Scribd
Upload
48 views

Quiz On PCR

The document is a 10 question quiz about the polymerase chain reaction (PCR) process. It asks about the three steps of PCR - denaturing, annealing, and extension. Denaturing heats DNA to separate strands, annealing lowers the temperature for primers to attach, and extension uses DNA polymerase to build new strands from 5' to 3' on each original strand, resulting in 2 new DNA strands formed after each cycle.

Uploaded by

Trinity Marie
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
48 views

Quiz On PCR

The document is a 10 question quiz about the polymerase chain reaction (PCR) process. It asks about the three steps of PCR - denaturing, annealing, and extension. Denaturing heats DNA to separate strands, annealing lowers the temperature for primers to attach, and extension uses DNA polymerase to build new strands from 5' to 3' on each original strand, resulting in 2 new DNA strands formed after each cycle.

Uploaded by

Trinity Marie
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 1

Trinity Marie Hablan Grade 8 – Aster

Biotech Quiz

1. What does PCR stands for? Polymerase Chain Reaction


2. How many steps does the process involve? Three steps
3. What are these steps called (in order)? Denaturing, Annealing, Extension
4. Define denaturation? Process modifying the molecular structure of a protein
5. Up to how many degrees (Celsius) is DNA heated? 60 to 95 Degrees Celsius
6. How many minutes is it heated? 5 minutes
7. What 2 agents are added to the separated DNA to start polymerisation?
8. How many degrees is DNA lowered to start annealing? 45 to 60 Degrees Celsius
9. What is the direction of extension of DNA in order to form two new DNA strand? Left to right
on one strand, and right to left on the other
10. How many new DNA strands will be form after 1 cycle of PCR? 2 DNA strands

You might also like