LIST OF ITEMS, TECHNICAL SPECIFICATIONS AND QUANTITY

FOR ONE LABORATORY FOR CULTURE & SENSITIVITY FOR RNTCP

Sl. No. Item Technical Specifications Quantity Remarks
per
Consignee
Schedule I
1 Biological Type: BIOSAFETY CABINET, CLASS II BI (As per NSF 2 Equipment quoted should
Safety guidelines) comply with Indian
Cabinets The basic equipment shall consist of exhaust HEPA filter, Standards Institutions
‘Supply HEPA filter, HEPA filter for supply air, negative Guidelines or any other
pressure exhaust plenum, front opening sash with either National or International
counter weight of motorized movement, suitable blower Guidelines.
assembly, necessary lighting, indicators and controls for the
cabinet. The equipment should be mounted on a stand with
leveling feet. The exhaust plenum should be under negative
pressure, hard ducted to the outside.
Use of the Equipment: - Transfer of microorganisms to media
containers.
HEPA FILTER: Face dimensions: 4ft. (L) X 2ft. (W) X 6ft .
The HEPA filter should have rated efficiency of 99.97% (or
better) at 0.3 microns to provide product protection of Class
100 or exceeding Class 100 requirements of Federal Standards
209E or equivalent ISO within the work area.
Face Velocity: At least 100 fpm
Light Intensity at work surface: 800 lux or more over the
entire work surface.
Noise level : <70 dBA.
UV germicidal lamp intensity: >40 microwatt/sq.cm over the
entire work surface.
Construction : Main body, side and rear panel: Electro-
galvanized Steel or Mild Steel, oven baked epoxy powder
coated finish. Workable (surface): SS304 or SS 316.
Switches and indicators: Individual switches and indicator
lamps for blower motor, florescent lamp and UV lamp.
Differential pressure gauge : Scale display in cms of water
Electrical protection : The LAF should be fitted with earth
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leakage circuit breaker (ELCB)
Other fittings required for Attaching auxiliary services :
Electrical outlet socket (5 ampere rating) qty: 2 nos.
Prefilter :- one

The Biosafety Cabinet should be tested and comply with the Note:- All the tests will
following requirements (at site):- have to be conducted by
1. Down flow velocity and Volume Test. an Accredited Agency
2. Inflow Velocity Test. which shall then issue
3. Airflow Smoke Pattern Test. Test Certificate and send
4. HEPA Filter Leakage Test. it to the respective
5. Cabinet Leakage Test. consignees.
6. Electrical Leakage : Ground Circuit Resistance and Subsequently, the tests
. Polarity Test shall be conducted (after
7. Lighting Intensity Test. installation at the
8. Vibration Test. respective consignee’s
9. Noise Level Test. Laboratory site) by the
10. UV Lamp Intensity Test. supplier and ‘Certificate
11. Alarms and indicators test (if provided). of Satisfactory
12. The differential pressure gauge should be calibrated. Installation’ shall be
Spare accessories : HEPA Filter (one)-dimensions same as obtained from the
above, Prefilters (two). respective consignees.

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Sl. No Item Technical Specifications Quantity per Remarks
Consignee
Schedule II
1 Universal 28ml, wide neck, with aluminium screw cap and liners pack of 10,000 “Fisher” make
Containers 20 bottles. Weight of each bottle in gms (without screw cap or equivalent.
(McCartney Bottles) and Neoprene washer)= 50+1, The Bidder
Length of bottle in mm = 82 must specify
Maximum external circumference of bottle in mm = 92 the make of
Type of bottle (thickness and surface: bottom and side of each the Containers
bottle) = Even thickness without air bubbles in the glass, clear it intends to
glass supply.
Diameter of the bottom of bottle in mm to fit within centrifuge
bucket of inner diameter of 30 mm = 28.2
Capacity of each bottle in ml (volume) = 28
Bottle should be autoclavable, should not become opaque on
autoclaving.
Screw cap for the bottle: Weight in Gms without washer =
1.6+0.1 gms
screw cap: made of aluminum to fit tightly all bottles (leak
proof)
screw cap: Inner diameter of screw cap without Neoprene
washer in mm = 2.7
Neoprene (soft) washers thickness in mm = 2.5
Neoprene washer diameter in mm = 2.7
Neoprene washers should withstand repeated autoclave.
2 Spare Caps for Spare caps with liners for Universal Containers 28ML, wide 10 packs (100
Universal neck, pack of 100 Nos. each)
Containers

3
Sl. No Item Technical Specifications Quantity per Remarks
Consignee
Schedule III
1 Autoclave With water level indicator, pressure gauge, steam 1 Applications:-
(Vertical) release cock, spring loaded safety valve, Triple 1) For effective
walled construction, all the three walls are made of sterilization for
thick high grade stainless steel sheet of SS-304 grade. smaller work load.
The lid made of stainless steel sheet, Unit fitted with 2) For
Double safety radial locking system with paddle decontamination of
lifting device, made of MS chrome plated, with built- infected material
in safety valve, pressure gauge, pressure release valve prior to its disposal.
and water level indicator. Pressure adjustable from 5 3) For faster work in
psi to 20 psi with an accuracy of +/- 1 psi, with the laboratory.
automatic pressure control switch. Supply complete
with stainless steel basket, chord and plug. The apparatus should
Working Chamber: Height x Internal confirm to national or
Diameter:500mm x 300mm Electrically heated by international standards
immersion type heaters bearing ISI mark, to work on with latest amendments
220 volts single phase 50 cycles. The pressure inside covering
the chamber is variable from 5 psi. to 20 psi.. Markings, Safety
Working Temperature : 121° C, Hydrostatic Pressure requirements with
: 2.5 kg/cm sq. ( 35 psi ), stainless steel steam release recommendations of safe
valve, spring loaded safety valve of stainless steel operations from any
(grade SS-304), water inlet and water valves, water reputed firm with ISO
level indicating gauge glass with stainless steel 9001:2000 & CE
guard, pressure gauge, electrical control box, fitted Certification. Voltage
with toggle switch, indicating Neon lamps and steam regulator of appropriate
release valve, foot paddle lifting device. rating to be included to
cope with 160-260 V.
Automatic Pressure Control Switch - To cut-off the
current from the heating elements, when the desired/
set pressure value level is attained inside the chamber
and restarts the mechanism once the pressure inside
the chamber falls from the desired level.
Automatic Water Cut-off Device – To protect the
heaters from running dry and to ensure that the
machine is automatically switched off in case the

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 desired water level falls below the prescribed level.
Temperature Indicator to indicate the temperature
inside the chamber.
Timer with Alarm System - To regulate the
sterilization time of the media to be sterilized with a
buzzer
2 Fluorescent Achromatic Objectives : 4x/0.1, 10x/0.25 & 1 The apparatus should
Microscope 100x/1.25 Oil (Spring), Fluorescence objectives : FL confirm to IS:4381-1967
10x and 40x both/0.65 (Spring), 'Wide Field (reaffirmed 2007) with
Eyepieces : 10x Paired, Nosepiece : Quadruple, latest amendments or
Reflected Fluorescence Unit Power unit 110 V/ 220 equivalent national or
V, Mercury lamp house 100 W/DC, Reflected international standards for
fluorescence illuminator (trinocular, B.G. excition general requirements for
system) including installation. optical components and
optical instruments,
marking and packing.
Voltage regulator of
appropriate rating to be
included to cope with 160-
260 V.

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3 Electronic General purpose table top laboratory balance, 1 Equipment quoted should
Balance Stainless Steel, 220-230 V, stainless steel platform, comply with Indian
keypad auto calibration function, auto off, battery life Standards Institutions
of at least three months after installation, overload Guidelines or any other
and under load, low battery LCD/LED indicator. National or International
Range: Wide range: 0.001 – 120 gms, (three digit Guidelines. Voltage
decimal), Resolution: 0.001 gms, Accuracy 0.001 regulator of appropriate
gm, Repeatability (SD): 0.01 gm., pan diameter 80 rating to be included to cope
mm., weighing pan enclosed in glass shield ,with full with 160 – 260 V
range tare, AC adapter.
4 Shaker For culture processing, Maximum load: 15kg; 1 Equipment quoted should
stainless steel and anodized aluminium parts; baked comply with Indian
polyester finish; digital speed control; 20-240 rpm, Standards Institutions
adjustable strokes, for use at 240volts AC, 50HZ Guidelines or any other
Dimensions: 120cms (L)x 60cms (W)x 20cms (H), National or International
accommodates containers of any size. Guidelines. Voltage
regulator of appropriate
rating to be included to cope
with 160-260 V.

5 Centrifuge Table Top, AC, 50HZ, with swing out rotor, adapters 1 Equipment quoted should
4 Nos , to hold universal containers; and hermetically comply with Indian
sealed caps 4 Nos, with windshield, and ‘o’ ring for Standards Institutions
full aerosol containment, safety interlocked lid, bio- Guidelines or any other
safety features incorporated. National or International
Max. Speed: 6,000rpm Guidelines. Voltage
Max. RCF: 6,240 x g regulator of appropriate
Max. Capacity: 4 x 400 ml rating to be included to cope
Run Time: 1– 99 mins, Continuous operation with 160-260 V.
Voltage: 230 V, 50 Hz,
Programme Memory: Values last entered remain in Note:- Centrifuge should be
memory. Data saved if power is interrupted, 1.0 non- hermetically sealed, so as to
refrigerated, maximum capacity 4 x 400 ml to hold prevent release of aerosols
McCartney bottles (universal containers). into the air thus preventing
Other Technical details health hazards.
SWING-OUT ROTOR: 1 No.
Max. Speed: 4,000rpm Clarification:-

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 Max. RCF: 3,345 x g The first Max speed and Max
Max. Capacity: 4 x 400 ml RCF are for the main
Round Bucket 400ml for adapter inclusive of Centrifuge and the second
Polypropylene bottle 4 Nos. Sealing cap for round Max speed and Max RCF are
bucket: 4 Nos for SWING-OUT ROTOR.
Each 400ml adapter should hold at least 4
McCartney bottles (universal containers) of size
length 82 mm, diameter of the bottom 28.2 mm,
volume 28 ml.
Adaptor: 4 x50 ml: 4 Nos

6 Inspissator A shallow polish stainless tray rested inside a tank 2 Voltage regulators of
containing water. The whole undersurface of the tray appropriate rating to be
is in contact with water at a constant temperature included for each item to
which ensures that the temperature of the McCartney cope with 160-260 V.
bottles with media is also constant. The surface of the
tray is a series of sloping steps (at 9 degree angle
above the horizontal) and will hold 162 universal
containers. A blanket is placed over the containers to
exclude draughts and a quilted cover provides
thermal insulation: both blanket and quilt are made
from insect-resistant materials. The temperature of
the water under the tray is controlled by a digital
immersion thermostat. Accuracy and reproducibility
of set temperature are ensured with the digital display
of actual and, at the touch of a button, set
temperature. The control unit is mounted on a bridge
plate over one end of the bath, from which heater,
stirrer and temperature sensors project down into the
bath. All moving parts are incorporated in the
control unit which removable for servicing. The tray
and tank are made of polished stainless steel and are
fitted in an outer case of laminated wood. A constant
level device is fitted is fitted to maintain the water
level despite evaporation losses. Specifications: Std
temperature: 85c;
Operating temp. range: ambient +5 to 90C.;
temperature display: LED; Display resolution: 0.1c;
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 Uniformity: tray surface + or - 0.7c;Heater power:
(approx.)1.4Kw, 230V; Tank capacity: (approx.)45
lit; Heat up rate 20 to 85 c; 3.5 hours; working area:
1eng/width: (approx.) 820/594mm; overall
dimensions (approx).: 1/w/h: 1040/600/380mm; Over
temperature protection: Fixed cut-out; Electrical
power: 220-240V 50/60 Hz, 1.5kW(approx.) ,
Approx.weight: 25-35 kgs.
7 Bottle Washing Electrically operated, with a quick release chuck 1 Equipment quoted should
Machine taking a wide variety of interchangeable nylon comply with Indian
brushes for cleaning syringes, test tube, McCartney Standards Institutions
bottles, and round bottles up to 250ml. Mounted on a Guidelines or any other
cast aluminium base with a perspex anti-splash National or International
shield, a white enamelled tray and incorporating a Guidelines.Voltage regulator
1/16hp motor for 250V/50Hz single phase supplies. of appropriate rating to be
Dimensions: (approx.) 62.5x27cmsx25cms.; included to cope with 160-
Approx.wt.:50-60 kgs., Accessories: Brushes, nylon, 260 V.
for round bottles capacity 30,120,250ml; brushes,
nylon, for McCartney bottles- diameter 25mm.
8 Autoclave Stainless Steel of SS-304 grade, Triple walled with a 1 Applications:-
(Horizontal) steel jacket, separate boiler, inner and outer chamber, 1) For sterilizing non
jacket and boiler made of stainless steel, The pressure infective material and
is adjustable from 5 psi to 20 psi with automatic aqueous media in
pressure control switch, which controls the pressure loosely stoppered
with an accuracy of +/- 1 psi., LW cut off, auto safety flask.
door lock, mounted on rigid tabular stand. 2) For drying of racks
Spring loaded safety valve of stainless steel. Inner and porous loads by
dimensions of chamber: (approx.) cylindrical 600mm vaccum and intake of
diameter x 1100mm deep, 9KW (approx.) electrically filtered air.
heated, Working pressure : 1.2 -1.5kg/sq. cm. 3) For larger workloads
in the laboratory.
There should be provision for vaccum for drying of
racks and porous loads. Equipment quoted should
comply with Indian
Automatic Pressure Control Switch- To cut-off the
Standards Institutions
current from the heating elements, when the desired/
Guidelines or any other
set pressure value level is attained inside the chamber
National or International
and restarts the mechanism once the pressure inside
Guidelines.Voltage regulator
the chamber falls from the desired level.
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 of appropriate rating to be
Automatic Water Cut-off Device – To protect the
included to cope with 160-
heaters from running dry and to ensure that the
260 V.
machine is automatically switched off in case the
desired water level falls below the prescribed level.
Temperature Indicator to indicate the temperature
inside the chamber.
Timer with Alarm System - To regulate the
sterilization time of the media to be sterilized and
when the desired time is passed, with a buzzer, to that
the completion of sterilization cycle.

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9 Hot Air Oven Thermostatically controlled, temperature range 1 The apparatus should confirm
ambient to 2500c with fine and coarse adjustment, to IS6365-1971(Reaffirmed
Memmert type, with fan, digital display, approx. 2006)with latest amendments
overall size 81 cm (H) x 58 cm (L) x 71 cm (W), in Indian Standard
internal size 46 cm (H) x 40 cm (W) x 35 cm (D) Specification for Laboratory
stainless steel (SS) interiors with supports on three Electric Ovens or equivalent
sides for adjustable shelves of size 38 cm x 34 cm, National or International
number of shelves: 3, Standards covering Markings,
Fan convection to ensure uniform temperature, fitted tests and Safety requirements
with load indicator and safety thermostat take over Voltage Regulator of
indicator lamp. Temperature variation +1oC, appropriate rating to be
LCD/LED indicator. included to cope with 160-
260 V.
10 Distilled Water Complete unit made in stainless steel., Constant 1 Equipment quoted should
Plant level arrangement for inlet water, Wall hanging comply with Indian Standards
model, provided with MS Bracket for wall Institutions Guidelines or any
mounting, Boiling Chamber, Lid, Cooling other National or International
Condenser and any other part coming into contact Guidelines. Voltage regulator
with water / steam should be made of 304 quality of appropriate rating to be
stainless steel. 'Heating is achieved with kettle type included to cope with 160-
immersion heaters (two)’., should work on 220/440 260 V.
volts, AC Mains, capacity of the plant should be
5 litres (approx) per hour, should be provided with
necessary safety features such as automatic
disconnection of heater connections should the still
run dry.
11 Analytical Weighing Capacity range: 1gm.- 1 kg, Readability 1 Equipment quoted should
Balance 0.1gm, Pan size 6” (approx.) dual pans of Stainless comply with Indian Standards
Steel, mechanical, table top. Standard weights Institutions Guidelines or any
should be supplied along with the Analytical balance other National or International
Guidelines.
12 Water Bath Stainless Steel, insulated double walled, inner wall 1 Equipment quoted should
of stainless steel, thermostatic temp. control from comply with Indian Standards
ambient to 85 - 900 C (Δ0.50) complete with Institutions Guidelines or any
immersion heater, Aluminium/SS cover, brass drain other National or International
cock, 220-240 volts AC, 50Hz., Dimensions Guidelines. Voltage regulator
outside:- (approx.)36x41x25 cms; inside:- of appropriate rating to be

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 (approx.)27x30x15 cms; Capacity not given because included to cope with 160-
the dimensions specified are required for the proper 260 V
functioning of the water bath in the Laboratory for
optimum utilization of the space. Power :
(approx.)480W; Digital microprocessor display to
set temp. point preventing thermal runaway.,
seamless reservoir with no welds to leak or rust, see-
through cover is hinged and removable , and steeply
gabled to accept taller samples. IEC-1010 approved.
13 Deep Freezer Upright Model: CFC free high efficiency double 1 The apparatus should confirm
refrigeration system for cooling and freezing filled in to Indian Standard Institution
the bottom. Guidelines with latest
Temperature: 0oC-86oC + - 0.5oC amendments in Indian
Temperature Control: Standard Specification for
(i) Digital temperature controller Laboratory Deep Freezers or
(ii) Microprocessor Control/Microcontroller-for equivalent National or
temperature setting International Standards
(iii) Alarms for-Voltage, Over heat, Over cool covering Markings, tests and
Size: 300 litres with minimum 3 Stainless Steel, rust Safety requirements.
free shelves. Fixed in casters for easy Voltage regulator of
maneuverability. appropriate rating to be
Electricity: 230 volts AC, 50Hz single phase. included to cope with 160-
Refrigeration system: 260 V.
Heavy-duty maintenance free refrigeration system
with hermetically sealed refrigeration compressors
and reliable cascaded refrigeration to minimize noise
and vibration. Air-cooled with security lock to
prevent unintentional switch off. Short cooling time
of 4 to 5 hours at 400C ambient temperature. The
equipment should be of continuous duty and frost-
free. Access port for CO2 back up.
Audiovisual Electronic Alarm System independent
of power supply
Electrically heated doors for quick opening of frozen
doors

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14 Pipetting Device pipette pump; automatic; to be used with glass or 2 Equipment quoted should
plastic pipettes ranging in size from 1-100ml ; comply with Indian Standards
rechargeable battery powered pipette pump Institutions Guidelines or any
incorporating a dual hydrophilic hydrophobic 0.8um other National or International
filter to prevent cross contamination; runs for 2 hrs; Guidelines.
240 volts AC, 50HZ.
15 Filter Holders— A membrane filter holder. 2 Equipment quoted should
1. Swinney Filter Two capacity holder (1) Swinney Filter holder comply with Indian Standards
Holder Inlet/Outlet Housing and Support Screen: Celcon Institutions Guidelines or any
2. Syringe Filter (acetal copolymer) Seal Washer: PTFE; Effective other National or International
Holder Filtration Area 0.8 sq.cm, Dimensions Overall Guidelines.
Length: 3.5 cm (1.4 in.)Diameter: 1.6 cm (0.6 in.)
Filter Size: Accepts 13 mm filter; Inlet/Outlet
Connections: Female threaded luer inlet, male slip
luer outlet; Maximum Operating Pressure: 2.8 bar
(280 kPa, 40 psi); autoclavable at 121 - 123 °C (250 -
253 °F) at 1.0 bar (100 kPa, 15 psi) for 15 min ; (2)
higher capacity Syringe filter: 25mm Overall Length:
2.7 cm (1.1 in.) Diameter: 3.5 cm (1.4 in.) Accepts 25
mm filter and 20 mm prefilter; Body: Delrin (acetal
resin) O-ring: Effective Filtration Area 3.7 cm2;
autoclavable at 121 - 123 °C (250 - 253 °F) at 1.0 bar
(100 kPa, 15 psi) for 15 - 20 min.Essential
Accessories : 0.22um PVDF low protein binding
membranes 13mm filter (pack of 100) & 25mm filter
discs (pack of 100).
16 Vortex Mixer Adjustable speed: 100 to 3,000 rpm, continuous and 1 Equipment quoted should
intermittent “touch-control” modes, 220-230 Volts, comply with Indian Standards
AC, 50HZ; Cup heads size: 25 mm dia. x 22 mm Institutions Guidelines or any
deep for mixing contents in McCartney bottles., other National or International
Heavy cast-metal base and suction cup to assure Guidelines. Voltage regulator
stability, prevent “walking”. of appropriate rating to be
included to cope with 160-
260 V
17 pH meter pH range 0-14 with digital display and stand by and 1 Voltage regulator of
(digital) calibration mode; hand held , pocket-size with appropriate rating to be
shielded electrode bulb and waterproof housing. 4x included for each incubator to

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 1.4V button cells give 350hrs continuous operation; cope with 160 – 260 V.
size 148x28x15mm, 85g (approx.).
Resolution: 0.01
MV range: ± 1999
Accuracy: ± 0.01
No. of Digits: Three
18 Incubators Laboratory incubators: (One each) 3 The apparatus should confirm
(1) Temp.: 25 degree C (BOD Incubator) to Indian Standard Institution
Specifications- Stainless steel make of SS-304 grade, Guidelines with latest
full length inner plexi-glass door. amendments in Indian
Castor wheel mounted tie easy movability Standard Specification for
CFC free High efficiency refrigeration system Incubators or equivalent
mounted at bottom, proper air circulation for National or International
uniformity. Standards covering Markings,
Temperature Range: +5oC to 50oC, +0.5oC resolution tests and Safety requirements
Temperature Control: Digital Control, Voltage regulators of
Microprocessor based controller for mains, heating, appropriate rating to be
and cooling with separate indicator lights. included for each item to cope
Accuracy of Temperature: +0.5oC with 160-260 V.
Power: 230 volts, 50Hz AC, Mains single phase.
Size: (of inner chamber):- 700(W)x900(H)x650(D)
mm approx., Capacity:- 15 cu. ft. with 3 shelfs, made
of stainless steel and inner illumination with sleek
fluorescent tubes.
Suitable Voltage stabilizer:
(2) Temp: 37 degree C (Temp. variation ± 0.5 oC)
(Memmert type),
(3) Temp: 42 degree C (Temp. variation ± 0.5 oC)
(Memmert type)
Specifications (for 2 & 3):
(i) Stainless steel make, inner full length plexi-glass
door.
(ii) Triple wall with special grade glass wool
insulation
(iii) Long lasting stainless steel tabular heaters with
fins
(iv) Temperature range, ambient +5oC to 80oC X
±0.1oC resolution Controller/Digital indicator for
13
 Temperature
Size in mm approximately (of inner chamber):-
700(W)x900(H)x650(D), , Capacity:- 15 cu. ft. and
door swing 65 cms.

Sl. No. Item Technical Specifications Quantity per Remarks

Consignee
Schedule IV
1 Ice Box Equipment quoted should
Double insulated wall with polyurethane foam, non-
3 comply with Indian
rust, non-leak and crack-proof, easy to clean, made of
Standards Institutions
durable polystyrene, with at least two ice packs, for
Guidelines or any other
storage of sputum specimens for at least six hours,
National or International
Size (internal) =10” x 10” x 10”.
Guidelines.
2 Refrigerator Vertical, capacity 165lts or more, frost free, CFC free, House hold refrigerator
single door, 220V AC, 50 HZ. 1 required.
Equipment quoted should
comply with Indian
Standards Institutions
Guidelines or any other
National or International
Guidelines. Voltage
regulator of appropriate
rating to be included to
cope with 160-260 V.
3 Fast flame Bunsen burner Stainless Steel, for use with
Bunsen burner
natural and cylinder gases, 15cms height, head 5
for LPG gas
diameter 13 mm, Nickel plated with stopcock.
4 Stainless steel rod with heat resistant handle holder; 6''
Loop holders in length; with screw arrangement to hold firmly 24 12
SWG Nichrome wire inoculation loop.
5 Nichrome wire, 24 Standard Wire Gauge (SWG) or
Loop Wire 10 metres
0.022 inches or 0.559 mm thickness SWG.
6 Diamond 6" holder with artificial diamond (hard stone) 6
marker Pencil embedded at one end with screw cap to mark on
microscope glass slides.

7 Grease marking Marking pencil MPS, Blue or red coloured, 8" length, 8
14
pencil to write on glass ware/metal surfaces.

15
 1 Equipment quoted should
8 Hot plate Hot plate, heating range 40-110 degree C, 220volt comply with Indian
with temperature controller, 1ft x 1ft square flat plate, Standards Institutions
chemical and scratch resistance ceramic plate, Guidelines or any other
temperature uniform across the plate, spill trough to National or International
deflect spills away from electronic and control knobs Guidelines. Voltage
with a LCD/LED indicator, hot indicator light regulator of appropriate
whenever hot plate is above 50 degrees. rating to be included to
cope with 160-260 V.
9 Stainless Steel, 5Lts. Capacity, Ht. 12” x Diameter 6”, 6
Discard bins
with lid.
10 Stainless Steel, 6” in length, serrated blunt tips. 6
Forceps
straight.
11 Cotton Absorbent, each roll of 0.5 kg. 10 rolls
12 Stop Watch Mechanical stop watch, for a maximum of 60 minutes 3 Equipment quoted should
duration with timer and alarm, dial diameter :10 cms, comply with Indian
thickness: 5 cm. Standards Institutions
Accuracy: 1/5 sec Guidelines or any other
National or International
Guidelines.
13 Stainless steel
Stainless Steel, Size: 15” length, 10” Width, 3” Depth. 2
tray
14 Wire rack Stainless Steel wire carrying rack, sturdy welded steel 10
wire (of approximately 0.25" diameter) construction,
used to hold 20 McCartney bottles(10 bottles x2 rows)
of approximately 28.5 cms external diameter and 82
cms height, 50 gm. Weight each bottle; rack should be
autoclavable, resist rust, corrosion, solvents, and acids.
Entire rack size approximately 13" L x 2.5" B x 2.5"
height. Racks should have a base made of steel wire
mesh of 0.5" L x 0.5" B, The dimensions of top wire
mesh 1.25" L x 1.25" B.

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15 Filter paper Filter paper No. 1, diameter:- 12.5 cms for filtering
carbol fuchsin using a small funnel of 10cm maximum
120 packs (of
diameter, Smooth on one side and rough on the other
100 each)
side, water permeable

16 Stainless Steel Stainless Steel, 60 Litres capacity, 18 gauge 2

Bucket with lid
17 To hold approximately 200 universal containers 3
(McCartney bottles) for autoclaving, Aluminium
Perforated welded mesh. Size: 480mm x 280mm Compatible with
Baskets the autoclave inner chamber of diameter of Height 500
mm x Diameter 300 mm, with two hinged handles at
the top
18 Test Tube Rack Aluminium, S-shaped, 9” x 3” with holes size of 6
20mm, storage capacity:30 tubes.
19 Stainless Steel Stainless Steel, 6” length, surgical, open shanks, 3
Scissors straight with fine sharp point.
20 Spatula Nickel with spoon end size:180mm; 6
9”length
21 Clips (50mm) Clip Mohr, Nickel plated Iron, thick quality, round 2
edges, length 50mm.
22 Clips (70mm) Clip Mohr, Nickel plated Iron, thick quality, round 2
edges, length 70mm
23 Knives Stainless Steel, with handle for cutting glass tubing ; 6
pocket size knife: 10cms.
24 Cork Borers Stainless Steel, insets with rod separate handle fitted to 2
each set of 1-12, 5-19mm
25 Boxes (96mm) Stainless Steel for holding ten 4”(96mm.) glass petri 2
dishes for sterilization.
26 Boxes Cylindrical, Stainless Steel for sterilizing pipettes; full 3
(46cms x 4cm) length of 46cms length x 4 cms diameter with a tight
closing lid. Sterilisation canister for glass pipettes.
27 Stainless Steel Stainless Steel, with pouring spout, Size: 20” x 12” x 4
Trays 2.5”

28 Stainless Steel Stainless Steel, capacity 2 ½ Litres 4

Bowl
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29 Stainless Steel Stainless Steel, 12 cm diameter, stack of 8 cm length,1 2
Funnel cm. bore size.
30 Drying Rack Stainless Steel, to drain microscope slides after 6
staining, each rack to accommodate at least 16 slides,
approximate dimensions 350 x 180 x 5 mm.
31 Bucket with lid. Stainless Steel, capacity 4 Gallons 6
32 Mug Stainless Steel, 1 pint size 4
33 Brown Paper 115 cm.x 75 cm. size sheets 75 sheets
for packing
34 Rubber Bands All assorted sizes 1/2kg
35 Dissecting Stainless Steel, serrated tips 14cms length, blunt edges, 5
Forceps straight.
36 Air 1. 5 ton capacity, Window A-C, 3 Equipment quoted should
Conditioners number required is three: one each for primary comply with Indian
culture & DST room, media room and culture reading Standards Institutions
room Guidelines or any other
National or International
Guidelines. Voltage
regulators of appropriate
rating to be included for
each item to cope with
160-260V.
37 Non- absorbent Non-absorbent cotton rolls of 1/2 kg each, surgical 4 Kg (roll of
Cotton grade ½ kg each)

38 Wire Racks to Stainless Steel wire carrying rack, sturdy welded steel 50
hold 150 wire (of approximately 0.25" diameter) construction,
Universal used to hold 150 McCartney bottles (of approximately
Containers 28.5 cms external diameter and 82 cms height, 50 gm.
(McCartney Weight each), autoclavable, should resist rust,
bottles) corrosion, solvents, acids. Entire rack size
approximately 22.5" L x 15" B x 2.5" height. Racks
should have a base made of steel wire mesh of 0.5" L x
0.5" B, The dimensions of top wire mesh 1.25" L x
1.25" B.
39 Wire gauge Iron mesh of 1mm thick wire for Tripod stand, 15cms 2
with non- x 15 cms, with 10cms diameter asbestos in the centre,
asbestos pad at Size: 6” x 6”.
18
 the centre.
40 Tripod stands Iron Tripod stand made of 7mm diameter rod, 2
Minimum distance between any two legs 15cms, height
23cms, stand of equilateral triangle of 10cms on each
side.
41 Stainless Steel Two 5mm diameter stainless steel rods of length 2ft 6 sets
Rods for each welded at both ends with small stainless steel rods
staining (set of of 5 cms length and 5 mm diameter, to obtain a
two rods). rectangular rack for placing on wash basin during
staining of microscope slides.
42 Industrial Acid & alkali proof, Rubber, Anti-static, to cover at 1 pair
Gloves least ¾th of forearm.
43 Lens Paper Soft microscope lens cleaning tissue, 4” x 6” booklet, 50 booklets
each booklet containing 100 sheets. (each
containing
100 sheets)

19
Sl. No Item Technical Specifications Quantity Remarks
per
Consignee
Schedule V (Glassware and related items)
1. Slides Glass microscope slides; 76mm x 26mm x 1.3mm, 144 packs
plain, one pack of 50 slides. (50 slides
each)
2. Glass ware for stain 1 Lt. Capacity. 10
preparation - Round
(flat bottom) flask
3. Glass ware for stain Graduated 100mI cylinder 4
preparation -
Measuring cylinder
4. Glass ware for stain Graduated 500mI cylinder 4
preparation -
Measuring cylinder
5. Containers for Glass stoppered bottles, 250ml capacity. 8
stains
6. 250ml capacity built-in stem all polypropylene; leak 8
Dropper Bottles
proof screw cap made of polypropylene.
7. Glassware for Autoclavable and dry heat resistant, flat bottom, round
media preparation flask. Extended neck (not Conical flask) 3
i) 3 lts. Flasks
8. ii) 1 Lts. Flasks Autoclavable and dry heat resistant, flat bottom,
12
conical flask
9. iii) 1 ml Pipettes Autoclavable and dry heat resistant, graduated 1/10
15
(0.1ml graduation)
10. iv) 2ml Pipettes Autoclavable and dry heat resistant, graduated 1/20
15
(0.1ml graduation)
11. v) 5ml Pipettes Autoclavable and dry heat resistant, graduated 1/20
15
(0.1ml graduation)
12. vi) 10ml Pipettes Autoclavable and dry heat resistant, graduated 1/20
15
(0.1ml graduation)

20
13. vii) 500ml Autoclavable and dry heat resistant, graduated 1/20 (0.1ml
12
Beakers graduation)
14. viii) 250ml Autoclavable and dry heat resistant, graduated 1/20 (0.1ml
12
Beakers graduation)
15. Bijou bottles Borosilicate or corning glass of 5 ml capacity with screw 250
caps of Aluminium with rubber liners suitable for Bijou
bottles, leak proof.

16. Glass beads Acid washed, 3mm size; for dispersing the clumps of 500gms
micro-organisms by vortexing or blending.
17. 1 ml Pipette, 1ML, 1 in 10 graduation, Low-density polyethylene, 50
sterile sterile, graduated, each packed separately to maintain (boxes)
disposable sterility, 100 pipettes per box .
18. 5 ml Pipette, 5ML, 1 in 10 graduation, Low-density polyethylene, 50
sterile sterile, graduated, each packed separately to maintain (boxes)
disposable sterility, 10 pipettes per box.
19. 10 ml Pipette, 10ML Low-density polyethylene, sterile, graduated, each 50
sterile packed separately to maintain sterility, 10 pipettes per box. (boxes)
disposable
20. Rubber teats Latex rubber teats for Pasteur pipettes. Capacity 2.5ml ; 6
neck bore 6.5mm.
21. Rubber bulbs Pear shaped moulded rubber for use with pipettes. Neck 6
bore approx 4mm; 15ml capacity.
22. Retort stand 12” x 6”stand with 30” rod. 2
base
23. Retort ring Ring 3.25” diameter; arm length 13 cms 1
plain rod
24. Retort ring Ring of 4” diameter; arm length 13 cms 1
plain rod
25. Retort ring Ring of 6” diameter; arm length 13 cms 1
plain rod
26. Clamps Brass or cadmium plated for retort stand. 2
27. Boss heads Brass or cadmium plated for holding retort ring. 2
28. Test tube White bristles on tinned iron white-fan shaped ends for test 2
brushes tube size 3/8 “ x ½ “ dozens
29. Cylinder Nylon bristles with twisted steel handle. Arm length 40 1 dozen
brushes cms.
21
30. Nylon brush, Nylon bristles with twisted steel handle; Sizes 0.75” to 1”. 1 dozen
large for test
tube
31. Brush for flask Nylon bristles with twisted steel handle for washing 250ml 1 dozen of
-3 lit flask; Two arm length (15, 40 cms). each arm
length.
32. Mortar pestles Heavy glass, external diam. 4”, capacity 8 oz. 1
33. Beakers Glass ,autoclavable & heat resistant ; with spout, capacity 2
250ml.
34. Beakers Glass ,autoclavable & heat resistant , capacity 100ml. 2
35. Beakers Glass ,autoclavable & heat resistant , capacity 500ml. 2
36. Beakers Glass ,autoclavable & heat resistant , capacity 1000ml. 1
37. Test tubes Glass, autoclavable & heat resistant, standard rimless, 150 100
x 16 mm with screw caps.
38. Funnels clear Plain diameter: 5 cm 1
glass
39. Funnels clear Plain diameter: 10 cm 2
glass
40. Funnels clear Plain diameter: 18 cm 2
glass
41. Volumetric Whiteline graduation with glass stopper, 50ml capacity 1
Flask
42. Volumetric Whiteline graduation with glass stopper, 100ml capacity 1
Flask
43. Volumetric Whiteline graduation with glass stopper, 1000ml capacity 1
Flask
44. Boxes for For storing 100 Slides, Plastic storage boxes, approximate 15
storing slides dimensions: 8.75" (22.2 cm) x 6.75"(17.15 cm) x 1.3"(3.3
cm).
45 Spare Caps for Screw caps of Aluminium with rubber liners suitable for
Bijou bottles Bijou bottles. 500

22
 Schedule VI (Chemicals, Reagents etc)
Total
Quantity
per
S.no Item Technical specifications consignee Remarks
Chemicals Formula:K2Cr207, Formula Wt:
1 Potassium Di chromate 294.2, Minimum assay:99.5% 1 Kg
Household disinfectant, containing ohenolic
compounds such as monochlorophenol, coal tar
Phenolic compounds acid, oils & Emulsifiers etc. The approx content
2 containing disinfectants of phenolic compounds should be at least 40%. 50 Ltrs
Chemical name :Pararosaniline hydrochloride,
chemical structure: C20H20ClN3 mol
wt:337.86'Dye content: Approx.85%-88%(dye
content must be mentioned), Colour: Metallic
3 Basic Fuchsin green. 500 gms
Chemicals name :Sulphuric Acid, Formula Wt:
98.08, Specific Gravity 1.84 Minimum
4 Sulphuric Acid Assay:98% 10 Ltrs
Chemicals name:Malachite green Hydro
Chloride, Chemical structure:C23H25CIN2,
Molecular Wt.=364.9, Colour: Green, Dye
content: 80%+9% Solubility: both in water &
5 Malachite Green alcohol, Stability : More than one year. 150 gms
Chemicals name:Methyl thionine chloride,
Chemical structure:C16H18CIN3S, Molecular
Wt:319.9, Dye content:Approx 82%(dye content
6 Methylene Blue must be mentioned) 100 gms
7 Auramine C17H22ClN3, mol wt: 303.84 250 gms
Chemical name: Phenol, Chemical structure:
C6H5OH, Molecular Wt: 94.11, Melting point:
8 Phenol 40°C+2, purity: 99.5% 5 Kgs
15 Ltrs

Concentrated Hydro Chloric Acid, Molecular Wt:

9 Hydrochloric Acid 36.46 Specific Gravity 1.18
23
10 Potassium Permanganate KMnO4, Formula Wt:158 500 gms
Refractive index of 1.48. It should be colourless,
odurless, transparent, free from fluorescene in
day light with relative density of 0.827 to 0.890,
Liquid Paraffin (Heavy viscosity of 110 to 230 m Pa s., specific gravity
11 Grade) of 0.76-0.78 at 15.5°C 3.5 Ltrs
12 N-acetyl L-Cysteine Chemical structure:C5H9NO3S, mol wt: 163.2 25 gms
Chemical name: Trisodium citrate dihydrate, AR
gradeChemical structure:Na3C6H5O7.2H,O,
13 Sodium Citrate Molecular Wt (FW):294.10, Purity: 99% 500 gms
Potassium Di Hydrogen ortho Phosphate
KH2PO4, Molecular Wt:=136.1Minimum
14 Potassium phosphate assay=99% 1 Kg.
15 Magnesium Sulphate MgSO4.7H2O; mol wt 246.48; purity:99.5% 1Kg
TriBasic ; C12H10Mg3O14.9H2O mol wt:
16 Magnesium Citrate 613.28 500 gms
L- asparagine L-Asparagine monohydrate ; C4H8N23.H2O
17 monohydrate mol wt: 150.14 1 Kg
Formula: CH2OHCHOHCH2OH mol wt: 92.1;
18 Glycerol purity 99.5% 3 Ltrs
Formula:NaOH Formula Wt:40 Minimum Assay
19 Sodium hydroxide pellets :98% 1 Kg
Chemical name: Cetyl Pyridinium Chloride;
20 Cetyl Pyridinium chloride Chemical structure: C21H38ClN.H2O / 358 500 gms
Chemical name: sodium chloride chemical
structure :NaC1 Molecular Weight:58.44
21 Sodium Chloride Minimum assay:99.9% 1 Kg

Dihydrostreptomycin
22 sesquisulphate sigma D-7253 5 gms
Isonicotinic acid
23 hydarazide Sigma I -3377 5 gms
24 Rifampicin Sigma R-3501 5 gms
Ethambutol
25 dihydrochloride Sigma E-4630 25 gms pack

24
 Chemical Name Para Nitro benzonic acid
Formula Wt: 167.12, Min assay =99% 100 gms
26 PNB C6H4COOHNO2 pack
100 gms
27 Cyanogen bromide CNBr- Cisco Research Lab:mol wt:105.9 pack
50 gms
powder
28 O-Tolidine A.R Dimethyl benzidine ; C14H16N2 form wt: 212.3 pack
AR or GR grade, assay : 29-32% solution, FW-
29 Hydrogen peroxide 34.01 1.5 Ltr
Chemical name:
Polyoxyethylenesorbitanmoonooleate. AR or GR
30 Tween-80 Grade, Density=1.06-1.09g/ml 500 ml
COOHCOOH.2H2; mol wt:126.07 ; purity
31 Oxalic acid(pure) 99.8% 500 gms
32 Absolute alcohol Ethanol 1 Ltrs
33 Liquor ammonia LR/GR Grade 1 Ltr
Formula Na2CO3 Formula Wt: 106 Minimum
34 Sodium carbonate asay :99% 500 gms
N-N-dimethyl formamide HCON(CH3)2 mol wt: 73.09
35 500 ml
Formaldehyde HCHO; mol wt: 30.03; wt per ml at 20oC=1.075-
1.095 g; methanol content=10-15%, Assay
36 (acidimetric) as HCHO 37-41% 2.5 Ltrs

General requirements:-
1. All equipment quoted should comply with Indian Standards Institutions Guidelines or any other National or International Guidelines.
2. All equipment which does not have built in voltage stabilizer should be supplied with appropriate voltage stabilizers.
3. All the chemicals should have the Hazard Communication Standard from Chemical manufacturers and Material Safety Data Sheets

(MSDS) for each chemical item should be provided to the purchaser of the product at the time of the order and thereafter, anytime the
MSDS is significantly revised. Also, all the chemicals should have their detailed specifications, potency, date of manufacture, date of
expiry, batch no. and precautions for use mentioned on the containers.

25