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Instruments of Pharmacology Lab

The document summarizes several instruments commonly used in experimental pharmacology, including the organ bath for studying drug effects on isolated tissues, the rota-rod for evaluating effects on motor coordination, and Eddy's hot plate and analgesiometer for measuring analgesic drug effects using thermal stimuli. The actophotometer and electroconvulsometer are also described for assessing drug impacts on locomotion, wakefulness, and types of epilepsy respectively.

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Om Prakash Mishra
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8K views3 pages

Instruments of Pharmacology Lab

The document summarizes several instruments commonly used in experimental pharmacology, including the organ bath for studying drug effects on isolated tissues, the rota-rod for evaluating effects on motor coordination, and Eddy's hot plate and analgesiometer for measuring analgesic drug effects using thermal stimuli. The actophotometer and electroconvulsometer are also described for assessing drug impacts on locomotion, wakefulness, and types of epilepsy respectively.

Uploaded by

Om Prakash Mishra
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Experiment No.

02
Aim: To study commonly used instruments in experimental pharmacology.
Reference: Kulkarni.S.K, Handbook of experimental pharmacology, Edition 4th, published
by vallabh prakashan, page no. 2-5.
2

Requirements:
Theory:
1. Student organ bath: The organ bath is a traditional experimental set set-up
up that is commonly
used to investigate the physiology and pharmacology of in vitro tissue preparations.
Perfused tissues can be maintained for several hours in a temperature controlled organ
bath. Typical experiments
riments involve the addition of drugs to the organ bath or direct/field
stimulation of the tissue. The tissue reacts by contracting/relaxing and an isometric or
isotonic transducer is used to measure force or displacement, respectively. From the
experimental results dose-response
response curves are generated (tissue response against drug
dosage or stimulus potency).

2. Rota-rod: Rota-rod
rod test is widely used to evaluate drug effects on motor coordination,
balance and motor learning in rodents. The principle of this test is that rats or mice are first
trained to walk on a rod rotating at a certain speed.
3. Eddy’s Hot Plate: In this method heat is used as a source of pain Animals are
individually placed on a hot plate maintained at constant temperature (55 0C) and the
reaction of animals, such as paw licking or jump response is taken as the end point.

4. Analgesiometer: The application of thermal radiation to the tail of an animal provokes


the withdrawal of the tail by a brief vigorous movement. It is the reaction time of this
movement that was recorded.

5. Actophotometer: Most of the central nervous system acting drugs influence the
locomotor activities in man and animals. The CNS depressant drugs such as barbiturates
and alcohol reduce the motor activity while the stimulants such as caffeine and
amphetamines increase the activity
activity. In other words, the locomotor activity can be an
index of wakefulness (alertness) of mental activity.
6. Electro-convulsometer: Different types of epilepsies, i.e., grand mal, petit mal or
psychomotor type, can be studied in laboratory animals. The maximal electro-shock
(MES)-induced convulsions in animals represent grand mal type of epilepsy. Similarly,
chemo-convulsions due to pentylenetetrazol which produce clonic type of convulsions
resemble petit mal type of convulsions in man. These are the two procedures used to
study convulsions, and to test anticonvulsant drugs in laboratory animals.
7. Histamine chamber
8. Pole climbing method

Result: The commonly used instrument in experimental pharmacology was studied.

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