Scribd
Upload
45 views2 pages

Bmet 442

The document is an exam for a second year biomedical degree assessing knowledge of recombinant DNA technology. It contains 4 questions, with the first being the most comprehensive asking students to describe applications of restriction enzymes and hybridization, essential features of cloning vectors, and factors contributing to successful PCR. Students must also discuss applications of recombinant DNA technology. The other questions focus on uses of bioinformatics, industrial applications of the technology, Northern blotting and its application, shot gun sequencing, producing labeled probes, and cloning vectors.

Uploaded by

Stephen Siengo
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
45 views2 pages

Bmet 442

The document is an exam for a second year biomedical degree assessing knowledge of recombinant DNA technology. It contains 4 questions, with the first being the most comprehensive asking students to describe applications of restriction enzymes and hybridization, essential features of cloning vectors, and factors contributing to successful PCR. Students must also discuss applications of recombinant DNA technology. The other questions focus on uses of bioinformatics, industrial applications of the technology, Northern blotting and its application, shot gun sequencing, producing labeled probes, and cloning vectors.

Uploaded by

Stephen Siengo
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 2

BMET 442

CHUKA UNIVERSITY

UNIVERSITY EXAMINATIONS

SECOND YEAR EXAMINATION FOR THE AWARD OF DEGREE OF BACHELOR


OF SCIENCE IN BIOMEDICAL

BMET 442: RECOMBINANT DNA TECHNOLOGY

STREAMS: TIME: 2 HOURS

DAY/DATE: TUESDAY 23/03/2021 11. 30 A.M – 1.30 P.M

INSTRUCTIONS:

Answer question one and any other two questions

Do not write on the question paper

QUESTION ONE ( 30 MARKS)

(a) Describe the main applications of restriction enzymes. [6 marks]

(b) Describe briefly the areas in which hybridization can be used. [6 marks]

(c) Highlight essential features of a cloning vector. [6 marks]

(d) There are several factors that contribute to a successful PCR process. Briefly discuss any six.
[6 marks]

(e) Discuss the application of recombinant DNA technology. [10 marks]

QUESTION TWO (20 MARKS)

(a) Explain various uses of bioinformatics. [10 marks]


(b) Give various examples of industrial applications of recombinant DNA technology.
[10 marks]

Page 1 of 2
BMET 442

QUESTION THREE (20 MARKS)

(a) With the aid of an illustration, explain the process of Northern Bottling and its
application. [10 marks]
(b) Short gun sequencing is used to sequence very large amounts of DNA .Discuss.
[10 marks]

QUESTION FOUR (20 MARKS)

(a) Explain in detail the various methods for the production of labeled probes. [10 marks]
(b) Explain various cloning vectors used in recombinant DNA. [10 marks]

------------------------------------------------------------------------------------------------------------------------------------------

Page 2 of 2

You might also like