BIOL 222 Lab
BIOL 222 Lab
Group Team
Results
The highest weight change percentage seen in the apple in Table 1 was 3.5% at 0.55 (M) sucrose
solution concentration. Figure 1 shows that as sucrose concentration increased in the solution,
the weight gain percentage decreased. Higher water potential in the sucrose solution helped the
apples to gain water. Figure 1 also shows that impossible to reach the isotonic point using
provided solute concentration. The weight change percentage in Figure 1 doesn’t seem becomes
zero, this is because of the water potential differential in apple solute concentration and sucrose
solution concentration. At zero weight change percentage apple tissue will be isotonic, which is
The weight of the potatoes was lost more in the higher sucrose concentration solution and as the
concentration of the sucrose solution decreased weight change percentage also decreased as
shown in Table 2. Potatoes had higher water potential as compared to the sucrose solution, that’s
why potatoes lost weight. Figure 2 shows that at 0.29 (M) of sucrose solution, there is no weight
change in potatoes, and potato tissue at this point was isotonic. The solute concentration of
potatoes and solute concentration of sucrose solution is the same at the isotonic state which is
0.29 (M).
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Discussion
Several factors affect the rate of osmosis such as the size of chunks of potatoes or apples and
temperature and pH, however in lab investigations done on the concentration of sugar solution
(Lenart and J.M., 1984). Apple and Potato pieces used in the experiment were the dependent
Apples gained weight because apple was a higher solute concentration as compared to sucrose
solute. Figure 1 shows that the sucrose solution concentration increased from 0.50 to 0.75 (M)
the weight change percentage decreased. The Scattered graph shows the correlation between the
sucrose solution concentration and the weight change percentage of the apples. The correlation is
negative as one variable increases; the other variable also decreased. Isotonic point is not
achieved in the experiment. The factor responsible for not achieving isotonic point is variables
such as temperature, pH, atmospheric pressure, or limitation of the experiment such as time.
Potatoes lost weight because the solute concentration of the potatoes was lower as compared to
the sucrose solution. Figure 2 shows that the sucrose solution concentration at 0.50 (M) reduced
the mass around by 10%. Potatoes were placed in sucrose solutions (0.3,0.35,0.40 and 0.45 M)
and lost mass due to osmosis, transferring water from the potatoes to the sucrose solution. The
Scattered graph shows the correlation between the sucrose solution concentration and the weight
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change percentage of the potatoes. The correlation is positive as one variable increased; the other
References
Lenart, A., and J. M. Flink. "Osmotic Concentration of Potato." International Journal of Food
Science & Technology 19.1 (1984): 65-89. Web.
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Annexure
Table and Figures
Sucrose concentration (M) % Change in weight
0.55 3.50
0.6 3.13
0.65 2.46
0.7 2.27
Table 1 Influence of sucrose concentration (M) on Apple Mass
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2.46
2.5 2.27
2
1.5
1
0.5
0
0.5 0.55 0.6 0.65 0.7 0.75
Sucrose Concenration (M)
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Sucrose concentration (M) % Change in weight
0.3 -0.8522
0.35 -2.0057
0.4 -6.4139
0.45 -9.444
Table 2 Influence of sucrose concentration (M) on Potato Mass
-2.00
% Change of weight
-3.00
-4.00
-5.00
-6.00
-7.00
-8.00
-9.00
-10.00
Sucrose Concentration (M)