Scribd
Upload
115 views

Overview of The QuantStudio 5

Uploaded by

Michael Okeke
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
115 views

Overview of The QuantStudio 5

Uploaded by

Michael Okeke
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 14

Overview of the QuantStudio™ 5 Real-Time

PCR System

The world leader in serving science


1
QuantStudio™ 5 Real-Time PCR Systems: The
Basics
• Touchscreen
• WiFi connectivity, enabling remote monitoring
• Low maintenance
• Factory calibrated for Applied Biosystems™ reagents

2
QuantStudio™ 5 Real-Time PCR Systems: The Basics

Touchscreen (stand-
alone capabilities,
PIN-protected user
accounts, and dye
calibration/RNaseP
functionality) USB ports

WiFi adapter
USB port for
port (optional use)
template upload
and data download Ethernet port :
RJ45
(10/100Mbps)
Motorized
block drawer
(controlled by Fuse cover
touchscreen)

Power port: 100/240


VAC
RS232 port (Service
only)

3
QuantStudio™ 5 Real-Time PCR System: The Basics

• VeriFlexTM Blocks
• 6 programmable zones
• Independent temperature control in each zone (more precise than
gradient)
• Can program at will, including multiple zones with same temp
• Great for optimization and also running multiple assays at the same time

60°C 61°C 62°C 63°C 64°C 65°C

4
Veriflex™ Blocks: Temperature Optimization

60oC 63oC 64oC 65oC

• Power SYBR™ Green Master Mix, with


primers designed using Primer Express ™
Software
• Secondary peak appears at 90°C when
60oC 61oC 62oC 63oC 64oC 65oC
annealing temperature goes above 63°C

TCF3 Assay
4 replicates for each temperature
Data courtesy of Verification and Validation

5
Multiplexing Capabilities

• OptiFlex™ System
with Bright White LED
• Six color unlocked
filter system
• Factory calibrated

*This filter set currently does not support any dyes supplied by Thermo Fisher Scientific

6
QuantStudio™ 5 Touch Screen

Open/Close
door

Help

Sign In or
Create a
Local User Settings:
Account - Calibration
- Runs
- Logs
Connection - Ship Prep
type

7
Instrument Local User Accounts

Create local pin- Connect to online


protected account Thermo Fisher
on instrument Cloud account

Optional but highly recommended for full capabilities

• Create individual accounts for multiple users


• PIN-protected accounts help keep protocols and data safe and stop
“accidental” run interruptions
• Instrument users can be designated as “Admin” or “Standard”
users
• First user defaults to “Admin” status but can create other Admins, as
needed

8
Create Local User Account and Link to Cloud

Enter User Name Set PIN


(and remember it!)

Why link to the Cloud?


The Cloud enables you to download run files from the
cloud and automatically upload them when complete

9
Admin and Standard User Accounts

Note: the first user with “Admin” status to connect to the


Cloud will be the Cloud Admin for that linked instrument

10
Monitor Progress During the Run

Time Remaining Thermal Protocol Status Live Amplification Curves

11
Recommended Calibration Schedule

Calibration type Frequency


ROI/Uniformity

Background Every two years

Dye
• After installing or moving the instrument
RNase P instrument
• As needed thereafter to confirm
verification run
instrument performance

12
QuantStudio™ Design and Analysis
Software

The world leader in serving science


13
QuantStudio™ Design and Analysis Software

• The QuantStudio Design and Analysis Software supports a


variety of analysis methods, including:
• Absolute Quantitation
• Standard Curve
• Relative Quantitation
• Relative Standard Curve
• Comparative CT (∆∆ CT)
• Multiplate ∆∆ CT Studies
• Presence/absence (Plus/Minus) assays with an internal positive
control
• Melt curve analysis
• Genotyping (including real-time amplification)
• Multiplate GEx analysis available online on the
QuantStudio Design and Analysis Cloud Software

14

You might also like