Cytogenetics Practice Test 1 Pre-Finals

Total points 50/50

Good day guys! this is a practice test for Cytogenetics to test your knowledge for the
upcoming 

Pre-Finals  exam. Please be advised that this test may or may not reflect  on the 

Pre-Finals  exam. Also, this is not compulsory and it only serves as a brain exercise,
however, I am hopeful that this will help you remember the basic concepts on
Cytogenetics. Kindly answer as honest as possible. Good luck!

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Introduction to Chromosomes 38 of 38 points

Read the questions carefully and please answer as honestly as you can, good luck!

Eukaryotic chromosomes are sequestered within a __.

* 1/1

nucleoid

chloroplasts

nucleus
 How are you going to use each of these tests to measure & detect Transcription? *

`RNase
rotection
DNA In situ MS2 Northern RNA-
ssay and RT-PCR Score
microarrays hybridization tagging blot Seq
ChIP-Chip
of RNAP

by
incorporating
RNA stem
loops into a
1/1
gene, these
become
incorporated
into new RNA.

the traditional
& the most
quantitative
method before 1/1
the advent of
more modern
test

measures the
relative
abundance of
1/1
the global total
or nuclear RNA
levels

detect active
transcription 1/1
sites

detects the
presence of a 1/1
transcript

measures the
absolute
abundance of 1/1
total or nuclear
RNA levels

applies next-
generation
sequencing
techniques to 1/1
sequence
whole
transcriptomes,

measures the
relative
abundance of 1/1
newly formed
transcripts
It divides the chromosome into a short arm (p) and a long arm (q):
* 1/1

Chromatid

Plasmid

Centromere

Telomere

Cytogeneticists use these three things to tell chromosomes apart *1/1

EXCEPT:

chromosome size

the color of the telomere

characteristic banding patterns

the position of the centromere

Identify the parts of this actual chromosome.
*

A B C D E Score

p arm 1/1

centromere 1/1

q arm 1/1

q tel 1/1

p tel 1/1
Chromosome Dyes
*

labeling specific for

binds to the
chromosome- the
adenine-
specific DNA phosphate Score
thymine-rich
with groups of
regions
fluorophores DNA

Giemsa 1/1

Quinacrine 1/1

Total number of human autosomes.

* 1/1

46

44

23

22
Classify these actual chomosomes according to the location of their centromeres
*

A B C Score

Metacentric 2/2

Submetacentric 2/2

Acrocentric 2/2

Which of the following is not an extranuclear DNA?

* 1/1

plasmids

mitochondria

chloroplasts

nucleoid
Bacterial chromosomes have these characterististics EXCEPT: * 1/1

Located in NUCLEOID

Folded into loops that are 50,000-100,000 bp

Usually one chromosome

Linear rather than circular

iDENTIFY the pairs of the following chromosomes
* 2/2

Option 2 Option 4

Option 5 Option 3
 Option 1

Methods of Karyotyping
*

quantify the
uses a dye to visualize all
DNA copy
stain chromosomes
number on a Score
chromosome pairs in
genomic
bands different colors.
scale

Classic
1/1
karyotyping

Spectral
1/1
karyotyping

Digital
1/1
karyotyping

Chromosomes were first observed in tumor cells by ___ in 1882.

* 1/1

Thomas Hunt Morgan

Gregor Johann Mendel

Walther Fleming

Which of these organisms has only 1 chromosome?

* 1/1

Escherichia coli

1st & 3rd choices only

Agrobacterium tumefaciens

2nd & 3rd choices only

Vibrio cholerae

all of the above

 Functions of Plasmids
*

carry genes
for being
carry genes less
carry genes conjugation,
for susceptible
for transfer of Score
proteinaceous to
pathogenicity genes
toxins antimicrobial
drugs or
toxins.

Resistance
1/1
(R) factors

Virulence
1/1
plasmids

Fertility (F)
1/1
factors

Bacteriocin
1/1
factors

Introduction to Chromosomes 12 of 12 points

Read the questions carefully and please answer as honestly as you can, good luck!

Moral lesson  of Introductory  illustration: * 2/2

vengeance belongs to man

any of the above

vengeance belongs to divine

In karyotyping, which features differentiate CHROMOSOME NO. 1 & 2 *2/2
from the other chromosomes?

longest among chromosomes

having the most number of bands

more than one telomeres

larger centromeres

3rd & 4th choices only

1st & 2nd choices only

all of the above

In this type of karyotyping,  chromosomes are visualized in different *2/2

colors:

Spectral

Classic

Digital
Identify the parts of Chromosome:
*

centromere q-arm telomere Score

A 2/2

B 2/2

Chromosomes in prokaryotes are _____.

* 2/2

usually single molecule of DNA

linear rather than circular

located in nucleus

all of the above

first 2 choices

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