Food Test Lab
Uploaded by
LillyFood Test Lab
Uploaded by
LillyObjectives for food tests:
Test for reducing sugars:
1.
Test for non-reducing sugars:
1.
Test for starch:
Test for lipids:
Test for proteins:
Materials/Apparatus:
- Test tubes
- Test tube rack
- Water bath
- Pipette
Food items:
- Energy drink
- Glucose solution
- Water
- Cooking oil
- Starch
- Potato
- Fruit juice
- Corn
- Cheese
- Chicken
- Peanut butter
- Sucrose solution
- Cucumber
- Sausage
- Banana
- Pasta
- Apple
Reagents:
- Benedict’s solution
- Iodine
- Ethanol
- Biuret solution
- Sodium hydroxide
- Hydrochloric acid
Method:
Testing for Reducing sugar:
1. 3 test tubes were placed in the test tube rack.
2. 5ml of the energy drink was added to one test tube. This was repeated in separate test
tubes with the glucose solution and water.
3. A few drops of benedicts solution were added to each test tube.
4. All of the test tubes were placed in a water bath of 80-100◦C for 5 minutes.
5. Observations were made and recorded.
Testing for non-reducing sugar:
1. 3 test tubes were placed in the test tube rack.
2. 10ml of water was added to one of the test tubes.
3. 10ml of sucrose solution was added to the two remaining test tubes.
4. A few drops of hydrochloric acid were added to the test tube with water and one with the
sucrose solution.
5. The test tubes with the sucrose solution and hydrochloric acid along with the test tube
containing water and hydrochloric acid were placed in the water bath at 70℃ for 3
minutes.
6. 10 drops of sodium hydroxide were added to the solutions with hydrochloric acid .
7. A few drops of benedict’s solution were added to each test tube.
8. All the test tubes were placed in the water bath at 70℃ for 3 minutes.
9. Observations were made and recorded.
Testing for starch in liquids:
1. 4 test tubes were placed in the test tube rack.
2. 20ml of each food item (egg white, fruit juice, starch, water, and cooking oil) was added
to its own test tube.
3. A few drops of iodine were added to each test tube.
4. Observations were made and recorded.
Testing for starch in solid food:
1. The cucumber, sausage, banana, corn, chicken, cheese, pasta, apple, potato, and peanut
butter were placed on the paper towel roughly 3 inches apart.
2. A few drops of iodine solution were added to each food sampled.
3. Observations were made and recorded.
Testing for lipids:
1. 4 test tubes were placed in the test tube rack.
2. 15ml of sample A was added to one while 15ml of sample B was added to the other.
3. 15ml of water was added to the remaining test tubes.
4. 20ml of ethanol was added to each test tube, a clean thumb was placed over the top and
they were shaken vigorously.
5. The solution with sample A was placed into one of the test tubes with water, this process
was shortly repeated with sample B.
6. Observations were made and recorded.
Testing for proteins:
1. 4 test tubes were placed in a test tube rack.
2. 30ml of Egg white, starch, cooking oil, water and fruit juice were each added to their own
test tubes.
3. A few drops of biuret solution were added using a pipette to each test tube.
4. Observations were made and recorded.
Observations:
TABLE SHOWING OBSERVATIONS/RESULTS FOR BENEDICTS TEST FOR REDUCING
SUGAR
Solution Result
Water No changes observed, light blue colour
retained
Glucose solution Brick red solution formed
Energy drink Orangey-red solution formed
TABLE SHOWING OBSERVATIONS/RESULTS FOR BENEDICTS TEST FOR NON-
REDUCING SUGARS
Solution Result
Water, Hydrochloric acid, and Sodium The colour of the solution turned light blue.
Hydroxide No reaction occurred.
Sucrose solution, Hydrochloric acid, and The colour changed from light blue to a milky
Sodium hydroxide brownish yellow.
Sucrose Solution A slight colour change from light blue to light
green.
TABLE SHOWING OBSERVATIONS/RESULTS FOR STARCH TEST IN LIQUIDS
Substance Result
Water Bright yellow
Cooking oil Bright yellow
Egg white Bright yellow
Starch Black
Apple Juice Bright yellow
TABLE SHOWING OBSERVATION/RESULTS FOR STARCH IN SOLID FOOD
Food item Observation
Cucumber No reaction
Sausage No reaction
Banana black
Chicken no reaction
Corn Black
Cheese Blue-Black
Pasta Black
Apple No reaction
Potato Black
Peanut butter Blue-black
TABLE SHOWING OBSERVATIONS/RESULTS FOR LIPIDS TEST
Sample Result
A A milky white emulsion formed on top of the
water.
B No changes were observed
TABLE SHOWING OBSERVATIONS/RESULTS FOR PROTEIN TEST
Substance Observation
Egg white Purple/Violet Colour
Cooking oil Floated on top of solution which remained
light blue
Water Light blue
Fruit juice Changed from yellow to green
Starch Blue
