MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.

SYLLABUS & SCHEME OF EXAMINATION

BACHELOR IN MEDICAL LAB

TECHNOLOGY (BMLT)

मेडिकल लैब टे क्नोलॉजी स्नातक पाठ्यक्रम

MADHYAPRADESH MEDICAL SCIENCES UNIVERSITY,
JABALPUR (M.P)

(Session 2015-17 & Onwards)

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

BACHELOR IN MEDICAL LAB TECHNOLOGY (BMLT)

SCHEME Of EXAMINATION:BMLT-1st Year
Theory Internal Practical Total
S.No. Subject
Assessment
Basic Histology 100 100 100 300
1 (Anatomy &
Physiology)
2 Microbiology-I 100 100 100 300
3 Biochemistry-I 100 100 100 300
4 Hematology-I 100 100 100 300
Total Max. Marks 1200
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively
for passing the examination and in practical he/she should get 50% marks i.e.-50
marks to get pass.
SCHEME Of EXAMINATION:BMLT-2nd Year
Theory Internal Practical Total
S.No. Subject
Assessment
1 Histology 100 100 100 300
2 Microbiology-II 100 100 100 300
3 Biochemistry-II 100 100 100 300
4 Hematology-II 100 100 100 300
Total Max. Marks 1200
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively
for passing the examination and in practical he/she should get 50% marks i.e.-50
marks to get pass.
SCHEME Of EXAMINATION:BMLT-3rd Year
Theory Internal Practical Total
S.No. Subject
Assessment
Applied 100 100 100 300
1
Histopathology
2 Microbiology-III 100 100 100 300
3 Biochemistry-III 100 100 100 300
4 Hematology-III 100 100 100 300
Total 1200
5 Instrumentation # -- 50 -- 50
Total Max. Marks 1250
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively
for passing the examination and in practical he/she should get 50% marks i.e.-50
marks to get pass.

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

-All theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of Examination
(Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Total

Question Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300
words
2 essay type Questions 15 30
Answer to be given in 450-500
words
Total Marks 100

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MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SYLLABUS & SCHEME OF

EXAMINATION FOR BMLT
FIRST YEAR

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

BMLT SYLLABUS

SR. NO. SUBJECTS PAGE NO.

1. BIOCHEMISTRY 03

2. HISTOTECHNOLOGY 04

3. ANATOMY PHYSIOLOGY 05

4. HEMATOLOGY 06

5. MICROBIOLOGY 07

6. VIROLOGY 08

7. PARASITOLOGY 09 - 10

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

BACHELOR IN MEDICAL LAB TECHNOLOGY (BMLT)

SCHEME Of EXAMINATION:BMLT-1st Year
Theory Internal Practical Total
S.No. Subject
Assessment
Basic Histology 100 100 100 300
1 (Anatomy &
Physiology)
2 Microbiology-I 100 100 100 300
3 Biochemistry-I 100 100 100 300
4 Hematology-I 100 100 100 300
Total Max. Marks 1200
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively
for passing the examination and in practical he/she should get 50% marks i.e.-50
marks to get pass.

-All theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of Examination
(Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Total

Question Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300
words
2 essay type Questions 15 30
Answer to be given in 450-500
words
Total Marks 100

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & Syllabus :BMLT-1st Year

Paper-I: Basic Histology (Anatomy & Physiology)

Theory Internal Practical Total

Subject
Assessment
Basic Histology 100 100 100 300
(Anatomy & Physiology)
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively for
passing the examination and in practical he/she should get 50% marks i.e.-50 marks
to get pass.
There shall be one paper setter external or internal for theory examination and two
examiners, one internal (Chairman) and one external for practical examinations.
Recognized teachers in Anatomy/Physiology after M.S.-Anatomy/MD-Physiology
with five years of teaching experience shall be on the panel of examiner.
- Theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of
Examination (Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Question Total Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300 words
2 essay type Questions 15 30
Answer to be given in 450-500 words
Total Marks 100
INSTRUCTION FOR THE PAPER SETTER

Section-A: This will consist of 10 very short answer type questions with answer to each
question upto five lines (Fifty to sixty words) in length . All questions will be compulsory to
answer. Each question will carry two marks. Total weightage of the section shall be 20 marks.
Section-B: This will consist of short answer questions with answer to each question upto 2
pages (250-300 words) in length. Eight questions will be set by the examiner and five have to
be answered by the candidate. Each question will carry 10 marks. Total weightage of the
section shall be 50 marks.
Section-C: This will consist of essay type questions with answer to each question upto 5 pages
(approx 500 words) in length. Four questions will be set by the examiner and two have to be
answered by the candidate. Each question will carry 15 marks. Total weightage of the section
shall be 30 marks.
INSTRUCTIONS FOR THE CANDIDATES: Answer all questions only in required
word.

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & Syllabus :BMLT-1st Year

Paper-I: Basic Histology (Anatomy & Physiology)

ANATOMY PHYSIOLOGY

Syllabus Contents:-

1. The anatomic and physiological organization of human body

and integrated physiology.
2. Cell organization and function.
3. Skeletal system, bones, joints, and muscles.
4. Body fluids and their significance.
5. Blood morphology, chemistry and function.
6. Respiratory system
7. Cardiovascular system viii) Alimentary system, mechanism and
physiology of digestion and absorption
8. Liver structure
9. Urinary system.
10. Male genital system
11. Female genital system.
12. Nervous system.
13. Spleen, lymph node and R.E. system.
14. Endocrine glands and their functions.

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

HISTOTECHNOLOGY
FUNDAMENTALS OF APPLIED HISTOLOGY

INTRODUCTION:

1. Introduction to histopathology and laboratory organization.

2. Laboratory equipment, uses and maintenance.
3. Laboratory hazards and safety precautions.
4. Compound microscope - optical system, magnification and
maintenance.

FUNDAMENTALS OF APPLIED HISTOLOGY

1. Reception, recording and labeling of histology specimens.
2. Fixation and various fixatives.
3. Processing of histological tissues for paraffin bedding.
4. Embedding and embedding media.
5. Decalcification various types, there.
6. Micro tomes various types, there working principle and
maintenance.
7. Microtome knives and knife sharpening.
8. Practical section cutting, cutting faults and remedies.
9. Routine staining procedures, mounting and mounting media.
Dye chemistry, theory and practice of staining.
Solvents, mordents, accelerators and accentuators.
10. Uses of controls in various staining procedures.

CYTOLOGY LECTURES:
1. Introduction to exfoliative cytology with special emphasis on
female genital tract.
2. Collection processing and staining of the Cytologic specimen

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & Syllabus :BMLT-1st Year

Paper-II: Microbiology-I

Theory Internal Practical Total

Subject
Assessment
Microbiology-I 100 100 100 300
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively for
passing the examination and in practical he/she should get 50% marks i.e.-50 marks
to get pass.
There shall be one paper setter external or internal for theory examination and two
examiners, one internal (Chairman) and one external for practical examinations.
Recognized teachers in Microbiology after M.D.-Microbiology/Pathology with five
years of teaching experience shall be on the panel of examiner.
- Theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of
Examination (Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Question Total Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300 words
2 essay type Questions 15 30
Answer to be given in 450-500 words
Total Marks 100
INSTRUCTION FOR THE PAPER SETTER

Section-A: This will consist of 10 very short answer type questions with answer to each
question upto five lines (Fifty to sixty words) in length . All questions will be compulsory to
answer. Each question will carry two marks. Total weightage of the section shall be 20 marks.
Section-B: This will consist of short answer questions with answer to each question upto 2
pages (250-300 words) in length. Eight questions will be set by the examiner and five have to
be answered by the candidate. Each question will carry 10 marks. Total weightage of the
section shall be 50 marks.
Section-C: This will consist of essay type questions with answer to each question upto 5 pages
(approx 500 words) in length. Four questions will be set by the examiner and two have to be
answered by the candidate. Each question will carry 15 marks. Total weightage of the section
shall be 30 marks.
INSTRUCTIONS FOR THE CANDIDATES: Answer all questions only in required
word.

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & Syllabus :BMLT-1st Year

Paper-II: Microbiology-I

Syllabus Contents

MEDICAL MICROBIOLOGY

1. Introduction and brief history of microbiology.

2. Safety measures in microbiology.
3. General characteristics and classification of bacteria and
fungi.
4. Growth and nutrition of microbes.
5. Care and maintenance of laboratory equipments.
6. Care and handling of various microscopes – binocular, DGI,
phase – contrast, fluorescence and electron microscopes.
7. Principles and methods of sterilization.
8. Uses and mode of action of antiseptics and disinfectants.
9. Handling and cleaning of glassware apparatus.
Decontamination and disposal of contaminated material.
10. Preparation, uses and standardization of culture media.
11. Principles of staining methods and preparation of reagents.
12. Aerobic and anaerobic culture methods.
13. General characters and nature of antigens and antibodies.
14. Principles of Antigen Antibody reactions.
15. Collection, transportation and processing of clinical samples
for microbiology investigations.
16. Principles and mode of action of antibiotics and
chemotherapeutic agents for bacteria and fungi.
17. Care and handling of laboratory animals.
18. Laboratory organization, management, recording of results
and quality control in microbiology.
VIROLOGY
1. Introduction to Medical Virology.

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

2. Nomenclature and classification of viruses.

3. General characteristics of viruses: physical, chemical and
biological properties.
4. Collection, transport, processing and storage of sample for
viral diagnosis.
Practical
1. Introduction to use of different laboratory instruments and their
safety precautions.
2. Collection, handling and storage of samples for viral diagnosis.
3. Washing, cleaning and sterilization of media and glassware in
virology.
4. Principles of bio safety hoods, use of pipettes, syringes and
other virus contaminated instruments in the laboratory.
5. Demonstration of preservation of viruses, viral antigens,
infected biological materials and viruses.
PARASITOLOGY
1. Introduction to medical and safety.
2. General characters and classification of protozoa.
3. Laboratory procedure collections, preservation and processing
of samples for parasites stool/blood/fluids/tissue/biopsy.
4. Morphology and life cycles of intestinal protocol, Amoeba-
Giardia.
5. Laboratory diagnosis of intestinal protozoa infection: -
Amoeba-Giardia.
6. Morphology and diagnosis of oral of – trichomonas vaginal
flagellates – E. Gingivalia.
7. Morphology and life cycle of Haemopro- malaria protozoa-
parasite.
8. Laboratory diagnosis of malarial infection.
9. General characters and classification of medical
helminthology.
10. Morphology and life cycles of Nematodes (Intestinal), -
Ascaris, Enterobious, - ancylostoma, - Strongyloides.
11. Laboratory diagnosis of intestinal Nematode infection.

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Practical
1. Introduction to operation of laboratory instruments and safety
precautions.
2. Macroscopic examination of adult worms, cysts, tissues, and
processing of stood sample for routine examination.
3. Saline and I2 preparation for protozoa cysts and trophozoites.
4. Concentration procedures for protozoa cysts and trophoziotes.
5. Concentration procedures for helminthic ova and cyst.
6. Examination and identification of ova and cyst of parasites of
medical importance.

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & Syllabus :BMLT-1st Year

Paper-III: Biochemistry-I

Theory Internal Practical Total

Subject
Assessment
Biochemistry-I 100 100 100 300
Hematology-I 100 100 100 300
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively for
passing the examination and in practical he/she should get 50% marks i.e.-50 marks
to get pass.
There shall be one paper setter external or internal for theory examination and two
examiners, one internal (Chairman) and one external for practical examinations.
Recognized teachers in Biochemistry after M.D./M.Sc. -Biochemistry with five years
of teaching experience shall be on the panel of examiner.
- Theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of
Examination (Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Question Total Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300 words
2 essay type Questions 15 30
Answer to be given in 450-500 words
Total Marks 100
INSTRUCTION FOR THE PAPER SETTER

Section-A: This will consist of 10 very short answer type questions with answer to each
question upto five lines (Fifty to sixty words) in length . All questions will be compulsory to
answer. Each question will carry two marks. Total weightage of the section shall be 20 marks.
Section-B: This will consist of short answer questions with answer to each question upto 2
pages (250-300 words) in length. Eight questions will be set by the examiner and five have to
be answered by the candidate. Each question will carry 10 marks. Total weightage of the
section shall be 50 marks.
Section-C: This will consist of essay type questions with answer to each question upto 5 pages
(approx 500 words) in length. Four questions will be set by the examiner and two have to be
answered by the candidate. Each question will carry 15 marks. Total weightage of the section
shall be 30 marks.
INSTRUCTIONS FOR THE CANDIDATES: Answer all questions only in required
word.

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & Syllabus :BMLT-1st Year

Paper-III: Biochemistry-I

Syllabus Contents
BASIC PRINCIPLES OF BIOCHEMISTRY

1. Introduction to medical technology role of medical laboratory

Technologists, ethics, responsibility, safety, measures First aid
(accidents).
2. Cleaning and care of general laboratory glassware and
equipment, preparation and storage of distilled water analytical
balance, preparation of reagents and standard solutions, storage
of chemicals .
3. Units of measurement, S.I. Units, measurement of volumetric
apparatus, (pipettes, flasks, cylinders) Calibration of
volumetric apparatus.
4. Radioisotopes and their use in Biochemistry, mole, molar and
normal solutions, pH, buffer solutions, pH and pH
measurement, Osmosis, dialysis, surface tension.
5. Urine analysis (qualitative) for sugar, proteins bile pigments,
ketone bodies, porpholinogen, faecal of blood.
6. Collection and recording of biological specimens separation of
serum plasma, preservation and disposal of biological samples
material. Basic statistics (mean, SD, CV, normal distribution,
probability).

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

7. Normal or Reference range. Definition, influencing factors,

determination.
8. Volumetric analysis- Preparation of standard acid and base
solutions, chloride estimation.

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & Syllabus :BMLT-1st Year

Paper-IV: Hematology-I

Theory Internal Practical Total

Subject
Assessment
Hematology-I 100 100 100 300
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively for
passing the examination and in practical he/she should get 50% marks i.e.-50 marks
to get pass.
There shall be one paper setter external or internal for theory examination and two
examiners, one internal (Chairman) and one external for practical examinations.
Recognized teachers in Pathology after M.D. Pathology with five years of teaching
experience shall be on the panel of examiner.
- Theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of
Examination (Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Question Total Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300 words
2 essay type Questions 15 30
Answer to be given in 450-500 words
Total Marks 100
INSTRUCTION FOR THE PAPER SETTER

Section-A: This will consist of 10 very short answer type questions with answer to each
question upto five lines (Fifty to sixty words) in length . All questions will be compulsory to
answer. Each question will carry two marks. Total weightage of the section shall be 20 marks.
Section-B: This will consist of short answer questions with answer to each question upto 2
pages (250-300 words) in length. Eight questions will be set by the examiner and five have to
be answered by the candidate. Each question will carry 10 marks. Total weightage of the
section shall be 50 marks.
Section-C: This will consist of essay type questions with answer to each question upto 5 pages
(approx 500 words) in length. Four questions will be set by the examiner and two have to be
answered by the candidate. Each question will carry 15 marks. Total weightage of the section
shall be 30 marks.
INSTRUCTIONS FOR THE CANDIDATES: Answer all questions only in required
word.

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & Syllabus :BMLT-1st Year

Paper-IV: Hematology-I

Syllabus Contents
HEMATOLOGY

1. Introduction to hematology and Laboratory Organization.

2. Lab.Safety and instrumentation.
3. Formation of blood.
4. Composition and functions of blood.
5. Various anticoagulants, their uses, mode of action and their
merits and demerits.
6. Collection & preservation of blood for various hematological
investigations.
7. Physiological variations in Hb, PCV, TLC and platelet.
8. Normal and absolute values in hematology. 8. Quality assurance
in hematology.
9. Haemoglobinometry, various methods of estimation of Hb,
errors involved and standardization of instrument for
adaptation for Hb estimation.
10. Hemocytometry, procedures for cell counts visual as well as
electronic, red cell, leucocytes and platelet counts. An error
involved and means to minimize such errors.
11. Romanowsky dyes, preparation and staining procedure of the
blood smears.
12. Morphology of normal blood cells and their identification.
13. Erythrocyte sedimentation rate, factors influencing and various
procedures for its estimation with their significance.
14. Haemocrit value by macro and micro methods their merit and
demerits.
15. Routine examination of urine.

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

16. Examination of biological fluids such as CSF, etc.

17. Examination of semen.

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MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SYLLABUS & SCHEME OF

EXAMINATION FOR BMLT
SECOND YEAR

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

BMLT SYLLABUS

SR. NO. SUBJECTS PAGE NO.

ANALYTICAL
1. 03-04
BIOCHEMISTRY

2. HAEMATOLOGY 05-06

3. HISTOLOGY 07

FUNDAMENTELS OF

4. APPLIED HISTOLOGY 08-09

AND CYTOLOGY

5. MICROBIOLOGY 10-11

6. VIROLOGY 13

7. PARASITOLOGY 04 - 15

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

BACHELOR IN MEDICAL LAB TECHNOLOGY (BMLT)

SCHEME Of EXAMINATION:BMLT-2nd Year
Theory Internal Practical Total
S.No. Subject
Assessment
1 Histology 100 100 100 300
2 Microbiology-II 100 100 100 300
3 Biochemistry-II 100 100 100 300
4 Hematology-II 100 100 100 300
Total Max. Marks 1200
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively
for passing the examination and in practical he/she should get 50% marks i.e.-50
marks to get pass.

-All theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of Examination
(Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Total

Question Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300
words
2 essay type Questions 15 30
Answer to be given in 450-500
words
Total Marks 100

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & Syllabus :BMLT-2nd Year

Paper-I: Histology

Theory Internal Practical Total

Subject
Assessment
Histology 100 100 100 300
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively for
passing the examination and in practical he/she should get 50% marks i.e.-50 marks
to get pass.
There shall be one paper setter external or internal for theory examination and two
examiners, one internal (Chairman) and one external for practical examinations.
Recognized teachers in Pathology after M.D. Pathology with five years of teaching
experience shall be on the panel of examiner.
- Theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of
Examination (Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Question Total Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300 words
2 essay type Questions 15 30
Answer to be given in 450-500 words
Total Marks 100
INSTRUCTION FOR THE PAPER SETTER

Section-A: This will consist of 10 very short answer type questions with answer to each
question upto five lines (Fifty to sixty words) in length . All questions will be compulsory to
answer. Each question will carry two marks. Total weightage of the section shall be 20 marks.
Section-B: This will consist of short answer questions with answer to each question upto 2
pages (250-300 words) in length. Eight questions will be set by the examiner and five have to
be answered by the candidate. Each question will carry 10 marks. Total weightage of the
section shall be 50 marks.
Section-C: This will consist of essay type questions with answer to each question upto 5 pages
(approx 500 words) in length. Four questions will be set by the examiner and two have to be
answered by the candidate. Each question will carry 15 marks. Total weightage of the section
shall be 30 marks.
INSTRUCTIONS FOR THE CANDIDATES: Answer all questions only in required
word.

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & Syllabus :BMLT-2nd Year

Paper-I: Histology

Syllabus Contents
HISTOTECHNOLOGY: BASIC CELLULAR PATHOLOGY
AND ALLIED TECHNOLOGY

HUMAN HISTOLOGY

I. Study of various body tissues.

1. Epithelial tissue.
2. Connective tissue including bone and cartilage.
3. Muscular tissue.
4. Nervous tissue.
5. Glands ,epithetical and endocrine glands.
II. Histological study of various system
1. The circulatory system
2. The alimentary system.
3. The digestive system including liver, pancreas and gall
bladder.
4. The respiratory system.
5. The urinary system.
6. The endocrinal gland system
7. The reproductive system
8. Nerve ending and organ of special senses.

FUNDAMENTALS OF APPLIED HISTOLOGY

1. Microscopy ,working principle, maintenance and application of

various types of microscope :-
a) Dark ground microscope
b) Polarizing microscope

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

c) Phase contrast microscope

d) Interference microscope
e) UV microscope
f) Micrometry
2. Metachromasis and metachromatic dyes
3. Haematoxylene its importance in histology.
4. Carbohydrates and amyloid –special stains procedures.
5. Connective tissue, trichome staining and other special stains for
the muscular fibres , elastic.reticulin and collagen fibres.
6. Principle of metal impregnation techniques.
7. Demonstration and identification of mineral pigments.

CYTOLOGY LECTURES:
1. Stain cytologic preparation with special emphasis of
MGG,PAPANICOLOU stains.
2. Special stains like PAS, mucicaramine , alcian blue ,schmorl
and acid phosphates.
3. Cytolologic screening and quality control in cytology
laboratory.

HISTOLOGY& CYTOLOGY PRACTICALS

- Includes practical demonstration of all contents includes in
theory syllabus contents .

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & Syllabus :BMLT-2nd Year

Paper-II: Microbiology-II

Theory Internal Practical Total

Subject
Assessment
Microbiology-II 100 100 100 300
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively for
passing the examination and in practical he/she should get 50% marks i.e.-50 marks
to get pass.
There shall be one paper setter external or internal for theory examination and two
examiners, one internal (Chairman) and one external for practical examinations.
Recognized teachers in Microbiology after M.D. Microbiology with five years of
teaching experience shall be on the panel of examiner.
- Theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of
Examination (Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Question Total Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300 words
2 essay type Questions 15 30
Answer to be given in 450-500 words
Total Marks 100
INSTRUCTION FOR THE PAPER SETTER

Section-A: This will consist of 10 very short answer type questions with answer to each
question upto five lines (Fifty to sixty words) in length . All questions will be compulsory to
answer. Each question will carry two marks. Total weightage of the section shall be 20 marks.
Section-B: This will consist of short answer questions with answer to each question upto 2
pages (250-300 words) in length. Eight questions will be set by the examiner and five have to
be answered by the candidate. Each question will carry 10 marks. Total weightage of the
section shall be 50 marks.
Section-C: This will consist of essay type questions with answer to each question upto 5 pages
(approx 500 words) in length. Four questions will be set by the examiner and two have to be
answered by the candidate. Each question will carry 15 marks. Total weightage of the section
shall be 30 marks.
INSTRUCTIONS FOR THE CANDIDATES: Answer all questions only in required
word.

26
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & Syllabus :BMLT-2nd Year

Paper-II: Microbiology-II

Syllabus Contents:-
MICROBIOLOGY

I. IDENTIFICATION OF BACTERIA
1. Micrococci
2. Staphylococci
3. Streptococci
4. Pneumococci
5. Corynebacteria
6. Escherrichia
7. Kiebsiella
8. Enterobacter
9. Proteus-providencia
10. salmo-nella
11. Shigella
12. Arrizona
13. Citrobacter
14. Yersinia
15. Pseudomonas
16. Vibrio
17. Haemophilus
18. Hydobacteris
19. Brucella
20. Bordetella
21. Bacillus
22. Clostridia
23. Anaerobic cocci
24. Neisseria
25. Treponema

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

26. Borrelia
27. Laptospira
28. Mycoplasma
29. Ricketessia
30. Chlomydia
31. Tric agent

PATHOGENIC AND NONPATHOGENIC FUNGI

1. Candida
2. Cryptococci
3. Dermatophytes
4. Sprotrichoums
5. Histoplasma
6. Blastomyces
7. Coccidioides
8. Para Coccidioides
9. Dematiaceous fungi
10. Mycetoma
11. Actinomyces
12. Nocardia
13. Common laboratory contamins
14. Biochemical test used for the identification of bacteria and
fungi
15. Antimicrobial sensitivity testing
16. Assay methods for body fluids
17. Antimicrobial susceptibility testing for mycobacterium
18. Preparation and standardization of antigen and antisera
VIROLOGY

1. Different staining technique used virology.

2. Used of embryonated eggs in clinical virology
3. Principles of animal cell culture and their use in virology.

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MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

4. Use of common laboratory animals in viral culture.

Practical’s :-
1. Demonstration of staining procedure :-
Preparation of following stains and demonstration of viral
inclusion of bodies:-
a) Seller’s stain for negri body demonstration.
b) Giemsa’s stain for CMV & Herpes viral inclusion.
2. Preparation of reagent for serological tests
Phosphate buffered saline,veronal buffered saline,alsever’s
solution,dextrose gelatin,veronal buffer and tris buffer.
3. Principle and performance of viral haemoagglutination and
haemoagglutination in hibition test.
4. Demonstration of haemadsorption test’IHA & RPHA test
5. Collection,titration and reservation of gunes pig serum for
complement
6. Demonstration of complement fixation test
7. Demonstration of immunofluoroscence test and
immunoperoxidase tests .
8. Demonstration of ELISA for HBsAg detection

PARASITOLOGY

1. Morphology and life cycle of haemoflagellates –

leishmania and trepanosomes.
2. Morphology and life cycle of tissue and blood nematodes
– filariae,trichmella ,dracunculus.
3. Laboratory diagnosis of tissue and blood nematodes
infection :- tanenia,echinococcus.

29
MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

4. Morphology and life cycle of intestinal cestodes – H.nana ,

D.Latum
5. Laboratory diagnosis of cessode infection-
hydatid,cysticerosis.
6. Culture technique for protozoa ,amoeba
,giardia,leishmania.
7. Culture method for helminth’s –hook worm,round worm.
8. Egg counting techniques
9. Putting up cason’s test and its interpretation.
10. Examination and processing of cysticerosis cyst.
11. Laboratory processing,staining and examination of
sample.

30
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS :BMLT-2nd Year

Paper-III: Biochemistry-II

Theory Internal Practical Total

Subject
Assessment
Biochemistry-II 100 100 100 300
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively for
passing the examination and in practical he/she should get 50% marks i.e.-50 marks
to get pass.
There shall be one paper setter external or internal for theory examination and two
examiners, one internal (Chairman) and one external for practical examinations.
Recognized teachers in Biochemistry after M.D. Biochemistry with five years of
teaching experience shall be on the panel of examiner.
- Theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of
Examination (Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Question Total Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300 words
2 essay type Questions 15 30
Answer to be given in 450-500 words
Total Marks 100
INSTRUCTION FOR THE PAPER SETTER

Section-A: This will consist of 10 very short answer type questions with answer to each
question upto five lines (Fifty to sixty words) in length . All questions will be compulsory to
answer. Each question will carry two marks. Total weightage of the section shall be 20 marks.
Section-B: This will consist of short answer questions with answer to each question upto 2
pages (250-300 words) in length. Eight questions will be set by the examiner and five have to
be answered by the candidate. Each question will carry 10 marks. Total weightage of the
section shall be 50 marks.
Section-C: This will consist of essay type questions with answer to each question upto 5 pages
(approx 500 words) in length. Four questions will be set by the examiner and two have to be
answered by the candidate. Each question will carry 15 marks. Total weightage of the section
shall be 30 marks.
INSTRUCTIONS FOR THE CANDIDATES: Answer all questions only in required
word.

31
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS :BMLT-2nd Year

Paper-III: Biochemistry-II

Syllabus Contents:-
BIOCHEMISTRY
ANALYTICAL BIOCHEMISTRY AND METABOLISM

1. Colorimeter

2. Spectrophotometer
3. Flame photometry
4. Atomic absorption spectroscopy
5. Electrometric determination of Na + and K +
6. Chromatography and electrophoresis.
7. Introduction,properties and simple metabolism of
carbohydrates,protein,fats,nucleic and acid and enzymes
8. Digestion and absorption
9. Nutrition (vitamin and calories)
10. Radioimmunoassay (RIA) and ELISA

BIOCHEMISTRY PRACTICAL
1. Study Of Colorimeter
2. Study of spectrophotometer
3. Study of flame photometer
4. Study of gel electrophoresis
5. Study of ELISA

32
MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

6. Study of paper chromatography

7. Study of thin layer chromatography
8. Study of pH meter
9. To prepare phosphate buffer (200 ml p H 7.45 ) and determine
its p H by using meter.
10. Determine the pKa value of acetic acid.
11. Estimation of sugar by DNS method.
12. To extract invertase enzyme from solanum tuberosum (patato).
13. Estimation of protein by lawry’s method.
14. Estimation of protein by DNS method for determining the
invertase activity.
15. Different type of glassware’s and their composition.
16. Preparation of benedict’s qualitative reagent.
17. Estimation of serum glutamate pyruvate transaminase enzyme
(SGPT & ALT).
18. Determination of SGOT
19. Plot a standard graph of SGPT.
20. Plot a standard graph of SGOT.
21. Determination of serum acid phosphatase.
22. To plot a standard graph of serum acid phosphatase.
23. Determination of serum amylase by colorimeteric method.

33
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS :BMLT-2nd Year

Paper-IV: Hematology-II

Theory Internal Practical Total

Subject
Assessment
Hematology-II 100 100 100 300
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively for
passing the examination and in practical he/she should get 50% marks i.e.-50 marks
to get pass.
There shall be one paper setter external or internal for theory examination and two
examiners, one internal (Chairman) and one external for practical examinations.
Recognized teachers in Pathology after M.D. Pathology with five years of teaching
experience shall be on the panel of examiner.
- Theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of
Examination (Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Question Total Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300 words
2 essay type Questions 15 30
Answer to be given in 450-500 words
Total Marks 100
INSTRUCTION FOR THE PAPER SETTER

Section-A: This will consist of 10 very short answer type questions with answer to each
question upto five lines (Fifty to sixty words) in length . All questions will be compulsory to
answer. Each question will carry two marks. Total weightage of the section shall be 20 marks.
Section-B: This will consist of short answer questions with answer to each question upto 2
pages (250-300 words) in length. Eight questions will be set by the examiner and five have to
be answered by the candidate. Each question will carry 10 marks. Total weightage of the
section shall be 50 marks.
Section-C: This will consist of essay type questions with answer to each question upto 5 pages
(approx 500 words) in length. Four questions will be set by the examiner and two have to be
answered by the candidate. Each question will carry 15 marks. Total weightage of the section
shall be 30 marks.
INSTRUCTIONS FOR THE CANDIDATES: Answer all questions only in required
word.

34
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS :BMLT-2nd Year

Paper-IV: Hematology-II

Syllabus Contents
FUNDAMENTALS OF HAEMATOLOGY
1. History and discovery of blood group.
2. ABO and Rhesus blood group system.
3. Compatibility test in blood transfusion, complication and
hazards of blood transfusion.
4. Laboratory investigation of transfusion reaction and mismatched
transfusion.
5. Preparation of packed cells and various fractions of blood for
transfusion purpose.
6. Staining of bone marrow smear and preparation of histological
section
7. Haemoglobin its synthesis function and degradation.
8. Haemoglobin pigments and their measurements.
9. Abnormal haemoglobin and their means of identification and
estimation.
10. LE cells phenomenon and various method of its demonstration.
11. Haemostatic mechanism and theories of blood coagulation.
12. Screening coagulation procedure.
13. Quantitative assay of coagulation factors.

35
MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SYLLABUS & SCHEME OF

EXAMINATION FOR BMLT
THIRD YEAR

36
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

BACHELOR IN MEDICAL LAB TECHNOLOGY (BMLT)

SCHEME Of EXAMINATION:BMLT-3rd Year
Theory Internal Practical Total
S.No. Subject
Assessment
Applied 100 100 100 300
1
Histopathology
2 Microbiology-III 100 100 100 300
3 Biochemistry-III 100 100 100 300
4 Hematology-III 100 100 100 300
Total 1200
5 Instrumentation # -- 50 -- 50
Total Max. Marks 1250
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively
for passing the examination and in practical he/she should get 50% marks i.e.-50
marks to get pass.

-All theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of Examination
(Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Total

Question Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300
words
2 essay type Questions 15 30
Answer to be given in 450-500
words
Total Marks 100

37
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS : BMLT-3rd Year

Paper-I: Applied Histopathology

Theory Internal Practical Total

Subject
Assessment
Applied Histopathology 100 100 100 300
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively for
passing the examination and in practical he/she should get 50% marks i.e.-50 marks
to get pass.
There shall be one paper setter external or internal for theory examination and two
examiners, one internal (Chairman) and one external for practical examinations.
Recognized teachers in Pathology after M.D. Pathology with five years of teaching
experience shall be on the panel of examiner.
- Theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of
Examination (Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Question Total Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300 words
2 essay type Questions 15 30
Answer to be given in 450-500 words
Total Marks 100
INSTRUCTION FOR THE PAPER SETTER

Section-A: This will consist of 10 very short answer type questions with answer to each
question upto five lines (Fifty to sixty words) in length . All questions will be compulsory to
answer. Each question will carry two marks. Total weightage of the section shall be 20 marks.
Section-B: This will consist of short answer questions with answer to each question upto 2
pages (250-300 words) in length. Eight questions will be set by the examiner and five have to
be answered by the candidate. Each question will carry 10 marks. Total weightage of the
section shall be 50 marks.
Section-C: This will consist of essay type questions with answer to each question upto 5 pages
(approx 500 words) in length. Four questions will be set by the examiner and two have to be
answered by the candidate. Each question will carry 15 marks. Total weightage of the section
shall be 30 marks.
INSTRUCTIONS FOR THE CANDIDATES: Answer all questions only in required
word.

38
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS : BMLT-3rd Year

Paper-I: Applied Histopathology

Syllabus Contents:-

SPECIAL HISTOLOGY AND HISTOCHEMICAL METHODS

I. APPLIED HISTOLOGY
1. Handling of fresh histological specimen,cryo/frozen section of fresh and fixed
tissue,freeze drying.
2. Lipid identification and demonstration
3. Micro-organism in the tissue-various staining techniques for their
demonstration and identification.
4. Nucleic acid ,DNA and RNA special stains and procedures.
5. Cytoplasmic constituent and their demonstration.
6. Tissue requiring special treatment i.e eye ball,B.M.biopsy ,under calcified
bones.
7. Neuropathology techniques.
8. Enzyme histochemistry demonstration of
phosphatase,dehydrogenase,oxidase and peroxidase etc.
9. Electron microscope ,their working ,component and allied techniques for
electron microscopy.
10. Ultra microtomy
11. Museum technique.

II. CYTOLOGY
1. Cervical cytology –basis of detection of malignant and pre malignant
lesions.
2. Hormonal assessment with cytological techniques and sex chromatin and
pregnancy test.
3. Aspiration cytology principles, indications and utility of technician in FNAC
clinics.
III. IMMUNOPATHOLOGY
1. cells and organs of immune systems
2. immunoglobulin’s antibodies and humoral immune response.
3. Allergy
4. Rheumatological diseases and investigations.
5. Infection and the immune system.
6. Cancer immunology
7. Tissue typing for kidney transplant.

39
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS : BMLT-3rd Year

Paper-II: Microbiology-III

Theory Internal Practical Total

Subject
Assessment
Microbiology-III 100 100 100 300
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively for
passing the examination and in practical he/she should get 50% marks i.e.-50 marks
to get pass.
There shall be one paper setter external or internal for theory examination and two
examiners, one internal (Chairman) and one external for practical examinations.
Recognized teachers in Microbiology after M.D. /M.Sc. Microbiology with five years
of teaching experience shall be on the panel of examiner.
- Theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of
Examination (Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Question Total Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300 words
2 essay type Questions 15 30
Answer to be given in 450-500 words
Total Marks 100
INSTRUCTION FOR THE PAPER SETTER

Section-A: This will consist of 10 very short answer type questions with answer to each
question upto five lines (Fifty to sixty words) in length . All questions will be compulsory to
answer. Each question will carry two marks. Total weightage of the section shall be 20 marks.
Section-B: This will consist of short answer questions with answer to each question upto 2
pages (250-300 words) in length. Eight questions will be set by the examiner and five have to
be answered by the candidate. Each question will carry 10 marks. Total weightage of the
section shall be 50 marks.
Section-C: This will consist of essay type questions with answer to each question upto 5 pages
(approx 500 words) in length. Four questions will be set by the examiner and two have to be
answered by the candidate. Each question will carry 15 marks. Total weightage of the section
shall be 30 marks.
INSTRUCTIONS FOR THE CANDIDATES: Answer all questions only in required
word.

40
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS : BMLT-3rd Year

Paper-II: Microbiology-III

Syllabus Contents:-

MICROBIOLOGY

I APPLIED MICROBIOLOGY

1.Preservation of microbes and lyophilisation methods.

2.Total and viable count of bacteria.

3.Testing of disinfectant Riedeal –walker,Chick Martin,In use test .

4. Preparation and standardisation of vaccines and immunization schedule.

5.Bacteriological examination of water milk ,food and air.

6.Nosocomial infections and sterility testing of IV fluids and processing of various

samples for hospital infections.

7.Toxin-Antitoxin Assay and pathogenicity tests.

8.Epidemiological markers of micro-organisms serotyping, bacteriophage and

bacteriocin typing method.

9.laboratory diagnosis of common bacterial infections-pyogenic infections,respiratory

tract infections,meningitis, diphtheria ,whooping cough,gas gangrene,food
poisoning,entric fever,acute diarrhoeal disease,cholera,urinary tract
infections,tuberculosis,leprosy,plague,anthrax,typhoid fever,syphilis,gonorrhea and
others STDs disease.

10 . Serological test.

Widal,ASO,LET,CRP, Rosewaller brucella agglutination,cold

agglutination,VDRL,TPHA FTA – ABS.

11. laboratory diagnosis of fungal infections

Superficial dermatophyte, fungal infections, candidiasis infection,

cryptococcosis,pulmonary infections,Mycetoma, other deep mycotic
infections,subcutaneous fungal infections - Sporotrichosis, chromoblasato mycosis,
eye and ear fungal infections.

12 .Serological test for fungal infection and skin tests.

13.Advance techniques in microbiology – ELISA , RIA ,CCIE, Coagglutination

41
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

,GLC,HPLC etc.

14.Rapid diagnostic method and automation in microbiology.

II. BASIC VIROLOGY METHODS

1.Principles of serology techniques used in virology – PART I : HA,HAI,HAB,SRB

RPHA,JHA,CET,CIEP.

2. Principles of serology techniques used in virology – PART II Ht ,

ELISA,RIA,IF,Immuno-ferooxidase test.

3. Mode of transmission of viral agent.

4.Prevention of viral diseases.

5. Immunity of viral infections.

Practical’s

1. Demonstration of anatomical structure in fertile hen’s egg –technique of

inoculation of fertile egg-
a. Chorioallantoic
b. Membrane
c. Allantoic cavity
d. Yolk sac
2. Inoculation of virus infected material into the mice by the following route :-
a. Intracerebral
b. Intravenous
c. Intraperitoneal
d. Subcutaneous
3. Harvesting of infected of infected mouse brain for rabies virus.
4. Preparation of tissue culture media :-
a. Hank’s balance salt solution
b. Barle’s balanced salt solution
c. Minimum essential medium
5. Collection of blood from :-
a. Mice - retro orbital route
b. One day old chick –cardiac bleeding
6. Preparation of guinea pig kidney powder for Paul Bunnel Test.
7. Demonstration of Arboviral Antigen preparation from mouse brain for HAI and
CFT Test.
8. Demonstration of herpes viral antigen in tissue culture system .

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

III PARASITOLOGY

i. Morphology and life cycle of :- Free Living Amoeba Balantidium

Toxoplasma

ii. Diagnosis of morphology and life cycle of tramatiodes :- schistosomos

Intestinal flukes
Blood flukes
Lung flukes
iii. Serological & immunological technique used for the diagnosis of :- gel-
diffusion
THFA, IFA. ELISA, indirect fluorescent antibody
Iv. Introduction to the biological identification of adult :- mosquitoes
Flies
Tics and fleas
Animal care and handling and its uses in parasitological preparation of parasitic
antigen and
antisera.handling and operation of sophisticated equipments.

Practical’s

1. Serological and immunological test use in parasitological

a. Gel diffusion technique
b. Electrophoretic technique
c. Preparation of various parasitic antigen and standardization.
d. Differentiation of various mosquitoes flies,worms and ticks.
e. Uses of laboratory animals bleeding and inoculation.

43
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS : BMLT-3rd Year

Paper-III: Biochemistry-III

Theory Internal Practical Total

Subject
Assessment
Biochemistry-III 100 100 100 300
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively for
passing the examination and in practical he/she should get 50% marks i.e.-50 marks
to get pass.
There shall be one paper setter external or internal for theory examination and two
examiners, one internal (Chairman) and one external for practical examinations.
Recognized teachers in Biochemistry after M.D. Biochemistry with five years of
teaching experience shall be on the panel of examiner.
- Theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of
Examination (Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Question Total Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300 words
2 essay type Questions 15 30
Answer to be given in 450-500 words
Total Marks 100
INSTRUCTION FOR THE PAPER SETTER

Section-A: This will consist of 10 very short answer type questions with answer to each
question upto five lines (Fifty to sixty words) in length . All questions will be compulsory to
answer. Each question will carry two marks. Total weightage of the section shall be 20 marks.
Section-B: This will consist of short answer questions with answer to each question upto 2
pages (250-300 words) in length. Eight questions will be set by the examiner and five have to
be answered by the candidate. Each question will carry 10 marks. Total weightage of the
section shall be 50 marks.
Section-C: This will consist of essay type questions with answer to each question upto 5 pages
(approx 500 words) in length. Four questions will be set by the examiner and two have to be
answered by the candidate. Each question will carry 15 marks. Total weightage of the section
shall be 30 marks.
INSTRUCTIONS FOR THE CANDIDATES: Answer all questions only in required
word.

44
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS : BMLT-3rd Year

Paper-III: Biochemistry-III

Syllabus contents:-

CLINICAL BIOCHEMISTRY METHODS

1. Principle for assay procedure for biological material-

i. Total protein
ii. Total albumin
iii. Glucose
iv. Urea
v. Uric acid
vi. Creatinine
vii. Cholesterol
viii. Bilirubin
ix. Sodium
x. Potassium
xi. Chloride
xii. Calcium
xiii. Inorganic phosphates
xiv. PBD 17 ketosterious
xv. Barbiturates
2. Glucose tolerance test
3. Insulin tolerance test gastric analysis
4. Xylose absorption test
5. Clearance test for renal function
6. Enzyme-acid and alkaline phosphatase
7. AST
8. ALT
9. Amylase lactate dehydrogenase
10. CPK
11. Analysis of calculi and CSF
12. Quality control of clinical investigations
13. Automation in clinical biochemistry laboratory
14. Laboratory organizations
15. Management and maintenance of records

45
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS : BMLT-3rd Year

Paper-IV: Hematology-III

Theory Internal Practical Total

Subject
Assessment
Hematology-III 100 100 100 300
N.B.-Internal Assessment marks will be added in theory marks; candidate have to
get min. 50% marks i.e.-100 marks in theory and internal assessment collectively for
passing the examination and in practical he/she should get 50% marks i.e.-50 marks
to get pass.
There shall be one paper setter external or internal for theory examination and two
examiners, one internal (Chairman) and one external for practical examinations.
Recognized teachers in Pathology after M.D. Pathology with five years of teaching
experience shall be on the panel of examiner.
- Theory papers will of 100 max. marks and 3Hrs. time duration . Pattern of
Examination (Theory) if Maximum Marks are 100 will be as under;-

No. and Type of Questions Marks for each Question Total Marks
10 very short answer Questions 02 20
Answer to be given in 50-60 words
5 short answer Questions 10 50
Answer to be given in 250-300 words
2 essay type Questions 15 30
Answer to be given in 450-500 words
Total Marks 100
INSTRUCTION FOR THE PAPER SETTER

Section-A: This will consist of 10 very short answer type questions with answer to each
question upto five lines (Fifty to sixty words) in length . All questions will be compulsory to
answer. Each question will carry two marks. Total weightage of the section shall be 20 marks.
Section-B: This will consist of short answer questions with answer to each question upto 2
pages (250-300 words) in length. Eight questions will be set by the examiner and five have to
be answered by the candidate. Each question will carry 10 marks. Total weightage of the
section shall be 50 marks.
Section-C: This will consist of essay type questions with answer to each question upto 5 pages
(approx 500 words) in length. Four questions will be set by the examiner and two have to be
answered by the candidate. Each question will carry 15 marks. Total weightage of the section
shall be 30 marks.
INSTRUCTIONS FOR THE CANDIDATES: Answer all questions only in required
word.

46
 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS : BMLT-3rd Year

Paper-IV: Hematology-III

Syllabus Contents

HAEMATOLOGY

APPLIED HAEMATOLOGY

1. Definition and classification of Anaemia’s.

2. Laboratory investigations of megaloblastic anaemia.
3. Laboratory investigations of iron deficiency anaemia .
4. Laboratory investigations of haemolytic anaemia including classification and
causes.
5. Leukaemia :-definition and classification
6. Cytochemical staining procedures in various haemopioetic disorder.
7. Laboratory test for assessing bleeding disorder.
8. Laboratory investigation for disseminated intravascular coagulation
9. Mechanism of fibrinolysis : Test for fibrinolysis
10. Platelets function test and their interpretation.
11. Techniques available for cytogenetic studies.
12. Uses of radio-isotopes in haematology.
13. Safety measures for handling radio-isotopes.

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 MADHYAPRADESH MEDICAL SCIENCE UNIVERSITY, JABALPUR (M.P.)

SCHEME Of EXAMINATION & SYLLABUS :BMLT-3rd Year

Theory Internal Practical Total
Subject
Assessment
Instrumentation # -- 50 -- 50
N.B.-There will be institutional examination/practical demonstrations of following
instruments and procedures of 50 max. marks, where min.25 marks are required
to get pass and after duly compilation of examination the marks have to sent to
university prior to theory examination .
INSTRUMENTATION

1. SIMPLE MICROSCOPY
2. Compound microscopy
3. Dark ground microscopy
4. Phase contrast microscopy
5. Fluorescence microscopy
6. PTL metry
7. Photometry electro
8. Spectrophotometry
9. Haemoglobinometry
10. Haemocytometer
11. hematocrit
12. centrifuge
13. Sterilization instrument(Autoclave, Hot air oven, Laminar air flow )
14. Egg incubator and dental drill
15. Co incubator
16. Bacteriological incubator
17. Microtome and accessories
18. Tissue floatation bath
19. Tissue meton.
20. Serological water bath
21. Micropipettes and microlitre plates
22. Cellephan tubes and bags.
23. Paper and gel electrophoresis
24. Flame photometer
25. Polarizing microscope
26. Micro-hematocrit centrifuge
27. Gas chromatography
28. Radio immune assay
29. Auto analyzers
30. ECG

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