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DISCUSSION

Microorganisms are living things too small to be seen with the naked eye, including bacteria, archaea, fungi, algae and protozoa. In an exercise, students viewed various microorganisms under a microscope, including algae with chloroplasts, fungi such as yeast and bread molds, and the ciliated protozoan. Escherichia coli (E. coli) bacteria was also viewed at 1000x magnification and researched online. The total magnification was calculated using the eyepiece magnification multiplied by the objective lens magnification.

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Angel Rose Salinasal
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63 views

DISCUSSION

Microorganisms are living things too small to be seen with the naked eye, including bacteria, archaea, fungi, algae and protozoa. In an exercise, students viewed various microorganisms under a microscope, including algae with chloroplasts, fungi such as yeast and bread molds, and the ciliated protozoan. Escherichia coli (E. coli) bacteria was also viewed at 1000x magnification and researched online. The total magnification was calculated using the eyepiece magnification multiplied by the objective lens magnification.

Uploaded by

Angel Rose Salinasal
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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Microorganism is a living thing that is too small to be seen with the naked eye.

It can be a bacteria,
archaea, fungi, algae and a protozoa, in which it can occur in different forms and sizes.

In the exercise, the students viewed some microorganisms including algae, protozoa and fungi. Since the
bacteria wasn't viewed in person, it is then required to searched it on the internet providing its
reference, type of bacteria and the total magnification of the microscope. The chosen type of bacteria
was Escherichia coli (E. coli), a bacterium commonly found in the gut of warm-blooded organisms. Most
strains of E. coli are not harmful but are part of the healthful bacterial flora in the human gut. However,
some types can cause illness in humans, including diarrhea, abdominal pain, fever, and sometimes
vomiting (MediLexicon International). The E. coli was viewed under the microscope with a total
magnification of 1000x in High Power Objective (HPO). The provided reference can be seen on Table 3.
The Bacteria under HPO.

Next is Algae, it is a plant-like organism that commonly found in a range of aquatic habitats. It is viewed
under the microscope using the Scanning Objective with a total magnification of 40x, Low Power
Objective (LPO) with a total magnification of 100x and it is also viewed in HPO with 400x total
magnification. The algae viewed under the microscope was a single strand having a green pigment
because of the chloroplast and it has divisions.

Protozoa are one-celled animals commonly found in stagnant water. It can be ciliates, flagellates,
amoebas or sporozoans. The samples was obtained from the pond water that can be found around the
campus and then viewed under the microscope. Since the protozoa are moving faster, it is barely seen
under the microscope. It was seen in the LPO with a total magnification of 100x and has never been seen
again. The protozoa that was seen was a ciliated protozoa that has a hair-like organelles called cilia.
Since the protozoa cannot be seen anymore, it was then searched on the internet with the same
structure that has been seen in the microscope in person, a protozoa having a cilia.

There are two kinds of fungi that was viewed under the microscope using the Scanning Objective, LPO
and HPO. One of it was the commercial Yeast. It was observed using the Scanning Objective with a total
magnification of 40x, under LPO with a total magnification of 100x and using the HPO having a total
magnification of 400x. The yeast that was viewed has different shapes but mostly circles and it has small
circles and some air bubbles (refer to Table 4. The Fungi - Yeast). The other type of fungi was the bread
Molds. It was also viewd under the microscope using the Scanning Objective, LPO and HPO with the
same total magnification from the Yeast, Scanning Objective - 40x, LPO - 100x and HPO - 400x. The
observed molds consists of filaments (hyphae) that branch to form a feeding structure, a mycelium
(Briggs, G. M.) when viewed under Scanning Objective.

The total magnification of each objective lens that has been used in viewing the samples were calculated
using the formula, Total Magnification = Magnification Power of the Eyepiece x Magnification Power of
the Objective Lens. The magnification power of the eyepiece of the microscope that was used in the
experiment has 10x magnification power and each objective lens from Scanning Objective has a
magnification power of 4x, the LPO has 10x magnification power and the HPO has 40x magnification
power. And it is then calculated using the formula.

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