SEMESTER-VIII i CHM-612: ELECTRONICS AND MASS SPECTROMETRY Credit Hours: (3+0) (A) Electronics Introduction: conductors, insulators, charge, electric field, potential, potential difference, current, electron flow, conventional current, direct ; and alternating current, hole charge, resistance and resistors, open and closed circuits. 4 Resistors and resistance: Color coding, factors affecting resistance, Ohms law, concept of power and units. - Circuit Symbols and circuits: Symbols of components of circuits, series circuits and parallel circuits, combination of series and paralle} circuits, Kirchoff Voltagé law, Kirchoff Current law, Wheat stone bridge, fuse and its types. Methods of solving circuits: Mesh circuit analysis, method of branch current, Node voltage analysis. 4 Capacitors: Series and parallel capacitors and circuit capacitor reactance. Ohms law as applied to capacitance current. a Electricity and magnetism: Definition and units of magnetic flux, flux — density, Induction, permeability, relay, Classification of magnetic — material. Magnetomotive force, ampere turns, field intensity, Ohms law for magnetic currents. Magnetic field around a conductor, right hand rule, magnetic polarity, motor action, induced current, Faraday’s law. Generation of AC current: Methods and princi A forms, Induction by AC currents, mutual inductance, ene ee a reactance, RC and L/R time constant AC circuits. Filters, High pass Low pass filters, RC band pass, Band stop filters, 2 Semiconductors: p-n junctions, Applications, worki ing of transistors _ i and their uses. Nae hs : ee 4 Binary insoles nie ae ae ee pepdint. ee id Boolean Algebra; Binary to decimal hy aoe are Come: ; (ape hee NOT a ON ala Aa tari sg : Combination of the above circuits, read out devices,oo: signals gital to analogue conversion, In-jj Components of personal ¢ : tor, controller. Signal to noise ratio: De Finition and seope B) Mass Spectrometry Definitions: mass, mass-to-charge ratio, resoluti distributions. Theoretical principles, Tonization pier Y, isotope 'MALDI. Instrument components: Mass analyzers: secto , Cl, FAB, flight, quadrupole, ion traps MS/M : rs, time-of- Sand tande} i ; i min: Behaviour of various classes of compounds in mass spect omer eel _ interpretation of mass spectra and applications to chemical analysis, BOOKS RECOMMENDED Pe Grob B “Basic Electronics” 8" Ed (1997) McGraw Hill Petruzella FD “Essentials of Electronics” 2™ Bq (1999) Mc Graw Hill ) 3. Skoog AD, Leary JJ “Principles of Instrumental Analysis” 4" Ed (1992) Saunders College Publishing 4. Barker J and Ando DJ “Mass Spectrometry” Analytical Chemistry by Open Learning 2" Ed (1998) 5. Hoffmann E and Stroobant V “Mass ‘Spectrometry: Principles and Applications” Wiley 2" Ed (2001) : 6. Silverstein RM, Webster FX and Kiemle D “Spectrometric Adentification of Organic Compounds” 7" Ed (2005) John Wiley and Sons. ; CHM-622: ELECTROANALYTICAL CHEMISTRY AND INTRODUCTION TO ANALYTICAL SEPARATION Credit Hours: (3 + 0) Double Layer: eae inte e, OHP and IHP, contact pt 5 differences across metal solution interfaces, outer ce, electrochemical potential pillary thermodynamics,transfer proc bsence of electrical field, the overpotential, Butler- /olmer Equation, the idea of equilibrium exchange current density, the — symmetry factor, high filed and low filed approximations, Tafel equation and Nernst equation. Introduction to electroanalytical techniques, review of electrochemical cells, cell potentials, electrode potentials, currents in electrochemical cell, types of electroanalytical methods. trode Kinetics: Electrochemical devices, charge the presence and absen Non-Potentiometric Electroanalysis : Polarography and voltammetry, electrodes, polarographic principles, excitation signals in voltammetry, linear scan voltammetry, pulse polarographic and voltammetric methods, stripping methods, and cyclic voltammetry. (B) Introduction to Analytical Separations Introduction to various separation techniques (precipitation, distillation, extraction, lypholization and chromatography) High Performance Liquid Chromatography Instrument, stationary phases, mobile phases, applications of HPLC Gas Chromatography Instrument, stationary phases, mobile phases, GC columns, types of detectors, application of GC. HPLC columns, “BOOKS RECOMMENDED 6. Bockris JOM and Reddy AKN “Moder Electrochemistry” Vol I 2 and II , 4 Ed. (2003) Plenum Press, London. 5 7. Bockris JOM and Draziac D “Electrochemical Science” (1972) a Taylor and Fracis, London i y 8. Bockris JOM, Bonciocat N and Guttmann F “An introduction to Electrochemical Science” (1974) Wykeham Publication, London Bockris JOM and Fredlein RA “A Workbook of Electrochemistry” (1973) Plenum Press,New York — + < “ Skoog AD, Holler FJJ and Nieman TA “P ysis” 5" Ed (1997) Saunders College Pula : -622.1: PHARMACE , RY Oe cc UTICAL CHEMISTRY (Q ~ Credit Hours: (3+0) firs sformation of Chemicals into Drugs Physiochemical Properties In Relation To Biological Acti prenis between drug administration and ae iain steele partition coefficient and drug-receptor interactione og — Factors Influencing Dosa: absorption of drugs, thei effects, MIC values etc. ‘ge Formations: Disirite it pre-requisites, Effect and Drug-drug interac gration, dissolution and tive blood level, Placebo tions 4 Dmg Metabolism: Factors influencing metabolism of drugs. Site of metabolism, Metabolic changes in GI tract, Types of metabolic Teactions and Reactions based on functional groups. oo Structure-Activity Relationship (SAR): Chemical structure in relation _ - to biological activity of molecules, stereochemical factors, prodrugs, isosters and pharmacophore groups. 2 Synthesis, physical and chemical properties, mode of action, SAR studies and toxicity of the following drugs Sulfa drugs: Sulfa drugs*in current therapy (human and veterinary use). Drugs -acting on NIDDM: Sulfonylureas. Antibiotics: Penicillin and ___ Telated compounds, cephalosporins, tetracyclines chloramphenicol and * quinolones. Antiviral agents and antineoplastic agents. Histamines and antihistamines: H, and H,-receptor antagonists, development of H; Teceptors and proton puntp inhibitors. Anti-malarial drugs, Analgesics (peripheral as well as centrally acting), local anesthetics Recent trends in drug development: Quantitative eee eo relationship (OSAR), computer models and stimulations with examp! including a case study of at least one drug. BOOKS RECOMMENDED 16 Burger A “Medicinal Chemistry” an ie sey Interscience York great Burger A “Medicinal Chemistry” 5" Ed Wi es 2. Wiley Interscience New York i Jenkins GL et al. “The Chemistry of Organic Medicinal P Wiley Interscience New York . 6. Clarks ECG “Isolation and Identification of Drugs” Vol I a The Pharmaceutical Press, London “ CHM-632: SPECTROSCOPY -II + Credit Hours: (3+0) UV-Visible Spectroscopy F ‘a 5 Energy transitions in molecules, types of electronic transitions in” molecules (n- %,-"- * 9-o* etc.), selection rules, Beer’s Lambert law” and electronic spectra, explanation of bathochromic, hypsochromi¢ 7 shifts, fundamentals of the optical components of spectrometers, ” radiation sources, monochromators based on Czerny Turner mounting, and echelle gratings, detectors, photometric accuracy and high 7 precision techniques, spectrophotometric errors, analysis of | multicomponent analysis and derivative spectroscopy, diode - array © spectrophotometers. Infrared Spectrophotometry j Interaction of infrared radiation with molecules, types of molecular vibrations, selection rules, instrumentation, optical materials, sources, detectors used in IR spectroscopy. Interfometric (Fourier Transform) — spectrophotometers, calibration and standardization and preparation of samples for the analysis of solids, liquids and gases E Luminescence Spectrometry (Fluorescence, Phosphorescence &% Chemiluminescence): Introduction, types of luminescence, energy levels in luminescent’ imolecules, Process involving excited states i.e. absorption, vibrati . telaxation, internal conversion, fluorescence, intersystem crossing 4 phosphorescence. Instrumentation for fluorescence measurements a -phosphoresce measurements, excitation and emission spt luminescence efficiency and factors affecting it, post and pre ? eoeees _ telationship between lumine: ie jerpueatonsers, atomization cells (flames a, Tree tion spectroscopy, flame omiasaee Sraphite furnag their control, cold vapor for mercury ane hniques for trace analysis of metals, c jum lamp, Smith Hieftje technique an () Emission spectroscopy Use of Grotrian diagram, the Phenomenon of flame emissi jnterferences encountered in flame emission, emission g ie and ~ pased on plasma sources and ICP spectrometer, simultaneous pare ‘ofelements by ICP alysis . _ BOOKS RECOMMENDED 1. “Skoog AD, Holler FJJ, Nieman TA “Principles of Instrumental ~ Analysis” 5" Ed (1997) Saunders College Publishing, New York 2. Willard HH, Merrit LL, Dean JA and Settle FA “Instrumental Methods of Analysis” 7 Bd (1 988) Wadsworth Publishers 3 Christian GD, O’Reilly JE “Instrumental Analysis” 2™! Ed (1986) Allyn and Bacon, Inc : 4 Rousseac F and Rousseac’ A “Chemical Analysis-Modem Instrumentation Methods and Techniques” (2000) Wiley a 5. Braun RD "Introduction to Instrumental Analysis sep) < Graw Hill, New York i 3 6 Strobel HA "Chemical Instrumentation: Se Approach to _ Ins 1 Analysis" (1989) Wiley, New Yor eee cx sca EM “Fundamentals of Molecular Spectroscopy” (1994) Tata Mc Graw Hill a a ALYSIS HM-642: CLASSICAL METHODS OF CHEMICAL ANAL it Hours (0+3) (Laboratory Course) ane samples using the analysis of real Sam gues “rnethods of analysis. The Neutralization titrations, recox ' chromatography and gravimetry. 1 of acetic myDetermination of copper by iodometry. Determination of ascorbic acid in vitamin ‘tablets. Determination of sodium hypochlorite in commercial ble iodometry — : Winkler titration for the determination of oxygen in water : Separation of two cations by anion exchange chromatography. Total cations in water by cation exchange chromatography. ~ Gravimetric determination of copper as CuO. : Homogenous precipitation of Fe as Fe,0s. ; Other new experiments will be incorporated depending upon the availability of reagents and apparatus. Weekly Seminars and Student Presentations: Based on theoretical _ and practical topics related to titrimetry, gravimetry, laboratory satel | and pees usage and calibration of laboratory glassware. Ba RECOMMENDED ’ Laitinen, HA “Chemical Analysis: An Advanced Text aa Reference” (1960) Mc Graw Hill, New York e 2. Harris D “Quantitative Chemical Analysis” 5" Ed (1998) WH : Freeman and Company 3. Mendham J, Denney RC, Barhes JD and Thomas MJK “voaat Quantitative Analysis” oe Ed (2000) Prentice Hall 4. Mendham J, Dodd D and Cooper D “Classical Methods” VolI II (1987) (ACOL series) London CHM-652 CLINICAL METHODS OF ANALYSIS ENVIRONMENTAL ANALYTICAL CHEMISTRY Credit Hours: (0 + 3) (Laboratory Course) ‘ - Quantitative analysis of Na‘, K*, Ca’ *, Cl and inorgat phosphorus in serum. 2 4 ¢ Analysis of serum glucose by o-toluidine and “copper method. ‘i Analysis of NPN i.e. creatinine, utea-N and uric acid i Determination of serum cholesterol concentration, Analysis of serum iron and TIBC. _ Determination of protein (total, eee wegenet Ghent eprinn ; ment lust, analysis, mination of organic pollutants jn — matography, analysis of toxic heavy metals in samples by atomic absorption or emission Spectroscopy, plant mate by | Nobert and Tietz W “Textbook of Clinical Chemistry” 2nd Ed (1994) WB Saunders Company, Philadelphia 2. Kaplan A and Szabo LL “Clinical Chemistry: Interpretation and 3 . Techniques” 3“ Ed (1988) Lea and Febiger, Philadelphia Bender GT “Principles of Chemical Instrumentation” (1987) WB Saunders Company, Philadelphia