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RANDOX Procedure For Glucose GOD-PAP Assay Without Deproteinisation

This document provides instructions for performing a glucose assay using the glucose oxidase method without deproteinization. The assay is to be run on samples and standards using a spectrophotometer at 500nm or 546nm. Results are to be calculated based on absorbance readings of samples compared to a standard curve. Quality control samples within specified ranges are recommended to be run daily to ensure accuracy of results.

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Yeliztli Marin Celso
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790 views1 page

RANDOX Procedure For Glucose GOD-PAP Assay Without Deproteinisation

This document provides instructions for performing a glucose assay using the glucose oxidase method without deproteinization. The assay is to be run on samples and standards using a spectrophotometer at 500nm or 546nm. Results are to be calculated based on absorbance readings of samples compared to a standard curve. Quality control samples within specified ranges are recommended to be run daily to ensure accuracy of results.

Uploaded by

Yeliztli Marin Celso
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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RANDOX MANUAL GL 2623

CE
PROCEDURE FOR GLUCOSE GOD-PAP ASSAY NORMAL VALUES0,4)
wITHOUT DEPROTEINISATION
Serum/Plasma 4.2-6.4 mmol/l (75-115 mg/di)
Wavelength: 500 nm, Hg 546 nm
Cuvette: cm path length Random urine 0. 0.8 mmol/l ( I5 mg/dl)
Temperature: I5-25°C or 37°C
Measurement against reagent blank 24 hour urine <2.78 mmol/d (0.5 g/d)

Pipette into test tubes It is recommended that each laboratory ce_tablish its own
reference range to reflect the age, sex, diet and geographical
Macro Semi Micro location of the population.

Standard Reagent Standard Reagent LINEARITY


or Sample Blank or Sample Blank The test is linear up to a glucose concentration of
22.2 mmoll (400 mg/dl). Samples above this concentration
Standard or sample 20 l 10 ul should be diluted 1+2 with distilled water and the result
Reagent 2000 2000 u 1000 ul 1000 l multiplied by 3.

SENSITIVITY
Mix, incubate for 25 min at 15-25°C or 10 min at 37°C. Measure lt is recommended that each laboratory establishes its own range
the absorbance of the standard (A sandard) and the sample (A of sensitivity as this is limited by the sensicivicy of the
sample) against the reagent blank within spectrophotometer used. Under the conditions of the assay a
60 minutes. Although the absorbance may be read at any time change of 0.01 absorbance units is equivalent to
up to 60 minutes after the specified incubation time, the time 0.013 mmol/l (0.23 mg/dl).
interval from sample addition to read time must be exactly the
same for Standard/Control and Sample. REFERENCES
I.Barham, D, and Trinder, P. Analyst 1972; 97: I42.
CALCULATION 2. Clinical Chemistry Principles and Techniques, Second Edition,
RJ. Henry. D.C. Cannon and J.W. Winkelman Editors, Harper
and Row, Maryland, USA, I288, I974.
Asample Standard conc.
Glucose 3. Teuscher, A. and Richterich, P. Schweiz Med. Wschr., 1971;
(mmol/l) (mmol/l)
Concentration Asandard 101:345 and 390.
4. Tietz. N.W. Clinical Guide to laboratory tests. 2nd edition.
Philadelphia. Pa: WB Saunders Co.; I990: 246-250
Asample
x Standard conc.
(mg/dl)
Astandard (mg/d)
QUALITY CONTROL
Randox Assayed Multi-sera, Level 2 and Level 3 are recommended
for daily quality control. Two levels of controls should be assayed
at least once a day. Values obtained should fall within a specified
range. If these values fall outside the range and repetition excludes
error, the following steps should be taken:
. Check instrument settings and light source
2. Check cleanliness of all equipment in use.
3. Check water, contaminants i.e. bacterial growth may contribute
to inaccurate results.
4. Check reaction temperature.
5. Check expiry date of kit and contents.
6. Contact Randox Laboratories Customier Technical
Support, Northern Ireland (028) 94422413.

INTERFERENCES
The analytes below were tested up to the following levels and
were found not to interfere:

Haemoglobin 000 mg/d


Free Bilirubin '
25 mg/'dl
Conjugate Bilirubin 25 mg/dl
Triglycerides 1000 mg/d
Intralipid 400 mg/dl

Revised 13 Nov 08, bm


Rev. 00

Randox Laboratories Limited, 55 Diamond Road, Crumlin, County Antrim, BT29 4QY, United Kingdom PAGE 2 OF 2
T +44 (0) 28 9442
2413
F +44 (0) 28 9445 2912 E
[email protected]
Iwww.randox.com

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