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Biochem Lab Exercise No. 1 2

This document summarizes the results of two laboratory exercises on water properties and pH/buffers. In Exercise 1, students observed and recorded the results of diffusion, dialysis, and osmosis experiments. They also tested water's ability to dissolve various substances. In Exercise 2, students measured the pH of solutions before and after adding acid or base to determine which contained buffers. The review questions ask about water's role as a solvent, definitions of diffusion, osmosis, and dialysis, and biological buffering systems.

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EMMANUEL CHARLES ADRIAN BAUTISTA
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45 views

Biochem Lab Exercise No. 1 2

This document summarizes the results of two laboratory exercises on water properties and pH/buffers. In Exercise 1, students observed and recorded the results of diffusion, dialysis, and osmosis experiments. They also tested water's ability to dissolve various substances. In Exercise 2, students measured the pH of solutions before and after adding acid or base to determine which contained buffers. The review questions ask about water's role as a solvent, definitions of diffusion, osmosis, and dialysis, and biological buffering systems.

Uploaded by

EMMANUEL CHARLES ADRIAN BAUTISTA
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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EXERCISE NO.

WATER & ITS PROPERTIES

REPORT SHEET

GROUP #: DATE PERFORMED:

NAMES: COURSE/YEAR:

I.ILLUSTRATION

Draw and label the following setup:

1. Diffusion
2. Dialysis
3. Osmosis
Continuation of illustrations....
II.RESULT AND DISCUSSIONS

1. Complete the table ( Part A: Water as a Universal Solvent)

Substance Solubility in Water Solubility in CCI4

NaCI
Sugar
Gelatin
CuSO4
Cooking oil
Ethanol

2. What conclusions can you make regarding the solubility of substances to water and CCI 4
based on the obtained results?

3. What happens when powdered acid and powdered sodium bicarbonate are mixed together?
What happens when is added to the mixture?

4. Describe the appearance of water solution right after the addition of potassium
permanganate and the appearance of solution after 30 minutes.
5. Describe the appearance of the water-iodine solution in the beaker and the starch
Solution inside the water cellophane bag right after the immersion and 30 minutes
after immersion in water-iodine solution.

6. Based on your observation in #5, describe the movement of iodine and starch about
the water cellophane bag.

7. Complete the table (Part C: Osmosis)

Weight of water cellophane bag before grams


immersion
Weight of water cellophane bag after grams
immersion
Change in weight of water cellophane bag grams

8. Based on your observation in #7, describe the movement of water in the water
cellophane bag.
REVIEW QUESTIONS:

1. Why is water able to dissolve many biological substances?

2. What is diffusion? Describe the different types of diffusion that occur in living systems.

3. What is osmosis? How does tonicity influence osmosis?

4. What is dialysis? Describe the movement of molecules in a dialysis setup.


5. Give some medical applications of dialysis

6. What are the other functions of water in biological system?


EXERCISE NO. 2

pH AND BUFFERS

REPORT SHEET

GROUP #: DATE PERFORMED:

NAME/S: COURSE/YEAR:

I.RESULTS AND DISCUSSION

pH before pH after
Tube No. addition of HCI addition of HCI Change in pH
or NaOH or NaOH
1
2
3
4
5
6
7
8

1. Based on the obtained results, which tubes contain buffered solution? Explain
Why you think so.

2. Which tubes contain unbuffered solution? Explain.


sREVIEW QUESTIONS:

1. Explain how buffer solution resist pH change?

2. Enumerate and describe the different biological buffering systems.

3. What is the most important biological buffer of the blood? Explain.


4. Why does the pH change by one unit if hydrogen ion concentration changes by a factor
of 10?

5. Explain the following terms in relation to ph & buffers:

a. Acid dissociation constant

b. Amphitathic

c. Buffering capacity

d. Equivalence point

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