A DRIAN F LORES L OZADA

Boston, MA 02478  Cell (858) 717-6405  [email protected]

PROFESSIONAL OVERVIEW
Principal Scientist experienced in developing technology for pre-clinical R&D and certification in Translational Science.
Core Qualifications
 Translational Science  EEG (rodent)  Neurodegeneration
 Light Sheet Microscopy  Fluorescence Microscopy  Molecular Biology
 IVIS Optical Imaging  Immunohistochemistry  Viral Vectors
 Short-Wave Infrared Optical Imaging  Small Animal Stereotaxic Surgery  Cell Culture*
 2-Photon Laser Scanning Microscopy  Neuroanatomy  In situ Hybridization
 Confocal Microscopy  Animal Behavior  Drug administration
*Hippocampus, cortex, glia/microglia and HEK’s (engineered to detect neurotransmitters)

PROFESSIONAL EXPERIENCE
LIFE CANVAS TECHNOLOGIES October 2022 – January 2023
Principal Scientist October 2022 – Present
 Business development with primary focus on developing long-term partnerships with pharmaceutical companies.
 Determine how LCT services fit into various pre-clinical pipelines regardless of API delivery. With intent to assist
in pre-IND/IND applications. Applied spatial proteomics on animal model of Alzheimer’s Disease as well as
different tissue types (spine, liver, lung, muscle etc.) from rodents, NHP, and swine. Focus on liver was to use
spatial proteomics identify Hepatocytes, Kupffer Cells, and endothelial cells.
 Project manage projects for different clients and ensure deliverables are provided in a timely manner.
 Lead team to ensure deliverables are provided as promised in SOW and MSA.

PURETECH HEALTH July 2019 – September 2022

Principal Scientist March 2022 – September 2022
 Continued development and implementation of imaging technology to study/quantify surveillance of CNS by
immune cells (resident macrophages, dendritic cells, neutrophils, etc.) in intact preclinical models of
neurodegeneration. Focus was to determine if implanted, engineered, bone marrow derived monocytes could
secrete therapeutic levels of VEGFc.
 Design and develop probes for IVIS to quantify and study immune surveillance status of the CNS (deep cervical
lymph nodes). Our goal was to prevent or reduce age/disease-related decline as evidenced by reduced lymphatic
flow to CNS and lymph nodes.
 Started use of mirror bed on IVIS for simultaneous imaging of dorsal and ventral regions of rodents during tracer
infusion.
 Employ STED microscopy and antibody IgG modifications to characterize nanoparticles and determine location in
chylomicrons.
 Completed Translational Science certification.
 Lead/train team with the implementation of new technology in drug/therapeutic development.

Senior Scientist July 2019 – March 2022

 Developed and implemented imaging technology (2-photon, confocal, SWIR wide-field microscopy, IVIS, etc.) to
study/quantify immune surveillance of system of CNS in an intact preclinical models of neurodegeneration (age-
related, Alzheimer’s, EAE). Imaged cultured monocytes to determine extent of transfection intended to prevent
differentiation into macrophages.
 Utilized western blotting and ELISA to determine amount of mature VEGFc in meningeal tissue of young and old
Adrian Flores Lozada H:(858) 450-9485 C:(858) 717-6405 [email protected] Page | 2

animals.
 Utilized imaging to study mesenteric lymph nodes in vivo with emphasis on determining pharmacokinetics of
prodrug delivery to lymph after oral gavage.
 Delivered tracers/anesthetics/prodrug via different routes (IV, IT, IM, IP, ICV, PO)
 Started Translational Science certification.
 Led/trained team with the implementation of new technology in drug/therapeutic development.

UNIVERSITY OF CALIFORNIA, SAN DIEGO – San Diego, CA 2006 – 2019

Assistant Project Scientist, Step IV (Department of Physics) 2017 – 2019
 Maintained cell cultures (CNiFERs: Cell-based Neurotransmitter Fluorescent Engineered Reporters) as well as
generated dose response curves for neurotransmitters cells that were designed to detect dopamine,
noradrenaline, and acetylcholine.
 Applied stereotaxic surgery to implant various tools utilized for 2 photon intravital imaging (I.e., CNiFERs,
craniotomy window, thin skull window, etc.). Also used stereotaxic surgery for implantation of various types of
electrodes for EEG recordings (e.g., determine location of locus coeruleus).
 Applied 2 photon intravital imaging coupled to brain machine interface to determine extent of volitional control
of dopamine release in cortex.
 Designed and generated viral vectors as well as AAV/lenti/rabies viruses for in vivo use.
 Supervised junior postdocs, grad students, and undergraduates.

Assistant Project Scientist, Step III (Department of Physics) 2015 – 2017

 Designed and implemented a 2 photon intravital imaging prep in vivo to study altered hippocampal activity
resulting from neonatal exposure to nicotine.
 Transfected hippocampal neurons with GCaMP to record cell activity.
 Used stereotaxic surgery for implantation of various types of electrodes for EEG recordings (e.g., determine
location of locus coeruleus).
 Utilized wheel running behavior as a platform to study brain activity related locomotion.
 Implanted CNiFERs into hippocampus to determine patterns associated with noradrenaline release.
 Supervised junior postdocs, grad students, and undergraduates.

Assistant Project Scientist, Step II (Department of Neurobiology) 2013 – 2015

 Determined how neuronal connectivity is altered with neonatal exposure to nicotine.
 Designed a mini-pump based dosing strategy to maintain smokers’ levels of nicotine in developing rodents
 Designed experiments and imaging (fluorescent, confocal, etc.) related to determining nicotinic acetylcholine
receptor involvement in synapse formation.
 Supervised junior postdocs, grad students, and undergraduates.

Assistant Project Scientist (Department of Neurobiology) 2011 – 2013

 Designed and utilized experiments and imaging (fluorescent, confocal, etc.) related to determining nicotinic
acetylcholine receptor involvement in synapse formation.
 Designed viral vectors and generated (RNAis) and transfected primary cell cultures.
 Supervised junior postdocs, grad students, and undergraduates.

Postdoctoral Fellow (Department of Neurobiology) 2006 – 2011

 Designed and utilized experiments and imaging (fluorescent, confocal, etc.) related to determining nicotinic
acetylcholine receptor involvement in synapse formation. Both in vitro (primary cell cultures) and in vivo.
 Cloned (via PCR) M4 muscarinic receptor in chick. Performed western blotting to determine which antibodies
were most useful for synaptic proteins.
 Supervised junior postdocs, grad students, and undergraduates.
Adrian Flores Lozada H:(858) 450-9485 C:(858) 717-6405 [email protected] Page | 3

EDUCATION
Translational Science Certificate University of California, San Diego Extension, San Diego, CA USA (2022)
Altman Clinical and Translational Research Institute

Ph.D., Biological Sciences Abo Akademi University, Abo, Finland (2005)

M.S., Biological Sciences California State University, Long Beach, Long Beach, California, USA (2000)

B.S., Biological Sciences University of California, Irvine, Irvine, California, USA (1993)

Thesis/Dissertation: Modulation of Central Histaminergic System in Pathology and Plasticity

FELLOWSHIPS AND AWARDS

v Dean's Honor List1991-1993
v Cum Laude 1993
v Minority Biomedical Research Support (MBRS) Program Fellow, Department of Biology, CSU Long Beach, CA
1996-2000
v Magnus Ehrnrooth stipend (salary grant)2002
v Oskar Oflunds stipend (travel grant)2002, 2003
v Young Investigators Award, 2nd prize sponsored by Abbott Laboratories and a travel bursary sponsored by
Novo Nordisk at the XXXIInd annual meeting of the European Histamine Research Society2003
v Alcohol Research Foundation (salary grant)2003, 2004, 2005
v Minority supplement grant to NS35469 (Grant: R01NS035469-10S1; PI: Darwin Berg), 11/01/2005-
02/28/2008
v NIH Ruth L. Kirschstein National Research Service Award (NRSA) Award
v 5F32DA023748-(Proposal Title: Nicotinic Regulation of Hippocampal Synapse Formation; PI: Adrian F. Lozada)
2007-2009

PUBLICATIONS

Jayne BC, Lozada AF, Lauder GV. 1996. Function of the dorsal fin in bluegill sunfish: Motor patterns during four
distinct locomotor behaviors. J Morphol. 228(3): 307-326.

Lozada A, Lintunen M, Karlstedt K, Panula P. 2002. Growth associated protein-43 (GAP-43) and L-histidine
decarboxylase (HDC) expression levels in tuberomammillary (TM) nucleus of rats with unilateral striatal lesions.
Inflamm Res. Apr;51 Suppl 1:S42-3.

Sallmen T, Lozada AF, Beckman AL, Panula P. 2003. Intrahippocampal histamine delays arousal from hibernation.
Brain Res. Mar 21;966(2):317-20.

Sallmen T, Lozada AF, Anichtchik OV, Beckman AL, Panula P. 2003. Increased brain histamine H3 receptor expression
during hibernation in golden-mantled ground squirrels. BMC Neurosci. Sep 24;4:24.
Adrian Flores Lozada H:(858) 450-9485 C:(858) 717-6405 [email protected] Page | 4

Sallmen T, Lozada AF, Anichtchik OV, Beckman AL, Leurs R, Panula P. 2003. Changes in hippocampal histamine
receptors across the hibernation cycle in ground squirrels. Hippocampus. 13(6):745-54

Lozada AF, Aarnisalo AA, Karlstedt K, Stark H, Panula P. 2004. Plasticity of histamine H3 receptor expression and
binding in the vestibular nuclei after labyrinthectomy in rat. BMC Neurosci. Sep 10;5:32.

Lozada A, Maegele M, Stark H, Neugebauer EM, Panula P. 2005. Traumatic brain injury results in mast cell increase
and changes in regulation of central histamine receptors. Neuropathol Appl Neurobiol. Apr;31(2):150-62.

Lozada A, Munyao N, Sallmen T, Lintunen M, Leurs R, Lindsberg PJ, Panula P. 2005. Postischemic regulation of central
histamine receptors. Neuroscience. 136(1):371-9.

Michelsen KA, Lozada A, Kaslin J, Karlstedt K, Kukko-Lukjanov TK, Holopainen I, Ohtsu H, Panula P. 2005. Histamine-
immunoreactive neurons in the mouse and rat suprachiasmatic nucleus. Eur J Neurosci. Oct;22(8):1997-2004.

Bakker RA, Lozada AF, van Marle A, Shenton FC, Drutel G, Karlstedt K, Hoffmann M, Lintunen M, Yamamoto Y, van
Rijn RM, Chazot PL, Panula P, Leurs R. 2006. Discovery of naturally occurring splice variants of the rat histamine H3
receptor that act as dominant-negative isoforms. Mol Pharmacol. Apr;69(4):1194-206.

Lozada A, Karlstedt K, Panula P, Aarnisalo AA. 2006. Unilateral labyrinthectomy induced changes on GDNF receptor
complex proteins in the rat medial vestibular nuclei. J Vestib Res. 16(4-5):171-177

Bongers G, Sallmen T, Passani MB, Mariottini C, Wendelin D, Lozada A, Marle AV, Navis M, Blandina P, Bakker RA,
Panula P, Leurs R. 2007. The Akt/GSK-3beta axis as a new signaling pathway of the histamine H(3) receptor. J
Neurochem 2007 Oct;103(1):248-258.

Campbell NR, Fernandes CC, John D, Lozada AF, Berg DK. Nicotinic control of adult-born neuron fate. Biochem
Pharmacol. 2011 Oct 15;82(8):820-827.

Lozada AF, Wang X, Gounko NV, Massey KA, Duan J, Liu Z, Berg DK. Glutamatergic Synapse Formation is Promoted by
7-Containing Nicotinic Acetylcholine Receptors. J Neurosci. 2012 May 30;32(22):7651-7661.

Lozada AF, Wang X, Gounko NV, Massey KA, Duan J, Liu Z, Berg DK. Induction of Dendritic Spines by 2-Containing
Nicotinic Receptors. J Neurosci. 2012 Jun 13;32(24):8391-8400.
Fernandes CC, Lozada AF, Berg DK (2014) Nicotinic signaling in development.  In Nicotinic Receptors.  R Lester and P
Kenny, eds. Springer Science+Business Media, New York, pp. 115-135.

Duan JJ, Lozada AF, Gou CY, Xu J, Chen Y, Berg DK. Nicotine recruits glutamate receptors to postsynaptic sites. Mol
Cell Neurosci. 2015 Sep;68:340-9.

Romoli B, Lozada AF,Sandoval IM, Manfredsson FP, Berg DK, Dulcis D (2018) Neonatal nicotine exposure primes
midbrain neurons to a dopaminergic phenotype and increases adult drug consumption. Biological Psychiatry 2019
April 24; 86(5); 344-355.

Mercer Lindsay N, Knutsen PM, Lozada AF, Gibbs D, Karten HJ, Kleinfeld D. Orofacial movements involve parallel
corticobulbar projections from motor cortex to trigeminal premotor nuclei. Neuron 2019 November 20; 104(4); 765-
780.
Adrian Flores Lozada H:(858) 450-9485 C:(858) 717-6405 [email protected] Page | 5

Foo C, Lozada A, Aljadeff J, Li Y, Wang JW, Slesinger PA, Kleinfeld D. Reinforcement learning links spontaneous
cortical dopamine impulses to reward. Current Biology 2021 July 23; https://doi.org/10.1016/j.cub.2021.06.069
Adrian Flores Lozada H:(858) 450-9485 C:(858) 717-6405 [email protected] Page | 6

PRESENTATIONS
Sallmen T, Beckman AL, Lozada AF, Leurs R, and Panula P. (2001) Brain histamine in the regulation of mammalian
hibernation. XXXth Annual Meeting of the European Histamine Research Society, Abo Akademi University, Abo,
Finland

A. Lozada, M. Lintunen, K. Karlstedt, and P. Panula (2001) Changes in the histaminergic neuronal system due to kainic
acid induced lesions in striatum. XXXth Annual Meeting of the European Histamine Research Society, Abo Akademi
University, Abo, Finland

A. Lozada, Sallmen, T., Nzau, M., Lintunen, M., Karlstedt, K., Lindsberg, P., and Panula, P. (2002) Use of
[125I]iodoaminopentidine to detect binding densities of histamine H2 receptor subtype in rat model of global
ischemia. Neuroscience Finland, Pharmacity Auditorium, Abo, Finland

AA Aarnisalo, A Lozada, K Karlstedt and P Panula (2002) Expression pattern of H3-receptor subtypes in the vestibular
nuclei of the rat. Inner Ear Biology (IEB), Liege, Belgium

A. Lozada, M. Maegele, H. Stark, E.M.A. Neugebauer, and P. Panula (2003) Histamine H3 Receptor Binding and
Expression Levels in Experimental Traumatic Brain Injury (TBI): Comparison to H1 Receptor Expression Levels and
Expression Levels of Oxidative Stress Markers. XXXIInd Annual Meeting of the European Histamine Research Society,
Amsterdam, The Netherlands

R.A. Bakker, A. van Marle, A. Flores Lozada, S.M. Meusburger, K. Karlstedt, K. Retra, M. Lintunen, F. di Summa, M.
Hoffmann, G. Drutel, M.J. Smit, H. Timmerman, P. Panula, and R. Leurs (2003) Cloning and functional identification of
novel rH3 receptor isoforms. XXXIInd Annual Meeting of the European Histamine Research Society, Amsterdam, The
Netherlands

Kimmo A. Michelsen, Adrian Lozada, Kaj Karlstedt, Timothy W. Lovenberg, and Pertti Panula. (2003) The
histaminergic neuron system and GAD expression in H3 knock-out mice. 33rd Annual Meeting of the Society for
Neuroscience, New Orleans, Louisiana, USA

M. Lintunen, A. Lozada, J. Nissinen, A. Pitknen, and P. Panula (2004). Histamine, a Bad Boy or a Good Girl in Kainic
Acid-Induced Epilepsy? XXXIIIrd Annual Meeting of the European Histamine Research Society, Cologne, Germany.

A. Lozada, K. Michelsen, K. Karlstedt, Y. Yamamoto, and P. Panula (2004). Expression of the Histamine H4 Receptor
During Rat Development. XXXIIIrd Annual Meeting of the European Histamine Research Society, Cologne, Germany.

K. Karlstedt, A. Lozada, K. Michelsen, Y. Yamamoto, and P. Panula (2004). Expression of the Histamine H4 Receptor
During Rat Development. 34th Annual Meeting of the Society for Neuroscience, San Diego, California, USA

K.A. Michelsen, A. Lozada, J. Kaslin, K. Karlstedt, T. Kukko-Lukjanov, I. Holopainen, H. Ohtsu, and P. Panula (2005).
Histamine immunoreactivity in mouse and rat suprachiasmatic nucleus neurons. 35th Annual Meeting of the Society
for Neuroscience, Washington, D.C., USA

A.F. Lozada, Z. Liu, D. K. Berg (2007). Nicotinic regulation of hippocampal synapse formation. 37th Annual Meeting of
the Society for Neuroscience, San Diego, California, USA

A.F. Lozada, K.A. Hebard-Massey, Z. Liu, N.V. Gounko, D.K. Berg (2008). Nicotinic regulation of non-nicotinic
development in vivo. 38th Annual Meeting of the Society for Neuroscience, Washington, D.C., USA
Adrian Flores Lozada H:(858) 450-9485 C:(858) 717-6405 [email protected] Page | 7

A. Halff, N.V. Gounko, K.A. Massey, Z. Liu, J. Duan, D.K. Berg, A.F. Lozada (2009). Nicotinic Activity Determines the
Number and Location of Glutamate Synapses Formed in Early Postnatal Hippocampus. 39th Annual Meeting of the
Society for Neuroscience, Chicago, Illinois, USA

A.F. Lozada, N.V. Gounko, X.L. Wang, J. Duan, K.A. Massey, Z. Liu, A. Halff, D.K. Berg (2010) Endogenous Nicotinic
Activity Drives Formation of Glutamatergic Networks in the Developing Brain. 40th Annual Meeting of the Society for
Neuroscience, San Diego, California, USA

A.F. Lozada, D. John, D.K. Berg (2013) Nicotine-induced aberrations in the development of neural circuits. 43rd
Annual Meeting of the Society for Neuroscience, San Deigo, California, USA

C. FOO, A. F. LOZADA, E. LACIN, E. AISENBERG, P. A. SLESINGER, D. KLEINFELD (2018) Neuromodulator mediated

learning in a closed loop reinforcement system. Program No. 163.11. 2018 Neuroscience Meeting Planner. San Diego,
CA: Society for Neuroscience, 2018. Online.

B. ROMOLI, A. F. LOZADA, I. M. SANDOVAL, F. P. MANFREDSSON, D. K. BERG, D. DULCIS (2018) Neonatal nicotine

exposure primes non-dopaminergic VTA neurons to express dopamine in response to adult nicotine consumption.
Program No. 038.09. 2018 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2018. Online.

A. Lozada (2018) University California San Diego, San Diego, CA. In vivo imaging of neuromodulator transmission in
learning using cell-based neurotransmitter fluorescent engineered reporters. Program No. 348.02. 2018
Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2018. Online.

C. Foo, A. Lozada, Y. Li, J. Wang, P. Slesinger, D. Kleinfeld (2020) Reinforcement learning will Ink spontaneous
dopamine transients to a reward. Virtual Dopamine Conference (http://www.vidaconference.com/speakers.html).
May 21, 2020.