VIETNAM NATIONAL UNIVERSITY – HO CHI MINH CITY

HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY

FACULTY OF CHEMICAL ENGINEERING

SUBJECT:  LABORATORY OF FOOD ANALYSIS A

REPORT OF SAMPLE PREPARATION AND SAFETY

Class: CC01

Group: 07

Instructor: Assoc. Prof. Dr. NGUYEN THI LAN PHI, PhD

Group members:

Huỳnh Diệp Mỹ Phụng 2011869

Đặng Thụy Huỳnh Như 2011771

Nguyễn Cao Ngọc Quý 2053389

1. List the tools/equipments used in each Unit:
Unit 1: Pipet calibration and standard solution preparation.
Experiment 1: Pipet calibration
● Sample: Stem cells
● List of tools/ equipment: Pipette, pipette tips, beaker, thermometer, a balance
(need to be specific to micrograms to calibrate micropipettes with a maximum
of 1μL) weight boats, chamber.
● List of chemicals: Distilled water, ethanol (kill contaminants), stem cells.

Experiment 2: Standard solution

● Sample: Hydrochloric acid
● List of tools/equipment: Pipette, volumetric flask, burette, dropper.
● List of chemicals: Hydrochloric acid, distilled water, NaOH solution.

Unit 2: Determination of total acidity by volumetric titration and potentiometric

titration
● Food Sample: Clove of lemon
● List of chemical:
- Volumetric titration: Distilled water, phenolphthalein, sodium hydroxide
● List of tools/ equipment:
- Volumetric titration: Conical flask, beaker, burette, pipette, dropper
- Potentiometric titration: An electric burette, a sensor, beaker

Unit 3: Structural analysis of food sample: TPA analysis

● Food sample: Peanut butter
● List of tools/ Equipment: Texture Analyzer, a plate, a probe

Unit 4: Determination of moisture content of food sample.

● Food sample: Milk powder
● List of Chemical: Toluene, distilled water
● List of tools/ equipment: Graduated cylinder, weighing papers/ pans, beaker,
crucible, desiccator, plastic gloves, spatula, tray, volumetric pipette, hot plate,
glass stirring rod, analytical balance, microwave drying oven, rapid moisture
analyzer.

Unit 5: Determination of amino acids by thin layer chromatography (TLC)

● Food sample: Salt
● Tools/ Equipment: Glass plate, developing chamber with a cover, thin-layer
spreader, marked pen, ruler, capillary tubes.
 ● Chemicals: Silica gel, ninhydrin solution in acetone, standard amino acids of
0.02M in water.

Unit 6: Determination of reducing sugar content by UV-VIS:

● Food sample: Corn syrup
● Tools/Equipment: Spectrophotometer, volumetric flask, pipette, test tube rack,
test tubes, spatulas, weighing paper, analytical balance, pH meter, water bath,
adjustable volume pipettors with tips.
● Chemicals: Glucose standard solution, diluted sulfuric acid, 0.1M acetate
buffer (sodium acetate dissolved in water), glucose test solution (glucose
oxidase + horseradish oxidase o-dianisidine.2HCl in the 0.1 M acetate buffer).
 2. List the chemicals used in each Unit and describe the Hazard classification of the chemicals:
Unit Chemicals Hazards

CAS no. Labeling

Signal Pictogram Hazard statements Precaution statements

word

1 Ethanol A405-20; Danger Highly flammable liquid and Obtain special instructions before
A405F-1 GAL; vapor use. Do not handle it until all
A405P-4 Causes serious eye irritation safety precautions have been read
Suspected of causing cancer
and understood. Use personal
Suspected of damaging
fertility or the unborn child protective equipment as required.
Wash face, hands and any exposed
skin thoroughly after handling
Wear eye/face protection. Do not
breathe
dust/fume/gas/mist/vapors/spray.
Do not eat, drink or smoke when
using this product. Keep away
from heat/sparks/open flames/hot
surfaces.
-No smoking. Keep the container
tightly closed. Ground/bond
container and receiving equipment
Use explosion-proof
electrical/ventilating/lighting.
 equipment. Use only non-sparking
tools. Take precautionary
measures against static discharge.

1 Hydrochloric 7647-01-0 Danger May be corrosive to metals. Keep only in original packaging.
acid Causes severe skin burns and Avoid breathing mist or vapors.
eye damage. Use only outdoors or in a
May cause respiratory well-ventilated area.
irritation. Wear protective gloves/ protective
clothing/ eye protection/ face
protection.
In case of skin contact: Take off
immediately all contaminated
clothing. Rinse skin with water.
Eye contact: Rinse cautiously with
water for several minutes. Remove
contact lenses, if present and easy
to do. Continue rinsing.

2 Sodium Danger May be corrosive to metals. Causes eye burns. May cause
hydroxide Causes severe skin burns and blindness. May cause chemical
eye damage. conjunctivitis and corneal damage.
Causes skin burns. May cause
deep, penetrating ulcers of the
skin. May cause severe and
permanent damage to the digestive
tract.
Personal Protective Equipment:
 Eyes: Wear chemical splash
goggles and face shield.
Skin: Wear butyl rubber gloves,
apron, and/or clothing.
Clothing: Wear appropriate
protective clothing to prevent skin
exposure.

2 Phenolphthalein 77-09-8 Warning Causes skin irritation. Do not handle it until all safety
Suspected of causing genetic precautions have been read and
defects. understood.
May cause cancer. Wash skin thoroughly after
Suspected of damaging handling.
fertility. Wear protective gloves/ protective
clothing/ eye protection/ face
protection.
Skin contact: Wash with plenty of
water.
If exposed or concerned: Get
medical advice/ attention.
If skin irritation occurs: Get
medical advice/ attention.

4 Toluene Danger Highly flammable and is

harmful if inhaled
5 Ninhydrin Danger Highly flammable, harmful -Keep away from heat, hot
solution in if swallowed, can cause surfaces, sparks, open flames and
acetone serious eye damage, may other ignition sources. No
cause respiratory irritation, smoking.
skin irritation, may cause -Keep the container tightly closed.
drowsiness or dizziness. -Avoid breathing
dust/fume/gas/mist/vapors/spray.
-In case of eye contact: Rinse
cautiously with water for several
minutes. Remove contact lenses, if
present and easy to do. Continue
rinsing.
-In case of skin contact: Remove
contaminated clothing. Wash skin
with water and mild soap. If
irritation persists, seek medical
attention.
-In case of burns: Flush with water
until pain ceases. Do not remove
clothes if attached to skin. If
medical attention is needed, flush
the inflicted area, until medical
doctor is present.
-Wear protective gloves/protective
clothing/eye protection/face
protection.

5 Silica gel 112926-00-8 Not a hazardous substance or If inhaled after inhalation: fresh
mixture according to air.
 Regulation In case of skin contact: Take off
immediately all contaminated
clothing. Rinse skin with water/
shower.
If swallowed After swallowing:
make victim drink water (two
glasses at most). Consult doctor if
feeling unwell.

6 Concentrated 7664-93-9 Danger May be corrosive to metals. Keep only in original packaging.
sulfuric acid Causes severe skin burns and Wear protective gloves/ protective
eye damage. clothing/ eye protection/ face
protection.
Skin contact: Take off immediately
all contaminated clothing. Rinse
skin with water.
If inhaled: Remove person to fresh
air and keep comfortable for
breathing. Immediately call the
doctor.
Eye contact: Rinse cautiously with
water for several minutes. Remove
contact lenses, if present and easy
to do. Continue rinsing. Wash
contaminated clothing before
reuse.
6 Acetic acid 64-19-7 Danger Flammable liquid and vapor. Keep away from heat, hot
Causes severe skin burns and surfaces, sparks, open flames and
eye damage. other ignition sources.
No smoking.
Wear protective gloves/ protective
clothing/ eye protection/ face
protection.
If swallowed: Rinse mouth. Do not
induce vomiting.
Skin contact: Take off immediately
all contaminated clothing. Rinse
skin with water.
Eye contact: Rinse cautiously with
water for several minutes. Remove
contact lenses, if present and easy
to do. Continue rinsing.
Immediately call doctor.

6 Acetate Buffer Warning May cause an allergic skin Wear protective gloves.
reaction.

6 O-dianisidine 20325-40-0 Danger Harmful if swallowed. Do not breathe dust.

2HCl Causes severe skin burns and Wear protective gloves/ protective
eye damage. clothing/ eye protection/ face
May cause cancer. protection.
If swallowed: Call doctor if you
feel unwell.
Skin contact: Take off immediately
all contaminated clothing. Rinse
skin with water.
If inhaled: Remove person to fresh
air and keep comfortable for
breathing. Immediately call doctor.
Eye contact: Rinse cautiously with
water for several minutes. Remove
contact lenses, if present and easy
to do. Continue rinsing.
3. Write down how to prepare 100.0 mL of 0.100N NaOH and 0.100M NaOH
solutions from 98.5% NaOH standard solution.
a. For 0.100N NaOH solution
0.100 × 100.0 × 40
𝑉 𝑠𝑡𝑜𝑐𝑘 𝑠𝑜𝑙𝑢𝑡𝑖𝑜𝑛 = 98.5 ×2.13 𝑥 10
= 0. 190 𝑚𝑙
1. Use micropipette to take 0.2 ml stock solution to 100ml volumetric flask, then
add H2O to meet the red line in the 100ml volumetric flask.
2. Shake well.
b. For 0.100M NaOH solution
0.100 × 100.0 × 40
𝑉 𝑠𝑡𝑜𝑐𝑘 𝑠𝑜𝑙𝑢𝑡𝑖𝑜𝑛 = 98.5 ×2.13 𝑥 10
= 0. 190 𝑚𝑙
1. Use pipette to take 1 ml stock solution to 100ml volumetric flask, then add
H2O to meet the red line in the 100ml volumetric flask.
2. Shake well.

4. Write down the procedure to calibrate the pipette 5.0 mL

Step 1: Take distilled water in a beaker and record its temperature.
Step 2: Gather your pipette and the correct tips based on both the small and large
volumes that the pipette can dispense.
Step 3: Place a weight boat on a balance that can accurately weigh in the microgram
range, and set it to zero after closing the balance door.
Step 4: Pre-rinse the tip by aspirating and dispensing the set volume 3 times and push
fully to remove any remaining liquid.
Step 5: Aspirate the calibration volume without bubble formation and dispense the
liquid slowly into the weight boat. Then, record the weight on the balance and repeat
the process 3 times.
Step 6: Wait one second and withdraw the tip from the liquid.
Step 7: Wipe any droplets away from the outside of the tip using a kimwipe.
Step 8: Place the end of the tip against the inside wall of the plastic cup at an angle of
10-40°C.
Step 9: Press the push-button smoothly to the first stop. Wait one second, change to a
new site and press the push-button on the second stop.
Step 10: Keep the push-button pressed to the end, remove the pipette by drawing the
tip along the inside 2 surfaces of the plastic cup and release the push button.
Step 11: Close the door of balance and record the value on the balance display after it
has stabilized.
Step 12: Repeat step (6) to (12) 14 times (the weight display of balance should be
adjusted to zero every time)
Step 13: Eject the tip.
Step 14: Pipette should be calibrated every three months routinely.