KR-30

Promois KR-30 is hydrolyzed keratin, which is made from wool as the source and shows a faint odor and a low hue
characters, suitable as a raw material for hair-care cosmetics. The protein shows a low odor and a coloring change as
the progress of time and a high stability in a redox-agents-existing environment.
The protein can also show a high functional character with a synergy effect with cationic components in cosmetic
formulae.

Texure and Effect of Promois KR-30

・Promois KR-30 is a high-molecular-weight hydrolyzed keratin with a number-average molecular
weight of approximately 30,000, can show a good film-forming ability.
・Promois KR-30 film can cover damaged hairs and provide an adequate tension, a suitable stiffness,
a beautiful hair gross, and a gentle hair-gathering effect to hairs.

● The structure of Promois KR-30 ● A beautiful film made from Promois KR-30

H ( NH CH CO )x ( NH CH CO )y ( NH CH CO )z OH
(CH2 )n CH2 R
- + - +
COO Na SO3 Na
x, y, z = the polymerization degree of amino acids
R = the side chain of amino acid
n = 1 or 2
The appearance of the air-dried film of Promois KR-30

● Molecular illustration ● Schematic illustration

Promois KR-30 Conventional hydrolyzed keratin

Promois KR-30

● Film forming ability

The section view
Hair surface of the hair hair

Conventional hydrolyzed keratin

After being treated with Promois KR-30 of which N-terminal was labeled with
rhodamine B (PhlTC), the damaged hair was observed with a fluorescence
microscope.

● Sensory character ● Luster providing ability

Luster Promois KR-30

15
Water
10

5 Treatment method
Tress were sprayed
Manageability 0 Tension with an aqueous
and Promois KR-30
stiffness solution (the solid
component: 0.5%)
and water,
respectively.

Smoothness
Treatment method Evaluation method
One half and the other half scalps of panel were The evaluation was performed by 10 panels.
sprayed with an aqueous Promois KR-30 solution Grading, good: +2 points, not good: 0 point, no
(the solid component: 0.5%) and water, difference: +1 point Water Promois KR-30
respectively. (The evaluation was done by the comparison of
both half scalps.)

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 Heat damage -inhibition
Promois KR-30 can suppress the decomposition of cystine and the generation of lanthionine
and damage of hair surface which is occurred by heat treatment.

● Generation of lanthionine

C O C O C O C O C O C O
HC CH2 S S CH2 CH C CH2 S S CH2 CH HC CH2 S CH2 CH
NH NH NH NH NH NH
Cystine Lanthionine
Dehydroalanine intermediate

● Generation of lanthionine -inhibitoin

1/2 Cystine Lanthionine

16.5 0.40
16.0 0.35
1/2 Cystine (mol%)

15.5 0.30

Lanthionine (mol%)
15.0
0.25
14.5
0.20
14.0
0.15
13.5
0.10
13.0
12.5 0.05
∼
∼
0 0.00
Untreated Control Promois Untreated Control Promois
KR-30 KR-30
Experimental method
After being soaked in either a.m. 1% Promois KR-30 or water for 1 min, hair tress was treated 5 times by a 180℃ hair-iron.
After the treatment procedure was repeated 30 times, the tress was washed with 25% Sodium Laureth Sulfate (SLES) solution, and the amino acid composition of the
hair was analyzed.

● Damage of hair surface -inhibitoin

Control + Heat treatment Untreated Promois KR-30 + Heat treatment

Experimental method
After being soaked in either a.m. 1% Promois KR-30 or water for 1 min, permed hair tress was treated 5 times by a 180℃ hair-iron and washed with 25% Sodium
Laureth Sulfate (SLES) solution. After the treatment was repeated 30 times, hair surface was observed by a scanning electron microscope.

Synergy effect with cationic components

Promois KR-30 can show an excellent sensory character with a synergy effect with cationic
components in cosmetic formulae.
After being treated with Promois
● Imaginable illustration ● Sensory character KR-30 (the solid component:
1%) or water at 40 ℃ for 10 min,
Hair tension and stiffness the hair tress was washed with
running water.
20
15
10 After being treated with
stearyltrimethylammoium chloride
Favorability 5 Moist-feel at 40 ℃ for 10 min, both hair
tresses were washed with running
0 water and dried.

After three cycle of the process

described above was done, the
sensory evaluation of the hair
tresses was performed.
Hair-gathering effect smoothness
The evaluation was performed by 10
Water panels.
Grading, good: +2 points, not good: 0
Promois KR-30
point, no difference: +1 point
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 Permanent waving agent
・Promois KR-30 has a clear benefit which can allow cosmetic chemists to use it in permanent-wave
agent with low possibilities of the generation of odor and the appearances of coloring change.
・This permanent waving agent gives smoothness and softness to hair.

Reducing agent (w/v%) Oxidizing agent (w/v%)

Promois KR-30 3.00 Promois KR-30 3.00

Ammonium Thioglycolate 1.00 Sodium Bromate 7.00

Ethanolamine 1.84 Cocamidopropyl

0.20
Betaine(30%)
Cysteine 3.50
Disodium Phosphate 0.50
Cocamidopropyl
2.00
Betaine(30%) Phosphoric Acid 0.007

Pentasodium Disodium EDTA 0.10

0.15
Pentetate(40%)
Methylchloroisothiazolinone 0.01
Immediately At 25℃ Ammonium Hydroxide(25%) 1.00 Immediately At 25℃
after the for 3 months after the for 3 months Water up to
preparation Water up to preparation 100
100

Reducing agent (w/v%)

Oxidizing agent (w/v%)

Promois KR-
Control
30
Hydroxyethylcellulose 0.20 Promois KR-30 1.00 ―
Ammonium Thioglycolate (50%) 2.00 Sodium Bromate 7.00 7.00
Ethanolamine (80%) 2.30 Cocamidopropyl Betaine 0.20 0.20
Cysteine 3.50
Disodium Phosphate 0.50 0.50
Polyquaternium-39* 2.00
Phosphoric Acid 0.007 0.007
Cocamidopropyl Betaine 2.00
Polysorbate 80 0.10 0.10
Pentasodium Pentetate 0.15
Perfume 0.02 0.02
Cocamidopropyl Betaine / Cocamine Oxide / Oleth-10 3.00
/ Oleth-20 / Orange Yu / Glycerin / Water Disodium EDTA 0.50 0.50

Polysorbate 80 0.20 Methylchloroisothiazolinone ・ 0.01 0.01

Methylisothiazolinone
Perfume 0.10
Water up to 100 up to 100
Ammonium Hydroxide (28%) 1.00
pH6.0-7.0
Water up to 100
pH9.0∼9.5

● Sensory character ● Smoothness

Control
0.15
Promois KR-30
Smoothness
0.14
20
Friction coefficient

15 0.13
10
Favorability Moist-feel 0.12
5
0 0.11

0.10
∼
∼
0 Control Promois
Silky touch Softness KR-30

Treatment method Treatment method

reducing agent（25℃・15min)→rinse→towel dry reducing agent（25℃・15min)→rinse→towel dry
→oxidizing agent（25℃・15min)→rinse→dry→shampoo and rinse→dry →oxidizing agent（25℃・15min)→rinse→dry→shampoo and rinse→dry
Evaluation method Evaluation method
The sensory evaluation was performed by 10 panels. The averaged frictional coefficient of hair surface is measured with
The panels gave 2 points for the best, 1 point for the second best, and 0 point for FRICTION TESTER (KES-SE(STP))(Kato tech, Kyoto, Japan)
the poorest, and the sum of the points became the total evaluated value.

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 Amino acid composition

Amino acid mol% Amino acid mol%

Glycine 7.1 Proline 3.7
Alanine 6.8 Methionine 0.5
Serine 8.2 1/2 Cystine 0.6
Threonine 4.3 Cysteic acid 6.9
Valine 5.1 Aspartic acid 9.6
Leucine 11.0 Glutamic acid 16.7
Isoleucine 2.9 Arginine 6.8
Phenylalanine 2.9 Histidine 0.9
Tyrosine 2.4 Lysine 3.6

Product information

Composition 10% water solution of Hydrolyzed Keratin

*This product contains 1% Phenoxyethanol and 3% Butylene Glycol
as preservatives.

INCI Name Hydrolyzed Keratin、 Water

Raw material Wool

Average molecular 30,000

weight

Package 16kg case

Safety Data

Test item Result

Skin Sensitization (RIPT) Non-sensitizer

Ocular Irritation (HET-CAM) Non-irritant

Genotoxicity (AMES) Non-mutagenic

Human Patch Non-irritant

Data appear on this leaflet are actual obtained values by our experimental procedures, but not the specification values. Upon the use of the material on this leaflet, please
verify the functions and effects of the materials in your company’s using conditions. SEIWA KASEI cannot guarantee that the applications, which are introduced in this leaflet,
never interfere with any patents.
For certain reasons, the information of this leaflet may be changed without notice.

1-2-14, Nunoichi-cho, Higashiosaka, Osaka, Japan TEL +81-72-987-2626 FAX +81-72-987-2072

2011.2.28 Third Edition

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