Certiied

Certiicate of Analysis Reference

SUPELCO 37 COMPONENT FAME MIX
Material
Product ID CRM47885
Lot LRAB8645
Expiration Date April 2021
Manufacturing Date May 2018
Storage Conditions Freeze
Solvent/Matrix METHYLENE CHLORIDE
1,4 Elution Raw Material
Certiied Raw Material
Analyte Units Purity,% order Lot CAS
Value

METHYL BUTYRATE µg/ml 405 ± 1.83 99.9 MKBS9252V 623-42-7

1

METHYL HEXANOATE µg/ml 405 ± 2.05 99.9 MKBZ3038V 106-70-7

2

METHYL OCTANOATE µg/ml 405 ± 4.34 99.9 MKCB1249V 111-11-5

3

METHYL DECANOATE (CAPRATE) µg/ml 405 ± 4.53 99.9 LC04160 110-42-9

4

METHYL UNDECANOATE µg/ml 173 ± 2.35 86.6 BCBS9431V 1731-86-8

5

METHYL LAURATE µg/ml 405 ± 4.77 99.9 11963 111-82-0

6

METHYL TRIDECANOATE µg/ml 203 ± 1.25 99.6 BCBQ2899V 1731-88-0

7

METHYL MYRISTATE µg/ml 404 ± 2.58 100 LC17239 124-10-7

8

MYRISTOLEIC ACID METHYL µg/ml 202 ± 2.57 100 SLBR18858V 56219-06-8

9
ESTER (Methyl myristoleate)
METHYL PENTADECANOATE µg/ml 202 ± 1.33 99.9 BCBP0333V 7132-64-1
10

CIS-10-PENTADECENOIC ACID µg/ml 204 ± 1.33 99.0 U38MJ23C 90176-52-6

11
METHYL ESTER
METHYL PALMITATE µg/ml 612 ± 4.16 99.0 LC17089 112-39-0
12

METHYL PALMITOLEATE (METHYL µg/ml 204 ± 1.33 99.0 LC20929 1120-25-8

13
CIS 9-HEXADECENOATE)
METHYL HEPTADECANOATE µg/ml 101 ± 0.68 50.6 LC05285 1731-92-6
14

CIS-10-HEPTADECENOIC ACID µg/ml 204 ± 1.39 99.0 SLBR8122V 75190-82-8

15
METHYL ESTER (Methyl
cis-10-heptadecenoate
)
METHYL STEARATE µg/ml 408 ± 3.1 99.1 LC13654 112-61-8
16

TRANS-9-ELAIDIC ACID METHYL µg/ml 202 ± 1.43 99.9 LC15380 1937-62-8

17
ESTER (Methyl elaidate)
CIS-9-OLEIC ACID METHYL ESTER µg/ml 407 ± 2.94 99.3 MKBZ8013V 112-62-9
18
(Methyl oleate)
METHYL LINOLEATE µg/ml 203 ± 1.51 99.7 BCBS1588V 112-63-0
19

LINOLELAIDIC ACID METHYL µg/ml 202 ± 1.46 100 SLBV6131 2566-97-4

20
ESTER (Methyl linolelaidate)
METHYL ARACHIDATE µg/ml 408 ± 3.01 99.0 0000004248 1120-28-1
21

GAMMA-LINOLENIC ACID METHYL µg/ml 202 ± 1.50 100 LC17723 16326-32-2

22
ESTER (Methyl γ-linolenate)

2931 Soldier Springs Rd. Laramie, Wyoming 82070 USA

307-742-5452
[email protected] www.sigma-aldrich.com
Page 1 of 5
 LotLRAB8645
Expiration DateApril 2021
Manufacturing DateMay 2018
Storage ConditionsFreeze
Solvent/Matrix METHYLENE CHLORIDE
METHYL CIS-11 EICOSENOATE µg/ml 202 ± 1.50 100 MKCF0789 2390-09-2
23

METHYL LINOLENATE µg/ml 202 ± 1.50 99.9 LC03119 301-00-8

24

METHYL HENEICOSANOATE µg/ml 203 ± 1.56 99.6 LC08970 6064-90-0

25

CIS-11,14-EICOSADIENOIC ACID µg/ml 200 ± 1.47 99.9 LC23224 2463-02-7

26
METHYL ESTER
METHYL BEHENATE µg/ml 405 ± 2.90 99.7 LC03090 929-77-1
27

CIS-8,11,14-EICOSATRIENOIC µg/ml 163 ± 1.51 79.5 U81MF27C 21061-10-9

28
ACID METHYL ESTER
METHYL ERUCATE µg/ml 203 ± 2.76 99.4 BCBR3848V 1120-34-9
29
(CIS-13-DOCOSENOATE)
(Methyl cis-13-docosenoate)
CIS-11,14,17-EICOSATRIENOIC µg/ml 150 ± 3.73 75.0 LC20739 55682-88-7
30
ACID METHYL ESTER
METHYL TRICOSANOATE µg/ml 165 ± 1.30 82.6 LC06065 2433-97-8
31

METHYL µg/ml 202 ± 1.63 100 LC15184 2566-89-4

32
CIS-5,8,11,14-EICOSATETRAE
NOIC ( Methyl arachidonate)
CIS-13,16-DOCOSADIENOIC µg/ml 204 ± 1.57 99.0 U-69M-AU9-B 61012-47-3
33
ACID METHYL ESTER
METHYL LIGNOCERATE (Methyl µg/ml 405 ± 5.35 99.9 SLBV3084 2442-49-1
34
tetracosanoate)
METHYL µg/ml 140 ± 1.45 70.0 LC27070 2734-47-6
35
CIS-5,8,11,14,17-EICOSAPEN
TAENOATE
METHYL NERVONATE (Methyl µg/ml 204 ± 1.68 99.0 U88MJA11C 2733-88-2
36
cis-15-tetracosenoate)
ALL µg/ml 134 ± 1.57 67.2 LC18247 2566-90-7
37
CIS-4,7,10,13,16,19-DOCOSA
HEXAENOATE

Page 2 of 5
 Certiied
Certiicate of Analysis Reference
SUPELCO 37 COMPONENT FAME MIX
Material
Product ID CRM47885
Lot LRAB8645
Expiration Date April 2021
Manufacturing Date May 2018
Storage Conditions Freeze
Solvent/Matrix METHYLENE CHLORIDE

12

6 8 18
4
3 16
21
27 34
2

1 5 7 9 10 11 13 15 19 20 22 24 26 2830 33 35
17 2325 2931 36 37
32

14

2931 Soldier Springs Rd. Laramie, Wyoming 82070 USA

307-742-5452
[email protected] www.sigma-aldrich.com
Page 3 of 5
 LotLRAB8645
Expiration DateApril 2021
Manufacturing DateMay 2018
Storage ConditionsFreeze
Solvent/Matrix METHYLENE CHLORIDE

Order or Elution
1. Methyl Butyrate
2. Methyl Hexnoate
3. Methyl Octanoate
4. Methyl Decanoate (Caprate)
5. Methyl Undecanoate
6. Methyl Laurate
7. Methyl Tridecanoate
8. Methyl Myristate
9. Myristoleic Acid Methyl Ester
10. Methyl Pentadecanoate
11. Cis-10-Pentadecenoic Acid methyl Ester
12. Methyl palmitate
13. Methyl Palmitoleate (Methyl Cis 9-Hexadecenoate)
14. Mehtyl Heptadecanoate
15. Cis-10-Heptadecenoic Acid Methyl Ester (Methyl cis-10heptad)
16. Methyl Stearate
17. Trans-9-Elaidic Acid Methyl Ester (Methyl elaidate)
18. Cis-9-Oleic Acid Methyl Ester
19. Methyl Linoleate
20. Linolelaidic Acid Methyl Ester (Methyl Linolelaidate)
21. Methyl Arachidate
22. Gamma-LinolenicAcid Methyl Ester (Methyl gamma-linolenate)
23. Methyl Cis-11 Eicosenoate
24. Methyl Linolenate
25. Methyl Heneicosanoate
26. Cis-11,14-Eicosadienoic Acid Methyl Ester
27. Methyl Behenate
28. Cis-8,11,14-Eicosatrienoic Acid Methyl Ester
29. Methyl Erucate (Cis-13-Docosenoate) (Methyl cis-13-docosenoa)
30. Cis-11,14,17-Eicosatrienoic Acid Methyl Ester
31. Methyl Tricosanoate
32. Methyl Cis-5,8,11,14-Eicosatetraenoic (Methyl Arachidonate)
33. Cis-13,16-Docosadienoic Acid Methyl Ester
34. Methyl Lignocerate (Methyl Tetracosanoate)
35. Methyl Cis-5,8,11,14,17-Eicosapentaenoate
36. Methyl Nervonate (Methyl cis-15-tetracosenoate)
37. Cis-4,7,10,13,16,19-Docosahexaenoate

Additional Information:
Analytical Method Parameters:

Column: SP-2560, 100 m x 0.25 mm x 0.2 µm df

100°C (4 min) to 240°C at 3°C/min
Detector: FID, 250°C
Injection Volume: 1 µl

Page 4 of 5
 Certiied
Certiicate of Analysis Reference
SUPELCO 37 COMPONENT FAME MIX
Material
Product ID CRM47885
Lot LRAB8645
Expiration Date April 2021
Manufacturing Date May 2018
Storage Conditions Freeze
Solvent/Matrix METHYLENE CHLORIDE

1 Certified value - based on the gravimetric preparation (corrected by the raw material purity) and analytically verified by RTC with associated uncertainties from the preparation and analytical procedures.
2 The standard deviation is the robust statistical standard deviation from the round robin interlaboratory study.
3 Acceptance limits are based on Interlaboratory Study Results. These ranges are recommendations only.
4 Ucrm - Uncertainty values in this document are expressed as Expanded Uncertainty (Ucrm) corresponding to the 95% confidence interval. Ucrm is derived from the combined standard uncertainty multiplied by
the coverage factor k, which is obtained from a t-distribution and degrees of freedom. The components of combined standard uncertainty include the uncertainties due to characterization, homogeneity, long term
stability, and short term stability (transport). The components due to stability are generally considered to be negligible unless otherwise indicated by stability studies. The mathematical representation of the Ucrm
calculation is as follows:
2 2 2
u CRM = u char + u hom ogeneity + u stability
5 k: Coverage factor derived from a t-distribution table, based on the degrees of freedom of the data set. Confidence interval = 95%

Traceability: The standard was manufactured under an ISO/IEC 17025:2005 certified quality system. The balance used to weigh raw materials is accurate to +/- 0.0001g and calibrated
regularly using mass standards traceable to NIST. All dilutions were preformed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and
specified above.

Homogeneity: Homogeneity was assessed in accordance with ISO Guide 35. Completed units were sampled using a random stratified sampling protocol. The results of chemical
analysis were then compared using a one-way analysis of variance approach as described by TNI EL-V3-2009 Appendix A.2. See Instructions for minimum sub-sample size.

Expiration is at end of month given on certificate and label.

THIS PRODUCT WAS DESIGNED, PRODUCED AND VERIFIED FOR ACCURACY AND STABILITY IN ACCORDANCE WITH ISO/IEC 17025:2005 (ANAB Cert AT-1467) and ISO
GUIDE 34:2009 (ANAB Cert AR-1470).

MSDS reports for components comprising greater than 1.0% of the solution or 0.1% for components known to be carcinogens are available upon request.

Robert O'Brien - QC Supervisor Mark Pooler - QA Supervisor

Certiication Date October 24, 2018
Version -10242018

2931 Soldier Springs Rd. Laramie, Wyoming 82070 USA

307-742-5452
[email protected] www.sigma-aldrich.com
Page 5 of 5