The document lists and describes various pieces of equipment commonly used in microbiology laboratories, including an alcohol lamp, analytical balance, applicator sticks, aspirator, autoclave, beakers, cotton swabs, droppers, Erlenmeyer flasks, fecal incubators, glass slides, graduated cylinders, hot plates, microscopes, petri dishes, pipettes, stirring rods, stop watches, test tubes, test tube racks, test tube tongs, thermometers, transfer loops, and water baths.
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Activity 1
The document lists and describes various pieces of equipment commonly used in microbiology laboratories, including an alcohol lamp, analytical balance, applicator sticks, aspirator, autoclave, beakers, cotton swabs, droppers, Erlenmeyer flasks, fecal incubators, glass slides, graduated cylinders, hot plates, microscopes, petri dishes, pipettes, stirring rods, stop watches, test tubes, test tube racks, test tube tongs, thermometers, transfer loops, and water baths.
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BAGUIDUDOL, JOY P.
Jan 14, 2023
ACTIVITY No. 1 – MICROBIOLAB APPARATUS AND EQUIPMENT
Alcohol Lamp
An alcohol burner or spirit lamp is a piece of
laboratory equipment used to produce an open flame. It can be made from brass, glass, stainless steel or aluminum.
Analytical Balance
Analytical balances are precision measuring
instruments used in quantitative chemical analysis, to determine the mass of solid objects, liquids, powders, and granular substances. Today, electronic balance uses the principle of magnetic force restoration, offering readability up to .0001 g.
Applicator Stick
Laboratory applicator sticks are needed for
numerous laboratory applications, including specimen transfers and mixing of solutions.
Aspirator
An aspirator is also known as an eductor-jet
pump or a filter pump. This is a device that is able to produce a vacuum by using the venturi effect. In an aspirator, there is a fluid (either liquid or gaseous) that flows through a narrowing tube. Once the tube starts narrowing, the fluid's speed is able to increase. This is because of the aforementioned venturi effect. The pressure eventually decreases and from here onwards, the vacuum is taken from this point. Autoclave
An autoclave is a machine used to carry out
industrial and scientific processes requiring elevated temperature and pressure in relation to ambient pressure and/or temperature. Autoclaves are used before surgical procedures to perform sterilization and in the chemical industry to cure coatings and vulcanize rubber and for hydrothermal synthesis. Industrial autoclaves are used in industrial applications, especially in the manufacturing of composites. Beaker
A beaker is generally a cylindrical container
with a flat bottom. Used for various purposes —from preparing solutions and decanting supernatant fluids to holding waste fluids prior to disposal to performing simple reactions. Low-form beakers are likely to be used in some way when performing a chemical experiment. Beaker Tong
Beaker tongs are welded steel tools used to
lift a hot beaker from a furnace or for other items which cannot be handled with bare hands. Beaker tongs may also be used to prevent any inaccuracies regarding mass measurements as any moisture or oil will affect the readings and accuracy.
Cotton Swab
Disposable cotton swabs manufactured of
USP grade purified cotton firmly attached to one end of wood shaft. Useful in bacteriology labs for inoculating broths, plated media or for obtaining culture specimens. Also useful for cleaning delicate instruments.
Dropper
An eye dropper, also called Pasteur pipette or
simply dropper, is a device used to transfer small quantities of liquids. Erlenmeyer Flask
Erlenmeyer flasks are conical flasks with wide
flat bottoms and tapered necks. The flasks are designed for a variety of laboratory liquid handling applications, including pouring, mixing, storage, and suspension cell culture. Fecal Incubator
Incubate total and fecal coliforms, fecal
streptococci and other microorganisms Complies with Standard Methods requirements View samples through clear plastic inner door without opening, preventing temperature fluctuations Compact lightweight portable unit for field use. Glass Slide
A microscope slide is a flat piece of glass
measuring 75 x 25mm with a thickness of 1mm. Many slides have a frosted section at one end, on which patient details can be written to ensure that slides from different patients are not mixed up. Millions of glass slides are used in pathology laboratories every day. Graduated cylinder
A Measuring Cylinder/graduated cylinder/
cylinder measuring/mixing cylinder is a piece of laboratory apparatus used to measure the volume of liquids, chemicals, or solutions during the lab’s daily work. Graduated cylinders are more precise and accurate than the common laboratory flasks and beakers. Hot Plate
Hot plates are used in a laboratory as a heat
source that can uniformly heat solutions and materials. They are considered much safer than traditional Bunsen burners because there is no open flame involved, just a heated plate. Microscope
A microscope is an instrument that is used to
magnify small objects. Some microscopes can even be used to observe an object at the cellular level, allowing scientists to see the shape of a cell, its nucleus, mitochondria, and other organelles. Petri Dish
A Petri dish is a shallow transparent glass or
plastic cylindrical lidded dish, used to contain a thin layer of agar on which to grow bacteria and fungi. Millions of Petri dishes are used in microbiology labs every year.
Pipettes
A pipette is a laboratory instrument used to
measure out or transfer small quantities of liquid, in volumes of milliliters (mL), microliters (μL).
Stirring Rod
The function of a stirring rod is to mix or stir
liquids. Mixing will speed up any reaction that takes place in the test tube or flask. A glass stirring rod can also be used for decanting (pouring off) a liquid from a precipitate or sediment. The rods are thicker and longer than a drinking straw and made from a special laboratory glass called borosilicate. The thickness of the rod makes it unaffected by cold or heat. Stop Watch
A laboratory stopwatch measures time
intervals using specific activation and deactivation points. Analog or mechanical stopwatches are typically controlled by two buttons. The first button starts the timer and the second stops the measurement, and the elapsed time is displayed.
Test Tube
A test tube is a relatively slim glass or plastic
vessel with a rounded bottom. They are designed to hold small quantities of chemicals and feature a flared lip to make pouring easier. Test tubes can hold liquid or solid chemicals and can be used to contain small chemical reactions. The slimness of the test tube reduces the spread of any vapors that may be produced by the reaction. Test Tube Rack
Test tube racks can be made of wood, metal,
or plastic. Test tube racks are used for holding test tubes upright when they contain chemicals, and also hold test tubes upside down to dry. They can also be used to line up several test tubes to be used as collection containers in a distillation.
Test Tube Tong
The test tube holder is a piece of laboratory
equipment that is used to hold test tubes. They are made out of metal and are used by squeezing the handles to open the other end, and inserting the test tube. Test tube holders are typically used when heating the test tube is necessary, or when caustic materials are being handled. Test tube holders are a staple in most commercial and student laboratories.
Thermometer
These devices are used for the quick
measurement of temperature. It finds its application in laboratories.
Transfer Loop
An inoculation loop (also called a smear loop,
inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. This process is called inoculation.
Water Bath
A water bath is a laboratory equipment that
is used to incubate samples at a constant temperature over a long period of time. Water bath is a preferred heat source for heating flammable chemicals instead of an open flame to prevent ignition.