College of Natural Sciences: Arbaminch University
College of Natural Sciences: Arbaminch University
DEPARTMENT OF CHEMISTRY
Submitted by
ADVISOR CO-ADVISOR
Dr. J. ETHIRAJ
February 1, 2019
CERTIFICAION
This is to certify that the proposal entitles, THE EXTRACTION OF FIBRE FROM SISAL
PLANT BY CHEMICAL PROCESS AND STUDY OF STRUCTURAL AND
COMFORTABLE CHEMICAL PROPERTIES FOR APPAREL USE.
Submitted in partial fulfillment for the Bachelor of Science (B.Sc.) Degree in Industrial
chemistry of Undergraduate program of Department of chemistry, College of Natural Science,
Arba Minch University, and is a record of the original proposal carried out by MUKTAR
KASSIM ID:RNS/192/08, GASITACHEW SEYOUM ID:RNS/226/08, FIREHIWOT
TAMIRAT ID:RNS/532/08, ZINASH GIRMA ID:RNS/541/08, ERIT SALAHADIN
ID:RNS/396/08, ABEBA ZERU ID:RNS/565/08.The assistance and the help received during
the course of this investigation have been duly acknowledge. Therefore, we recommend that it
would be accepted as fulfilling the BSc research proposal requirement.
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Table of Contents
CERTIFICAION..............................................................................................................................I
List of tables..................................................................................................................................IV
List of schemes...............................................................................................................................V
Acronyms......................................................................................................................................VI
Abstract............................................................................................................................................1
CHAPTER ONE..............................................................................................................................2
1. INTRODUCTION.......................................................................................................................2
1.1. Fibre......................................................................................................................................2
CHAPTER TWO.............................................................................................................................5
2. REVIEW OF LETREATURE.....................................................................................................5
CHAPTER THREE.........................................................................................................................9
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3. MATERIALS AND METHODS................................................................................................9
3.1. Materials................................................................................................................................9
3.2. Methods.................................................................................................................................9
CHAPTER FOUR.........................................................................................................................11
References......................................................................................................................................13
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List of tables
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List of schemes
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Acronyms
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Abstract
In view of recent global environmental issues, scientists worldwide have begun to show interest
in exploiting the full potential of natural fibers and their diverse uses. Historically fiber is
produced by using retting and mechanical process, which is not efficient in the removal of fibre
compounds. This study will be taken to investigate the chemical and physical character of the
fibre upon extraction by using chemical process. Chemical extraction of fibre have two stages,
preliminary stage and laboratory stage. Primarily attaining maturity, sisal leaves will be
harvested manually by cutting from the leaf base with a sharp specialized crescent like knife
having long handle. The harvested the fibres were thoroughly washed with distilled water and
dried in a vacuum oven pre-set at 80ºC for 24 hours after which raw fibres were sampled. They
were then treated with diethyl ether: Hexane: Ethanol (2:2:1). After the pre-treatment, fibres
were thoroughly washed with distilled water and dried in an oven at 80ºC for 24 hours and the
fibres were then treated with sodium hydroxide and enzymes. After the extraction process will be
completed, the length, diameter, moisture adsorption properties, physical shape, color, the
chemical composition of fibre after treated with NaOH and some other physico-chemical
properties of fibre will be evaluated.
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CHAPTER ONE
1. INTRODUCTION
1.1. Fibre
Fibres are long strands of molecules inter-connected to form a linear, string-like structure. They
can be natural, synthetic or sometimes semi-synthetic. Natural fibres as bio-based fibres from
vegetable and animal origin. Natural fibres are greatly elongated substances that can be spun into
filaments, thread or rope. This definition of natural fibres includes all natural cellulosic fibres,
such as cotton, jute, sisal, flax, hemp, etc. and protein based fibres such as wool and silk. The
United Nations declared 2009 to be the International year of natural fibres, with the central
objective of promoting the use of natural fibres in current and novel applications, which
contributes to increased levels of income for fibre producers, processors and traders, while at the
same time contributing to the increased use of environmentally friendly materials in those
applications [1].
Products from plants are assuming increasing importance to the economy and wellbeing of any
country. Utilization of plant resources for generating employment in rural areas is natural, cost
effective and ecologically sustainable. Since long time the mankind is associated with natural
fibres and they have been used by them for various purposes and have been incorporated in their
livelihood. Be it basketry, mats or small useful products, appropriate use of the locally available
material is always the solution. India has a vast resource of different natural fibers viz., jute,
sisal, banana, coir etc., which are abundantly available with a production capacity of 400 million
tons per year. Among others, sisal has an edge as it can be grown in wastelands; require
minimum maintenance; withstand in many agro ecological conditions and produces continuous
fiber for seven to eight years. It is usually grown on land that is unsuitable for any other
agricultural activity apart from grazing. Other advantages are that the crop is drought resistant,
does not require the use of fertilizers, herbicides or insecticides, can be inter-cropped and inter-
grazed. For instance, in hundred years of commercial sisal growing in Tanzania there has not
been a year when there was a drought to destroy sisal plants [3].
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1.1.1. Sisal fiber
Sisal is a vegetable fibre extract from leaves of an Agave (Agave sisalana Perrine), a major
tropical fibre used in agricultural and parceling twine of various kinds in addition to ropes, sacks,
carpets, and upholstery. Agave is a genus that includes the common sisal (A.sisalana) and many
other species such as Agave fourcryodes (Henequen). The Agaves are indigenous to tropical and
sub-tropical regions of Southern America, Mexico, Southern Coast of United States of America
and the Caribbean Island. It was introduced to Tanzania by a German agronomist in 1893 who
imported bulbils from Florida, USA. From there, sisal spread to Kenya and other parts of East,
Central and Southern Africa [4].
Sisal is a xerophyte, monocarp, semi-perennial leaf fibre producing plant. The plant has short
stem bears rosette of leaves that are sessile, linear lanceolate attains a length of 1m or more. The
leaves are thick, fleshy and often covered with waxy layer, typical characteristics of xerophytic
plants. A healthy sisal plant produces about 200-250 leaves during its10-12 years life span, after
which it produces a long flowering axis called ‘pole’. Different species of Agave
family) namely A. sisalana, A. cantala, A. Vera amaniensis, A. angustifolia and A. fourcryodes
hard fibre from its leaf. However, among the different species, A. sisalana contributes nearly
85% of the total sisal fibre production of the World. A. sisalana is a native of the Yucatan
area of Mexico where the fibre had been used by the native Mexican people for centuries. Later,
around 1836, sisal was introduced to Florida. Other than Mexico, wide cultivation of
sisal was started in Tanzania, where it was introduced by Dr. Richard Hindorf, an Agronomist
from Germany. In the present world, major sisal producing countries are Brazil, Kenya,
Tanzania, Madagascar, China, Mexico and Haiti (Table 1). In the modern world, sisal/ agave was
extensively studied Howard Scott Gentry (1903-1993) who was an American botanist recognized
as the world's leading authority on the agaves. Sisal fibre is very commonly used in the shipping
industry for mooring small craft, lashing, and handling cargo. Besides, it has several other
domestic to industrial uses including high strength requiring long -lasting geo-textile and
specialty composites. Sisal is also an excellent CDM (Clean Development Mechanism) crop for
bioethanol as well as for afforestation over poor quality arid lands giving both permanent carbon
credits of forestry for carbon sequestration. In general sisal is not much infested by many
disease and insect pest; and therefore, sisal plantation does not produce pesticide load to the
environment. Besides, sisal plants reduce soil erosion through its extensive root system and
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contributes positively to watershed management. Sisal has several distinguishing characteristics
which makes sisal a ‘specialty crop’ for conservation agriculture [2].
Mechanical fiber extraction is small scale operations by using machines and are not efficient in
the removal of cementing compounds (mostly waxes, hemicelluloses, lignin and hydrocarbons)
between fibers.
Hence this study will be taken to investigate the extraction of fiber by using chemicals and no
specify study on the extraction of the sisal fiber by using the chemical process. As a pre-
treatment step, distilled water is used to clean raw sisal plant leaves. Then after drying the fiber
is treated with diethyl ether: Hexane: Ethanol (2:2:1) followed by ethanol, to ensure the removal
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of all polar and nonpolar compounds which includes waxes and other extractives such that only
macro molecules or polymers remain. Then alkali and enzymatic treatments are followed to
improve quality of the fiber. Alkali used in fiber extraction dissolves the lignocellulose material
between fibers and separates structural linkages between lignin and cellulose, which leads to
increased surface area as well as a degree of polymerization and lowers the breaking strength of
fibers. In enzymatic process of fiber extraction, there is degradation of lignocellulose component
in fibers by enzymes resulting increased fiber swelling and lowers the degree of polymerization.
Fibers extracted by using enzymes are more pliable and softer.
The capacity of Denilite II S (pH 7), Scourzyme L (pH 9) and Cellusoft Conc. L (pH 7)
enzymes and alkaline NaOH to remove the gluing materials found in the fibres.
To investigate chemical composition of sisal fiber after treated with sodium hydroxide,
enzymes and untreated fiber.
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treated with NaOH (8%). After extraction the chemical and physical composition of the treated
and untreated fiber is tasted.
CHAPTER TWO
2. REVIEW OF LETREATURE
Natural fibers have gained renewed interest due to their varied uses in textiles, composites,
handicrafts, packaging, etc., and consumer demand for environmentally sustainable and traceable
processing chains [8].
2.1. Classification of natural fibre
Fibers are a class of hair-like material that are continuous filaments or are in discrete elongated
pieces, similar to pieces of thread. They can be spun into filaments, thread, or rope. They can be
used as a component of composites materials. They can also be matted into sheets to make
products such as paper or felt. Fibers are of two types: natural fiber and man-made or synthetic
fiber [7].
Silk
Wood Stem/Blast Leaf Seed/Fruit Grass wool
Scheme.1.
f Classification of fibre
Fique
2.2. Sources of natural fibres
Natural fibers include those made from plant, animal and mineral sources. Natural fibers can be
classified according to their origin [7].
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2.2.1. Vegetable fibers
Vegetable fibers are generally comprised mainly of cellulose: examples include cotton, jute, flax,
ramie, sisal, and hemp. Cellulose fibers serve in the manufacture of paper and cloth. This fiber
can be further categorized into the following:
Seed fiber: Fibers collected from seeds or seed cases. E.g. cotton and kapok.
Leaf fiber: Fibers collected from leaves. E.g. sisal and agave.
Bast fiber or skin fiber: Fibers are collected from the skin or bast surrounding the stem of
their respective plant. These fibers have higher tensile strength than other fibers.
Therefore, these fibers are used for durable yarn, fabric, packaging, and paper. Some
examples are flax, jute, kenaf, industrial hemp, ramie, rattan, soybean fiber, and even
vine fibers and banana fibers.
Fruit fiber: Fibers are collected from the fruit of the plant, e.g. coconut (coir) fiber.
Stalk fiber: Fibers are actually the stalks of the plant. E.g. straws of wheat, rice, barley,
and other crops including bamboo and grass. Tree wood is also such a fiber.
Stalk fiber: Fibers are actually the stalks of the plant. E.g. straws of wheat, rice, barley,
and other crops including bamboo and grass. Tree wood is also such a fiber.
The most used natural fibers are cotton, flax and hemp, although sisal, jute, kenaf, and coconut
are also widely used. Hemp fibers are mainly used for ropes and aerofoils because of their high
suppleness and resistance within an aggressive environment. Hemp fibers are, for example,
currently used as a seal within the heating and sanitary industries [7].
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Brazil, as well as to countries in Africa, notably Tanzania and Kenya, and Asia. Among flax,
hemp, abaca, sun hemp and other agro-based fiber species, annual sisal production is the second
largest worldwide, after cotton.
Sisal does not build up static nor does it trap dust, so vacuuming is the only maintenance
required. High spill areas should be treated with a fiber sealer and for spot removal, a dry
cleaning powder is recommended. Depending on climatic conditions, sisal will absorb air
humidity or release it causing expansion or contraction. Sisal is not recommended for areas that
receive wet spills, or rain or snow [7].
Agave sisalana is the botanical name for sisal plant. It originated from Mexico and availability is
found more in Brazil, Tanzania, Kenya and some regions of Asia. Sisal fibers are produced from
sisal leaves. These plants live for 7-10 years producing approximately 200-250 leaves. These
fibers are stiff in nature and it is used for producing the following items
Rope, twines and carpets
Marine applications
Textile industries
Wall coverings and dartboards [5].
Due to superior mechanical properties recyclable nature sisal fibre can be used as potential input
material for making composites for application in buildings, automobiles, railways, geo-textiles,
packing industries etc. (sheme.2)
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decomposition of sisal leaves which separates fibre from the pith. This process takes 15-21 days
for a single cycle of extraction and degrades the quality of fibre. Mechanical extraction with the
help of decortication (Raspador) machine is best suited for small scale operations at village level.
It is a semi-automatic machine running either on diesel or electricity and can extract fibre from
12 sisal leaves per minute.
2.3.1. Fiber Properties
The important chemical components of the sisal fibre are shown in Table 1. Based on the
composition, it can be inferred that the sisal fibre is harder (greater rigidity and lower flexibility)
and coarser than other bast and leaf fibres because of the high lignin and pectin content. Apart
from this, the superior engineering properties (diameter 50–200 m; microfibril angle 10–220,
Ultimate Tensile Strength of 468–640 Mpa; Modulus of 9.40–15.80 Gpa and elongation of 3–
7%) makes it an excellent material for manufacturing high strength textile and reinforcement in
composites for various applications [3].
Table 1: Chemical composition of sisal fiber
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CHAPTER THREE
3.1. Materials
Diethyl ether, hexane, ethanol, NaOH etc., Sisal leaves of long length will be collected from the
field.
3.2. Methods
3.2.1. Preliminary stage
Attaining maturity, sisal leaves will be harvested manually by cutting from the leaf base with a
sharp specialized crescent like knife having long handle [2].
After the pre-treatment, fibres were thoroughly washed with distilled water and dried in an oven
at 80ºC for 24 hours. The fibres were then treated with sodium hydroxide and enzymes. The
enzymatic treatment was done in a step-wise format, because the three enzymes used differed in
their pH for optimum function. The enzymes used were: Denilite II S (pH 7), Scourzyme L (pH
9) and Cellusoft Conc. L (pH 7) sourced from a ligase, pectinase and hemicelluloses,
respectively. After the treatment, the fibres were then tested for both physical chemical
properties for comparison purposes. Hence, proper drying is important the fibers were dried
under a shade to avoid bleaching by direct sunlight [1].
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3.3. Evaluation of fibre
Fiber length, fiber diameter, Moisture Absorption Properties, visual and hand evaluation such as;
color, texture, luster, smoothness, flexibility, physical shape and the burning characteristics of
the fiber like other known cellulosic fibers will be tested [6].
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CHAPTER FOUR
Time
1 Topic X
selection
2 Searching x x
literature
3 Proposal x x x
development
4 Advisor x x x x x x
contact
5 Report x
writing
6 Presentation x
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Table 3.Schedule for budget plan
Pen No 2 5 00 10 00
Pencil No 1 3 50 3 50
Ruler No 1 5 00 5 00
Note pad No 1 25 00 25 00
Samples No 5 15 00 75 00
Print Pages 90 60 54 00
Total 582 50
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References
1. Vilane, V.S.1, Thwala, J.M.1, Ndlovu, T.1, Zwane, P.E.2 & Masarirambi, M.T.3 “The bio-
processing of fibres from Agave sisalana and Agave americana” Research Application Summary
PP: 393 – 399
2. Sitangshu Sarkar and Jha, A. K. (2017) “research for sisal (agave sp.) Fibre production in
india.” International Journal of Current Research Vol. 9 (11), PP 61136-61141,
3. P.Srinivasakumar, M.J.Nandan, Dr.C.Udaya Kiran, Dr.K.Prahlada Rao (2013) “Sisal and its
Potential for Creating Innovative Employment Opportunities and Economic Prospects.” Journal
of Mechanical and Civil Engineering (IOSR-JMCE), Vol. 8(6), PP 01-08.
4. PKF Consulting Ltd (2005) “Kenyan sisal industry overview” International Research
Network, PP 1-3
5. Anna University Chennai “extraction of fibre from banana and sisal plant.” 600 [025]
6. Ashish Hulle, Pradyumkumar Kadole and Pooja Katkar (2015)“Agava Americans leafs fiber.”
fibers, vol. 3, PP 66-75.
7. D. Chandramohan & .K. Marimuthu (2011) “A review on natural fibers” journal of
mechanical Engineering, vol. 8(2), PP 196-198
8. Laura Bacci, Sara Di Lonardo, Lorenzo Albanese, Giorgio Mastromei and Brunella Perito
(2010) “Effect of different extraction methods on fiber quality of nettle.” Textile research
Journal, vol. 81(8), PP 27-29.
9. R.V. Subbarao and V.Vasudeva Rao “Additional employment and income opportunities
through sisal fiber in the tribal and forest areas of Andhra Pradesh.” Forum for integrated
development. PP 212-215.
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