Code No: R05222302 Set No.

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II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. (a) Define the terms qualitative & quantitative analysis.

(b) Differentiate between micro & semi micro analytical methods.
(c) What are advantages & limitations of chemical methods. [6+6+4]

2. Compare & contrast the techniques phase -contrast microscopy & differential in-
terference contrast microscopy (DIC) [16]

3. Explain the following terms:

(a) Gravitational force.

(b) Centrifugal force
(c) Sedimentation coefficient. [5+5+6]

4. You have two samples of DNA derived from different organisms,A & B. DNA from
A shows an increase in optical density begnning at 700 C & plateau at 800 C. DNA
from B shows a corresponding increase in O.D beginning at 650 C & plateau out
at 760 C. What is your conclusion. Justify your answer. [16]

5. (a) Define fluorescence. Derive an expression relating intensity of fluorescence &

concentration.
(b) Write a brief note on :
i. Triplet state
ii. Doublet state
iii. Singlet state. [8 × 2 = 16]

6. (a) Discuss the basic principle of infra red spectroscopy?

(b) Briefly describe the scanning of infrared spectrum of an organic compound.
[8 × 2 = 16]

7. Explain the following :

(a) Free inductive decay

(b) Hetero nuclear decoupling
(c) Off resonance coupling
(d) Chemical shift. [8 × 2 = 16]

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Code No: R05222302 Set No. 1
8. Give an account on electronic structure & hyperfine splitting in ESR spectroscopy?
[16]

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Code No: R05222302 Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Give a brief account on methods for reporting analytical data? [16]

2. Compare & Contrast the optical magnification obtained by the bright & dark field
microscopy along with their specific applications. [16]

3. (a) Give the basic principle involved in analytical centrifugation.

(b) What are the important application of this technique in biochemistry?
[8 × 2 = 16]

4. You have two samples of DNA derived from different organisms,A & B. DNA from
A shows an increase in optical density begnning at 700 C & plateau at 800 C. DNA
from B shows a corresponding increase in O.D beginning at 650 C & plateau out
at 760 C. What is your conclusion. Justify your answer. [16]

5. (a) Define optical activity? What is the principle involved in circular dichroism
spectroscopy?
(b) Differentiate the polarimetry & circular dichroism. [8 × 2 = 16]

6. Discuss the principle, importance & applications of mass spectrometry? [16]

7. (a) Give the principle underlaying in NMR spectrometry?

(b) Describe the component of a NMR spectrometer with a neat labeled diagram.
[8 × 2 = 16]

8. (a) What is the principle of ESR. Discuss some important applications of ESR.
(b) What are the limitations of ESR. [8 × 2 = 16]

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Code No: R05222302 Set No. 3
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Give an account of the instruments involved in electrochemical methods of analysis

along with their principle of working. [16]

2. (a) Give the basic principle involved in the viewing an object in dark field.
(b) Draw a neat labeled diagram of optical pathway of dark field microscope.
(c) Give its specific use. [5+6+5]

3. (a) Give the basic principle involved in analytical centrifugation.

(b) What are the important application of this technique in biochemistry?
[8 × 2 = 16]

4. What are the different regions of the electromagnetic spectrum & where are they
used for? [16]

5. Define the following terms:

(a) chiral compounds

(b) cotton effect
(c) circular birefringence. [6+5+5]

6. How will you distinguish between intermolecular & intramolecular hydrogen bond-
ing by IR spectroscopy? [16]

7. (a) Explain the principle involved in CMR spectra?

(b) Why it is not possible to determine relative ratio of carbon atoms in a com-
pound by integration of peak areas in 13C NMR as in PMR?. [8 × 2 = 16]

8. Why ESR spectroscopy is widely used in the study of chemical, photochemical &
electrochemical reactions? Justify your answer. [16]

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Code No: R05222302 Set No. 4
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write an account on analytical methods based on the physical property measure-

ment? [16]

2. Narrate the principle of operation of a fluorescence micrscope. Give its applications

in biology? [5+11]

3. Calculate the molecular mass of the protein where the sedimentation coefficient of
the protein is 7.75 ×10−13 s. In subsequent analysis the protein was found to have
an average diffusion coefficient 0f 4.0 × 10−11 m2 s−1 & the a partial specific volume
of 0. 734 ×10−3 m3 kg −1 .

(a) convert the molecular mass to relative molecular mass.

(b) Express the molecular mass in Daltons. [8 × 2 = 16]

4. Define the terms:

(a) i. Radiant power( P )

ii. Path length of radiation
iii. absorptivity
iv. molar absorptivity.
(b) Describe & derive the Beer’s law.
(c) What are its limitations & deviations. [4+6+6]

5. (a) What is atomic absorption. What are its advantages & disadvantages.
(b) Give some of its limitations. [8 × 2 = 16]

6. (a) What are the different regions of infrared radiation?

(b) Explain various types of stretching & bending vibrations with suitable exam-
ples. [8 × 2 = 16]

7. (a) Why does a signal for a particular set of protons is split into a multiplet.
(b) explain with suitable examples the trem spin-spin coupling [8 × 2 = 16]

8. (a) Which of the following two formulas is correct for the complex obtained be-
tween copper ion & 8 quinolinethanol (C9 H7 N5 )
i. Cu(I) (C9 H7 N5 )(C9 H6 N5 )
ii. Cu(II) (C9 H6 N5 )2

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Code No: R05222302 Set No. 4
show that the ESR approach is appropriate for this determination.
(b) Which is one of the best known free radicals used in calibrating ESR spectra?
[8 × 2=16]

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