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LC Ms Based Metabolomics and Metabolite Identification With The Q Exactive™ Plus and Orbitrap Id X™ Tribrid™ PDF

This document outlines the schedule and sessions for a training course on metabolomics. The course covers topics like sample preparation, mass spectrometry, data processing, statistics, and metabolite identification. It includes both lectures and hands-on laboratory sessions over four days.

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ChristinaMitsi
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48 views3 pages

LC Ms Based Metabolomics and Metabolite Identification With The Q Exactive™ Plus and Orbitrap Id X™ Tribrid™ PDF

This document outlines the schedule and sessions for a training course on metabolomics. The course covers topics like sample preparation, mass spectrometry, data processing, statistics, and metabolite identification. It includes both lectures and hands-on laboratory sessions over four days.

Uploaded by

ChristinaMitsi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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LC-MS Based Metabolomics and Metabolite

Identification with the Q Exactive™ Plus and


Orbitrap ID-X™ Tribrid™
Course Programme

Date Time Session

09.15 – 09.30 Registration


Lecture: Introduction to the Course and Birmingham Metabolomics
09.30 – 09.40 Training Centre
Prof Mark Viant / Dr Ralf Weber
Lecture: Introduction to Metabolomics
09.40 – 10.00
Prof Mark Viant
Lecture: Introduction to Liquid Chromatography, Mass Spectrometry, and
10.00 – 10:45 the Q Exactive Plus & ID-X Mass Spectrometers
Dr Martin Jones

10.45 - 11.00 Break

Lecture: Sample Preparation in Metabolomics


11.00 – 11.30
Dr Andy Southam
Day 1
Lecture: Importance of Experimental Design
11.30 - 12.00
Prof Mark Viant

12.00 – 12:45 Lunch


Group 1 Lab Session: Sample
Group 2 Lab Session: UHPLC-MS
Preparation (tissue, biphasic
12.45 – 14.45 Analysis Setup and Calibration
extractions)
Dr Martin Jones
Dr Andy Southam

14.45 – 15.00 Break

Group 2 Lab Session: Sample


Group 1 Lab Session: UHPLC-MS
Preparation (tissue, biphasic
15.00 – 17.00 Analysis Setup and Calibration
extractions)
Dr Martin Jones
Dr Andy Southam
Group 1 Lab Session: Sample Group 2 Lab Session: Sample
Preparation (biofluids, Reconstitution and UHPLC-MS Analysis
09.00 – 10.30 monophasic extractions and QC of Profiling Samples Including QC Set-
preparation) up
Dr Andy Southam Dr Martin Jones
10.30 – 10.45 Break
Group 1 Lab Session: Sample
Group 2 Lab Session: Sample
Reconstitution and UHPLC-MS
Preparation (biofluids, monophasic
10.45 – 12.15 Analysis of Profiling Samples
extractions and QC preparation)
Including QC Set-up
Dr Andy Southam
Dr Martin Jones

12.15 – 13.00 Lunch


Day 2
Lecture: Introduction to Metabolomics Data Processing
13.00 – 14.00
Dr Ralf Weber
Computer Workshop: Metabolomics Data Processing
14.00 – 15.30
Dr Ralf Weber

15.30 – 15.45 Break


Lecture: Metabolomics Data Exploration - Introduction to Univariate
15.45 – 16.15 Statistics
Dr Gavin Lloyd

Computer Workshop: Metabolomics Data Exploration - Univariate


16.15 – 17:00 Statistics
Dr Gavin Lloyd

Lecture: Metabolomics Data Exploration - Introduction to Multivariate


09.00 – 09.30 Statistics
Dr Gavin Lloyd
Computer Workshop: Metabolomics Data Exploration - Multivariate
09.30 – 10.30 Statistics
Dr Gavin Lloyd

10.30 - 10.45 Break

Lecture: Basic Concepts - Metabolite Annotation and Identification


Day 3 Dr Ralf Weber
10.45 – 12.15 Lecture: Overview of Instrumentation and Their Potential in Metabolite
Identification
Dr Martin Jones / Dr Andy Southam

12.15 - 13.00 Lunch

Group 1 Lab Session: Use of the


Group 2 Lab session: Use of the QE-
ID-X for Metabolite
13.00 – 14.45 plus for Metabolite Identification
Identification
Dr Andy Southam
Dr Martin Jones
14.45 – 15.00 Break

Group 1 [continued] Lab Group 2 [continued] Lab Session: Use


Session: Use of the ID-X for of the QE-plus for Metabolite
15.00 – 16.45
Metabolite Identification Identification
Dr Martin Jones Dr Andy Southam

Group 1 Lab Session: Use of the


Group 2 Lab Session: Use of the ID-X
QE-plus for Metabolite
09.00 - 10.30 for Metabolite Identification
Identification
Dr Martin Jones
Dr Andy Southam

10.30 - 10.45 Break


Group 1 [continued] Lab Group 2 [continued] Lab Session: Use
Session: Use of the QE-plus for of the ID-X for Metabolite
10.45 - 12:15
Metabolite Identification Identification
Dr Andy Southam Dr Martin Jones
12:15 – 13:00 Lunch
Day 4
Lecture: Introduction to Processing, Filtering, and Annotating
13:00 - 13:45 Fragmentation Data
Dr Ralf Weber
Computer Workshop: Metabolite Annotation
13.45 – 14.45
Dr Ralf Weber / Dr Martin Jones

14:45 – 15:00 Break


Q&A: Tips and Tricks from the Experts and Question Session
Prof Mark Viant, Dr Ralf Weber, Dr Martin Jones, Dr Gavin Lloyd, Dr Andy
15:00 – 16:00
Southam

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