GE Healthcare

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For electrophoresis results you can count on
84391 GE Folder.qxd 10/11/05 6:52 pm Page 2

Meeting your
electrophoresis needs
today and tomorrow
Since the first Multiphor™ electrophoresis
system was introduced by LKB 30 years
ago, we have continued to provide
innovative electrophoresis solutions to
the life sciences research community,
under the brand names of LKB™, Hoefer™,
Pharmacia™ Biotech, and most recently
Amersham Biosciences.

Today and in the future you can turn to

GE Healthcare for all your electrophoresis
needs. You can rely on the consistent
performance and quality support that has
earned our electrophoresis systems and
products tens of thousands of citations in
scientific publications worldwide.

madetoworktogether
The reliability of your research results
ultimately depends on the quality of the
research tools you use. GE Healthcare
offers an extensive range of kits and
reagents that are made to work together
with your application. Trusted by
thousands of the most demanding
scientists worldwide, our products are
backed by outstanding technical support,
assuring you of results you can trust
every time.

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Electrophoresis application areas
GE Healthcare offers a wide range of instrumentation,
precast gels, reagents, and support materials designed for
the qualitative and quantitative analysis of any biomolecule
studied by electrophoresis.

Page
Discovery 4
Separation of unknown protein samples for
downstream analysis and/or identification

Molecular characterization 5
Determination of molecular weight (Mr),
isoelectric point (pI), subunits, and stability

Purity confirmation 6
Determination of product purity in a
non-regulated environment

Quantitative determination 7
Analytical assessment of abundance

Quality assurance and quality control 8

Samples analyzed for the presence of contaminants,
or to confirm uniformity in your end products

Clinical diagnostic research 9

Screening of patient samples

System selection guide 10

Choose your route to electrophoresis results you
can count on
Further information can be found at
www.madetoworktogether.com.

3
Discovery
Separation of unknown protein samples for
downstream analysis and/or identification

Protein first The major requirements for discovery are Protein 1-D electrophoresis
dimension of 2-D resolution and separation of your sample
electrophoresis in a manner that is compatible with any High resolution, low to SE 600 Ruby, MultiTemp™ III
medium throughput Thermostatic Circulator, & EPS 601
subsequent downstream analysis Power Supply
techniques. Typically, this is either 1-D
or 2-D electrophoresis on a large-format
gel. 2-D electrophoresis involves Protein 2-D electrophoresis
separating the first dimension on an
Immobiline DryStrip gels, isoelectric focusing (IEF) gel, followed Low throughput SE 600 Ruby, MultiTemp III Thermostatic
comprehensive selection Circulator, Multiphor II Electrophoresis
of pH ranges and lengths
by SDS-PAGE in the second dimension.
System, & EPS 3501 XL Power Supply
Medium throughput Ettan DALTsix Large Vertical System
Prior to analysis, efficiently prepare your
with DALT gels, Ettan IPGphor 3 IEF
samples using our integrated range of System with Immobiline DryStrip gels,
Ettan™ Sample Preparation Kits and MultiTemp III Thermostatic Circulator, &
PlusOne™ reagents to ensure consistent EPS 601 Power Supply

and reproducible 2-D results. High throughput Ettan DALTtwelve with DALT gels &
Ettan IPGphor 3 IEF System with
Immobiline DryStrip gels
GE Healthcare is the premier
Ettan IPGphor 3 electrophoresis supplier specializing in
IEF System large-format gel systems. SE 600 Ruby™
is ideal for 1-D electrophoresis analysis
Protein second applications requiring the best in
dimension of 2-D separation. Ettan DALT systems use DALT
electrophoresis precast large-format gels (26 x 20 cm)
for separation in the second dimension
of 2-D electrophoresis. Focusing in the
first dimension of 2-D electrophoresis
can be carried out using Immobiline™
DryStrip gels on the Ettan IPGphor™ 3
or Multiphor II Electrophoresis Systems.

Ettan DALTtwelve
Large Vertical System For sensitive post-staining of gels, use
Deep Purple™ Total Protein Stain to
deliver excellent protein quantification.

Ettan DALTsix SE 600 Ruby

Large Vertical System

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Molecular
characterization
Determination of molecular weight (Mr),
isoelectric point (pI), subunits, and stability

Protein The most traditional application for

electrophoresis is to determine the
molecular weight (Mr) of a protein or
DNA fragment. GE Healthcare offers a
range of electrophoresis units and gels
Multiphor II Electrophoresis
perfect for Mr determination. The larger
System, MultiTemp III the format, the more accurate the Mr
Thermostatic Circulator
determination.
and Processor Plus

For proteins, this is usually done on an

SDS-PAGE system such as Multiphor II
Electrophoresis System with ExcelGel™
precast gels. Whereas for DNA, agarose
gels are used, with either a submarine
gel unit or a pulsed-field electrophoresis
ExcelGel for SDS-PAGE
with Multiphor II
gel unit. Isoelectric points (pl) are
Electrophoresis System determined by IEF (e.g. using
Ampholine™ PAG plate gels), while
Nucleic acid subunits and stability can be
determined using a variety of PAGE
and agarose systems.
Protein
HE 33 Mini Submarine Unit and HE 99X
Max Submarine Unit are ideal agarose High resolution/Mr, SE 600 Ruby, EPS 601 Power Supply
HE 99X Max Submarine Unit
submarine units for DNA applications. subunits, and stability & MultiTemp III Thermostatic Circulator
Gene Navigator™ Pulsed Field System Versatile, high resolution, Multiphor II Electrophoresis System,
separates DNA from 20 kb to 10 Mb. subunits, Mr, pl, and stability MultiTemp III Thermostatic Circulator,
& EPS 3501 XL Power Supply with
ExcelGel and Ampholine Precast gels

Gene Navigator Highest resolution/Mr, SE 660 Ruby, EPS 601 Power Supply, &
Pulsed Field System subunits, and stability MultiTemp III Thermostatic Circulator

Nucleic acid

Small format size determination HE 33 Mini Submarine Unit & EPS 301
and blotting Power Supply/VacuGene™XL
Large format size determination HE 99X Max Submarine Unit & EPS 301
and blotting Power Supply/VacuGene XL
For large fragment analysis Gene Navigator Pulsed Field System,
EPS 601 Power Supply, and MultiTemp III
Thermostatic Circulator
HE 33 Mini Submarine Unit

5
Purity
confirmation
Determination of product purity
in a non-regulated environment

Protein When using techniques such as Protein

chromatography or PCR in protein and
nucleic acid analysis, you often need Small format SE 250 or SE 260 Mini-Vertical Units or
a rapid method to confirm that you miniVE Vertical Electrophoresis System
& EPS 301 Power Supply
have isolated a pure sample, with no
High throughput, PhastSystem, PhastGel, PhastGel Protein
contamination. Electrophoresis is an semi-automated Silver Staining Kit, and PlusOne
PhastSystem excellent way to check for purity. This Coomassie Tablets, PhastGel Blue R-350
type of application requires techniques
that are fast, reliable and easy to run.
Nucleic acid Nucleic acid
For protein analysis, miniVE Vertical
Electrophoresis System, SE 250 and SE Low throughput HE 33 Mini Submarine Unit & EPS 301
260 Mini-Vertical Units are ideal Power Supply
modular units for purity confirmation High throughput, PhastSystem, PhastGel, & PhastGel DNA
applications. The small format delivers semi-automated Silver Staining Kit

fast and economical runs. For lower

throughput DNA analysis, HE 33 Mini
HE 33 Mini Submarine Unit Submarine Unit is perfectly suited.

When greater throughput is required,

PhastSystem™ is a self-contained unit
that runs precast small-format gels
and then stains with Coomassie™ blue
or PhastGel™ Protein Silver Staining Kit
within the same unit, with results
delivered in less than one hour. It is a
perfect complement to our ÄKTAFPLC™
System for reliable and flexible
protein purification.

SDS-PAGE of histidine-tagged glutathione

S-transferase purified from a cytoplasmic extract using
HisTrap™ Kit and ÄKTAFPLC. Analysis of sample fractions
performed on PhastSystem using PhastGel 10-15 and
PhastGel Protein Silver Staining Kit.

miniVE Vertical Electrophoresis System

6
Quantitative
determination
Analytical assessment of abundance

Protein Quantitative determination is used

when the absolute quantity of a nucleic
acid or protein band needs to be
established or compared to another
band for a ratio. Accurate sample
application and high resolution are of
prime importance in such cases.
When you need the greatest accuracy,
SE 600 Ruby SE 400 Vertical Unit, SE 600 Ruby or
Multiphor II Electrophoresis System are
the optimal tools.

Where convenient precast gels meet

your application needs, nothing beats
the modular flatbed Multiphor II SE 400 Vertical Unit
Electrophoresis System with ExcelGel
ExcelGel for SDS-PAGE precast gels for accurate sample Protein
with Multiphor II
Electrophoresis System loading and reproducibility.
Small format SE 250 or SE 260 Mini-Vertical Units or
Nucleic acid If you need the flexibility to cast your miniVE Vertical Electrophoresis System
& EPS 301 Power Supply
own gels and require a larger format,
SE 400 Vertical Unit and SE 600 Ruby Large format, SE 400 Vertical Unit, EPS 601
low throughput Power Supply
are ideal for your PAGE needs.
Large format, SE 600 Ruby, EPS 601 Power Supply,
medium throughput & MultiTemp III Thermostatic Circulator
GE Healthcare also supplies radiolabels
Large format, Multiphor II Electrophoresis System,
and fluorescent stains, such as Deep IEF or PAGE MultiTemp III Thermostatic Circulator, &
HE 33 Mini Submarine Unit Purple Total Protein Stain, to accurately EPS 3501 XL Power Supply with ExcelGel
quantitate your samples. Used together or Ampholine PAG plate precast gels
with Storm™ or Typhoon™ gel and blot
imaging systems, these detection
methods provide unsurpassed linearity Nucleic acid
and sensitivity.
HE 99X Max Submarine Unit Small format HE 33 Mini Submarine Unit
& EPS 301 Power Supply
Large format HE 99X Max Submarine Unit
& EPS 301 Power Supply

Visualization

Staining Deep Purple Total Protein Stain

Multiphor II Electrophoresis System, MultiTemp III Imaging Typhoon Variable Mode Imager
Thermostatic Circulator, EPS 3501 XL Power Supply, & Storm, gel and blot imaging system
Processor Plus

7
Quality assurance
and quality control
Samples analyzed for the presence of contaminants,
or confirm uniformity in your end products

In the field of electrophoresis, quality

assurance and quality control are
typically used to control the standard
of your end products. Quality assurance
provides feedback to improve your
manufacturing processes, while quality
control testing identifies defects in
products prior to shipping.

GE Healthcare’s electrophoresis product PhastSystem – semi-automated

portfolio has been designed to meet the flatbed electrophoresis system

needs of these quality assurance and

quality control applications.

PhastSystem is a semi-automated
flatbed electrophoresis system that can
run and detect 24 samples in one hour.
The system uses PhastGel gels, which
are precast and optimized for high
speed and reproducibility.

When larger format gels are required,

the Multiphor II Electrophoresis System
with ExcelGel precast SDS gels is a
reliable and convenient solution.
PhastGel for high reproducibility

Unrivalled reproducibility
Extensive quality control testing is an
integral part of PhastGel manufacture.
We typically run, analyze and record Protein
1000 test samples every week. These
high standards ensure the highest Large format Multiphor II Electrophoresis System,
reproducibility time and time again. No MultiTemp III Thermostatic Circulator, &
EPS 3501 XL Power Supply with ExcelGel
other electrophoresis gel is as reliable
High throughput, PhastSystem with PhastGel & PhastGel
and as well documented as PhastGel.
semi-automated Protein Silver Staining Kit

8
Clinical diagnostic
research
Screening of patient samples

Protein Clinical diagnostic research often

requires the use of electrophoresis
techniques. Many different types of
samples can be analyzed, the most
common being serum or blood. Typical
applications include running gels prior
TE 22 Mini Tank
to Western blotting, DNA analysis such
Transfer Unit as SSCP, and protein analysis to spot
the presence of specific markers.

Whether you are looking for the

Nucleic acid higher throughput analysis that only
PhastSystem can provide, or large-
format SDS-PAGE and Western blotting,
GE Healthcare has the solution for you.
PhastSystem is frequently referenced in
peer journals for its use in the field of
GenePhor DNA
Electrophoresis System diagnostic research. Its ease of use and SE 600 Ruby
fast, reliable results make it an excellent
choice for more commonly run samples,
and you can obtain PCR-SSCP results Protein
in less than one hour. Multiphor II
Electrophoresis System, in combination Small format SE 260 Mini Vertical Unit, TE 22 Mini Tank
with ExcelGel precast gels, offers a Transfer Unit, & EPS 301 Power Supply
large-format solution. Where higher Large format SE 600 Ruby MultiTemp III Thermostatic
Multiphor II Electrophoresis resolving capabilities and temperature Circulator, TE 77 Semi-Dry Blotting
System, MultiTemp III Unit & EPS 601 Power Supply
Thermostatic Circulator control are required for DNA
High throughput, PhastSystem with PhastGel
& Processor Plus applications, GenePhor™ DNA semi-automated and PhastTransfer
Electrophoresis System with GeneGel™
meets your needs.
Nucleic acid
SE 600 Ruby Standard Dual Cooled
Vertical Unit, EPS 601 Power Supply Medium throughput, GenePhor DNA Electrophoresis System
and TE 77 Semi-Dry Blotting Unit are medium format & EPS 1001 Power Supply with GeneGel
excellent for Western blotting detection Medium throughput, Multiphor II Electrophoresis System,
of agents such as HIV or the Borrelia large format MultiTemp III Thermostatic Circulator &
EPS 3501 XL Power Supply with ExcelGel
spirochete that causes Lyme disease. DNA Analysis Kit
GE Healthcare also provides a full range High throughput, PhastSystem with PhastGel
of products for efficient nucleic acid semi-automated
blotting, labeling, hybridization,
and detection.

9
Choose your route
to electrophoresis results
you can count on
Sample preparation

Quantitation

Electrophoresis

Protein analysis Nucleic acid analysis

2-D Analytical/Preparative Mutation detection

SDS-PAGE, IEF First Dimension Small <1000 bp Fragment analysis
Second Dimension Polyacrylamide PCR-SSCP, VNTR,
Medium 100 bp - 10 kb polyacrylamide, RAPD
Agarose
Vertical Flatbed Mini Large 10 kb - 10 Mb
Mini Mini Standard Pulsed field
Standard Standard Large

Detection

Staining
Stains/Dyes Blotting
Automated Gel Processor

Protein Nucleic acid Protein Nucleic acid

Gel drying UV visualization Tank Vacuum
Vacuum heat Semidry Tank
Air drying Membranes Membranes

Processing
Hybridization oven
UV Crosslinker
Automated membrane processor
Detection kits
Autoradiography film

Video documentation
Documentation
Image acquisition and analysis
and evaluation Photography

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madetoworktogether
GE Healthcare offers an extensive choice of gels, kits and reagents to complement
a wide range of electrophoresis instrumentation, all made to work together with your
application. Trusted by thousands of demanding scientists worldwide, our products
are backed by outstanding technical support, assuring you of results you can trust
every time.
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ÄKTAFPLC, Amersham Biosciences, Ampholine, Deep Purple, Ettan,

ExcelGel, GeneGel, Gene Navigator, GenePhor, HisTrap, Immobiline, Austria Latin America UK
IPGphor, LKB, Multiphor, MultiTemp, PhastGel, PhastSystem, Tel: 01/57606-1619 Tel: +55 11 3933 7300 Tel: 0800 616928
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of GE Healthcare Limited. Coomassie is a trademark of ICI plc.
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applications in life science research. The polymerase chain reaction
(PCR) is covered by patents owned by Roche Molecular Systems
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and F Hoffman-La Roche Ltd. A license to use the PCR process
for certain research and development activities accompanies Tel: 1 800 463 5800 Tel: 0165 580 410
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