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Questions Tissue Engineering

Dr. Williams needs a scaffold to treat injuries in a football player's articular cartilage. Articular cartilage has limited self-repair abilities. Scaffolds provide 3D structural support for tissue development. Hydrogel scaffolds are most appropriate for cartilage. Alginate can be ionically crosslinked with calcium in under 30 minutes, making it suitable for Dr. Williams' needs. Physically crosslinked hydrogels can be reformed after deformation but chemically crosslinked hydrogels are more stable long-term. Stiffer hydrogels result from higher crosslinking density and polymer concentration.

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Mariz Martinez
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77 views

Questions Tissue Engineering

Dr. Williams needs a scaffold to treat injuries in a football player's articular cartilage. Articular cartilage has limited self-repair abilities. Scaffolds provide 3D structural support for tissue development. Hydrogel scaffolds are most appropriate for cartilage. Alginate can be ionically crosslinked with calcium in under 30 minutes, making it suitable for Dr. Williams' needs. Physically crosslinked hydrogels can be reformed after deformation but chemically crosslinked hydrogels are more stable long-term. Stiffer hydrogels result from higher crosslinking density and polymer concentration.

Uploaded by

Mariz Martinez
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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Tissue Engineering

Question 1: Dr. Williams explained his problem in treating the football player's injuries.
What are the characteristics of articular cartilage so that it requires treatment by tissue
engineering?
✓ Limited capacity for self-repair
– Handles minimum load in the body
– Contains massive blood vessel network
– Complex inorganic composition

Question 2: The primary element of tissue engineering, besides cells and growth factors, is a
scaffold. In this simulation, we will focus only on creating a scaffold.
What is the main purpose of scaffolds?
✓ Provide 3D structural support
– Cell division and gene expression regulator
– Cell source for tissue development
– Act as extracellular matrix and growth factors

Question 3: Numerous type of scaffolds for tissue engineering are available.


Which of these scaffolds do you think are appropriate as articular cartilage substitute?
✓ Hydrogel scaffold
– None of them
– Both hydrogel and solid scaffold
– Solid scaffold

Question 4: Crosslinks are bonds that link one polymer chain to another. Based on whether new
covalent bonds are formed, crosslinking methods can be divided into which two groups?
✓ Chemical and Physical
– Biological and Physical
– In-vitro and In-vivo
– Chemical and covalent

Question 5: Which ions can aid the crosslinking of alginate that is composed of mannuronic (M) and
glucuronic acid (G) residues?
✓ Calcium
– Hydrogen
– Sodium
– Potassium

Question 6: Which of these crosslinking methods generates relatively non-permanent crosslinks?


✓ Ionic crosslinking
– Enzymatic crosslinking
– Radical (UV) crosslinking
– Michael addition crosslinking

Question 7: Based on the data, which crosslinking method generates a very strong hydrogels?
✓ Michael-addition crosslinking
– Radical (UV) crosslinking
– Enzymatic crosslinking
– Ionic crosslinking

Question 8: Dr. Williams needs a scaffold for treating the injured football player. He needs to create
non-permanent scaffolds with excellent mechanical properties under 30 minutes.

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Which polymer and crosslinking method would you use the generate the hydrogel scaffold?
✓ Alginate and ionic-crosslinking
– Alginate and radical-crosslinking
– Hyaluronan and Michael-addition crosslinking
– PEG and radical-crosslinking

Question 9: Dr. Williams: Which class of hydrogel can eventually provide reversible gelling, and
can for example be reformed after shearing or melting?
✓ Physically crosslinked hydrogels
– Enzymatic crosslinked hydrogels
– Radical crosslinked hydrogels
– Chemically crosslinked hydrogels

Question 10: Dr. Williams: Which class of hydrogels is always long-term stable in medium?
✓ Chemically crosslinked hydrogel
– Ionic crosslinked hydrogels
– None of these
– Physically crosslinked hydrogel

Question 11: Dr. Williams: What will the hydrogels become when its elastic modulus gets higher?
✓ Stiffer
– More fragile
– Less stiff
– More elastic

Question 12: Dr. Williams: What will the hydrogels become when its elastic modulus gets higher?
✓ Stiffer
– More fragile
– Less stiff
– More elastic

Question 13: Dr. Williams: Which is NOT determine the mechanical properties of hydrogels?
✓ The site of transplantation
– The type of polymer
– Crosslinking density
– Polymer concentration

Question 14: Dr. Williams: What will the hydrogels become when its elastic modulus gets higher?
✓ Stiffer
– Less stiff
– More elastic
– Degrade faster

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