test report shall specty pling was carried out, f known; (@) the method in accordance with which sam o the method used; the test result(s) obtained: and tthe repestabilty has been checked, the final quoted resu obtained. details not specified inthis MPOB Test Method, or regarded ax teodents which may have influenced the test resut(s). © @ It shall atso mention all operating ‘optional, together with detaits of any i ‘The test report shall include all infomation necessary for the complete identification of the sample. 170MPOB Test Method p2.2: 2004 Impurities Appendix & (informative) Results of intert laboratory toate bor x insoluble wean kernel oil organised by the Federation oa aay li fa fave (FOSA) and carried Out in accordance with iSO g7zg!, Seed# and Fata = sre esuts are shown in Tables A. to A.3 Table A.t — Intertaboratory test on palm ol og eso elaboratony test ce T Number of laboratories participating 12 Number of nationalities # er results a Aa by mace) C028 Repeatability imit r, % (by Mass) 5.007 Reproducibility imit R, % (by mass) oa Table A.2 — interlaboratory test on crude palm oll Year of interlaboratory test 1688 Number of laboratories participating 27 ‘Number of nationalities a4 ‘Number of accepted results. 25 Mean values, % (by mass) 0.015 Repeatability limit r, % (by mass) 9,008. Reproducibility limit R, % (by mass) 0.026. Tabie A.3 - interlaboratory test on palm kernel oll ‘Year of interlaboratory test ‘e Huber ot aberatones parispating [7] Number of nationalities: A [Number of accepted results 0.017 ean values, % (by mass oor {Reeetabity it % (by Fas). 0.088 uciblty imit R, % (by mass nn '05%25, 1866 (now wingrawn), was used to obtain the precision dats 1 7m