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Botanical

1) The document describes a laboratory exercise that assesses the molluscicidal activity of asyang (Mikania cordata) leaves and vines against the golden snail (Pomacea canaliculata), a serious pest of rice. 2) In the experiment, asyang is soaked in water for 24 and 48 hours to make leachates, which are then applied to rice seedlings and golden snails. Mortality of the snails and damage to seedlings is observed and calculated over time. 3) The objectives are to assess the molluscicidal activity of asyang against golden snail and determine any effect of the leachates on rice seedlings. This helps evaluate as

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Anni Quimverly Crencia
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113 views

Botanical

1) The document describes a laboratory exercise that assesses the molluscicidal activity of asyang (Mikania cordata) leaves and vines against the golden snail (Pomacea canaliculata), a serious pest of rice. 2) In the experiment, asyang is soaked in water for 24 and 48 hours to make leachates, which are then applied to rice seedlings and golden snails. Mortality of the snails and damage to seedlings is observed and calculated over time. 3) The objectives are to assess the molluscicidal activity of asyang against golden snail and determine any effect of the leachates on rice seedlings. This helps evaluate as

Uploaded by

Anni Quimverly Crencia
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Name : Anni Quimverly Crencia Date: September 1,

2022
Course, Year & Sec. BSA 2E Score: ______

Laboratory Exercise No. 2


BOTANICAL CONTROL OF GOLDEN SNAIL (POMACEA CANALICULATA LAMARCK)
USING ASYANG (Mikania cordata)

Introduction

Golden snail or golden kuhol is one of the serious pests of lowland rice. Although it has
been introduced in the Philippines in the early 1980’s as protein rich food, the snail is considered
by the farmers as an enemy of the rice plant. It has a very high reproductive capacity. One snail
can produce an average of 300 individual eggs per egg mass. The newly laid eggs at pinkish and
are found about one foot above the ground. During heavy infestation, missing hills of rice can be
observed in the field. With these missing hills a reduction in grain yield is expected.
One way to control golden kuhol is by using chemical specifically the synthetically
produced molluscicides. However, reports indicated that these can cause toxicity to the
applicators and might cause environmental pollution. Research on the molluscicidal property of
plants for possible control on golden snail have been conducted. These plants which are
biologically active contain substances that are being leached to the medium causing injury to the
snail and death in severe cases. A number of plant species have been studied for molluscicidal
activity. Some of these are Mikania cordata, Tinosphora rhumphii, Derris sp., Ageratum
conyzoides, Chromolaena odorata and others.

Objectives

At the end of the exercise, the students should be able to:

1. Assess the molluscicidal activity of asyang against golden snail and


2. Determine any effect of the leachates on rice seedlings.

Materials

Leaves and vines of asyang and golden snail, plastic pail with cover, nylon tulle, plastic
pots, rice seedlings, knives/scissors, weighing scale, record book and screen to cover the pail.

Methodology

1. Sow rice seeds on sterile soil and after one week, transplant ten (10) seedlings to
plastic pots containing flooded soil (about 10 cm deep). Provide 3 plastic pots of the
following treatments:
1) Asyang leachate soaked for 24 hours
2) Asyang leachate soaked for 48 hours
3) Water (Control)
2. Collect 1000g healthy asyang leaves and vines excluding the roots and bring to the
laboratory.
3. Cut the asyang into small pieces (2-5 cm long) and wash using tap water. Drain for
few minutes.
4. Soak the previously cleaned asyang in 2000 ml water for 48 hours in black plastic
pail with cover.
5. Collect the leachate (liquid portion) after the designated time. Pour 300 ml of the
leachates in one of the plastic pots with rice seedlings.
6. Count the initial plant height and number of open leaves of rice seedlings.
7. Place five (5) previously collected golden snail (more or less of the same size and
age and diameter of the operculum) into each pot.
8. Observe the behavior of the snail upon introduction (until about 1 to 3 hours after
treatment and record your observations.
9. Count the number of dead snails from each pot after 24 and 48 hours. Dead snails
will be observed floating on the surface of the water.
10. Compute the percent snail mortality for all treatments and tabulate the data. Use the
formula:

% Snail Mortality ₌ Number of Dead Snails × 100


Total number of Snails

11. Determine the percent damage by counting the number of seedlings damaged by the
golden snail and compute using the formula:

% Snail Damage ₌ Number of Damaged Seedlings × 100


Total number of Seedlings

12. After three (3) weeks, measure the plant height of the rice seedlings and count the
number of open leaves.

13. Discuss the results and provide supporting literatures to support your findings.

Questions

1. Enumerate other strategies to control golden snail infestation?


2. Explain the advantages of using botanicals for golden snail control as compared with
other methods.

Reference

Borines, L.M., MJ.C. Ceniza, L.H. De Pedro, R.M. Gapasin, R.G. Gerona, J.L. Lim, Y.C.
Mangaoang, et al. 2011. Laboratory manual in plant protection 22: principles of
plant protection. Department of Pest Management, College of Agriculture and
Food Science, Visayas State University. Visca, Baybay City, Leyte.
Suggested Readings

Bidin, Z. 2002. Use of rotten jackfruit to control golden apple snail. Retrieved September 17,
2019. http://www.fftc.agnet.org/library.php?func =view&style=type
&id=20110902151450

PhilRice. Management options for the Golden Apple Snail.


https://www.applesnail.net/pestalert/management_guide/pest_management.ph
p

http://www.knowledgebank.irri.org/step-by-step-production/growth/pests-and-diseases/
golden-apple-snails

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