Scribd
Upload
111 views

GPAT Biotechnology Syllabus

This document outlines the syllabus for a GPAT Biotechnology course. It covers 6 major topics: 1) Plant Cell and Tissue Culture, 2) Animal Cell Culture, 3) Fermentation Technology and Industrial Microbiology, 4) Recombinant DNA Technology, 5) Processes and Applications of Recombinant DNA Technology, and 6) Biotechnology Derived Products. Some key areas discussed include plant and animal cell structure and culture, gene transfer techniques, restriction endonucleases, DNA cloning, PCR applications, recombinant protein production in E. coli and other systems, downstream processing, and validation studies for detecting contaminants in biotechnology products.

Uploaded by

kumar Harsh
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
111 views

GPAT Biotechnology Syllabus

This document outlines the syllabus for a GPAT Biotechnology course. It covers 6 major topics: 1) Plant Cell and Tissue Culture, 2) Animal Cell Culture, 3) Fermentation Technology and Industrial Microbiology, 4) Recombinant DNA Technology, 5) Processes and Applications of Recombinant DNA Technology, and 6) Biotechnology Derived Products. Some key areas discussed include plant and animal cell structure and culture, gene transfer techniques, restriction endonucleases, DNA cloning, PCR applications, recombinant protein production in E. coli and other systems, downstream processing, and validation studies for detecting contaminants in biotechnology products.

Uploaded by

kumar Harsh
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 3

GPAT Biotechnology Syllabus

I. Plant Cell and Tissue Culture

Structure of plant cell, DNA, Genes and chromosomes.

A.. Cell and tissue culture,

a. Requirements.
b. Callus culture, suspension culture, batch culture.
c. The concept of somatic hybridization, somatic embryogenesis.

B. Processes and applications,

a. Isolation and immobilization of enzymes and plant cells and application.


b. Protoplast and cell fusion.
c. Germplasm conservation.
d. Production of secondary metabolites by plant tissue culture.
e. Gene transfer techniques.

II. Animal Cell Culture

Introduction to animal cell culture, medium used in ATC. Use of FCS, primary
culture, secondary culture, cell line. Cloning: concept and application with
technical hurdles. Transgenic animals as a source of food, organs and tissues,
concept of xeno transplant.

III. Fermentation Technology and Industrial Microbiology

1. Fermentation as a biochemical process, types of fermentation.


2. Fermenter – working and construction, accessory components, modification.
3. Fermentation monitoring and in situ recovery of products.

IV. Recombinant DNA Technology

Basic concepts

a) Introduction.
b) Role of a restriction endonuclease, DNA ligase, DNA polymerase,
Reverse transcriptase.
V. Process and Applications

a) Constructing Recombinant DNA molecules.

DNA Clones sources of DNA for cloning.


DNA vectors, role of expression vectors.
Host cell for recombinant work.
Method for screening and selecting transformants.
Expression of foreign genes.
Uses of recombinant DNA.

b) PCR and applications.

Human gene therapy concept and applications.

c) Drug delivery systems in gene therapy.

VI. Biotechnology Derived Products

a) Sources and upstream processing.


Introduction.

Escherichia coli as a source of recombinant, therapeutic protein.


Additional production systems : Yeast, Fungal production systems,
Transgenic animals, Transgenic plants, Insects cell-based systems
Upstream processing.

b) Downstream processing.

Product analysis : Introduction, Protein-base contaminant, Removal of an


altered form of the protein of interest from the product stream.
Determination of protein concentration.

c) Immunological approaches to detection of contaminant, Endotoxin and other

 pyrogenic contaminants.

 Pyrogen detection.

 DNA as a contaminant.
 Microbial and viral contaminant.

 Viral assays.

 Miscellaneous contaminants.

 Validation studies.

d) Production and purification of recombinant proteins like, Insulin, Growth


hormones, somatostatin, interferons, only examples of recombinant blood products.

You might also like