C101-E070L

UV-VIS Spectrophotometers Accessories

UV-VIS Series Accessories

 In order to make full use of the impressive functionality offered by UV-VIS-NIR spectrophotometers, optimal accessories must be
selected based on the given application field or sample characteristics.
Therefore, Shimadzu offers an extensive selection of accessories for a wide range of applications, from basic measurements such as
transmittance, relative reflectance, and absolute reflectance to multianalyte and micro-volume sample measurements.

Compatible Instrument Model Compatible Older

Accessory P/N UV-1280 UV-1800 UV-2600 UV-3600 Plus SolidSpec Instrument Model* Page
UVmini UV-1900 UV-2700 UV-3600
Cells See text ✓ ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6) 5
Film Holder 204-58909 ✓ ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Rotating Film Holder 206-28500-41 ✓ ✓ ✓ (2)(3)(4)(5)
6
Didymium Filter 202-30242-09 ✓ ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Holmium Filter 202-30242-05 ✓ ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Basic Measurement Four-Cell Sample Compartment Unit with Holder 206-23670-91 ✓ ✓ ✓ ✓ (1)(2)(3)(4) (6)
206-69160-41 ✓ ✓ ✓ (2)(3)(4)
Multicell Sample Compartment (Six Cells)
206-60605-42 ✓ (1) (6)
7
Sample Compartment Unit 206-60184-07 ✓ (1) (6)
Cell Type Sample Holder 207-21637-41 ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Glass/Film Holder for Standard Sample Compartment 207-21573-41 ✓ ✓ ✓ ✓ ✓ (2)(3)(4)(5)
Simplified Near
(4)
Infrared Light PMT, R5108 206-29869-41 For UV-2700
(UV-2500 series only)
Measurement
8
1 mm 204-21473-03 ✓ ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Short Optical Path
Spacers for Short-Path Cells 2 mm 204-21473-01 ✓ ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Measurement
5 mm 204-21473-02 ✓ ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Four-Cell Type Long-Path Rectangular Cell Holder 204-27208 ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Long Optical Path Reference-Side Long-Path Rectangular Cell Holder 204-28720 ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
9
Measurement Long-Path Rectangular Cell Holder 204-23118-01 ✓ ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Cylindrical Cell Holder 204-06216-02 ✓ ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
206-14334 ✓ ✓ ✓ ✓ (2) (4)(5)(6)
Super-Micro Cell Holder
206-14334-01 ✓ (1) 10
Micro Cell Holder with Mask 204-06896 ✓ ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Micro-Volume 3 μL Capillary Cell Kit for Ultramicro Volume Measurement 206-69746 ✓ ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Measurement 8/16 Series Micro Multi-Cell Holders and Cells MMC-1600 206-23680-58 ✓ ✓ ✓ (2)(3)(4)
8/16 Series Constant-Temperature Micro Multi-Cell 11
MMC-1600C 206-23690-58 ✓ ✓ ✓ (2)(3)(4)
Holders and Cells
Micro Cell Mask for Standard Six-Cell Holder 206-66828 ✓ (1) (6)
Constant-Temperature Cell Holder 202-30858-44 ✓ ✓ ✓ ✓ (1)(2)(3)(4) (6)
12
Constant-Temperature Four-Cell Holder 204-27206-02 ✓ ✓ ✓ ✓ (1)(2)(3)(4) (6)
Constant-Temperature Water Circulator NTT-2200P ** 208-97263 ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Constant-
Six-Cell Thermoelectrically Temperature-Controlled
Temperature CPS-100 206-29500-XX ✓ ✓ ✓ ✓ (1)(2)(3)(4) (6) 13
Cell Positioner
Measurement
Thermoelectrically Temperature-Controlled Cell Holder TCC-100 206-29510-XX ✓ ✓ ✓ ✓ (1)(2)(3)(4) (6)
Thermoelectric Single Cell Holder S-1700 206-23900-XX ✓ ✓ ✓ (2)(3)(4) (6)
14
Analysis System TMSPC-8 206-24350-XX ✓ ✓ ✓ (2)(3)(4)
160L 206-23790-51 ✓ ✓ ✓ ✓ (1)(2)(3)(4) (6)
160T 206-23790-52 ✓ ✓ ✓ ✓ (1)(2)(3)(4) (6)
Sipper Units 15
160C 206-23790-53 ✓ ✓ ✓ ✓ (1)(2)(3)(4) (6)
160U 206-23790-54 ✓ ✓ ✓ ✓ (1)(2)(3)(4) (6)
N 206-23890-51 ✓ ✓ ✓ ✓ (2)(3)(4)
Syringe Sippers
CN 206-23890-52 ✓ ✓ ✓ ✓ (2)(3)(4)
Auto Sample Changer ASC-5 ** 206-23810-XX ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5) 16
Automatic Analysis Sample Waste Unit SWA-2 206-23820-58 ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Solenoid Valve (Fluoropolymer) 204-06599-01 ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
10 mm 204-06222 ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Micro Flow-Through Cell with Holder
5 mm 204-06222-41 ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6)
Front Panel with Holes 204-27588-03 ✓ ✓ ✓ ✓ (1)(2)(3)(4) (6)
17
Flow Cell for Liquid Chromatography 206-12852-41 ✓ ✓ ✓ (2)(3)(4)(5)
206-80880-41 ✓ (2)(3)(4)
UV Automated System Connection Kits
206-80880-42 ✓ ✓ ✓
Instrument Validation Low-Pressure Mercury Lamp Unit 206-28300-58 ✓
Crossflow Cell for Process Monitor System 206-53570-13 ✓ ✓ (4) 18
Onsite Measurement
Optical Fiber Coupler 206-54175-41 ✓ ✓ (4)

* Compatible Older Instrument Model: (1) UV-1200 series (2) UV-1601/1601PC/1650PC (3) UV-1700 PharmaSpec
(4) UV-2401PC/2501PC/2450/2550 (5) UV-3101/3101S/3101PC/3150 (6) MultiSpec-1500
For details about models older than those shown above or their compatibility, contact your Shimadzu sales representative.
** Corresponding product is not sold in Europe since it is not a RoHS compliant product.
*** Measurement using the Automatic X–Y Stage is not supported.

2
 Compatible Instrument Model Compatible Older
Accessory P/N UV-1800 UV-2600 Page
UVmini UV-1280 UV-3600 UV-3600 Plus SolidSpec Instrument Model*
UV-1900 UV-2700
Specular Reflectance Attachment (5° Incident Angle) 206-14046-58 ✓ ✓ ✓ ✓ ✓ ✓ ✓ (1)(2)(3)(4)(5)(6) 20
ISR-2600 206-28400-58 ✓
ISR-2600Plus 206-28410-58 For UV-2600 21
ISR-240A ** 206-23860-91 ✓ (4)
ISR-3100A ** 206-23851-92 ✓ (5)
Integrating Sphere Attachments ISR-603 207-20100-58 ✓
22
ISR-1503 207-20900-58 ✓
ISR-1503F 207-21300-58 ✓
LISR-2100 ** 206-23862-91 ✓ (5)
LISR-3100 ** 206-23862-92 ✓ (5)
MPC-2600A 207-23520-41 ✓
Multipurpose Large-Sample 23
MPC-3100 ** 206-23831-91 ✓ (5)
Compartments
MPC-603A 207-23550-41 ✓
Powdered Sample Holder (for Integrating Sphere) 206-89065-41 ✓ ✓ ✓ ✓ (4)(5)
Micro Beam Lens Unit 206-22051-41 For UV-2600 ✓ ✓
Micro Sample Holder 206-28055-41 For UV-2600 ✓ ✓
Suspension and
D25 mm 207-23559-41 For UV-2600 ✓ ✓ 24
Opaque Sample
Measurement Cylindrical Sample Holders D50 mm 207-23559-42 ✓ ✓ ✓ ✓ (4)(5)
D110 mm 207-23559-43 ✓ ✓ ✓ ✓ (4)(5)
ASR-3105 206-16817-58 ✓ ✓ ✓ ✓ (4)(5)
Absolute Specular ASR-3112 206-16100 ✓ ✓ ✓ ✓ (4)(5)
Reflectance Attachments ASR-3130 206-15001-58 ✓ ✓ ✓ ✓ (4)(5)
ASR-3145 206-15002-58 ✓ ✓ ✓ ✓ (4)(5)
BIS-3100 206-17059-58 ✓ ✓ (4)(5)
Sample Base Plate BIS-603 207-21100-58 ✓
Integrating Sphere Sets BIS-3700 206-20880-51 For 3700 25
BIS-3700DUV 206-20880-52 For 3700DUV
Large Polarizer Set 206-15694-40 ✓ ✓ ✓ ✓ (4)(5)
Type I 206-13236-41 ✓ ✓ ✓ ✓ (4)(5)
Polarizers Type II 206-13236-42 ✓ ✓ ✓ ✓ (4)(5)
Type III 206-13163-40 ✓ ✓ ✓ ✓ (4)(5)
Polarizer Adaptor Set 206-15693 ✓ ✓ ✓ ✓ (4)(5)
Variable Angle Measurement For MPC-2600A 207-23490-41 ✓
26
Unit For MPC-603A 207-23490-42 ✓
Automatic X–Y Stage 206-20810-58 ✓
DDU 206-20264-51 For 3700
Direct Detection Units
DDU-DUV 206-20264-52 For 3700DUV
27
Special Purge Box 206-21788-58 For 3700DUV
Accessories for Large Specular Reflectance Attachment
SolidSpec-3700 206-20570-58 ✓
(5° Incident Angle)
Variable Angle Measurement For 100V 207-23470-41
✓
Unit For 230V 207-23470-42
Square Cell Holder for Integrating Sphere 206-22339-92 ✓
DPU-414 ** 206-55215-XX ✓ (3) 28
Screen Copy Printers
Printer, DPU-S445 207-23484-48 ✓ ✓
Interface Cable Analog Signal Output Interface 206-25233-91 ✓ ✓ ✓
USB Interface Cable 088-50602-49 ✓ ✓
Water Analysis Program See text ✓ 29
(English) 207-21250-92
LabSolutions DB Connection Kit ✓ ✓ ✓ ✓ ✓ ✓ (2)(3)(4)(5)
(Chinese) 207-21250-93
(English) 207-21251-92 30
LabSolutions CS Connection Kit ✓ ✓ ✓ ✓ ✓ ✓ (2)(3)(4)(5)
(Chinese) 207-21251-93
Optional Tm Analysis Software 206-57476-91 ✓ ✓ ✓ ✓ (2)(3)(4)
Software Film Thickness Measurement Software 206-66877 ✓ ✓ ✓ ✓ (2) (4)(5)
Color Measurement Software See text ✓ ✓ ✓ ✓ (2) (4)(5) 31
Solar Transmittance Measurement Software 206-23130-92 ✓ ✓ ✓ (5)
LabSolutions UV-Vis (English/Chinese) 207-24525-92 ✓ ✓ ✓ ✓ *** 32
(English) 207-24526-92 ✓ ✓ ✓ ✓ ***
LabSolutions DB UV-Vis 34
(Chinese) 207-24526-93 ✓ ✓ ✓ ✓ ***

3
 Guide to Selecting Accessories

Solid Samples
Samples Measurement Method and Conditions Accessories
Standard Sample Compartment + Film Holder, Cell Type Sample Holder,
Less than 3 mm thick
Glass/Film Holder for Standard Sample Compartment
More than 3 mm thick Integrating Sphere Attachment (ISR-2600, ISR-2600Plus, ISR-603)
Transmittance measurement
Requires a large integrating sphere Integrating Sphere Attachment, 150 mm Dia. (ISR-1503, ISR-1503F)
(due to ISO compliance and other reasons).
Large sample size (over 100 mm square) Large-Sample Compartment (MPC-2600A/603A or SolidSpec-3700) Glass Sample Holder for MPC series/SolidSpec
Relative specular Normal measurement Specular Reflectance Measurement Attachment (5° incident angle)
reflectance
Smooth measurement Large sample size (over 100 mm square) SolidSpec-3700 + Large Specular Reflectance Measurement Attachment (5° incident angle)
Surface Absolute Specular Reflectance Attachment (ASR-3105) (Requires a Large-Sample Compartment and
Samples* 5° incident angle measurement
Absolute specular BIS-3100/3700/603 Sample Base Plate Integrating Sphere Set separately).
reflectance 12°/30°/45° incident angle Absolute Specular Reflectance Attachment (ASR-3112, ASR-3130, ASR-3145) (Requires a Large-Sample
Reflectance measurement measurement Compartment, BIS-3100/3700/603 Sample Base Plate Integrating Sphere Set, and polarizer assembly separately.)
measurement
Variable incident angle measurement Variable Angle Measurement Unit (Requires large-sample compartment and polarizer assembly separately.)
Normal measurement Integrating Sphere Attachment (ISR-2600, ISR-2600Plus, ISR-603)
Relative diffuse
Requires a large integrating sphere
reflectance Integrating Sphere Attachment, 150 mm Dia. (ISR-1503, ISR-1503F)
(due to ISO compliance and other reasons).
measurement
Large sample size (over 100 mm square) Large-Sample Compartment (MPC-2600A/603A, or SolidSpec-3700)
Normal measurement Integrating Sphere Attachment (ISR-2600, ISR-2600Plus, ISR-603)
Requires a large integrating sphere
Transmittance measurement Integrating Sphere Attachment, 150 mm Dia. (ISR-1503, ISR-1503F)
(due to ISO compliance and other reasons).
Large sample size (over 100 mm square) Large-Sample Compartment (MPC-2600A/603A, or SolidSpec-3700)
Rough
Surface Normal measurement Integrating Sphere Attachment (ISR-2600, ISR-2600Plus, ISR-603)
Relative diffuse
Sample** Requires a large integrating sphere
reflectance Integrating Sphere Attachment, 150 mm Dia. (ISR-1503, ISR-1503F)
Reflectance (due to ISO compliance and other reasons).
measurement
measurement Large sample size (over 100 mm square) Large-Sample Compartment (MPC-2600A/603A, or SolidSpec-3700)
Consult your Shimadzu representative.
Absolute diffuse reflectance measurement
(Depends on the sample. A method using conversion from the mirror reflectance, for instance, is available.)
Large sample size (over 100 mm square) Large-Sample Compartment (MPC-2600A/603A, or SolidSpec-3700)
Small sample size (below 5 mm square) Micro Sample Holder + Micro Beam Lens Unit

* Metals with a mirror-finished surface, mirrors, transparent acrylic and films, etc.
** Paper, cloth, plastics, semi-transparent films, etc.
For color measurement, the Color Analysis Software or LabSolutions UV-Vis Color Measurement Software is required separately. For film thickness measurement, the Film Thickness
Measurement Software is required separately.

Liquid Samples
Samples Measurement Method and Conditions Accessories
Sample volume: 2.5 mL min. Standard Sample Compartment + 10 mm Cell
1 mL min. Semi-Micro Cell + Micro Cell Holder with Mask
500 μL min. Micro Cell + Micro Cell Holder with Mask
Micro-volume measurement
50 μL min. Super-micro Cell + Super-micro Cell Holder
For automatically measuring samples in multiple cells MMC-1600 8/16 Series Micro Multi-Cell Holders and Cells
Samples with high absorbance, but that are difficult to dilute (short optical path measurement) Short-Path Cell (1, 2, 5 mm) + Spacer for Short-Path Cell
Samples with low absorbance, but that are difficult to concentrate (long optical path measurement) Long-Path Cell (20, 30, 50, 100 mm) + Long-Path Rectangular Cell Holder
Normal measurement Multi-Cell Sample Compartment (sample volume: 2.5 mL min.)
For automatically
measuring samples Small sample volumes (50 μL min.) MMC-1600 8/16 Series Micro Multi-Cell Holders and Cells
Transparent in multiple cells Requires temperature control
CPS-100 Six-Cell Thermoelectrically Temperature-Controlled Cell Positioner
Samples (sample volume: 2.5 mL min.)
For temperature- Temperature-controlled with water circulation Constant-Temperature Cell Holder + NTT-2200P Constant-Temperature Water Circulator
controlled
measurements Normal measurement TCC-100 Thermoelectrically Temperature-Controlled Cell Holder
Thermoelectrically
(constant- temperature For automatically measuring samples in multiple cells CPS-100 Six-Cell Thermoelectrically Temperature-Controlled Cell Positioner
temperature controlled
measurement) Tm analysis or variable temperature measurement S-1700 Thermoelectric Single Cell Holder
Requires temperature control (constant-temperature water circulation) 160C Sipper Unit + NTT-2200P
Automatically Temperature control not necessary 160L/160T/160U Sipper Unit (Select type based on liquid volume.)
supplies sample
to flow cells Requires accurate Requires temperature control Syringe Sipper CN + NTT-2200P (Select flow cell based on liquid volume.)
(automatic analysis) control of (constant-temperature water circulation)
aspiration volume. Temperature control not necessary Syringe Sipper N (Select flow cell based on liquid volume)
For automating measurement of multiple samples Sipper Unit or Syringe Sipper + ASC-5 Auto Sample Changer

Absorption measurement Wavelength range: 240 nm min. Integrating Sphere Attachment (ISR-2600, ISR-2600Plus, ISR-603)
of suspension samples For measuring UV region above 190 nm SolidSpec-3700DUV
Suspension
Samples Light transmitted light turbidity measurement 10/50 mm Cell + Long-Path Rectangular Cell Holder
Turbidity measurement (commonly used measurement method) (Optical path length of cell depends on test method.)
Integrating sphere turbidity measurement Integrating Sphere Attachment (ISR-2600, ISR-2600Plus, ISR-603)

4
Basic Measurement

The following accessories are required for measurements using all Shimadzu spectrophotometer models.

Cells
Samples are placed in cells for measurement. The figure below A square cell with a stopper is generally used to measure volatile
shows the ten sample cell shapes. Generally, the rectangular cell liquid samples, and a microcell is used to measure a small-volume
with an optical path length of 10 mm is used. In cases when sample.
absorption is low, a cell with a longer optical path length is Sample cells are made of one of the materials listed below.
used, whereas when absorption is high, a cell with a shorter The wavelength range for each type of material has been
optical path length is used. The relationship between absorption established as follows:
strength (absorbance) and cell length is described below. By Quartz (S cells) : 190 to 2500 nm
utilizing this formula, an appropriate optical path length can be Quartz (IR cells) : 220 to 3200 nm
determined.
Absorbance (A) = ε · C · L
ε : Absorption coefficient
(a constant for given samples)
C : Concentration of sample
L : Optical path length

4 4

12.5
25
45
12 12
ø
25

12
3 2

.5
2 2
12
.5

12.5
Unit: mm

(1) (2) (3) (3)’ (4) (5) (6) (7) (7)’ (8)

Optical Required Sample

Description Type Quartz (S) Quartz (IR)
Path (L) Volume
Note 1)
10 mm 2.5 to 4.0 mL (1) 200-34442 200-66579-01
20 mm 5.0 to 8.0 mL 200-34446 200-66579-02
Square Cell Note 2)
50 mm 12.5 to 20.0 mL (6) 200-34944 200-66579-03
100 mm 25.0 to 40.0 mL 200-34676 200-66579-04
Square Cell with Stopper 10 mm 2.5 to 4.0 mL (2) 200-34444 200-66579-21
Note 3)
Semi-Micro Cell 10 mm 1.0 to 1.6 mL (3) 200-66501 200-66579-11
Note 3)
Semi-Micro Black Cell 10 mm 1.0 to 1.6 mL (3)’ 200-66551 200-66579-12
*1 Note 4)
5 mm 25 to 100 µL (7)’ 208-92116 —
Super Micro Black Cell *2 Note 4)
10 mm 50 to 200 µL (7) 200-66578-11 —
Note 4)
Micro Black Cell 10 mm 50 to 400 µL (8) 200-66578-12 —
10 mm 3.8 mL 200-34448 (silica window) 200-66579-31 (IR silica window)
20 mm 7.6 mL 200-34472 (　〃　) 200-66579-32 (　 〃 　)
Cylindrical Cell (4)
50 mm 19.0 mL 200-34473-01 (　〃　) 200-66579-33 (　 〃 　)
100 mm 38.0 mL 200-34473-02 (　〃　) 200-66579-34 (　 〃 　)
1 mm 0.3 to 0.4 mL 200-34660-01 200-66579-05
Short Path Cell 2 mm 0.5 to 0.8 mL (5) 200-34655 200-66579-06
5 mm 1.3 to 2.0 mL 200-34449 200-66579-07
*1 50 to 100 μL for UV-1280 and UVmini series models.
*2 100 to 200 μL for UV-1280 and UVmini series models.

Note 1: If a cap is required for 10 mm square cells, purchase a cap (P/N 200-34565-02).
Note 2: The 100 mm cell cannot be used in UV-1280 and UVmini series models. The wide 100 mm cell can be used (see UV-1280 brochure.)
Note 3: If used with a 5 nm or wider slit, a Micro Cell Holder with Mask (P/N 204-06896) is required. In UV-1280 or UVmini series models,
a Micro Cell Mask for Six-Cell Holder (P/N 206-66828) is required for measurements using a Multi-Cell Sample Compartment (P/N 206-60605-42),
and a Micro Cell Holder with Mask (P/N 206-66828) is required for measurements using a Sample Compartment Unit (P/N 206-60184-07).
Note 4: Requires a Super-Micro Cell Holder (see page 10.) In UV-1280, UVmini series or MultiSpec-1500 models, a Sample Compartment Unit is also required.

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 5
 Film Holder
(P/N 204-58909)

Holds thin samples, such as films and filters.

• Measurement sample size: Min. W 16 × H 32 mm

Max. W 80 × H 40 × D 20 mm
Note 1: Sample Compartment Unit (P/N 206-60184-07) is necessary for the UV-1280,
UVmini series, MultiSpec-1500.
Note 2: If used in a SolidSpec-3700 Spectrophotometer, then a Direct Detection Unit is
required (see page 27).

Rotating Film Holder

(P/N 206-28500-41)

This film holder enables in-plane rotation of samples centered on the optical
axis. It can be used to attach Polarizer Type I, II, or III.
Large Polarizer Sets cannot be used.

• Measurement sample size: W 33 × H 30 × D 2 mm

Note: For UV-1600/1700/1800/1900 series, it can be used to attach Polarizer Type I and II only.

Didymium Filter
(P/N 202-30242-09)

Holmium Filter
(P/N 202-30242-05)

This can be used for simple operation check. Didymium Filter Holmium Filter

Note 1: Expert evidence, such as wavelength accuracy, is not included. Therefore, it cannot be
used for performance verification and device management in accordance with applicable
laws, regulations and standards.
Note 2: The spectrum shown is one example. The peak wavelength and other characteristics can
vary depending on the lot.

100.0 100.0

80.0 80.0

60.0 60.0
T%

T%

40.0 40.0

20.0 20.0

0.0 0.0
300.0 400.0 600.0 800.0 900.0 300.0 400.0 500.0 600.0 700.0
nm nm

Spectrum Using a Didymium Filter Spectrum Using a Holmium Filter

6
Four-Cell Sample Compartment Unit with Holder
(P/N 206-23670-91)

Accommodates four-cell holders of various types.

• Includes a four-cell holder for 10-mm rectangular cells.
Note: Rectangular cells are not included. Purchase them separately.

Multicell Sample Compartment (Six Cells)

(See page 2 for part numbers)

Holds up to six 10 mm square cells. No temperature control capability.

• Number of cells: Six on the sample side

One on the reference side (No reference side for UV-1280 and
UVmini series.)
Note: Rectangular cells are not included. Purchase them separately.

Sample Compartment Unit

UV-1280, UVmini series, MultiSpec-1500 only (P/N 206-60184-07)

The Sample Compartment Unit is required for using other types of cells (such as a
micro cell, micro flow-thru cell, long-path rectangular cell, cylindrical cell, film holder,
or constant-temperature cell) in UV-1280, UVmini series, or MultiSpec-1500 models.
Remove the standard cell holder and replace it with the sample compartment unit.

Cell Type Sample Holder

(P/N 207-21637-41)

Holds 9 to 10 mm square samples and can be placed in a regular cell holder for
measurements.

Glass/Film Holder for Standard Sample Compartment

(P/N 207-21573-41)

Precisely holds 15 mm square glass samples for measurements.

A polarizer can also be held at the same time, so that polarized light from samples
can be measured. It can also hold 30 to 50 mm square samples.

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 7
 Simplified Near Infrared Light Measurement
The wavelength measurement range can be extended to include the
infrared region by using a photomultiplier tube compatible with near
infrared light. That involves replacing the photomultiplier tube built
in the main unit.

PMT, R5108
UV-2700 only (P/N 206-29869-41)

• Measurement wavelength: 400 to 1,150 nm

• Noise: Approx. within ±2%

Note: Requires replacement by a qualified service engineer.

100% Baseline

Short Optical Path Measurement

100.0
Used to measure samples with absorbance too high to be
measured with a standard 10 mm square cell. 80.0

60.0
%

40.0

20.0

0.0
200 600 1200 1800 2400 3000
nm

Absorption Spectra of Toluene (C6H5CH3) Measured Using a Cell with

Either a 1 or 10 mm Optical Path Length

Spacers for Short-Path Cells

Optical Path Length of Cell
The short-path cell can be used to measure concentrated samples without dilution,
even if the sample is too concentrated to measure using the standard cell with a 10
mm optical path length.
As shown in the figure at right, the spacer is mounted in a standard rectangular cell
holder in conjunction with a short-path cell.

P/N (Spacer) Optical Path Length of Cell

204-21473-03 1 mm
204-21473-01 2 mm
204-21473-02 5 mm

8
Long Optical Path Measurement
2.00
When the concentration of a sample is too low to be measured using a
standard 10 mm optical path length cell, a cell with a longer optical path
1.50
length is used for measurement at the optimal absorption sensitivity. As Optical Path Length: 100 mm

shown in the figure on the right, the absorption spectrum of potassium

Abs.
1.00
permanganate becomes clearer when a 100 mm cell is used instead of a 10
mm cell.
0.50

Optical Path Length: 10 mm

0.00
450.0 500.0 550.0 600.0
nm

Spectrum of Potassium Permanganate

Four-Cell Type Long-Path Rectangular Reference-Side Long-Path Rectangular

Cell Holder Cell Holder
(P/N 204-27208) (P/N 204-28720)

Holds four rectangular cells: 10, 20, 30, 50, 70 and 100 mm in path If using a Four-Cell Type Long-Path Rectangular Cell Holder, use a
length. reference-side cell holder as required.
Note 1: The Four-Cell Sample Compartment (P/N
206-23670-91) is necessary.
Note 2: If the measurement requires a long
rectangular cell at the reference side, a
Reference Long-Path Rectangular Cell
Holder (P/N 204-28720) is necessary.
Note 3: 70 mm and 100 mm cells cannot be used
for UV-1280 and the UVmini series.

Long-Path Rectangular Cell Holder

(P/N 204-23118-01)

Holds two rectangular cells: 10, 20, 30, 50, 70 and 100 mm in path length.
Note 1: A Sample Compartment Unit (P/N 206-60184-07) is required if used in UV-1280, UVmini series, or
MultiSpec-1500 models.
Note 2: 70 mm and 100 mm cells cannot be used in UV-1280 and UVmini series models.
The wide type 100 mm cell and Long-Path Rectangular Cell Holder (for wide cells) can be used.
(See UV-1280 brochure)

Cylindrical Cell Holder

(P/N 204-06216-02)

Holds two cylindrical cells: 10, 20, 50 and 100 mm in path length.
Note: A Sample Compartment Unit (P/N 206-60184-07) is required if used in UV-1280, UVmini series, or
MultiSpec-1500 models.

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 9
 Micro-Volume Measurement
The following accessories are used to measure micro-volume samples. This technique allows a sample size as small as 50 µL to be
measured using a super micro cell. Thus, this technique can be used for biochemical analysis in which minute samples are measured.

Super-Micro Cell Holder

(See page 2 for part numbers.)

Accommodates super-micro cells for measurement of extremely small-volume

samples. Using the adjustable cell height function, the sample volume can be
adjusted between 50 and 200 μL, depending on the type of black cell used.

• Applicable cells: Cells numbered (7), (7)′, and (8) in the list of cells on page 5.
• Mask: W 1.5 × H 1 mm or W 1.5 × H 3 mm selectable
• One cell each can be placed on the sample and reference sides.
Mask

Cell
Optical (P/N 206-14334)
Path Hole
Bottom Sample waste can be minimized due to the adjustable cell height design.
Adjustment Screw
of Cell
Cell height can be optimized by turning the cell height adjustment screw from
below, with the cell installed.
Holder

Note 1: A Sample Compartment Unit (P/N 206-60184-07) is required if used in UV-1280,

UVmini series, or MultiSpec-1500 models.
Note 2: In UV-1280 or UVmini series models, use this holder for sample volumes of 100 μL or
more.
Hexagonal Wrench for Note 3: For UV-1700 models, use P/N 206-55050-91.
Adjusting Height

Micro Cell Holder with Mask

(P/N 204-06896)

Required when using micro and semi-micro cells with an optical path width of 4
mm or less. (The mask width can be continuously adjusted.)
Note 1: If using a semi-micro cell with a 4 mm optical path width in UV-1600/1700/1800/1900
Series models, then samples can be measured using the standard cell holder rather
than this accessory.
Note 2: In UV-1280 and UVmini series models, this is only used for semi-micro cells with an
optical path width of 4 mm. (It is not recommended for cells with a shorter optical
path width.)
Note 3: A Sample Compartment Unit (P/N 206-60184-07) is required if used in UV-1280,
UVmini series, or MultiSpec-1500 models.

10
3 µL Capillary Cell Kit for Ultramicro Volume Measurement
(P/N 206-69746)

This kit is for use with ultra-trace samples, such as a biological sample. Suck the sample into
the capillary and set it to the capillary adapter cell for measurement. It can be set to a standard
cell holder since the size is the same as a 10 mm square cell.

• The minimum amount of sample required: 3 μL (depends on the calculation at the time of
use tube closure)
• Capillary inner diameter: 0.5 mm dia.
• Capillary (quartz) 100, with tube closure
Note: Effective optical path length is typically about 1/20 of 10 mm square cell.

MMC-1600 8/16 Series Micro Multi-Cell Holders and Cells

(P/N 206-23680-58)

MMC-1600C 8/16 Series Constant-Temperature Micro Multi-Cell Holders and Cells

(P/N 206-23690-58)

This cell holder holds one micro multi-cell, either 8 or 16 cell, for micro-volume measurement.
Two micro multi-cell holders are available: a standard type (MMC-1600) and a constant
temperature water circulation type (MMC-1600C).
Note 1: A Constant-Temperature Water Circulator is required when using the MMC-1600C.
Note 2: Choose one of the following micro multi-cells.

Micro Multi-Cells
Standard Sample Volume Optical Path (L) Required Sample
Volume P/N
8 Series Micro Multi-cell 10 mm 100 µL 208-92089 • Micro-volume samples can be measured
16 Series Micro Multi-cell 10 mm 100 µL 208-92088 (Minimum sample volume: 50 or 100 μL)
8 Series Micro Multi-cell 5 mm 50 µL 208-92086 • Support for commercial microplates and micro pipettes (with 8 Series micro cell)
16 Series Micro Multi-cell 5 mm 50 µL 208-92085 • Up to 16 samples can be measured at a time (with 16 Series micro cell)
• Operating temperature range:
There are two types of micro multi-cells available in both the
10 to 60 °C (only for the constant-temperature water circulation type)
8 Series and the 16 Series models: a 50 µL type and a 100 µL
• Temperature difference between constant-temperature water and cell interior:
type. The cell intervals of the 8 Series Micro Multi-cell are
3 °C or less (only for the constant-temperature water circulation type)
applicable for use with 8 × 12 well microplates and 8-channel
• Temperature stabilization time:
pipettes. Microplate samples aspirated into multi-channel
Max. 15 min (only for the constant-temperature water circulation type)
pipettes can be injected directly into the cells for
• Connecting Joint outer diameter: 6 mm and 9 mm (two levels)
measurement.
(only for the constant-temperature water circulation type)

Micro Cell Mask for Standard Six-Cell Holder

UV-1280, UVmini series, MultiSpec-1500 only (P/N 206-66828)

This mask is used to narrow the flux width when 4 mm or narrower micro
cells are placed in the Multicell Sample Compartment for measurement in
UV-1280, UVmini series, or MultiSpec-1500 models.
• Applicable cell:
10 mm Semi-micro cell (quartz) (P/N 200-66501)
10 mm Semi-micro black cell (quartz) (P/N 200-66551)
Note: This cannot be used with a CPS-100 Cell Holder Positioner.

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 11
 Constant-Temperature Measurement
The following accessories are used for biological component measurement, Time-Course Graph
2.0
enzymatic activity measurement and reaction rate analysis for which the
sample temperature must be kept constant.
1.5
The figure shows an example of using a UV-2600 spectrophotometer
combined with a CPS-100 Six-Cell Thermoelectrically Temperature-

Abs.
Controlled Cell Holder Positioner to measure the enzymatic activity of ALP. 1.0

This enzyme is used for enzymatic quantitation of lecithin during reactions

between phospholipase C and choline oxidase. A high ALP value may indicate
0.5
liver damage.

• Substrate : 0.1 mM
• Enzyme : 0 to 0.05 U 0.0
0.0 1000.0 2000.0 3000.0 3600.0
• Temperature : 37 °C Time (Sec.)
• Detection wavelength : 420 nm
Example of Measuring Enzymatic Activity of ALP

Constant-Temperature Cell Holder

(P/N 202-30858-44)

Maintain a sample cell and reference cell at a desired, uniform temperature by

circulating constant-temperature water.

• Temperature range: 5 to 90 °C (depends on the performance of the

constant-temperature water circulator)
• Accepts a pair of 10 mm square cells
• Connection joint outer diameter: 6 mm and 9 mm
Note 1: Sample Compartment Unit (P/N 206-60184-07) is necessary for the UV-1280,
UVmini series, and MultiSpec-1500.
Note 2: Requires a Constant-Temperature Water Circulator.

Constant-Temperature Four-Cell Holder

(P/N 204-27206-02)

Maintain four sample cells and a reference cell at a desired, uniform temperature by
circulating constant-temperature water.
Enables four samples to be placed simultaneously.
• Temperature range: 5 to 90 °C (depends on the performance of the
constant-temperature water circulator)
• Accepts four 10 mm square cells plus a reference cell
(Four cells can be placed on the sample side and one cell on the reference side.)
• Connection joint outer diameter: 9.5 mm
Note 1: The Four-Cell Sample Compartment (P/N 206-23670-91) is necessary.
Note 2: Requires a Constant-Temperature Water Circulator.

12
NTT-2200P Constant-Temperature Water Circulator
(P/N 208-97263)

Circulates temperature-controlled water to a constant-temperature cell holder.

• Temperature range: (Ambient +5) °C to 80 °C

• Temperature control precision: ±0.05 °C
• Max. pumping rate: 27/31 L/min, Max. lifting height 9.5/13 m (50/60 Hz)
• External circulation nozzle: 10.5 mm O.D. (both outlet and return)
• Tank capacity: About 10 L (9 L during use)
• Safety features: Detection of over-temperature of Upper or Lower limits, Detection of heater
wire malfunction, Protection of heating too little circulating water, Detection of sensor
malfunction, Independent over treat protection, Over current circuit protector
• Standard accessories: Lid with handles, Rubber hose (4 m, one tube with 8 mm I.D. and 12
mm O.D.), Hose clamps (4 pcs.), Instruction manual
• Dimensions: W 270 × H 560 × D 400 mm
• Power requirements: 100 VAC, 1250 VA, with 1.7 m power cord and grounded plug
Note 1: NTT-2200P is not sold in Europe since it is not a RoHS compliant product.
Note 2: Not adoptable to S-1700 nor TMSPC-8

CPS-100 Six-Cell Thermoelectrically

Temperature-Controlled Cell Positioner
(P/N 206-29500-XX)

These six-cell positioners permit measurement of up to six sample cells

under constant-temperature conditions.
When used in the kinetics mode, a system can be configured to
measure enzyme activity in up to six samples maintained at a constant
temperature.

• Number of cells: Six on the sample side (temperature controlled)

Note 1: Square cells are not included in the standard contents.
One on the reference side (temperature not controlled) Note 2: A USB adaptor CPS (P/N 206-25234-91) is required when used in
• Temperature range: 16 to 60 °C UV-1280, UV-1800/1900, or UV-2600/2700 models.
• Temperature display accuracy (difference from the true value): ±0.5 °C Note 3: The Kinetics Program Pack (P/N 206-89756-92) cannot be used to
simultaneously measure multiple samples in the UVmini-1240.
• Temperature control precision (variation of temperature): ±0.1 °C
Note 4: The following power supply is required: 100 to 120 or 220 to 240 VAC,
• Ambient temperature: 15 to 35 °C 50/60 Hz, 130 VA

TCC-100 Thermoelectrically
Temperature-Controlled Cell Holder
(P/N 206-29510-XX)

Uses Peltier effect for controlling the sample and reference

temperature, so no thermostated bath or cooling water is required.
• Number of cells: One each on the sample and reference sides
(temperature controlled)
• Temperature range: 7 to 60 °C
• Temperature display accuracy (difference from the true value): ±0.5 °C Note 1: Square cell (P/N 200-34442) not included. Needs to be purchased
• Temperature control precision (variation of temperature): ±0.1 °C separately.
Note 2: Contact your Shimadzu representative if a stirrer is required.
Note 3: The following power supply is required: 100 to 120 or 220 to 240 V AC,
50/60 Hz, 130 VA

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 13
 S-1700 Thermoelectric Single Cell Holder
(P/N 206-23900-XX)

This cell holder permits setting of a temperature program to increase

and decrease the sample cell temperature.

• The thermoelectric system allows prompt control of sample

temperature between 0 °C and 110 °C.
• Temperature increase/decrease speed can be changed using 12
settings, which means the holder can be used in analysis of melting
curves for nucleic acids, etc. that occur during quick as well as slow
heating (or cooling).
• A stirrer is also provided to ensure uniform temperature distribution • Temperature accuracy in cell (when room temperature is 25 °C)
throughout the cell. Within ± 0.25 °C (0 to 25 °C)
• Cooling water circulation is required for Peltier element cooling. Within ± 1% °C of set value (25 to 75 °C)
Though tap water can be used, use of a consistent-temperature Within ± 2% °C of set value (75 to 110 °C)
water circulator is highly recommended. • If the water temperature is high or low, it might be difficult to cool
• Temperature is not controlled at the reference side. or warm to desired temperature. In addition, there is a risk of dew
• Cells are not supplied. Please use 10mm square tight-sealing cells condensation or pipe clogging.
(a Hellma product).
Note 1: Purchase a constant-temperature water circulator that satisfies the
Type Optical Path Length Minimum Sample Volume Required following specifications.
110-QS-10 10 mm 3.5 mL Cooling water temperature: 20 ± 2 °C; Flow rate: 4.8 L/min or more;
Inner diameter of connecting tubing: 4 mm
115B-QS-10 10 mm 400 µL
Note 2: To prevent the condensation on the surface of cell when covering
measurement point under 10 °C, Nitrogen gas (or dry air) supply to purge
connector is required. The equipments below are necessary for purging.
Flow rate: Approx. 3 L/min (less than 5L/min)
Inner diameter of the connecting tubing: 4 mm
Note 3: The following power supply is required: 100 to 120 or 220 to 240 V AC,
50/60 Hz, 110 VA

TMSPC-8 Tm Analysis System

(P/N 206-24350-XX)

Using the Tm Analysis Software, temperature vs. absorbance graph

data is uploaded to a computer to analyze the Tm (melting
temperature) of nucleic acids (DNA and RNA) or other substances. The
Tm Analysis System includes a thermoelectric 8-cell holder, Tm
Analysis Software, and specialized controller. It does not include an 8
series micro cell, silicone cap, or constant-temperature water circulator
for protecting the Peltier element. These must be purchased
separately. Applications
• Predicting the thermal stability and structure of nucleic acids
Model P/N • Analyzing and optimizing hybridization
8 Series Micro Cell, Optical Path: 10 mm, Sample Volume: 100 μL 208-92097-11 • Screening antisense and antigen sequences
8 Series Micro Cell, Optical Path: 1 mm, Sample Volume: 35 μL 208-92140 • Validating targets using antisense and antigen methods
Silicone Cap for Micro Cell (Set of 24) 206-57299-91
Note 1: Purchase a constant-temperature water circulator that satisfies the
• Temperature control range: 0.0 to 110.0 °C following specifications. Cooling water temperature: 20 ±2 °C; Flow
rate: 4.8 L/min or more; Inner diameter of connecting tubing: 4 mm
• Tm calculation mode: Average Method, Differential Method
Note 2: To prevent the condensation on the surface of cell when covering
(Requires a separate computer equipped with an RS-232C port.) measurement point under 10 °C, Nitrogen gas (or dry air) supply to purge
• OS: Windows 10 Pro 64 bit edition, Windows 7 Professional 32/64 connector is required. The equipments below are necessary for purging.
bit edition Flow rate: Approx. 3 L/min (less than 5L/min)
Inner diameter of the connecting pipe: 4 mm
Note 3: The following power supply is required: 100 to 120 or 220 to 240 V AC,
50/60 Hz, 110 VA

14
Automatic Analysis
The following accessories are used to continuously send samples to a sample compartment for measurement.
The sipper unit automatically supplies sample solutions in cells using a step motor-driven peristaltic pump. When this unit and the
auto-sample changer are used simultaneously, up to 100 samples can be measured automatically in approximately 20 minutes. The
micro flow cell can also be used for continuous measurement of the column effluent.

Sipper Units
Four types of sipper units with different cell types are available. The
stepping motor-driven peristaltic pump ensures reliable and smooth
aspiration of sample solution.
(It can be driven directly from the UV-unit so no interface is required.)
Note: The use of a Solenoid Valve (P/N 204-06599-01) and the SWA-2 Sample
Waste Unit (P/N 206-23820-91) are recommended when strong acids,
strong alkalis, or organic solvents are to be measured.

Type and Configuration of Flow Cells

160L (P/N 206-23790-51) 160T (P/N 206-23790-52) 160C (P/N 206-23790-53) 160U (P/N 206-23790-54)
Sample Outlet Sample Outlet Constant- Sample Outlet
Temperature Sample
Sample Outlet Water Outlet Outlet
Luminous Luminous Luminous Luminous
Flux Diaphragm Flux Diaphragm Flux Diaphragm Flux Diaphragm Luminous Luminous
Flux Diaphragm Flux Diaphragm
Reflecting
Mirror

Optical Optical
Optical Path Path
Path Optical
Path

Constant-
Temperature
Inlet
Inlet Sample Water Inlet Inlet
Inlet

Cross-Section of a L-Type Flow Cell Cross-Section of a T-Type Flow Cell Cross-Section of a C-Type Flow Cell Cross-Section of a U-Type Flow Cell
Standard Type Triple-Pass Type Constant-Temperature Type Super-Micro Type
(Required Sample Volume: 2.0 mL) (Required Sample Volume: 1.5 mL) (Required Sample Volume: 25 mL) (Required Sample Volume: 0.5 mL)

• Model 160L uses the standard flow cell, which is L-shaped. • Model 160C uses a constant-temperature type flow cell. Due to the
• Model 160T uses a triple-pass flow cell. Due to the long narrow double-walled structure of the flow cell, constant-temperature
roughly-straight shape of the flow cell, samples flow smoothly into water can be circulated around the flow cell to ensure more
and out of the cell, which minimizes contamination and the accurate and efficient constant-temperature measurements.
formation of bubbles, even with samples prone to bubbles. • Model 160U is for micro volumes. It enables measurement of smaller
quantities, with lower carryover levels, than other flow cells.

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 15
 Syringe Sippers
N (P/N 206-23890-51)
CN (P/N 206-23890-52)
Two types of syringe sippers are available: the normal-temperature
type (N) and the constant-temperature, water-circulating type (CN).
The sipper unit employs a syringe pump system. The liquid contact
surfaces are composed of Teflon, glass, or quartz, imparting excellent
chemical resistance and ease of maintenance, and allowing
measurement of almost any sample type. Further, the extremely high • The ability to replace only the flow cell
repeatability of sipping volume (repeat precision: ±0.03 mL) makes it provides easier maintenance.
ideal when performance validation is required. • Temperature range of circulating water:
Flow cell available separately. Choose from the recommended flow room temperature to 60℃ (CN type)
cells listed below. Note 1: If a rectangular flow cell (micro or super-micro) is used, attaching Mask
R (P/N 206-88679) to the reference cell holder is recommended to
Recommended Flow Cells
balance the light intensity.
Optical Path Dimensions of Standard Required
Cell Type P/N Length Aperture Sample Volume Note 2: The Sample Compartment Unit (P/N 206-60184-07) is necessary for
Square (Ultra-micro) 208-92114 10 mm ø2 mm 0.9 mL UV-1280 and the UVmini series. Square cell (Ultra-micro) cannot be
used for UV-1280 and the UVmini series.
Square (Micro) 208-92113 10 mm ø3 mm 1.0 mL
Note 3: Inner diameter of the constant-temperature water connecting tubing:
Square (Semi-micro) 208-92005 10 mm H11 × W3.5 mm 5.0 mL 4 mm or 12 to 16 mm (CN type only)

ASC-5 Auto Sample Changer

(P/N 206-23810-XX)

If the ASC-5 is combined with a sipper unit or syringe sipper, it is possible to configure an
automated multisample spectrophotometry system for liquid samples.
• It is equipped with an accurate X–Y–Z 3-axis movement mechanism.
• Up to eight sets of parameters, such as rack size and number of test tubes, can be
memorized in the battery back-up protected files.
• Up to 100 test tubes can be placed on the rack.
Note 1: Supports using commercially available test tube stands with a footprint smaller than 220 × 220 mm.
Note 2: A USB adaptor ASC (P/N 206-25235-91) is required when used in UV-1280, UV-1800/1900, or
UV-2600/2700 models.
Note 3: Cannot be used in the SolidSpec-3700/3700DUV.
Note 4: The following power supply is required: 100 to 120 or 200 to 240 V AC, 50/60 Hz, 66 VA
Note 5: ASC-5 is not sold in Europe since it is not a RoHS compliant product.

SWA-2 Sample Waste Unit

(P/N 206-23820-58)

The SWA-2 is a convenient unit for sample suction and disposal that can be used in place of an
aspirator.
• Built-in suction pump
• Size: W 280 × D 300 × H 450 mm (up to upper edge of gauge)
• Power requirements: 100 V AC, 50/60 Hz, 20 VA
Note: Use this unit instead of aspirators at sites where water pressure can vary, due to potential variability in
sample suction volumes.

Solenoid Valve (Fluoropolymer)

(P/N 204-06599-01)

This accessory is required for strong acid, strong alkaline, and ester solutions, due to the lack of chemical resistance in peristaltic pumps used in
sipper units. It also requires an SWA-2 Sample Waste Unit (P/N 206-23820-58).

16
Micro Flow-Through Cell with Holder
Used for the continuous analysis of samples such as the liquids produced by column
chromatography.

• Tubing I.D.: 1 or 2 mm
Note: Sample Compartment Unit (P/N 206-60184-07) is necessary for the UV-1280, UVmini
series, and MultiSpec-1500.

Optical
Description P/N Cell Volume
Path Length
10 mm Micro Flow-Through Cell with Holder 204-06222 10 mm 0.3 mL
5 mm Micro Flow-Through Cell with Holder 204-06222-41 5 mm 0.15 mL

Front Panel with Holes

(P/N 204-27588-03)

Allows the tubes of a flow-through cell, for example, to be connected through the
front panel of the instrument.

Note: Sample Compartment Unit (P/N 206-60184-07) is necessary for the UV-1280, UVmini
series, and MultiSpec-1500.

Flow Cell for Liquid Chromatography

(P/N 206-12852-41)

This flow cell allows UV-VIS detection for high performance liquid chromatography
at variable wavelengths.

• Inner diameter: 1 mm, optical path length: 10 mm, volume: 8 µL

• Sample side: flow cell, reference side: cell holder with mask
• Stainless steel tubing: 1.6 mm O.D. and 0.3 mm I.D.
Note 1: Not applicable to UV-1280, UVmini series, SolidSpec-3700/3700DUV.
Note 2: UV-1900/1800/1700/1600 series, UV-2600/2700: when connecting an integrator, an
analog signal output interface (either for UV-1900/1800 (P/N 206-25223-91), or
UV-1600/1700 (P/N 204-04757), or UV-2600/2700 (P/N 206-25223-91)) is required.

UV Automated System Connection Kits Connection Cable

(See page 2 for part numbers.)

Enables connection to a Gilson GX-271 liquid handler. The liquid handler performs a
variety of pretreatments automatically, including sample dispensing and dilution, and
the addition of reagents. This connection kit interfaces the spectrophotometer and
the liquid handler.

• The connection kit consists of a flow cell unit and connection cable. The liquid
handler is not included.

Note 1: A Sample Compartment Unit (P/N 206-60184-07) is required for use in UV-1280
Flow Cell Unit
and UVmini series models.
Note 2: Cannot be used in the SolidSpec-3700/3700DUV.

GX-271 Liquid Handler

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 17
 Instrument Validation

Low-Pressure Mercury Lamp Unit

UV-2600/2700 only (P/N 206-28300-58)

This unit is used to attach a low-pressure mercury lamp in the light

source chamber in the main unit for confirming wavelength accuracy.
It can be used in conjunction with the validation software included
with the main unit.

Onsite Measurement (Optical Fiber Application Measurement)

Crossflow Cell for Process Monitor System

UV-2600/2700 series, UV-3600/3600 Plus only (P/N 206-53570-13)

Optical path length is continuously variable from 1 mm to 15 mm to allow monitoring of

samples of any concentration.
This cell can be used for applications such as monitoring reaction processes or controlling the
Exterior of Crossflow Cell
concentration of rinse solutions or liquid waste.
It uses a single 600 μm diameter fiber to achieve high throughput. Flow of Sample

General-purpose SMA905 connectors are used to connect optical fibers.

• Measuring wavelength range: 230 to 800 • Pressure resistance: <1.72 MPa (17.6 kg/cm2)
nm (for 2 m fiber length) • Material: 316SS
• Optical path length: 1 to 15 mm (variable) • Pipe connectors: 1/2 inch SMA905
Connector
• Sample temperature: <130 °C • Optical material: synthetic quartz
Structure of Crossflow Cell

Optical Fiber Coupler Configurations

UV-2600/2700, UV-3600/3600 Plus only (P/N 206-54175-41) Description P/N Process Monitor
System

An optical fiber application system is connected to a UV-VIS UV-2600 206-27600-41

spectrophotometer using a high-precision optical fiber coupler. This UV-2700 206-27700-41 Choose among these
optical fiber coupler has been designed for the UV-2600/2700 and UV-3600/3600 Plus 206-23000-91
UV-3600/3600 Plus to guarantee high throughput and stability. Crossflow Cell Note 2 206-53570-13 ✓

Note 1: If fibers are connected, the spectrophotometer basic specifications for Optical Fiber Coupler
206-54175-91 ✓
measuring accuracy, stray light, etc. are out of guarantee. (with two 0.5 mm optical fibers)
Note 2: Crossflow cell is designed to have maximum throughput at room Optical Fiber, 2 m (set of 2) Note 3
206-53875-92
temperature. Optical Fiber, 5 m (set of 2) Note 3 206-53875-93
Crossflow cells are intended for liquids. They are not used for gases. Optional
10m optical fibers (2 pcs. a set) Note 3 206-53875-94
Note 3: Intensity available for transmission decreases according to the fiber length
by approximately the following ratios. 20m optical fibers (2 pcs. a set) Note 3 206-53875-95

Decrease in UV Range Decrease in Visible Range

Fiber Length
(100% at 0.5 m) (100% at 0.5 m)
2m 80% 95%
5m 60% 92%
10 m 36% 90%
20 m 13% 80%

18
Suspension and Opaque Sample Measurement
Measurement of suspension samples is difficult due to the scattering of reflected light by fine particles in the solution. Integrating
spheres are used in this type of analysis. The glass method involves the placement of a scattering board behind the sample, resulting
in the equalization of scattering coefficients of the reference side and sample side. As shown in Figure 1, the integrating sphere
method involves a barium sulfate-coated sphere that draws the scattered light, allowing all of the light to reach the detector. Since
light cannot penetrate opaque samples, it is reflected on the surface of the samples. Figure 2 shows one case in which incoming light
is reflected symmetrically with respect to the normal line (forward reaction), and another case in which the incoming light is
scattered in different directions. The former is referred to as specular reflection and the latter is referred to as diffuse reflection.

Reference Beam

Water M3 Incoming Light Incoming Light

Suspension Reflected Light
M2 Reflected Light
Sample

Ip+id I0

Side-on Type
Photomultiplier

Standard (a) Specular Reflection (b) Diffuse Reflection

Standard White Board
White Board Integrating
Sample Sphere
Beam
M1
Figure 1. Semi-Transparent Sample Measurement Using Figure 2. Specular Reflection and Diffuse Reflection
the Integrating Sphere Method

Integrating Sphere Attachments Reference Beam

With this instrument, a turbid sample is placed in front of the
M3
incoming light window as shown in Figure 1, and the reflectance of M2
an opaque sample is measured by placing it as shown in Figure 3. In
this case, when light is directed at the sample at 0 degrees, diffuse
reflectance is measured, and when light is directed at 8 degrees, total Side-on Type
reflectance (specular and diffuse reflectance) can be measured. Photomultiplier
I0 Idr

Standard Sample
Sample White Board Integrating
Beam Sphere
M1
Figure 3. Opaque Sample Measurement Using the
Integrating Sphere Method
100.00

100.00
4.000

4.000

80.00

80.00
3.000

3.000

60.00

60.00
0.100 (Abs/div.)

Integrating sphere not used

2.00 (%/div.)
2.000

2.000

40.00

40.00
1.000

1.000

Integrating sphere used

20.00

20.00
0.000

0.000

0.00

0.00

400 500 600 700 750 400 500 600 700 750
(nm)
Spectrum of a Chlorophyll Suspension Solution Reflectance Spectrum of Paint

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 19
 Specular Reflectance Attachment (5° Incident Angle)
(P/N 206-14046-58)

This attachment is used to measure the relative reflectance of specular

reflected light, as indicated in Figure 2 (page 19). Relative specular
reflectance is measured to determine reflectance based on the Reference Sample
Sample
intensity ratio of reflected light from a reference sample versus the
θ θ θ θ
measurement sample. In other words, assuming a reflectance of
100% for the reference sample, it is used to determine the reflectance
of a sample relative to the reference sample. The technique of
Reflected Light Incoming Light Reflected Light Incoming Light
specular reflectance measurement is often applied to the evaluation of
(a) When Set at 100% (b) During Measurement
semiconductors and optical materials. Because the properties of
polarized light present almost no problems at a 5° incident angle,
Relative Specular Reflectance Measurement
measurements can be performed easily without polarizers.

• Samples as large as W 100 × H 160 × D 15 mm can be readily

measured with the UV-1280, UVmini series, UV-1600/1700/1800/1900,
UV-2600/2700, Solidspec-3700/3700 DUV, and MultiSpec-1500.
Samples as large as W 140 × H 160 × D 10 mm can be readily
measured with the UV-2400/2500 series and UV-3600/3600 Plus.
Minimum sample size is 7 mm dia (when using 5 mm dia. aperture).
• Sample placement is easy — just set it on the holder with the
measuring surface down.
Note 1: The Sample Compartment Unit (P/N 206-60184-07) is necessary for the
UV-1280, UVmini series, UV-1200 series, and MultiSpec-1500.
Note 2: Direct Detection Unit (see page 27) is necessary for the
SolidSpec-3700/3700DUV.

Integrating Sphere Attachment List

ISR-2600 ISR-2600Plus MPC-2600A ISR-3100A ISR-603 MPC-603A ISR-1503 ISR-1503F
P/N 206-28400-58 206-28410-58 207-23520-41 206-23851-92 207-20100-58 207-23550-41 207-20900-58 207-21300-58
Spectrophotometer models supported UV-2600/2700 UV-2600 UV-2600/2700 UV-3600 UV-3600 Plus
Inside diameter of integrating sphere 60 mm dia. 150 mm dia.
Aperture During transmittance measurement 5.6% 7.4% 7.7% 6.1% 7.2% 2.9%
ratio During reflectance measurement 7.5% 9.3% 10% 8.4% 9.7% 4.0%
Integrating sphere interior wall material BaSO4 Spectralon
Incident Sample light 0 deg. 8 deg.
light angle Reference light 8 deg. 0 deg.
Detector PMT
PMT PMT
PMT InGaAs
InGaAs PbS
PbS
Wavelength range 220 to 850 nm 220 to 1400 nm 220 to 2600 nm 200 to 2500 nm
Max. sample size for Sample light W95 × H135 × T20 204 dia. × T50 W100 × H100 × T15 W70 × H70 × T20 204 dia. × T50 W235 × H260 × T10
reflectance measurements Reference light W70 × H70 × T12 305 dia. × T5 W100 × H100 × T60 W70 × H70 × T12 305 dia. × T5 W150 × H165 × T10 W150 × H165 × T5
Max. sample size for Sample light W50 × H60 × T5 305 dia. × T300* W100 × H100 × T15 W50 × H60 × T5 305 dia. × T300* —
transmittance measurements Reference light W70 × H60 × T5 W50 × H60 × T15 W50 × H50 × T15 W70 × H60 × T5 W50 × H60 × T15 W176 × H168 × T20
Switching between sample/reference light Yes
Total, diffuse, or specular reflectance measurement Yes
Transmittance measurement Yes
Standard Accessories
Standard white plate (BaSO4) Yes
Cell holder with 10 mm optical path length Yes No
Film holder Yes Sold separately
Powder sample reflectance measurement Yes (Trace quantity model sold separately) Sold separately
Beam aperture mask for sample light Yes (2 × 3 mm transmittance, Yes (1 mm dia. Yes (1 mm dia.
No No
3 × 3 mm reflectance) sold separately) sold separately)
Accessories Sold Separately
Small sample holder Yes No Yes Yes (Transmittance only)
Small beam aperture unit No Yes No Yes No
Large sample holder No Yes (110 mm dia.) No Yes (110 mm dia.) Yes
Manual V-stage No Yes No Yes No
*: use V-stage (sold separately)

20
ISR-2600 Integrating Sphere Attachment UV-2600/2700 only (P/N 206-28400-58)
ISR-2600Plus Integrating Sphere Attachment UV-2600 only (P/N 206-28410-58)
In combination with a 0° or 8° incident angle integrating sphere and S/R exchange functionality
of the spectrophotometer, either diffuse or specular reflectance can be measured without
using any special attachments. The ability to change the size of the flux in the reflectance
measurement unit enables measuring reflectance at micro areas of samples (minimum flux size
of about 2 × 3 mm). The flux size can also be narrowed to about 3 × 3 mm in the
transmittance measurement unit.
The ISR-2600Plus Integrating Sphere Attachment includes two detectors: a photomultiplier
tube and InGaAs detector.
ISR-2600/2600Plus Common Specifications
• Integrating sphere I.D.: 60 mm
• Maximum size of reflectance sample: W 70 × H 70 × D 20 mm (0° incident angle)
W 70 × H 70 × D 12 mm (8° incident angle)
M3 Mask
ISR-2600 Specifications
Reference Beam
• Wavelength range: 220 to 850 nm
• Noise level: 0.1%T RMS 500 nm (UV-2600), 0.3%T RMS 500 nm (UV-2700)
•100% flatness: ± 0.5%T (UV-2600), ± 1.5%T (UV-2700) M2

ISR-2600Plus Specifications 8°
90°
• Wavelength range: 220 to 1400 nm
• Noise level: 0.1%T RMS 500 nm, 0.3%T RMS 900 nm Mask

• 100% flatness: ± 0.5%T (220 to 1300 nm) 60 mm dia. Sample Beam

Integrating Sphere
• NIR stray light: 0.4%T (typical value given 1400 nm, H2O, and 5 nm slit)
M1

ISR-240A Integrating Sphere Attachment UV-3600 only (P/N 206-23860-91)

• Wavelength range: 240 to 800 nm. Reference Cell for Transmission
• Integrating sphere I.D.: 60 mm Measurement
• Detector: Photomultiplier tube Sample Cell for Transmission
Measurement
• Maximum size of reflection sample: W 40 × H 70 × D 10 mm or
W70 × H 70 × D 5 mm.
Reference Beam
• Incident angle: 0 deg
• Two BaSO4 white plates, two powdered sample holders and BaSO4
powder (500 g) are included.
Sample
Note: ISR-240A is not sold in Europe since it is not a RoHS compliant product. BaSO4 White Plate

Detector
(Mounted Sample Beam
at the Bottom)

Optical Diagram

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 21
 ISR-3100A Integrating Sphere Attachment UV-3600 only (P/N 206-23851-92)
ISR-603 Integrating Sphere Attachment UV-3600 Plus only (P/N 207-20100-58)
• Wavelength range: 220 to 2600 nm
• Integrating sphere I.D.: 60 mm
• Detectors: PMT, PbS (ISR-3100A) PMT, InGaAs, PbS (ISR-603)
• Reflectance sample size: Approx. 100 mm dia. × 15 mmt
• Incident light angle: 0°/8°
• Transmittance sample cell holder: Includes a holder for 10 mm rectangular cell (does not
include cells), two BaSO4 standard white plates, and two plates for powdered samples.
Note: ISR-3100A is not sold in Europe since it is not a RoHS compliant product. ISR-3100A ISR-603

150 mm Dia. Integrating Sphere Attachments

ISR-1503 (BaSO4) UV-3600 Plus only (P/N 207-20900-58)
ISR-1503F (PTFE) UV-3600 Plus only (P/N 207-21300-58)
Used to measure the reflectance spectra of solid samples, such as
powders, paper, or fabrics, or the transmittance spectra of liquid or solid
samples. It enables reliable measurements unaffected by factors such as
the sample surface status. Also, low-noise near infrared region measure- • Sample placement orientation: Lateral for
ments can be obtained by using a Spectralon ISR-1503F attachment. transmittance and 0° reflectance, Vertical for 8° reflectance
• Wavelength range: 200 to 2500 nm • Incident light angle: 0° or 8°
• Integrating sphere I.D.: 150 mm • Opening rate: 4.0% (0° reflectance), 4.0% (8° reflectance), or 2.9%
• Detectors: PMT, InGaAs, PbS (transmittance)

Accessories for ISR-1503/1503F 150 mm Dia. Integrating Sphere Attachments

Transmittance Small Sample Holder Film Holder
ISR-1503 only (P/N 207-21742-41) ISR-1503 only (P/N 207-21743-41)
ISR-1503F only (P/N 207-21742-42) ISR-1503F only (P/N 207-21743-42)
This small sample holder is used for 0° This film holder is used for 0° transmittance measurement.
transmittance measurement. It can secure film samples up to 50 × 50 mm.
Flux size: 4 mm diameter
Sample size: 20 mm min Hanging Sample Holder
ISR-1503 only (P/N 207-21750-41)
Cuvette Cell Holder for Direct Detection ISR-1503F only (P/N 207-21750-42)
(P/N 207-21741-41) Used to measure the absorption rate of samples hung inside the
This replaces the included optical system for integrating sphere.
measuring samples in 10 mm rectangular cells. Standard White Plate
Rear Cover with Window This standard white plate is used to measure reflectance. They are
available made of either BaSO4 or Spectralon.
(P/N 207-21858-41)
Material P/N
Used in combination with a Cuvette Cell Holder for Direct Detection. If
207-21744-41 For ISR-1503
installed in place of the normal ISR-1503/1503F rear cover, it enables BaSO4
207-21744-44 For ISR-1503F
easy exchange of samples by simply opening a window.
207-21744-43 For ISR-1503
Spectralon
Powdered Sample Holder 207-21744-42 For ISR-1503F

ISR-1503 only (P/N 207-21815-41)

ISR-1503F only (P/N 207-21815-42)
Sample Holder
ISR-1503 only (P/N 207-21868-41)
This holder is for measuring the reflectance of
ISR-1503F only (P/N 207-21868-42)
compressed powdered samples.
It is installed in the position for measuring 0° or Though samples up to 100 g can be secured for 8° reflectance
8° reflectance. measurements, this holder is used to hold samples with a low coefficient
of friction more securely.

22
150 mm Dia. Integrating Sphere Attachments
LISR-2100 UV-3600 only (P/N 206-23862-91)
LISR-3100 UV-3600 only (P/N 206-23862-92)
This attachment is used to measure the reflectance spectrum of solid
samples, such as powder, paper or cloth.
It is also used to measure the transmittance of solid or liquid samples.Note 1 Reference
Beam
It is suitable for color analysis because stable measurement can be
performed regardless of the surface configuration of the samples.
Sample
Beam
• Measurement wavelength range: 240 to 840 nm (LISR-2100),
240 to 2400 nm (LISR-3100)
PMT
• Integrating sphere I.D.: 150 mm
PbS
• Reflectance sample installation space: Approx. W 150 × H 170 × D 30 mm
(LISR-3100 only)
Note 1: Transmittance measurement accessory (P/N 206-17270) is required.
Note 2: LISR-2100 and LISR-3100 are not sold in Europe since they are not RoHS
compliant products.

Multipurpose Large-Sample Compartments

MPC-2600A UV-2600/2700 only (P/N 207-23520-41)
MPC-3100 UV-3600 only (P/N 206-23831-91)
MPC-603A UV-3600 Plus only (207-23550-41)
These multipurpose large sample compartments can be used freely to measure
the transmittance or reflectance of variously shaped samples. A built-in
MPC-603A
integrating sphere ensures that solid samples can be measured accurately.
Due to the ample sample space around the integrating sphere, they can also be
used for extra-large samples. Included Mirror
M1 R Beam
for Introducing
• Wavelength range: 220 to 1400 nm (MPC-2600A) Light Beam
M5
220 to 2600 nm (MPC-3100) Multipurpose Large-Sample S Beam
Compartment Main Body
220 to 2600 nm (MPC-603A) M4
• Maximum sample size:
M3
305 mm dia. × 50 mmt or 204 mm dia. × 300 mmt for transmittance samples
305 mm dia. × 50 mmt for reflectance samples M2

• The S/R exchange function enables reflectance measurements at zero or 8°

incident angle without tilting the sample.
• The integrating sphere shift function further extends the applicable range.
M6
• Sample positions can be adjusted back and forth or up and down with the
Integrating
use of V-stage. Sphere
V Stage
V-stage for MPC-2600A and MPC-603A need to be purchased separately.
• MPC-2600A and MPC-603A models can be changed easily to a variable Optical System for Multipurpose Large-Sample Compartment
angle attachment or other detector.
Note: MPC-3100 is not sold in Europe since it is not a RoHS compliant product.

Powdered Sample Holder (for Integrating Sphere)

(P/N 206-89065-41)

Powdered sample holder for installation in an integrating sphere.

Can be installed in all integrating spheres.

• Minimum capacity is 0.16 mL.

• 3 holders are included.

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 23
 Micro Beam Lens Unit
(P/N 206-22051-41)

This unit uses a lens and mask to mask the beam that passes through the
integrating sphere opening. The minimum beam diameter is about 1 mm.
It requires a sample base plate integrating sphere unit at the same time.

Integrating
Sphere Lens Unit (shaded area)

for MPC)
ø8 (Uses mask
Approx. 32
2

25

Base

Overview Diagram

Note: For UV-2600, MPC-2600A and BIS-3100 sample base plate integrating sphere set are separately required.
For UV-3600Plus, MPC-603A and BIS-603 sample base plate integrating sphere set are separately required.
For SolidSpec-3700/3700DUV, BIS-3700/3700DUV is separately required.

Micro Sample Holder

(P/N 206-28055-41)

This holds solid samples about 5 to 10 mm square or in diameter and

about 1 to 5 mm thick. Samples are held by clamping from above and
Thumbscrew
below.

Mounted to the Integrating Sphere

Cylindrical Sample Holder

Holds round glass samples for measurements using an integrating sphere.
It can be used with MPC series or SolidSpec models.
5 to 25 mm dia. (P/N 207-23559-41)
30 to 50 mm dia. (P/N 207-23559-42)
40 to 110 mm dia. (P/N 207-23559-43)

24
Absolute Specular Reflectance Attachments
To Detector Incident Light
These attachments are used to measure the absolute reflectance of specular
reflected light, as indicated in Figure 2 (page 19). Reference samples are not used for To Detector Incident Light

absolute specular reflectance measurements as shown in the figure on right. By θ θ

assuming 100% reflectance when no sample (air) is placed in the compartment, light
Sample
reflected from the sample is measured. The attachment is installed in a Large-Sample
Compartment Unit to measure the absolute specular reflectance of mirrors or other (a) When Set to 100% (b) During Measurements
solid samples. It also requires a Sample Base Plate Integrating Sphere Set separately.
Absolute Specular Reflectance Measurement
When the incident angle is large (12°, 30° or 45°), a separately sold polarizer unit is
required due to polarized light effects to ensure accurate measurements.

• V-N method enables switching between light paths for sample measurements and Ref

for 100% setting with a single step. 100% Light M2

• Sample size: Approx. 25 mm dia. or 20 mm square up to 200 mm dia. or 150 mm Measurement Light

square, and up to 30 mm thick

M2’ Polarizer Position
Wavelength Range M3
P/N Incident M1
Model
Angle MPC-2600 MPC-3100/MPC-603A
ASR-3105 206-16817-58 5° 300 to 2400 nm
Integrating Sphere
ASR-3112 206-16100 12° 300 to 2500 nm
300 to 800 nm
ASR-3130 206-15001-58 30° Sample
300 to 2300 nm
ASR-3145 206-15002-58 45°
Structure of Absolute Specular Reflectance Attachment

Sample Base Plate Integrating Sphere Sets

These sets include a sample base plate paired with an integrating sphere. The
accessory is required for attaching absolute specular reflectance attachments to the
main UV spectrophotometer unit.

2600 3600 SolidSpec SolidSpec Wavelength

Model P/N 3600
2700 Plus -3700 -3700DUV Range
BIS-3100 206-17059-58 ✓ ✓ ✗ ✗ ✗
BIS-603 207-21100-58 ✗ ✗ ✓ ✗ ✗ 240 to 2600 nm
BIS-3700 206-20880-51 ✗ ✗ ✗ ✓ ✗
BIS-3700DUV 206-20880-52 ✗ ✗ ✗ ✗ ✓ 175 to 2600 nm

Large Polarizer Set

Polarizer Type I, II, III
Polarizer Adaptor Set
These attachments are used to perform accurate measurements without polarized
light effects using the Absolute Specular Reflectance Attachments. Using Polarizer
Type I, II, or III also requires a Polarizer Adapter Set (P/N 206-15693).

Note: These polarizers are only used in Multipurpose Large Sample Compartments or Absolute
Specular Reflectance Attachments.

Effective Wavelength
Description P/N
Diameter Range
Large Polarizer Set 206-15694-40 20 mm 250 to 2300 nm
Polarizer Type I 206-13236-41 18 mm 400 to 800 nm
Polarizer Type II 206-13236-42 17 mm 260 to 700 nm
Polarizer Type III 206-13163-40 10 mm 260 to 2300 nm

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 25
 Variable Angle Measurement Unit
For MPC-2600A (P/N 207-23490-41)
For MPC-603A (P/N 207-23490-42)

This allows measuring transmittance or absolute reflectance of solid

samples at any angle of incidence or any angle of receiving light.
When the incident angle is larger than 10°, a separately sold polarizer
unit is required due to polarized light effects to ensure accurate
measurements.

• Sample size: 20 to 100 nm, thickness 15 mm max.

• Incident angle: 5 to 70 deg. (movable range: 0 to 90 deg.)
• Angle of receiving light: 10 to 140 deg. (movable range: 0 to 180 deg.)
• Photometric accuracy: ±3％ (MPC-2600A)
±1.5％ (MPC-603A)
• Wavelength range: 250 to 1400 nm (MPC-2600A)
250 to 1650 nm (MPC-603A)

Sample
Reference beam
Mask

60° s

45° s
Integrating Sphere Sample beam
for Reference
20° s

5°
Angles for the Sample holder
and the Detector can be set
20° p
independently. Integrating Sphere
for Sample
45° p

60° p
Movable portion

Overview Diagram Absolute Reflectance from Silicon Wafer Mirror Surface

at 5, 20, 45, and 60 Degree Angle of Incidence

Variable Angle Measurement Unit

26
Special Accessories for SolidSpec-3700

Automatic X–Y Stage

(P/N 206-20810-58)

The Automatic X–Y Stage enables automatic measurements for the points specified in advance
and is a powerful accessory for high-throughput measurements.

• Maximum sample size: 310 mm diameter or 310 × 310 mm, 40 mm thickness

Direct Detection Units

DDU SolidSpec-3700 only (P/N 206-20264-51)
DDU-DUV SolidSpec-3700 DUV only (P/N 206-20264-52)
The same sample compartment as a conventional UV-VIS spectrophotometer can be added to
the SolidSpec-3700/3700DUV by mounting the Direct Detection Unit DDU or DDU-DUV.

• Measurement wavelength range:

DDU: 190 to 3300 nm (when mounted in SolidSpec-3700)
DDU-DUV: 165 to 3300 nm (when mounted in SolidSpec-3700DUV)

Purge Box
SolidSpec-3700 DUV only (P/N 206-21788-58)

The Purge Box is used with the DDU-DUV Direct Detection Unit, allowing the inside to be
purged. The Purge Box has a film holder and a six-cell holder and allows the cell positions to be
moved without opening the cover of the SolidSpec-3700DUV.
• Maximum sample size: 60 × 60 mm, 20 mm thickness

Large Specular Reflectance Attachment (5° Incident Angle)

(P/N 206-20570-58)

The Large Specular Reflectance Attachment is needed for relative specular reflectance
measurements. This accessory is mounted in the main body of SolidSpec-3700/3700DUV and
enables reflectance measurements while keeping the samples horizontal. The Direct Detection
Unit DDU or DDU-DUV is not required for this accessory.

• Applicable sample size: maximum W 470 × D 560 × H 40 mm

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 27
 Variable Angle Measurement Unit
For 100V (P/N 207-23470-41)
For 230V (P/N 207-23470-42)

This allows measuring transmittance or absolute reflectance of solid samples at any

angle of incidence or any angle of receiving light.
When the incident angle is larger than 10°, a separately sold polarizer unit is
required due to polarized light effects to ensure accurate measurements.

• Sample size: 20 to 100 nm, max. 15 mm thickness.

• Incident angle: 5 to 70 deg. (movable range: 0 to 90 deg.)
• Angle of receiving light: 10 to 140 deg. (movable range: 0 to 90 deg.)
• Photometric accuracy: ±1.5%
• Wavelength range: 250 to 2500 nm

Square Cell Holder for Integrating Sphere

(P/N 206-22339-92)

10 mm square cell holder for integrating sphere built into SolidSpec-3700/3700DUV systems.

Printer, Interface Cable

DPU-414 Screen Copy Printer

UVmini Series, UV-1700 only (P/N 206-55215-XX)
UV-1601 only (P/N 206-55210-XX)

DPU-S445 Screen Copy Printer

UV-1280, UV-1800/1900 only (P/N 207-23484-48) DPU-414
Prints hard copies of screens, including numeric data. A printout is made after
each measurement.
Spectra, kinetics reaction data, and quantitation calibration curves displayed on the
DPU-S445
screen are output in the screen print. A hard copy can be printed at any time,
making it simple to record measurement parameters. The printer cable is included. AC Power Cable for DPU-S445

• Size: W 160 × D 170 × H 66.5 mm for DPU-414 Description P/N Country/Region

W 145 × D 135 × H 58 mm for DPU-S445 Cable CB-US04-18A-E 088-52083-36 U.S., Canada

• Thermal paper (10 rolls) (P/N 088-58907-04) Cable CB-CE01-18B-E 088-52083-38 EU, EFTA
• Power supply: 100 to 120 or 200 to 240 V AC, 50/60 Hz, 25 VA Cable CB-UK01-20A-E 088-52083-51 UK
Cable CB-CH01-20A-E 088-52083-52 China
Note: DPU-414 is not sold in Europe since it is not a RoHS compliant product.

Analog Signal Output Interface USB Interface Cable

UV-1280, UV-1800/1900, UV-2600/2700 only (P/N 206-25233-91) UV-1280, UV-1800/1900 only (P/N 088-50602-49)

This interface is used when using an analog data recorder to Used to connect the spectrophotometer to the PC.
record the measurement signal from a spectrophotometer. It
provides analog signal output, such as for monitoring liquid
chromatography, and can be connected to an integrator.
• Analog output full scale: 100 mV/2 Abs. or 100 mV/100%T

28
Optional Software

Water Analysis Program

UV-1280 only (P/N 207-22430-42 English, 207-22430-43 Chinese)

Easy and accurate water analysis can be conducted in combination with simplified
reagents.
• All measurement parameter settings are included internally for 22 sample types / 39 items
for the UV-1280. All items, including measurement wavelengths, calibration curve,
measurement time, and measurement concentration range, are set automatically by
simply selecting the item.
• Results can be acquired even without analytical knowledge through operation in
accordance with screen instructions. The pack comes with an analysis guide that displays
the model number of the reagent to be used and the operation procedure, so there is no
need to refer to the manual.
• If the optional multicell holder (6 cells) is used, up to six cells can be measured
consecutively in one analysis.
• Automatic analysis commences after a specified time. The elapsed time is displayed on
screen, concentration values are displayed automatically after the specified time has Operation Screen
elapsed, and a buzzer sounds to state that analysis is complete.

Note 1: Except some of the items

Note 2: Conjunction measurement with the shipper unit is not possible.

List of Measurable Items

Symbol Measurable item
ClO Residual chlorine (Free)
CN Free Cyanogen
Total Cyanogen
COD COD (chemical oxygen demand)
Color Color
Cr Hexavalent chromium
Hexavalent chromium + 50 mm cell
Measuring Screen
Total Chromium
Cu Copper
F Fluorine (Free)
Fe Bivalent iron
Bivalent iron at low concentration
FOR Formaldehyde
H2O2 Hydrogen peroxide
Mn Manganese
NH4 Ammonium
Ammonium-nitrogen
Ni Nickel
NO2 Nitrite
Nitrite-nitrogen
NO3 Nitrate (No NO2 mixed)
Nitrate (NO2 0.005 mg/L or less)
Nitrate (NO2 5.0 mg/L or less)
Nitrate-nitrogen (No NO2·N mixed)
Nitrate-nitrogen (NO2·N 0.0015 mg/L or less)
Nitrate-nitrogen (NO2·N 1.5 mg/L or less)
Pb Lead (SPK)
Phenol Phenol
PO4 Phosphate
Phosphate (enzyme method)
Phosphate phosphorus
For information about reagents, contact:
Phosphate phosphorus (enzyme method)
Kyoritsu Chemical-Check Lab., Corp.
S Sulfide (Hydrogen sulfide)
(Manufacturer and Distributor)
TH Total hardness 5-37-11, Den-enchofu, Ota-ku,
Turbid. Turbidity (Formazin) Tokyo, 145-0071, Japan
Turbidity (Polystyrene) Tel: +81-3-3721-9207 Fax: +81-3-3721-0666
Zn Zinc.: Not including other metals https://kyoritsu-lab.co.jp/english/

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 29
 LabSolutions Connection Kits
LabSolutions DB Connection Kit (P/N 207-21250-92 English, 207-21250-93 Chinese)
LabSolutions CS Connection Kit (P/N 207-21251-92 English, 207-21251-93 Chinese)

This software is used to perform operations such as measuring data using

UVProbe, automatically registering processed data and PDF report files in a
LabSolutions database, securely managing data, or applying electronic signatures.
Using these kits allows UVProbe to be compliant with FDA 21 CFR Part 11.
They also provide network capability, which allows using a server computer to
centrally manage data from other analytical instruments, such as HPLC, GC, or
FTIR systems, by installing software that is compatible with such instruments.
Viewing the data from a client computer on the network is also made possible.
• Access Control and User Management
FDA 21 CFR Part 11-compliant access control can be achieved by using a user authoriza-
tion server to centrally manage which users can access the program, regardless of the
operating system, in the same manner as with UVProbe. To prevent those without proper LabSolutions Data Manager
access rights from accidentally changing settings, access restrictions for specific
functionality can be specified for each registered user with access permission.
• Security and Audit Trails
Even if electronic records are changed, data before the changes are not lost because all
electronic records are saved and managed in a database. In addition, a record of system
usage, a history of revisions to data recorded in the database, and so on are recorded
automatically together with the date and person that performed each process.
• Data Integrity and Electronic Signatures
Data is automatically saved in the database and cannot be deleted. Data saved in the
database can be easily restored, displayed, or reanalyzed. Electronically recorded data can
be signed with an electronic signature and linked to analytical data, so that the signer's
name, signature date, and reason for signature are saved together with the data.
• Managing Related Information for Each Project
LabSolutions includes a project management function that allows managing information
based on the type of process or system used. This function makes it possible to specify
different settings for each project concerning instrument management, user manage- PDF Report of Data
ment, security policy, and data processing. This helps ensure that searching data and
other management processes can be performed smoothly.
• OS: Windows 10 Pro 64 bit edition, Windows 7 Professional 32/64 bit edition

Tm Analysis Software
(P/N 206-57476-91)

This software is used with the S-1700 cell holder. It enables temperature versus
absorbance graph data to be uploaded to a computer for analyzing the Tm (melting
temperature) of nucleic acids (DNA and RNA) or other substances.

• OS: Windows 10 Pro 64 bit edition, Windows 7 Professional 32/64 bit edition

Note 1: A separate RS-232C cable (P/N 208-94860) is required for connecting a computer to the S-1700 cell
holder.
Note 2: If used for the UV-1800/1900 spectrophotometer, a separate USB cable (P/N 088-50602-49) is
required for connecting the computer to the main unit.

30
Film Thickness Measurement Software
(P/N 206-66877)

This software measures the thickness of thin films from the wavelengths of peak
(or valley) interference waveforms overlapping the spectrum.
The film thickness is measured through optical methods without physical contact.
• The film thickness is calculated from linear regression by applying the method of least
squares to the wavelengths of the multiple peaks and valleys automatically detected. (The
thin film's refractive index and the angle of incidence must be configured as calculation
conditions.)
• The calculation conditions can be changed with respect to the measured spectra, enabling
recalculation.
• A range can be set for use in the calculations while checking the spectral interference
waveform onscreen.
Recalculation Window
• The measurable film thickness range is (minimum measured wavelength)/(film's refractive
index) to 50 × (maximum measured wavelength)/(film's refractive index). (Reference value) Note: If used for a UV-1601 spectrophotometer, an RS-232C interface
• OS: Windows 10 Pro 64 bit edition, Windows 7 Professional 32/64 bit edition cable (P/N 208-94860) is required.

Color Measurement Software

For UVProbe (P/N 206-65207)
For LabSolutions UV-Vis (P/N 207-24528-91)

• Available Calculation Values

Tristimulus values (X, Y, Z), chromaticity coordinates (x, y), CIELAB scale/color difference
formula, yellowness index/yellowing factor, whiteness, Munsell, metamerism, CIELAB-
based three attributes and their difference, primary wavelength, excitation purity, etc.
• Extensive convenient graphical functionality, such as chromaticity diagram and color
difference diagram functions.
• Freely selectable field of view (2° or 10°) and Illuminant (JIS regulations <for UVProbe and
LabSolutions UV-Vis> and ASTM regulations <for LabSolutions UV-Vis>).
• Correction can be calculated by specifying standard white plate values.
• Color differentials can be calculated for any specified reference sample. (For UVProbe)
• Mean and standard deviation values can be calculated for multiple sets of data. Lab Chromaticity Diagram Display Window

(For LabSolutions UV-Vis)

XYZ Chromaticity Diagram Display Window

Solar Transmittance Measurement Software

(P/N 206-23130-92)

This software is used to calculate the solar transmittance, solar • User-settable weighting factor charts can be saved as a file.
reflectance, visible light transmittance, and visible light reflectance. It • Results can be recalculated based on different standard samples.
• Users can create user-defined Illuminant for color value calculations.
also can calculate some color-related values (tristimulus values,
User-specified illumination settings can be saved as a file.
chromaticity coordinates, primary wavelength, and excitation purity). • Highly accurate calculations can be performed using white tile correction.
Reflectance data from the white tile can be saved as a file.
• Chromaticity coordinates can be graphed.
• OS: Windows 10 Pro 64 bit edition, Windows 7 Professional 32/64 bit edition

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 31
 LabSolutions UV-Vis
(P/N 207-24525-92)

LabSolutions UV-Vis is a next-generation Shimadzu UV control software pursuing efficiency

of analysis. The simple design layout enables even first-time users to perform operations
easily. A new spectrum evaluation function automates the measurement, analysis, and
printing of reports to significantly enhance the efficiency of routine analysis. In addition, it
achieves the easy transfer of measurement data. Users can export the measurement data in
text format and import it into other software for analysis with Excel®.
Note: LabSolutions UV-Vis is the latest optional software for UV-1800/1900/2600/2700/3600Plus/
SolidSpec-3700. Each main unit is equipped with UVProbe software as standard.

Simple Design
From the start, the software's user-friendliness allows users to perform operations with ease.
With extensive features, LabSolutions UV-Vis meets a wide range of users' expectations.

Instrument Control Panel

The instrument control panel that brings together the measurement
functions enables automatic measurement, analysis and reporting.

Simple Main Window Easy-to-follow Configuration Window

Clear and simple layout of the graphs and measurement results makes it easy to read. Large icons make it easy for users to understand and operate.

Four Measurement Modes

It permits four measurement modes: spectrum, quantitative, photometric, and time course.
Users can open multiple measurement modes at the same time, so that data analysis can be performed in one mode while collecting data in
another mode.

Report
Easily create report layouts.

Print from the measurement window with one click. Report is printed.

32
Spectra Evaluation Function
In addition to providing measurement and analysis results, judgment results are also provided.
With this feature, LabSolutions UV-Vis enables users to maintain a product's quality.

Quality of the sample can be determined with Multiple evaluation

Detail Settings Window
comprehensive judgment at a glance. criteria can be set.
Evaluation method can be selected from a wealth of choices.

Easy Transfer of Measurement Data

Users want to export measurement data immediately in text format, and import for analysis in other software, such as Excel.

To Analysis Software
Automatically generates a text
file when the spectra data are
saved. It can be immediately
imported into other software.

To Excel
Real-time transfer of the spectrum waveform to Excel during
Matrix Output measurement.
Outputs multiple spectra to one text file. No need to create a CSV file each time.
Easy to import data into multivariate analysis software.

Line up wavelengths vertically.

Line up wavelengths
horizontally.

Select how the data is ordered.

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 33
 Shimadzu offers LabSolutions DB UV-Vis and LabSolutions CS UV-Vis* to meet the requirements of ER/ES regulations.

LabSolutions DB UV-Vis
(P/N 207-24526-92)

LabSolutions DB UV-Vis System can be established by introducing the

database system. The system allows for data management and user
management with a database. Compliant with ER/ES regulations, the
system is optimally configured for customers using a PC.

LabSolutions CS UV-Vis * Client PC Laboratory or office LabSolutions Server

UV-Vis can be added to LabSolutions CS as an acquisition controller.

The system is optimally configured for customers who want to
manage data on a server together with LC and GC data for ER/ES
compliance.
Acquisition controller PC

* Coming Soon

Name LabSolutions UV-Vis LabSolutions DB UV-Vis System LabSolutions CS UV-Vis System

Measured data files are saved and

Data management method Measured data files are saved and managed in the LabSolutions database.
managed in folders on the PC.

The software references files

Data references The software references files in the database.
on drives or in folders on the PC.

Available Available
LabSolutions database Unavailable
(The database resides on a local PC) (The database resides on a server)

User administration Unavailable Available

Rights group administration Unavailable Available
Project administration Unavailable Available

Only databases on the network can be used.

LabSolutions UV-Vis data can be viewed using
Only the standalone configuration Only the standalone configuration
Standalone/network the database manager on a PC set up for
can be used. can be used.
viewing purposes. Note that LabSolutions UV-Vis
must be installed on the PC used for viewing.

Performed on a file-by-file basis

Data backup Performed for each database.
using Windows Explorer.

34
Database Management Prevents Mistakes
With LabSolutions DB UV-Vis System and LabSolutions CS UV-Vis System,
the analysis data are managed securely by the database. Overwriting, Analysis Postrun analysis Postrun analysis

deletion and other mistakes typical of data file management do not Revision number: 1 Revision number: 2 Revision number: 3
occur.
In addition, when postrun analysis is performed using the acquired data,
postrun analysis data revision numbers are automatically assigned,
Reduced operator errors
preventing the accidental overwriting of raw data.

Solid Security
An audit trail to ensure the reliability of data and document e-mail requirements. It is also possible to set lockout functions to prevent
transmission functions when any event occurs in the system can be set illegal access, and set a registered user’s deletion and change. In
up. User accounts are managed using passwords, where password addition, a box can be selected to prevent overwriting a data file, and
length, complexity and term of validity must satisfy specified outputting an item to a report can also be performed.

Pertinent Information Managed for Every Project

LabSolutions DB UV-Vis System and CS UV-Vis System provide a Management can be suited to tasks!
project management function enabling management suited to tasks
and system operations. This function enables equipment and user
management, security policy, and data processing to be set on a Proj ect A Project B
Lab So l u ti o n s
project-by-project basis, thereby improving the efficiency of data server
searches and management tasks.

Project
A ccess O K A
data Project
B
data A cce s s OK

Only shows data related to the project A u th o ri zati o n

for more convenient data searching.

Visualization of the Sequence of Analysis Operations

Creating a report set** provides visibility of the individual analytical
operations involved in the overall analytical process. When analytical
operations are visible, it is easier to check for operating errors, which ** Report sets include test methods and test results for a series of samples analyzed, and also a
corresponding operation log (a record of all operating events from login to logout), which is
helps improve the efficiency and reliability of checking processes. automatically extracted from the data and summarized in a single report.

Sample information Analysis condition

Report Set**
Operation history Results
Data File

UV-VIS Series Accessories

UV-VIS Spectrophotometers Accessories 35
 UV-VIS Series Accessories

For Research Use Only. Not for use in diagnostic procedures.

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