Effect of Heat On Vitamin C in Tomatoes
Effect of Heat On Vitamin C in Tomatoes
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Abstract
Objectives/Goals
Vitamin C is an important vitamin, and tomatoes are a good source of Vitamin
C. Most tomato products we consume have been processed by heat, and I wanted
to discover if cooking altered the level of Vitamin C. My goal was to determine
if heating the tomatoes to three different temperatures affects the level of
Vitamin C. My hypothesis is that the Vitamin C content of tomatoes will
decrease when heated because Vitamin C is water soluble and is affected by
heat.
Methods/Materials
I used titration to test my hypothesis. The Vitamin C in the tomatoes is the
titrant, and iodine is the titrating solution. I made a tomato solution by
blending store-bought red tomatoes with 200mL of distilled water. I filtered the
solution to remove seeds. A 10mL sample was removed and set aside as the
control. The Vitamin C from this sample is the dependent variable. The
remaining tomato solution was heated on a gas stove, and three 10mL samples
were taken at three different temperatures, 50C, 75C, and 98 C (independent
variable). While the samples cooled to a standard temperature of 17C, I
prepared a starch solution (1T cornstarch and 200mL distilled water). Ten
drops of the starch solution were added to each of the four samples. I then
added the titrating solution, iodine (Iodine Tincture USP) one drop at a time to
the samples and recorded the number of drops necessary to change the pink
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Results
The presence of Vitamin C in a fresh tomato solution declined after it was
heated. In the first trial, my uncooked sample needed five drops of iodine to
change color, while the other three samples needed 20% less solution (four
drops). Trial Two started with six drops for the Control and concluded with four
drops for the 98C sample. The final trial gave the clearest results--the Control
required seven iodine drops to change to blue/black and the 98C sample only
required four drops (43% less).
Conclusions/Discussion
This experiment showed that Vitamin C in a tomato can decrease due to heat.
Heat causes the Vitamin C content in tomatoes to decrease by decomposing the
water-soluble vitamin. Tomatoes that are cooked will have less Vitamin C than
raw tomatoes. If you want the most Vitamin C from a tomato--EAT
FRESH!
Summary Statement
This project showed that Vitamin C in raw red tomatoes is reduced by heat.
Help Received
My science teacher, Mr. Jeff Fox, loaned me laboratory equipment. My mom
was my laboratory assistant.
Bibliography
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