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ZOO LAB Activity 2 Module 2.

This document provides instructions for a zoology lab exercise on basic microscopy. It identifies the 14 main parts of a microscope and their functions. These include the body tube, nosepiece, objectives, stage, adjustment knobs, base and others. The document also calculates the total magnification power when using different objectives at 10x ocular magnification. It notes that objects appear inverted and move in the opposite direction when the slide is moved. Finally, it defines a parfocal microscope as one that remains in focus when the objective is rotated.

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JAN GABRIELLE V MOLINA
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38 views

ZOO LAB Activity 2 Module 2.

This document provides instructions for a zoology lab exercise on basic microscopy. It identifies the 14 main parts of a microscope and their functions. These include the body tube, nosepiece, objectives, stage, adjustment knobs, base and others. The document also calculates the total magnification power when using different objectives at 10x ocular magnification. It notes that objects appear inverted and move in the opposite direction when the slide is moved. Finally, it defines a parfocal microscope as one that remains in focus when the objective is rotated.

Uploaded by

JAN GABRIELLE V MOLINA
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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School of Arts and Sciences

Exercise No.2 in NSE 2- Laboratory


Zoology Lab
First Semester | AY 2022-2023

Module 2 Task: Basic Microscopy


A. Identify the Parts of the microscope

1. Body tube 8. Lamp


2. Nosepiece 9. Ocular lens (Eye piece)
3. Low power objective 10. Arm
4. Medium power objective 11. Stage
5. High power objective 12. Coarse adjustment knob
6. Stage clips 13. Fine adjustment knob
7. Diaphragm 14. Base

Faculty: Dra. Menchu C. Luzano Page 1 of 3


School of Arts and Sciences
Exercise No.2 in NSE 2- Laboratory
Zoology Lab
First Semester | AY 2022-2023

B. Give the function of each part (follow the numbering on the microscope).
1. Body tube- It keeps the optics 8. Lamp- it provides light for us to see
continuously aligned by separating the things in the scope
objective and the eyepiece.
2. Nosepiece- it holds two or more 9. Ocular lens- It enlarges the image
objective lenses and can be rotated to created by the microscope's objective so
easily change power. that the human eye can see it.
3. Low power objective- scanning 10. Arm- It attaches to the base and holds
numerous smaller specimens or inspecting up the microscope head.
huge specimens. (4x)
4. Medium power objective- It is attached 11. Stage- It holds all the lenses above the
to the nosepiece and magnifies 10x stage.
5. High power objective- it is perfect for 12. Coarse adjustment knob- it allows for
viewing fine details for the specimen a fast focusing by moving the stage or
sample objective lens up and down
6. Stage clips- it holds the slides in place. 13. Fine adjustment knob- it focus the
image very fine at the higher magnification
7. Diaphragm- it controls the amount of 14. Base- it holds the bottom of the
light entering the scope. microscope for it to stand.

C. When using different objectives (LPO, HPO, OIO, and Scanning) objects are magnified at
different size. Compute for the total magnification power of each objective Consider the ocular
magnification is 10x:

Total magnification of Scanner Total magnification of LPO

(4x)(10) = 40x (10x)(10x) = 100x

Total magnification of HPO Total magnification of OIO

(40x)(10) = 400x (100x)(10) = 1000x

What happen to the image being viewed when If we move the slide in focus to the left it
you move the slide in focus to the left? will seem right side

Faculty: Dra. Menchu C. Luzano Page 2 of 3


School of Arts and Sciences
Exercise No.2 in NSE 2- Laboratory
Zoology Lab
First Semester | AY 2022-2023

What happen to the image being viewed when If we move the slide in focus to the right
you move the slide in focus to the right? the image will appear to move to the left

What happen to the image being viewed when If we move the slide in focus upward the
you move the slide in focus upward? image will appear to move downward
What happen to the image being viewed when If we move the slide in focus downward the
you move the slide in focus downward? image will appear to move upward

What is your generalization about the All objects or particles are scanned through
position and movement of images when microscope are appearing to be inverted
viewed under the microscope? and opposite to the true position that we
can see

D.
When do we say that the microscope is When it is focus in a one objective and
parfocal? when the objective is rotated it mostly
remain in to focus

Faculty: Dra. Menchu C. Luzano Page 3 of 3

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