URIT®

Maintenance Manual
of URIT-5380 Five-Part-
Diff Automated
Hematology Analyzer

URIT Medical Electronic CO., LTD.

 CONTENTS
COPYRIGHT AND DECLARATION...................................................................................................I

CHAPTER 1 INTRODUCTION................................................................................................1

1.1 FRONT VIEW....................................................................................................................1

1.2 REAR VIEW......................................................................................................................1
1.3 FUNDAMENTALS OF TEST................................................................................................2
1.3.1 Cell Counting Principle of Electrical impedance................................................2
1.4 WBC CLASSIFICATION PRINCIPLE.................................................................................3
1.4.1 Optical Classification Principle.............................................................................3
1.5 RBC TEST PRINCIPLE OF ELECTRICAL IMPEDANCE....................................................4
1.5.1 Test Principle of Total Number of RBC...............................................................4
1.5.2 Test Principle of RBC Indexes...............................................................................5
1.6 TEST PRINCIPLE OF PLT.................................................................................................5
1.7 TEST PRINCIPLE OF HGB...............................................................................................6

CHAPTER 2 PRECAUTIONS...................................................................................................7

2.1 ENVIRONMENTAL REQUIREMENTS.................................................................................7

2.1.1 VOLTAGE PROBLEMS..........................................................................................................7
2.1.2 Electromagnetic Interference........................................................................................7
2.1.3 Temperature....................................................................................................................7
2.2 DISPLAY REQUIREMENTS................................................................................................7
2.3 BOOT NOTES....................................................................................................................7
2.4 BLOOD SAMPLING AND TESTING....................................................................................8

CHAPTER 3 CIRCUIT...............................................................................................................9

3.1 INTRODUCTION................................................................................................................9
3.1.1 Circuit frames.........................................................................................................9
3.1.2 ARM board...........................................................................................................11
3.1.3 Optical acquisition board.....................................................................................11
3.1.4 CPU board.............................................................................................................12
3.1.5 Front-end Amplifier Board..................................................................................13
3.1.6 ADFIFO Board.....................................................................................................14
3.1.7 Solenoid Valve Board...........................................................................................15
3.1.8 Vacuum Board......................................................................................................16
3.1.9 Motor Driver Board.............................................................................................16
3.1.10 Pressure plate........................................................................................................19
3.1.11 LMS Counting Board...........................................................................................20
3.1.12 Amp Power Board (Analog Power Board).........................................................21
3.1.13 Power Adapter Board...........................................................................................22
3.1.14 Liquid Optocoupler Detection Plate...................................................................22
3.1.15 Auto Sampling Drive Board & Auto Sampling Control Board.......................23

CHAPTER 4 FLOW SYSTEM.................................................................................................24

 Contents

4.1 SYRINGE MODULE.........................................................................................................26

4.2 SAMPLE CUP..................................................................................................................27
4.3 FLOW DIAGRAM.............................................................................................................30
4.3.1 Flow System of Pressure Module........................................................................31
4.3.2 Optical Flow System.............................................................................................31
4.3.3 Impedance Flow System......................................................................................32

CHAPTER 5 OPTICAL SYSTEM...........................................................................................33

5.1 OPTICAL STRUCTURE....................................................................................................33

5.2 OPTICAL SCHEMATIC....................................................................................................34

CHAPTER 6 TEST....................................................................................................................35

6.1 VALVE TEST...................................................................................................................35

6.2 GAIN ADJUSTMENT........................................................................................................35
6.2.1 Gain Adjustment of RBC and WIC....................................................................36
6.2.2 PLT Gain Adjustment..........................................................................................37
6.3 MOTOR TEST..................................................................................................................38
6.4 VALUE MODIFICATION..................................................................................................40
6.5 OPTICAL DEBUGGING....................................................................................................40
6.6 OPTICAL DEBUGGING....................................................................................................41
6.7 SOFTWARE UPGRADE.....................................................................................................41
6.8 SOFTWARE RESTARTING................................................................................................41
6.9 SKIP SELF-CHECKING....................................................................................................42

CHAPTER 7 UPGRADE PROCESS.......................................................................................43

7.1 UPGRADE PROCESS OF FLOW PROGRAM.....................................................................43

7.1.1 Preparation...........................................................................................................43
7.2 BIOS UPGRADE.............................................................................................................45

CHAPTER 8 TROUBLESHOOTING.....................................................................................48

8.1 OPTICAL FAULT.............................................................................................................48

8.1.1 Stains on WOC Flow Cell....................................................................................48
8.1.2 Stains on Image Forming Lens...........................................................................49
8.2 CHANGE WOC FLOW CELL.........................................................................................50

400 Hotline：400-727-2288........................................................................................................55
 Copyright and Declaration
We owns the copyright of this unpublicized issued manual, and has right to handle
as secret information. This manual just used as reference for operation, maintenance and
service of our product. Other personnel has no right to publish this manual.
This manual includes special information protected by copyright law. Copyright
reserved, prohibit copy and transmit any content of this manual against not through
written agreement by us.
We don’t make any formally guarantee for this manual, including (but not limit to)
implied guarantee responsibility on marketability and propriety lodged for certain
purpose. We without responsibility for the error included in this manual and indirectly &
abiogenetic damage that is caused by actual representation & usage provided by this
manual.
Content in the manual can be changed without giving notice.
Applicable model: URIT-5380, URIT-5380, URIT-5381

Our obligation：
We only responsible for instrument security, reliability and capability under following
condition：
Performed assemble, extend, re-debugging, improve and repair by our authorized
personnel;
Relevant wiring equipment accord with national standard;
Use the analyzer according to this service manual.
NOTE：
This analyzer cannot be used in family.
WARNING：
If each hospital or institution that is responsible for using this instrument cannot
realize a set of satisfactory service procedure, will cause deviant invalidation of
instrument, even jeopardize to health of human body.
Nowadays, We will provide relevant technical information conditionally when
customer request. In addition, narrate calibration method and other information through
list to help eligible technician to repair our instrument.

I
 Copyright and Declaration

Guarantee
Manufacturer techniques and material
We guarantees automated hematology analyzer no techniques and material problem
within one year from shipping day if under normal use and maintenance.
Free service
Our obligation under this guarantee not include freight and other fare, not
responsible for direct, indirect and ultimate damage & delay caused by following
condition: improper use, replaced accessories or repaired by personnel not authorized by
us.
This guarantee is not applicable for following items: instrument which is not through
maintenance or already broken; We original nameplate or is replaced or tore off; our
other product.
Security, reliability and run status
If following conditions occur, We are not responsible for the security, reliability and
run status of the analyzer：
Disassembly, stretch and re-debugging;
Serviced or changed not by our authorized personnel .
Send back instrument
If it’s needed to send back the instrument, please contact with distributor to get
detailed information, inform the analyzer serial number which marked on nameplate, we
will not accept if S/N cannot be identified. Please mark instrument No. and S/N, briefly
state the reason on sending back instrument.
Freight: if send back instrument for service, purchaser bears the freight (including
custom fare)

Version: 03/2016

II
 Chapter 1 Introduction

1.1 Front View

C
O
M
drain count
Ground
column

switch Power cord

connector

Figure1-1 Front View

1.2 Rear View

Cooling fan

nameplate

Figure1-2 Rear View

1
 Chapter 1 Introduction

1.3 Fundamentals of Test

URIT-5380 achieves WBC differential count with 4 angle laser light scattering
technique and obtains the blood cell analysis via three independent detection channels.
1) WBC/DIFF channels: achieves WBC count and classification with laser light
scattering technology in the WOC. Complete WBC count and classification in
one channel.
2) WBC/HGB channels: Hemoglobin and WBC testing by Colorimetry
3) RBC/PLT channels: RBC and PLT counting by Electrical impedance

1.3.1 Cell Counting Principle of Electrical impedance

Electrical impedance of wed blood cells (WBC) count principle which is based on the
principle of non-conductive causes resistance change when blood cell granules in
diluents go through the aperture. Take it as the basis for testing to count WBC and
determine its column.

Constant current source

Counting chamber

External electrodes
Internal electrodes
Outer chamber
Inner chamber

Cell suspension Aperture

Figure1-3 Electrical impedance

Inner and outer electrodes are placed inside and outside the room in the counting
chamber. The two chambers are separated by a ruby aperture with a diameter of 100μm.
The rear chamber is filled with a certain concentration of cell suspension, and the front
chamber is filled with diluents.
The cell conductivity which is lower than diluents conductivity is the relative poor
conductor. When a cell granules in front chamber goes through the aperture, it generates
an instantaneous pulse voltage between inner and outer electrodes. The number of
pulses is proportional to the number of cells. Pulse height is proportional to the size of
the cell volume. Under the influence of negative pressure, a certain capacity of the cells

2
 Chapter 1 Introduction

will continue through the aperture, thereby generating a series of pulses. Send to count
for obtaining a certain volume of total cells by pulse signals amplification, threshold
adjustment, identification, shaping and A / D conversion. (See Figure 1-3)）

1.4 WBC Classification Principle

URIT-5380 not only calculates the overall amount of WBC, but also offers graphics
leukocyte distribution - the scatter plot.（See Figure 1-4）

Monocytes
Neutrophils

Hidden Eosinophils
Cells Eosinophils

Basophils
s
Lymphocytes

Figure1-4 Scatter Plot

When doing a normal human blood test by URIT five classification analyzer, scatter
plots of most of samples should be like below figure. There’s clear cell grouping. In DIFF
channel, the gray part which is the shadow cell area is the reflection in the scatter plot
after the RBC dissolved in the sheath. (some people has it and some do not have it.) the
green is the lymphocytes, pink area is the mononuclear cells, blue area is the
neutrophils, white area is the basophils group and the red area is the eosinophil group.
There are obvious visible boundaries between each area. Cells with the same color come
into group, and cells with different color separates.

1.4.1 Optical Classification Principle

3
 Chapter 1 Introduction

Figure 1-5 Optical Schematic

Classification principles:
0 ° rake angle light scattering (1 ° ~ 3 °) Roughly determine cell size
90 ° polarization extinction scattering (70 ° `~ 110 °), based on the characteristics of
polarized laser vertical angle depolarization, separates the eosinophils from neutrophils
and other cells.
10 ° narrow angle light scattering (7 ° ~ 11 °) tests cell structure and relative
characteristics of complexity.
It can be simply understood as: 0 ° reflects the volume; 90 ° reflect lobocytes
situation; 10 °reflects both of above mentioned information.

Figure 1-6 WBC Feature Comparison

Steps of WBC classification
STEP 1: with a 90 ° angle to distinguish lobocytes cells and monocytes get two
categories, namely, 1 neutrophils and eosinophils (lobocytes cells) 2 monocytes and
lymphoid and basophils (monocytes).
STEP 2: With a 90 ° polarization distinguish eosinophils and neutrophils.
STEP 3: According to the nuclear-cytoplasmic ratio and cell size, with 0°and
10°distinguish basophils from lymphoid and mononuclear cells.
STEP 4: According to the size, with 0 °distinguish monocytes and lymphatic.

1.5 RBC Test Principle of Electrical Impedance

1.5.1 Test Principle of Total Number of RBC

The test principle of RBC is the same as that of WBC. Cells arranged in a certain
capacity go through aperture (68μm) under the negative pressure. Pulse is formed
during this process. The total number and average volume of RBC are obtained

4
 Chapter 1 Introduction

according to pulse size and height. The RBC volume distribution histogram is shown in
Figure 1-7.
Normally, ratio of number of RBC and WBC is approximately 750:1, so it can ignore
factors caused by WBC as testing the RBC. However, in some special pathological
conditions, such as leukemia simultaneously with blood disease, may cause
abnormal RBC count.

Figure1-7 RBC Atlas

1.5.2 Test Principle of RBC Indexes

HCT=(MCV × RBC) /10. According to the relevant algorithm, the MCH and MCHC
can be derived by RBC, MCV and HGB. RDW is obtained as testing RBC number and
volume differences, which reflects the outer periphery of RBC volume heterogeneity.
RDW which reflects the extent of RBC sizes has clinical significance for diagnosis of
anemia.

1.6 Test Principle of PLT

Platelet (PLT) and RBC are tested in the same count chamber. The analyzer
respectively counts it according to different thresholds. (See Figure 1-8)
PLT data stores in 64 channels in 2 ~ 30fL.

Figure1-8 PLT Atlas

PDW is obtained according to the histogram and computer processing. MPV is the

5
 Chapter 1 Introduction

groups arithmetic average volume of PLT histogram curve. Normal MPV and PLT
amounts is non-linear negative correlation. PCT is drawn through the MPV and PLT.

1.7 Test Principle of HGB

Hemoglobin (HGB) and WBC counts in the same counting chamber. In WBC
counting chamber, the lyse destroys RNC in the blood and the HGB is dissolved out.
Colorimetric assay in specific wavelength (540nm) in count chamber, absorbance change
is proportional to HGB content in liquid. HGB test results is obtained by correlation
algorithm.

6
 Chapter 2 Precautions

2.1 Environmental Requirements

2.1.1 Voltage Problems

To ensure the normal work and stable test, the analyzer uses 220V power input.
High-precision automatic AC power supply should be installed as the electric supply is
unstable. If intermittent power outages happens frequently, please install the UPS
uninterruptible power supply, so as to avoid damage to the power and circuit board.

2.1.2 Electromagnetic Interference

Acquisition signal is very weak, external interference may cause abnormal data
Therefore, it’s recommended connecting with ground wire to avoid affecting the test
results by interference signal. Away from the equipments generated interference signals,
such as monitors, copiers, centrifuges and X-ray detector.

2.1.3 Temperature

The required operating temperature is 15℃~35℃. Temperature is too low which

affects the reagents and test data. The most common is that hemolysis becomes slow
because of low temperature, resulting in unusually high of WBC and HGB. PLT
aggregates together because of low temperature, which makes low PLT data.

2.2 Display Requirements

1、 Place the analyzer and reagents in the same horizontal plane to ensure reagent
can be quickly added into the analyzer.
2、 Waste containers should be placed on the ground. (Avoid waste overflowing)
3、 Insert the reagent connectors. Diluents connect with the blue one, detergent
connects with the green one and sheath connects with the yellow one.
Note: The analyzer is a precision optical instruments, pay attention to dust
when used.

2.3 Boot Notes

1、 Check whether the tubing connector of flow system looses or cracks. If so,
please deal with it before boot.
2、 After boot, check whether there’s abnormal sound or smell, the screen display is
7
 Chapter 2 Precautions

normal or not. If so, please shut down the analyzer immediately and check it.

3、 Check whether the screen display and program initialization is normal. Enter
sample test interface if it’s normal.

2.4 Blood Sampling and Testing

There are two sample test modes, which are whole blood and pre-dilution.
1、Whole blood collection: collecting human blood by vacuum blood collection. The
anticoagulant in the collection tube anticoagulats the blood sample.
2 、 Pre-dilution collection: collecting human peripheral blood with blood collection,
such as fingers, ears and so on.
3 、 Whole blood mode test: in count interface, inject sample probe into the
anticoagulant tube and then click START to test.
4、Pre-dilution mode test: put the disposable tubes in the STAT position and press
Drain (or click in the interface), then 800ml diluent is injected into the disposable
tube. Collect and inject 20μL peripheral blood into the tube and mix it. Place this
tube in the STAT position again and click COUNT to start testing.

※Note: avoid squeezing when collecting peripheral blood so as not to extrude tissue
fluid or aggregate PLT, which may affects PLT counting. Needle goes a little bit
deeper when collecting peripheral blood. Do not collect first drop of blood as
sample.

8
 Chapter 3 Circuit

The circuit consists of Dual-supply switching power supply, ARM board, CPU board,
ADFIFO board, front-end amplifier board, auto sampling drive board, auto sampling
control board, motor driver board, vacuum panels, photovoltaic acquisition board, power
adapter plate, level detection optocoupler board, Amp Power board (analog power supply
board), USB converter board, IC card reader board, pressure plate, LMS plate, solenoid
valve plate, photomultiplier tube mounting plate and panel indicator board.
NOTE: the ARM board and CPU board shall be combined into one circuit board.

3.1 Introduction

3.1.1 Circuit frames

Positive pressure tank

Waste tank

LMS Diluent tank

board

Vacuum pump

Auto sampling
drive board

Figure3-1 Left Door

9
Sample Reagent
cup interfaces
 Chapter 3 Circuit

ADFIFO board
Vacuum board

solenoid
valve plate
Card reader (5 PCS)

optocoupler detection ARM board

plate

CPU board

Auto sampling
control board
ARM board

front-end
amplifier board

Figure 3-2 Right Side View

pressure
plate Willinge
n laser

Count vacuum
chamber

power adapter plate

Injector module

Dual-supply switching Amp Power board (analog

power supply power supply board)

Figure 3-3 Rear

10
 Chapter 3 Circuit

3.1.2 ARM board

ARM board which is the control center of the analyzer stores gain and motor steps.
D250 twinkles when ARM board works in normal. (Figure 3-4)

Flashing light in normal operation

Flashing light in normal operation

Figure 3-4 ARM board

NOTE: the ARM board and CPU board shall be combined into one circuit board.

11
 Chapter 3 Circuit

3.1.3 Optical acquisition board

Collect optics 0 °, 10 ° laser signal and convert it into an analog signal to ADFIFO
board.

Voltage detection
point in blank test

Connect signal line

of ADFIFO plate

Figure 3-5 Optical acquisition board

3.1.4 CPU board

CPU control board which is responsible for system logic control provides various

parameters for the ARM industrial control panels and executes the command.

See Figure 3-6.

12
 Chapter 3 Circuit

Connect ADFIFO board

Panel light indicator board

Digital 5V

Card reader COM1

Connect front-end amplifier board port

V33-
V342

V25-V32 Auto sampling

drive board
Liquid optocoupler
V17-V24 detection
plate （ SEN1 ）

V9-V16

Motor driver board

SEN2
V1-V8

LMS board
Vacuum
plate （ SV ）
Figure 3-6 CPU board

3.1.5 Front-end Amplifier Board

Front-end amplifier board amplifies and processes weak cellular signal of sample
cups and adjusts it to the appropriate signal to the ADFIFO board for data conversion.

13
 Chapter 3 Circuit

Connect CPU board

Connect ADFIFO
board

Offer +/-12v

HGB
Interface Offer AC100V
burning, DC100V
constant current
source
WBC Interface

Figure 3-7 Front-end amplifier board

RBC Interface

3.1.6 ADFIFO Board

It’s mainly used for A / D digital-analog conversion.

Connect front-end
amplifier board 0°Interf 10°Interf 90°Interfa 90°D
ace ace ce Interface

the light intensity

flashes when there Flashing lights in
has impedance normal work
signal

Connect CPU board

14
 Chapter 3 Circuit

Figure 3-8 ADFIFO Board

NOTE: this ADFIFO board is replaced by the ADFIFO board with new version.

3.1.6.1 ADFIFO Board Test Points

Figure 3-9 Test Points of ADFIFO Board

1、WBC test point 2、HGB test point 3、RBC test point 4、PLT test point
5、0°test point 6、10°test point 7、90°test point 8、90°D test point 9、A+12V
test point （ the lights lit on standby ） 10 、 A-12V test point （ the lights lit on
standby ） 11 、 AGND test point 12 、 ADC+3.3V （ the lights lit on standby ）
13 、Flashing lights on standby 14、A+5V Indicator（the lights lit on standby）
15、D+5V Indicator（the lights lit on standby） 16、F+3.3V Indicator（the lights
lit on standby）

3.1.7 Solenoid Valve Board

Solenoid valve board controls valve switches in flow system and change flow
direction, which ensures unblocked flow system.

15
 Chapter 3 Circuit

Connect Connect valve/pump

DC12V

Figure 3-10 Solenoid Valve Board

3.1.8 Vacuum Board

Vacuum board which is responsible for controlling the vacuum pump tests liquid-
storage tank, the waste tank, vacuum tank and internal pressure of pressure tank.（See
Figure3-14）

U3 （ connect vacuum counting chamber ）

U1 （ connect Positive pressure

chamber ）

U4 (connect pressure chamber)

Connect CPU board +12V input

Figure 3-11 Vacuum board

16
 Chapter 3 Circuit

3.1.9 Motor Driver Board

Motor driver board is mainly responsible for the movement of each syringe
movement and sampling unit, and testing whether the syringes and sampling unit are in
place.

C5 ： +12V/5V
input
MA Motor

MB Motor Lights of optocoupler, from top to bottom,

it’s respectively
MA 、MB 、MC 、MD 、ME 、MF
MC Motor

MD Traverse motor of sampling

unit

ME Longitudinal motor of sampling unit

MF sample probe
From left to right, it’s
SA 、SB 、SC 、SD 、SE 、SF

Figure 3-12 Motor Driver Board

Figure 3-13 Auto Sampling Module 1

17
 Chapter 3 Circuit

Figure 3-14 Auto Sampling Module 2

Figure 3-15 Auto Sampling Module 3

18
 Chapter 3 Circuit

Figure 3-16 Sampling

3.1.10 Pressure plate

Pressure plate which provides DC600V hypertension to photomultiplier (PMT) has a

direct affect towards classification of optics 90 ° and 90 ° D. Measurement method is to
measure interface voltage of PMT directly (DC600V), or measure shield voltage of last
stitch of ADFIFO board connector interface (DC6V). (See Figure 3-17)

19
 Chapter 3 Circuit

voltage 6V shield voltage of

last stitch of
ADFIFO board
connector
voltage 6V interface (DC6V)

DC600V, connect
PMT
Connect
PMT base

Figure 3-17 Pressure Plate

3.1.11 LMS Counting Board

Counting time measurement module consists of 1 LMS board and 2 glass tubes.
There are 4 optpcouplers and 4 potentiometers. These 4 optocouplers correspond test
points TEST1-TEST4 respectively. The voltage is 4.8 ± 0.2V as the glass tube filled with
liquid, and the voltage is 2.9 ± 0.1V as the glass tube is empty. Optocoupler parameter
deviation and dirty inner-wall of glass tube shall cause the voltage deviation of TEST1-
TEST4.
LMS board calculates the injected liquid via optpcoupler and metering tube detection
so as to ensure measurement accuracy of WBC, RBC and PLT. The measuring board
has two channels, one is the WBC channel, and the other is the RBC and PLT channel.
Each channel consists of 1 metering tube and 2 optpcouplers. Open the V33 and V34
before counting. The air goes into the WBC and RBC metering tube of LMS board.
Empty the liquid in the tube, close the V33 and V34 after counting, the liquid goes
through aperture and metering tube. The liquid column of metering tube moves down.
Comparator inputs counting signal as liquid column meets the up optocoupler, and the
comparator stops inputting counting signal as the liquid column meets the down
optocoupler.

20
 Chapter 3 Circuit

start optocoupler in
RBC count
start optocoupler in
WBC count

End optocoupler Adjustable potentiometer

in RBC count in
whole blood mode
and pre-dilution
mode

End optocoupler in WBC

count in whole blood mode
and pre-dilution mode

Figure 3-18 LMS Counting Board

3.1.12 Amp Power Board (Analog Power Board)

Offer Offer
+/-12V +5V

Offer AC100V Cauterize,

DC100Vconstant current
source

+12V input Offer +/-12V, 5V

Figure 3-19 Amp Power Board

21
 Chapter 3 Circuit

3.1.13 Power Adapter Board

24V/12V 12V/5V 5V output GND 12V output

input input

Figure 3-20 Power Adapter Board

3.1.14 Liquid Optocoupler Detection Plate

Sen1 connect CPU board

S6 ： Diluent S7 ： lyse detection

detection

S5 ： diluent S8 ： sheath detection

detection
S4 ： detergent detection BNC

S3 ： sheath detection S15 ： Level detection of

diluent

S2 ： lyse detection

S1 ： diluent detection

Sen2 connect CPU board

Figure 3-21 Liquid Optocoupler Detection Plate

22
 Chapter 3 Circuit

3.1.15 Auto Sampling Drive Board & Auto Sampling Control Board

The auto sampling drive board (Figure 3-22) auto sampling control board (Figure 3-
23) are used to control the auto sampling module. There’s a LED light on the top of S1-
S14 of auto sampling drive board and auto sampling control board. the LED lights/lights
off when the optocoupler guard sheet is ready.

Connect auto sampling drive board 12V\5V input

M1 M2 M3
From left to right, S1—S8

Figure 3-22 Auto Sampling Drive Board

Connect auto sampling drive board MG(Peristaltic

M5 M6 M7 pump)

S13

S12 Connect CPU board

S11

S10 条码仪接口
S14
S9

+12V +5V 12V\5V output to auto hot key of

input input sampling drive board front shell
自动进样驱动板
Figure 3-23 Auto Sampling Control Board

23
 Chapter 4 Flow System

Frame diagram of flow system in front view is shown as below.

Positive LMS Board

pressure
chamber

Vacuum RBC
chamber cup

WBC
cup

Syringe module

WOC cup

Mix
Diluents storage chamber
tank

Figure 4-1 Left Perspective Figure 4-2 Front View

24
 Chapter 4 Flow System

Positive
pressure
tank

S5-S8 liquid detection

optocoupler

Diluents storage
tank

Waste
tank

Sample
cup

S1-S4 liquid detection

Vacuum pump module
optocoupler

Figure 4-3 Left View

Card
WOC reader
Interfaces of
optical
system

Peristalti
c pump Sampling
unit

STAT position
MC send the
sample to the
WOC

25
 Chapter 4 Flow System

Figure 4-4 Front View

4.1 Syringe Module

As shown in Figure 4-5, the main function of it is cleaning, counting, priming, sample
dilution and offering diluents and power sources. The circuit board provides DC12V to
the motor.
Syringe module consists of a small syringe, sampling syringe, dilution syringe,
motors, seals and other components. Three kinds of syringe can be individually
disassembled for easy replacement of the entire syringe, or replace seals.
Motor of syringe module is installed in the rear of the syringe, which avoids electrical
corrode damaged caused by syringe leak.

MF （ 10ml ）
add
sheath to
WOC cup

MC sends
MA （ 100μL MA （ 2.5ml ） MB(10ml) gives
sample to
） sampling add lyse to diluents to WBC,
WOC
and WBCcup RBC, cleaning and
separating sample probe

Figure 4-5 Front View of Syringe Module

26
 Chapter 4 Flow System

MB MA
MF motor motor motor

Figure 4-6 Rear View of Syringe Module

NOTE: sampling and distinguishing syringe and lyse syringe use a same motor
MA.

4.2 Sample Cup

As shown in Figure 4-7, sample cup components which is the counting sensor of the
analyzer is the most front-end detection element of data acquisition.
Functionally, the sample cup consists of inner and outer electrodes, front and rear
chambers and ruby aperture.
Measure RBC, WBC and PLT parameters via Coulter principle (electrical impedance
principle). In the sample cup, the circuit provides a constant current through diluted
conductive liquid in cell counting. As cells go through aperture, the loop resistance
changes. Cells with different volume produce electrical pulses with different amplitudes,
so cells volume and numbers can be calculated.
Make a Colorimetric analysis towards the treated sample and calculate HGB value via
light emitting and receiving of WBC cup.
NOTE: the liquid should be sprayed on the walls of the cup, or the results of MCV, PLT
and HGB shall be affected.

27
 Chapter 4 Flow System

RBC cup WBC cup

(68μL) (100μL)

HGB test

Figure 4-7 Sample Cup

28
Chapter 4 Flow System

Figure 4-8 WOC Cup

29
 Chapter 4 Flow System

4.3 Flow Diagram

Figure 4-9 Flow Diagram

30
 Chapter 4 Flow System

4.3.1 Flow System of Pressure Module

Flow system of pressure module is responsible for providing pressure of 160KPa

and 78KPa, pumps reagent to the liquid reservoir and supplies it to the analyzer for
cleaning and counting and form sheath flow effect. See Figure4-10.

Figure 4-10 Flow System of Pressure Module

（1）air mix tank: V26 opens and apply air to the pump till the pressure goes to 118KPa.
Open the V9, V11 and V10 to make bubbles of WOC cup, WBC cup and RBC cup.
（ 2 ） positive pressure tank: open V26 and offer 160KPa pressure to the tank via V1,
which is used to a) offer WOC a 160KPa pressure for WOC calculating via V25 and
V2, b) offer recoil pressure to the cup via V22, V35 and V36.
（ 3 ） diluent storage tank: V24 is on work, open V28 and V27, turn off V14 and V25,
store the diluents in the tank for counting use.
（4）counting vacuum tank: V24 is on work, make inner pressure achieve to 78KPa.
（ 5 ） connect U1, U3 and U4 to the pressure sensor for monitor and control the inner
pressure.

4.3.2 Optical Flow System

Figure 4-11 Optical Flow System

Add 2000μl sheath into WOC cup by MF, use MA syringe to collect 30μl blood and
inject 8.5μl of it into WOC cup, and mix the blood and sheath in mixing cup. Open
the V4 and V8, pump the mixed liquid into the channel between V4 and V8 by
peristaltic pump, and inject it into WOC via MC syringe. The waste is pumped and
discharged by V24.

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 Chapter 4 Flow System

4.3.3 Impedance Flow System

Process explanation on impedance flow system ( see Figure 4-8）

（1）add lyse into WBC cup via V19. MB syringe which is used to absorb diluents into
WBC cup and RBC cup via V21, V20 and V18 and inject diluents into clean sets
and sample probe via V21, V20, V18 and V17.
（2）V16 and V40 pump the liquid which is used to clean sample probe via V29 pump,
and pump the liquid which is used to clean RBC and WBC cups via V31 and V32
pumps.
（3 ）MA (100μl) syringe is used to collect samples and give it to WOC cup and WBC
cup.
（ 4 ） the mix tank offers 115KPa pressure, the sample in WBC cup and RBC cup is
mixed via V10 and V11.
（ 5 ） the liquid goes through aperture and reaches glass tube via V35 and V36, the
count pressure tank offers 78KPa pressure, time counts by optocoupler of count
board.

Figure 4-8 Impedance Flow System

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Chapter 4 Flow System

33
 Chapter 5 Optical System

5.1 Optical Structure

Components of the optical system is shown below.

Figure 5-3 Optical Detection System

1— System Work Platform
2—Reflector
3—Cylindrical Mirror
4—Imaging Lens Group and Bracket
5—WOC Flow Cell
6—Forward Condenser Group and Bracket
7—PhotoAmp BOARD PCBA
8—Microscope objective components
9—700 Microns Slit and Bracket
10—Spectroscope and polarizer Bracket
11—90°PMT
12—90DPMT
13—639nm Semiconductor laser
14—high-volage switchboard

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 Chapter 5 Optical System

Figure 5-1 Components of the Optical System

5.2 Optical Schematic

Figure 5-2 Optical Schematic

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 Chapter 6 Test

6.1 Valve Test

Click ‘Service’ in Count interface, click ‘1111’ and ‘OK’ to enter valve test interface.
click valve number shown in below figure, the corresponding valve makes action.

Control of valves of flow system

Constant current
source switch

Figure 6-1 Valve Test

6.2 Gain Adjustment

Click ‘Service’ in Count interface, input ‘4444’ to enter gain adjustment interface.
press ‘Ctrl+H’ to see all parameters.
Input the value in the box at the right side of the need-to-be changed item and press
’Enter’. Click ‘Save’ at the bottom lest corner and exit. Please see the following figures
for details.

36
 From top to the bottom,
WBC 、RBC 、PLT 、0° 、10° 、90°
、90°D
TheChapter
larger 6
the value, the smaller
Test
the gain

Figure 6-2 Gain Adjustment（I）

Positive&negative pressure adjustment

Mix pressure adjustment

Figure 6-3 Gain Adjustment（II）

6.2.1 Gain Adjustment of RBC and WIC

Check the gain of RBC, WIC and PLT after testing by quality control material ( see
Figure 6-4), if it’s within QC requirements, there’s no need to adjust it. If not, please
adjust it in gain adjustment interface. Click ‘Service’ in Count interface, input ‘4444’ and

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 Chapter 6 Test

click first page （see Figure6-2）. Input those needed-to-be-changed value in the blank
box at the right side and press ‘Enter’. Click ‘Save’ before exit. Then do QC and check
whether the gain of RBC, WIC and PLT are within reference range. If not, please re-
modify till the gain in the reference range.

RBC gain target value

Figure 6-4 RBC Gain Adjustment

WIC gain target value

Figure 6-5 WIC Gain Adjustment

6.2.2 PLT Gain Adjustment

The specialized PLT QC is needed in PLT gain adjustment. The analyzer has been

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 Chapter 6 Test

adjusted before it leaves the factory.

Adjust the PLT gain as changing the front-end amplifier board. Do a sample test and
adjust the PLT gain which should be the same as it before changing.

PLT gain target value

Figure 6-6 PLT Gain Adjustment 1

Test with specialized PLT QC as debugging, press CTRL+F6 to pop up the dialog
box of PLT adjustment. Enter 4444 to adjust PLT gain value, making the peak of PLT is
7.4-8.0. See figure 6-7.

Figure 6-7 PLT Gain Adjustment 2

6.3 Motor Test

Click ‘Service’ in software count interface and input ‘5555’ to enter motor test.

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 Chapter 6 Test

Figure 6-8 Motor Test

If motor parameter modification is needed, please input the value in the blank box at
the right side of corresponding parameter. Press ‘Enter’ to make your modification
succeed.
If motor test is needed, please input your value to the left side box and press ‘+’,
then the motor starts to work.

Figure 6-9 Parameters

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 Chapter 6 Test

6.4 Value Modification

Choose and double click the value in Test or Query interface to pop up the interface
shown as in Figure 6-10, and input new value in the chosen box.

Figure 6-10 Value Modification

6.5 Optical Debugging

Click ‘Service’, input ‘3333’ and press ‘Enter’ to go into optical debugging interface.
（See Figure 6-11）

Figure 6-11 Optical Debugging

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 Chapter 6 Test

NOTE: please take reference to Optical Module Installation & Debugging

(2014.09.01) for details.

6.6 Optical Debugging

Click Service, input 77770 and click OK to enter Calibration---Others. The P_LCR,
MON%, EOS% and BASO% can be calibrated here.

Figure 6-12 Others

6.7 Software Upgrade

Figure 6-13 Upgrading Software

Double-click to run the installation program, install the software to the path in Figure
6-14. In most cases, this default path is appropriate.（D：\Program Files\UT5380）

Figure 6-14 Installation Path

6.8 Software Restarting

Click ‘×’ in top right corner to pop up the dialog box shown in Figure 6-15, click ‘Exit’
the exit the program. Click software icon on your desktop to restart the software.

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 Chapter 6 Test

Figure 6-15 Software Restarting

6.9 Skip Self-checking

It’s usually needed to restart the computer, the analyzer and the software in
maintenance. Press ‘Ctrl+F12’ as the interface shown in Figure6-10 comes out to skip
self-checking.

Figure 6-16 Skip Self-checking

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 Chapter 7 Upgrade Process

7.1 Upgrade Process of Flow Program

7.1.1 Preparation

1、 Copy the flow process folder to the computer.

2、 Double click ‘HCL_MODEL’, see Figure 7-1.

Figure 7-1 Upgrading Software Icon

3、 Double click this icon and pop up the following interface. click ‘Download’.

Figure 7-2 Upgrading Interface

4、 Choose right serial, then the indicator on the right lights, or, it’s grey. （ See
Figure7-3）

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 Chapter 7 Upgrade Process

Right serial, indicator lights, or it’s grey

double click
upgrading
Input the ID of program
upgrading program
程序的 ID 号

Figure 7-3 Download Interface

5、 Click ‘Batch Download’ to upgrade all programs. Do remember to backup
parameters of ‘4444’ and ‘5555’.
6、 If upgrading signal program, click ‘Query’ to find it’s ID and then click ‘Cancel’
to exit. Double click the upgrading program, input found ID, click ‘Download’
and then restart the analyzer.

Files
文件名与 name
所 and
corresponding
对应的 ID 号 ID

Figure 7-4 ID Query Interface

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 Chapter 7 Upgrade Process

7.2 BIOS Upgrade

Double click and the dialog box （ see Figure7-5 ） pops up. Click
‘Configuration’ , the baud rate should be 115200, and the COM port should match with
the computer. Click ‘OK’ and exit.（See Figure7-6）

Figure 7-5 BIOS Upgrade

Figure 7-6 Baud Rate and Serial Selection

Choose ‘Connect’ in , and then choose ‘ASC mode’ in .

（See Figure7-7）

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 Chapter 7 Upgrade Process

Figure 7-7 BIOS Upgrade

Press ‘1’ to choose , and choose ‘Transmit’ in

（ see Figure7-8 ） to find ‘UT5380_BIOS’. Double click it and pop up the

following interface.
Double click ‘UT5380_BIOS’ to see Figure7-8.

Figure 7-8 Downloading

comes out as downloading finished . Pressing

‘N’ means no running. Input ‘2’ and choose ,
see following figure.

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 Chapter 7 Upgrade Process

Figure 7-9 BIOS Upgrade

Press ‘0’ to choose , input ‘Y’ to finish

upgrading（see Figure7-10）, exit and then restart the analyzer.

Figure 7-10 BIOS Upgrade

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 Chapter 8 Troubleshooting

8.1 Optical Fault

8.1.1 Stains on WOC Flow Cell

Wrong optical classification, it cannot be clearly classified the blood sample to 3 cell
populations. Please measure the 0°optical background voltage. Measurement method
please see Figure8-1.

Figure 8-1 Measurement method

Connect with multimeter and use DC, if the displayed voltage is within 1V, it’s
considered to be qualified. The background voltage may be a little bit high because of
stains on the lens. Remove the WOC flow cell so as not to irradiated by laser. （ See
Figure 8-2） If the voltage is over 300mV, for example, the voltage is 2.3V as laser going
through the WOC flow cell, and voltage is 800mV as moving the WOC flow cell away.
Therefore, the outer-wall or inner-wall of WOC flow cell is determined to be stained. Wipe
around with a clean cloth and place it back and check the voltage again. If it’s within
1.1V, which can be determined the outer-wall stained. If it has not obvious changes, it
may inner-wall stained. Open the green and black connectors of optical flow interface,
drain liquid in WOC flow cell via syringe inserted into green connector and inject probe

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 Chapter 8 Troubleshooting

detergent which flows out from black connector, soak it for a while and then do the
background voltage test till getting approximately same voltage.（See Figure 8-3）

Figure 8-2 Remove WOC Flow Cell

Figure 8-3 Soak Inner-wall of WOC Flow Cell

8.1.2 Stains on Image Forming Lens

Make optical background voltage test, if it’s pretty high ( 5.6V in multimeter and 5.4V
as moving the WOC flow cell away), it can be determined to be image forming mirror
stained. Remove the image forming mirror, screw down the socket head cap screws,
unscrew the clamping ring, take the lens out and wipe it. Do not unscrew the set screw
and adjusting nut. (see Figure 8-4)
Clean up the two lens and put them face to face (convex to convex). Then place lens
into lens barrel and tighten the clamping ring. Test the background voltage till it drops

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 Chapter 8 Troubleshooting

to 1V. As installing the image forming lens, please making it as close to the WOC flow
cell. The light spot falls onto the strip light bar which is behind the WOC, when laser
passing through the image forming lens. (See Figure 8-5) Fine tuning the mirror 1
level knob (lower left corner), multimeter voltage displays maximum value is better.
Fine tuning the mirror 2 level knob (top right corner), multimeter voltage displays
minimum value is better.

Figure 8-4 Image-forming Mirror

Figure8-5 WOC Flow Cell

8.2 Change WOC Flow Cell

When the WOC flow cell loosens or falls off, please open the front shell and the

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 Chapter 8 Troubleshooting

shield. If there’s liquid in the WOC, please change the WOC or bond it again. Unplug the
tubing of WOC flow cell, unscrew the fixing screw, move the WOC away and take it out.
Change a new WOC flow cell and make the WOC reflected light (the highlight) shining
into the laser transmit aperture. Make 3333 sample test in Service. Fine tuning 0° and
10° knobs to make cell test value maximum. (see Figure 8-6）

Figure 8-6 Total Number of Signal

Adjust the direction of 90 °, unplug 90 °, 90 ° D signal lines in the ADFIFO board, or
open PMT tube shield and irradiate vertically against WOC by the flashlight after turning
off the power. There will be two large black vertical lines onto the slit.（see Figure8-7）

Figure 8-7 Parallel Lines of Slit Straight Line

Irradiate towards left or right 15 ° angle, the straight lines become arcs, just like
"brackets" shape. These two arcs should be tangent.（see Figure 8-8）

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 Chapter 8 Troubleshooting

Figure 8-8 Brackets Projection

If not, please loosen the cut-nail of microscope and turn the knob, making them tangent.
（see Figure 8-9）

Figure 8-9 Magnifier

Adjust WOC 90 ° knob, making the slit being in the middle of straight lines.（see

Figure 8-10）

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 Chapter 8 Troubleshooting

Figure 8-10 Parallel Lines of Slit Straight Line

Cover the PMT tube shield, turn on the power and make sample test. Please take

reference with the Optical Module Installation to debug.

Not a good optical debugging:

Figure 8-11 Optical Debugging 1

Figure 8-12 Optical Debugging 2

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 Chapter 8 Troubleshooting

Figure 8-13 Optical Debugging 3

NOTE: there’s smear when testing old blood, which is normal. Cells shape changes and
form smear after placing in a long time.

55
【Total distribution and after-sales service】
URIT Medical Electronic Sales CO., LTD
Address：3 Fuhe alley, Zhong Hua Road, Guilin
P.C：541001
Tel：0773-2288555 2288558
Fax：0773-2288559 2824559
After-sales Service：0773-2288566 2825742
400 Hotline：400-727-2288
E-mail：[email protected]

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