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Experiment No.3

1) The document describes an experiment to observe plant cell structures using an onion bulb and Elodea leaf under a microscope. 2) Key structures observed in onion cells include the cell wall, cell membrane, cytoplasm, central vacuole, nucleus, and nucleolus when stained with iodine. Elodea cells contain cell walls, cell membranes, cytoplasm, nuclei, and many chloroplasts. 3) Cyclosis, the movement of chloroplasts around the cell periphery, was sometimes observed in Elodea cells and is caused by cytoplasmic streaming.

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Johnren Godinez Booc
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107 views

Experiment No.3

1) The document describes an experiment to observe plant cell structures using an onion bulb and Elodea leaf under a microscope. 2) Key structures observed in onion cells include the cell wall, cell membrane, cytoplasm, central vacuole, nucleus, and nucleolus when stained with iodine. Elodea cells contain cell walls, cell membranes, cytoplasm, nuclei, and many chloroplasts. 3) Cyclosis, the movement of chloroplasts around the cell periphery, was sometimes observed in Elodea cells and is caused by cytoplasmic streaming.

Uploaded by

Johnren Godinez Booc
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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Experiment No.

NAME: Johnren G. Booc DATE PERFORMED: 09/24,2022


GROUP NO: Group 7 DATE SUBMITTED:
SECTION: ST12A4 RATING:

Cell Structure (PLANT CELL)

INTRODUCTION
The invention of the microscope gave rise to the discovery of the cells. Microscopes are
tools that aid the naked eye in viewing microorganisms by enlarging the image so as to
be studied. In this lab, we will be using a compound light microscope and will learn
about its proper use and handling.

OBJECTIVES
·To prepare a wet mount of onion cells;
·To observe the different organelles of a plant cell.

MATERIALS
Glass slide
Cover slips
Elodea leaf
Microscope
Onion bulb
Water
Forceps
Methylene blue
Scalpel
PROCEDURE:
Part A.
Basic Plant Cell Structure (Onion Epidermis)

1.Obtain a piece of a single piece of sliced onion bulb. Snap the piece in half and, using forceps, peel
a bit of tissue-like, transparent epidermis from the inner layer. Mount the piece of epidermis in tap
water on a glass microscope slide. Be certain that it is flat and not doubled over itself. Add a coverslip
and gently press it down with a pencil eraser to remove as many air bubbles as possible.

2.Observe the specimen under low power. If you see a confusing mass of overlapping cell parts, you
probably do not have a good piece of epidermis. Scan your slide for a single layer of cells that
resembles a brick wall. If you cannot find such a view, then prepare another slide.
3.As you view onion epidermis cells, note that the cell membrane is tight against the cell wall and
too thin to be seen with a light microscope. Note also any signs of nuclei.
4.Remove the slide from the microscope. Add a drop of iodine stain to the slide immediately next to
the coverslip but not on top of it. With a small piece of paper towel, draw the stain under the
coverslip by placing the towel on the opposite side of the stain and touching the water. This action
should wick the water out from under the coverslip and draw the stain in.
5.Observe the epidermis cells again under low power. Note that the cells are now colored, with
the cell wall, nucleus and nucleoli stained more darkly than the cytoplasm. Try to distinguish
between the cytoplasm and the large central vacuole.
6.Observe the cells under high power to help you better distinguish cell structures.
7.Make a sketch of a few onion cells. Label the cell wall, cell membrane, cytoplasm, central
vacuole, nucleus and nucleolus.
8.Wash and dry the slide and coverslip for the next specimen.
DRAWING

Part A:
Onion

1. How do the cells of the onion compare with those of the cells you have seen before?

2. How did the stain affect what you observed?

3. What is the reason behind the size of a plant cell?


Eventually, cells, including both animals and plants, should really match up together just like puzzle pieces to
shape tissues and organs. That also means that a cell's size should be proportional to its position within the tissue
as a whole.
Part B.
Basic Plant Cell Structure (Elodea)

1.Break off a single leaf near the tip of an Elodea plant and prepare a wet mount.
Position the slide so that you are observing the edge of the leaf near the tip. Observe the cells both
under low power and high power. If you are viewing at the edge of the leaf you should see only one
cell thickness. You should also see two types of cells. One type of cell has a smooth margin facing the
environment; the second type, called a spike cell, has a projection out into the environment.

2.The thick cell walls and the green chloroplasts are the most conspicuous structures. Sometimes a
shadow of a nucleus can be seen. If you are patient and fortunate, you may see movement of the
chloroplasts around the periphery of the cell. This movement, called cyclosis, is caused by
cytoplasmic streaming.
3. Make a labeled drawing of a few Elodea cells to demonstrate your observations. Labe the cell
wall, cell membrane, cytoplasm, nucleus and chloroplast.
DRAWING
Part B:
Elodea

1.What is the general shape of an Elodea cell?


Rectangular shape.

2.What do you think the purpose of cyclosis is?


Cytoplasmic streaming, further known as a protoplasmic streaming and cyclosis, is indeed the motion of
cytoplasm within the cell caused by cytoskeleton forces. Its purpose is still most likely to boost the movement of
molecules and organelles around the cell.

CONCLUSION:
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