Which type of microscopy converts ➢ Stage clip

differences in refractive index into

Which of the following is a unique feature
variations in light intensity to obtain the
of the gram-negative bacterial cell wall
specimen image?
that separates the inner membrane from
➢ Phase-Contrast the outer membrane?
Type of microscopy that produces a ➢ Periplasmic space
magnified image that appears dark
It is an instrument that used to sterilize
against a bright or white background.
glassware in microbiology laboratory that
➢ Brightfield uses steam under pressure.
Which is not true regarding Ziehl-Neelsen ➢ Autoclave
Method?
Which of the following structures is utilized
➢ Uses malachite green as by the bacteria in exchanging genetic
counterstain material from one bacterium to another?
It enhances the resolution of the ➢ Pilus
microscope and is used to fill the space
In what situations should you tie your hair
between the objective lens and the glass
in the laboratory?
slide.
➢ All the time
➢ Cedarwood Oil
What is the most commonly used light
Which type of microscope can visualize
source for brightfield microscopy?
objects that are smaller than 0.2um using
a 100,000x magnification? ➢ Incandescent and Tungsten
Halogen
➢ Electron microscope
What does a dash symbol engraved on
Part of the endospore that causes it to be
the objective lens of the microscope
heat-resistant.
indicate?
➢ Dipicolinic acid
➢ Do not use a coverglass
Student A accidentally spilled
At what temperature are microbiological
hydrochloric acid in his hand. What
incubators set at?
should he first do?
➢ 35C deg and 37C deg
➢ Flush the area with large amount of
water for 15 minutes. What is the initial magnification of the
specimen under observation if the
Type of precaution that did not apply to
microscopist is using the high-power
feces, nasal secretions, sputum, sweat,
objective?
tears, urine, or vomitus unless
contaminated by visible blood. ➢ 40x
➢ Universal Precautions Which among the bacterial structure acts
as the functional analogue for the
The mechanical stage is the part of the
eukaryotic nucleus?
microscope where the specimen is
placed. What hold the glass slide in ➢ Chromosome
position?
Which type of electron microscope What is the working distance between
allows the visualization of the internal the low power objective and the
structures of cells? coverslip of the histologic slide?
➢ Transmission electron microscope  7.5 mm
Clara was rushed to the clinic when the You are given a new slide in the
solution splashed on her face. She did not laboratory. Which part of the microscope
follow instructions and added distilled will you use to put it in general focus?
water to HNO3. What might be the cause
➢ Coarse adjustment knob
of the sudden splashing?
It mathematically expresses the resolving
➢ Rapid generation of heat upon
power of the objective.
mixture of chemicals.
➢ Numerical aperture
What is the size of the coverslip slip used
in microscopic examination of Which of the following glassware in the
microbiologic specimen. laboratory is used to hold liquids in the
preparation of culture media?
 24mm X 24mm
 Test tube
It pertains to the ability of the objective
lenses to retain the same central field of Which of the following is/are used to
view when the user switches from one demonstrate gas production by
objective to another. fermentative bacteria.
➢ Parfocal ➢ Durham Tube
It is a component of a monocular or Which of the following is not a personal
binocular microscope where the protective equipment?
intermediate image is formed.
➢ Laboratory Cover
 Objectives
Bacterial flagellar arrangement emerging
In which microscope can Treponema from the entire cell surface.
pallidum be visibly observed?
➢ Peritrichous
➢ Darkfield microscope
Which of the following makes the
How do you differentiate prokaryote from enzymes that break down large
eukaryote? molecules into smaller, easy-to-transport
molecules?
➢ The cell lacks a nucleus
➢ Cytoplasm
It is a conventional device that is used for
chemical reactions that required It is known as the ability of the lens to
controlled environment at a constant enhance parts within a cell.
temperature.
 Contrast
➢ Water bath ➢ Contrast?
Magnifying glass is an example of what Part of the microscope that is used to
type of microscope? adjust the lateral separation of eyepieces
for each individual
➢ Brightfield
➢ Interpupillary control
The infectious agent must have a way to ➢ 15 MINUTES
exit the reservoir to continue the chain of
It is the preferred specimen for an acid-
infection. What is not done to break the
fast bacilli microscopy.
chain of infection?
➢ FIRST MORNING SPUTUM
 Sealed specimen containers
These are practices or procedures
This is used to inoculate liquid biological
performed to prevent contamination of
samples such as urine in an agar-based
specimen from pathogen prior to
culture media.
aspecimen collection.
➢ Inoculating Loop
➢ ASEPTIC TECHNIQUE
An instrument or glassware that hold
STAT means
microscopic slides simultaneously for most
efficient staining processing. ➢ IMMEDIATELY
➢ Staining Rack Ideally, specimens should be collected
during the acute phases of an illness.
A procedure that uses a needle to
aspirate specimen above the pubic ➢ True
symphysis through the abdominal wall
If possible, specimens should be
into the full bladder.
collected after antibiotics are
➢ SUPRAPUBIC ASPIRATION administered.
A procedure that is known as puncturing ➢ False
of the eardrum.
Stool specimens cannot be collected
➢ MYRINGOTOMY from clean, non-sterile containers.
It is the specimen of choice for the ➢ False
detection of gastrointestinal pathogens.
It should never be assumed that the
➢ STOOL/FECES/FECAL SAMPLE patient knows how to collect a specimen.
It is the required volume of CSF to be ➢ True
collected for microbiologic and chemical
Swabbing from pustule or ulcer enhances
testing.
the recovery of anaerobic bacteria.
➢ 10ML
➢ False
It is the recommended anticoagulant for
Swabs are generally excellent specimens
blood culture.
compared with tissues or needle
➢ SODIUM POLYANETHOL SULFONATE aspirates.
It is the specimen of choice for the ➢ False
isolation of SARS-COV 2 for RT-PCR.
For lesions, the sample is collected from
➢ NASOPHARYNGEAL SWAB the advancing margin, preferably by
needle aspiration.
Within how many minutes should CSF
samples be immediately transported to ➢ True
the laboratory to ensure the reliability of
results?
Dacron swabs may have excessive fatty
acids which are inhibitory and toxic to
some bacteria.
➢ False
No more than three sets of blood culture
should be drawn in a 24-hour period.
➢ True
Aseptic technique prior to specimen
collection hasten the multiplication of
contaminants in the specimen
➢ False