Pouch Products

Dip Slides

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 BACTASLYDE : Comparison with Other Methods
No. of Steps Trained Pre treatment Capital
Methods Maintenance Convenience
involved manpower of sample Investment

Plate Count Method 6 Required Not Required Required Tedious Required

Most Probable Number 6 Required Not Required Required Tedious Required

Membrane Filter 6 Required Not Required Required Tedious Required

Direct Counts 4 Required Required Required Convenient Required

Bioluminescence 3 Required Essential Required Convenient Required

Direct Epifluorescence 6 Required Essential Required Convenient Required

Technique (DEFT)

BACTASLYDE 1 Not Required Not Required Not Required Most Not Required
Convenient

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 SULPHATE REDUCING BACTERIA (SRB) TEST KIT
BS 115

Sulfate Reducing Bacteria (SRB) obtain energy by oxidizing organic compounds or molecular hydrogen while reducing sulfate to hydrogen sulfide. They
are also known as Sulfide Generating Bacteria (SGB) and commonly found in anaerobic environments where they aid in the degradation of organic
materials like secondary oil recovery effluents, estuarine waters, deep wells, cooling waters, metalworking fluids, water-based hydraulic fluids, and
petroleum based products. They can survive up to 60°C and cause pitting corrosion on metal structures that are exposed to sulfate-containing water by
cathodic depolarization, since sulfate is also found in some formulations in water treatment, viz, corrosion inhibitors, coolants, etc. Sulfate acts as a
hydrogen acceptor produces hydrogen sulfide and ferrous sulfide. These together form a malodorous black slime. In some case bubbles of H2S rise though
the water and cause objectionable odors. The corrosion of iron by sulfate reducing bacteria is rapid and unlike ordinary rusting, is not self – limiting. Our
specially developed test kit with a unique 40ml sample size gives a much reproducible quantitative analysis of the SRB count in a sample.

Packaging
Outer The media used in BACTASLYDE B S-1 15 has been specially formulated
Sterile bag
for the growth of the sulphate utilising species, viz. Desulfovibrio,
Desulfotomaculum and Desulfobacter. It contains neutralising compounds,
which would nullify the effect of any inhibitor in the test sample. Hence
Tube instances of false positive (or false negative) results are greatly reduced.
Conventional methods are generally riddled with many such uncertainties.
SRB BACTASLYDE B S-1 15 is designed to circumvent MOST uncertainties.
Black Cap
Medium
Pouch Page 9
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 How to use NOTE
1. Shake the SRB media pouch of BACTASLYDE BS -115 so as to mix the contents of media properly. W hen the blackening takes place within 12 hrs. or
2. Cut open the SRB media pouch and pour the contents of the pouch into the 'tube', completely. overnight the SRB levels could be higher than 105 per
100 ml. Wait for complete blackening of the contents of
3. Fill the 'tube' right upto the brim (top) with the test liquid, to exclude any air. Close the 'tube' the 'tube'. However even a slight blackening on the 6 th
firmly with its cap.
day should be considered as positive, denoting SRB levels
4. Invert the 'tube' to gently mix the media and the test liquid. Do not shake vigorously. to be less than 10 per 100 ml.
5. Keep the 'tube' in a standing position for incubation, at room temperature. Absolutely no blackening even on the 6th Day, denotes
6. Do not shake/invert the 'tube' during the incubation period. SRB levels to be less than 2 per 100 ml. Blackening which
7. Observe for complete blackening of the contents in the 'tube' every 24 hours upto 6 days. takes place within two hours is not to be considered
positive, since this probably will be due to the presence of
*Sulphate reducing bacteria produce hydrogen sulphide as they grow, which reacts to form iron
sulphide resulting in blackening of the 'tube' contents. The blackening of the media would begin sulphide ion in the sample.
at the bottom of the 'tube'. Once the process of sulphide generation begins the blackening
increases fairly rapidly. Storage
BACTASLYDE B S-1 15 is best preserved in a refrigerator
Interpretation (15 °C- 20 °C). If a refrigerator is not available, store at
room temperature in a cool place protected from heat,
Blackening SRB light and draught. If stored this way the Product will keep
Hours Days per 100 ml
for 12 months from the date of manufacture. BACTASLYDE
Tube 24 1 10 5 should not be frozen.
48 2 10 4
Disposal
72 3 10 3
After use pour the contents of the tube into the toilet and
96 4 10 2 flush immediately. Add any disinfecting solution, eg. Dettol,
120 5 10 1 Savlon, Phenol etc. in the empty tube and discard the
1) Pour Media & 2) Incubate & 3) Blackening Confirms 144 6
Test sample Observe Presence of 'SRB' <10 content in the same manner as above, after a couple of hours.
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 BACTASLYDE : Application in Different Industries
Industry Application Bactaslyde Code
Paint & Pigments Process & production waters, raw materials, BS 101, BS 102, BS 103
Finished products (water based paint & pigments)

Metal Working Fluids Cutting oils, coolants in the sump, raw water (make-up) BS 101, BS 103, BS 115

Cooling Water Systems Make-up waters, cooling waters, (recirculating BS 103, BS 115, BS PP1
water, basin water) Close systems BS 125, BS 130

Food Processing Raw materials (e.g. cream, milk, meat, fish, vegetables), BS 101, BS 102, BS 103,
Industry Water used for production and cleaning purpose, BS PP2, BS PP3, BS PP4
finished products (e.g. meat / fish preparations, cakes,
soups, sauces, jams, squashes,spices)

Pulp & Paper Industry White waters (slurry and pulp mixtures), BS 101, BS 103, BS 115
process waters BS 125

Sugar Industry Primary, mixed, clarified juice, process waters, BS 101, BS 103, BS 115
finished sugar

Water Treatment Laboratory and onsite, evaluation of biocides BS 103, BS 115, BS PP 1

Chemical Manufacturers BS 125, BS 130

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 Industry Application Bactaslyde Code
Manufacturers Process waters, production water,
Bulk Drug (demineralized water) BS 103, BS 115

Manufacturers Monitoring of waste water at different stages BS 102, BS 103, BS 115

Water & Waste Water BS 125
Treatment Systems

Cosmetics Process waters. (demineralized water) BS 101, BS 102, BS 103,

raw materials, finished product. BS PP 2, BS PP 3

Dairy Raw milk, pasteurised milk, evaluation BS 101, BS 102, BS 103 ,

of the cleaning-in-process. BS PP 2, BS PP 3

Brewery Process waters, pasteurised Beer BS 101 , BS 102,

fermentation broth. BS 103, BS 115
Water Based Process waters, raw materials, finished product BS 101, BS 103, BS 115
Adhesives

Oil and Petroleum Injection waters, fuel (petrol, aviation) BS 101, BS 103, BS 115

Fisheries Ponds, Sea water, Processed products BS 102, BS PP 2, BS PP 3, BS PP4

BS 101 - Yeasts & Fungi + TBC BS 102 - Escherichia coli + TBC BS 103 - Pseudomonas + TBC BS 115 - SRB BS 125 - Algae Species
BS PP1 - Iron Bacteria BS PP 2 - Salmonella Species BS PP 3 - Staphylococcus Species BS PP 4 - Vibrio Species. BS130 : Nitrifying / Denitrifying Bacteria
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 Our Other Product Range

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