SK-12 Medium Pressure Rotor

Application Book

Application Book
Index

Summary

Application field: Agriculture

Pine Leaves SK12-AG-01
Siliceous Soil With Organic SK12-EN-17
Dried Plant Tissue SK12-AG-02
Urban Wastewater SK12-EN-18
Ground Vegetables SK12-AG-03
Application field: Food/Feed
Maize SK12-AG-04
Ham SK12-FO-01
Hay SK12-AG-05
Agarose SK12-FO-02
Beans SK12-AG-06
Baby Food SK12-FO-03
Lentils SK12-AG-07
BCR 100 Beech Leaves SK12-FO-04
Roots SK12-AG-08
BCR 185 Bovine Liver SK12-FO-05
Tea Leaves SK12-AG-09
BCR 464 Tuna Fish SK12-FO-06

BRC 60 Aquatic Plant SK12-FO-07

Application field: Environmental Bovine Milk SK12-FO-08
Acid Slag SK12-EN-01 Calcium Alginate From SE SK12-FO-09
Dried Biologic Sludge SK12-EN-02 Casein SK12-FO-10
Grass SK12-EN-03 Cheese Ashes SK12-FO-11
Leaves (general method) SK12-EN-04 Chewing Gum SK12-FO-12
Municipal Sludge SK12-EN-05 Chocolate SK12-FO-13
Dried Domestic Sludge SK12-EN-06 Cocoa Powder SK12-FO-14
Pollen SK12-EN-07 Corn Starch SK12-FO-15
US EPA Method 3052 SK12-EN-08 Dried Fish SK12-FO-16
Wastewater SK12-EN-09 Dry Infant Formula SK12-FO-17
Wood Chips SK12-EN-10
Feed For Fish SK12-FO-18
Industrial Wastewater SK12-EN-11
Fish Oil SK12-FO-19
BCS 300 Soil SK12-EN-12
Fresh Kidney SK12-FO-20
EPA 3051 SK12-EN-13
Fresh Liver SK12-FO-21
Green Algae SK12-EN-14
Lecithin SK12-FO-22
Mud with 25% of organics SK12-EN-15
Lyophilized Meat SK12-FO-23
Raw Waste SK12-EN-16
Margarine SK12-FO-24 Tuna Fish In Oil SK12-FO-47

Milk Powder SK12-FO-25 Vegetable Oil SK12-FO-48

Whole Wheat Grain SK12-FO-26 Wheat Flour SK12-FO-49

Mint Oil SK12-FO-27 Tobacco Leaves SK12-FO-50

Monogliceride SK12-FO-28 Fertilizer SK12-FO-51

Mushroom SK12-FO-29 Lichen SK12-FO-52

Noodles SK12-FO-30 Barley SK12-FO-53

Olives SK12-FO-31 Hop SK12-FO-54

Oyster Tissue SK12-FO-32 Grass SK12-FO-55

Palm Oil SK12-FO-33 Grape Leaves SK12-FO-56

Pet Food SK12-FO-34 Dried Graminacee SK12-FO-57

Raw Sugar SK12-FO-35 Roasted Peanuts/Cereals SK12-FO-58

Reconstituted Inf. FormulaSK12-FO-36 Spinaches SK12-FO-59

Rice Flour SK12-FO-37 Tomatoes SK12-FO-60

Salami SK12-FO-38 Wine SK12-FO-61

Sheep Milk SK12-FO-39 Coffee SK12-FO-62

Soya Fat SK12-FO-40 Butter SK12-FO-63

Soya Oil SK12-FO-41 Fresh Meat SK12-FO-64

Soybean SK12-FO-42 Salad SK12-FO-65

Sunflower seeds SK12-FO-43 Vinegar SK12-FO-66

Tea Leaves SK12-FO-44 Yeast SK12-FO-67

Tinned Beef SK12-FO-45 Corn Flakes SK12-FO-68

Tinned Pork SK12-FO-46

The following application notes are suitable only for the configuration described in each notes
 Application Note SK12-AG-01

Pine Leaves

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten by hands.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-AG-02

Dried Plant Tissue

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-AG-03

Ground Vegetable

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-AG-04

Maize

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-AG-05

Hay

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-AG-06

Beans

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-AG-07

Lentils

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-AG-08

Roots

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
9 ml of HNO3 65%, 1 ml of HF 40%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-AG-09

Tea Leaves

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-01

Acid Slag

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
7 ml of HNO3 65%, 3 ml of HF 40%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-02

Dried Biological Sludge

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-03

Grass

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-EN-04

Leaves

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-05

Municipal Sludge

Summary
This method provides the acid digestion of the
sample in a closed vessel device using 7. Cool the rotor by air or by water until the
temperature control microwave heating for the solution reaches room temperature.
8. Open the vessel and transfer the solution
metal determination by spectroscopic methods.
to a marked flask.
Instrumentation
Microwave program
Microwave acid digestion apparatus (1) (2)
Step Time T1 T2 P Power

Milestone Ethos or Start labstation with internal 1 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with 2 00:15:00 180°C 120°C 30 bar Max power*
easyCONTROL software installed and Pro 24/16 (1), (2) Optional sensors
high throughput rotor. Temperature profile
Sample weight
Up to 0.2 g

Reagents
6 ml of HNO3 65%, 2 ml of H2O2 30%,
2 ml of HF 40%

Procedure
1. Place a TFM vessel on the balance plate, tare
Note
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-EN-06

NIST SRM 2781 Dried Domestic Sludge

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
6 ml of HNO3 65%, 4 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-EN-07

Pollen

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
10 ml of HNO3 65%.

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-08

US EPA Method 3052

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of HF 40%.

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-09

Wastewater

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 2 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-10

Wood chips

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.2 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-11

Industrial Wastewater

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
high throughput rotor.

Sample weight
Up to 1 g

Reagents
6 ml of HNO3 65%, 2 ml of H2O2 30%,

2 ml of HF 40%.

Procedure Note
1. Place a TFM vessel on the balance plate, tare *Max power: 1500W for Ethos and 1200W for Start units.
it and weigh of the sample. Use up to 500 Watt for operations with 5 or less vessels
2. Introduce the TFM vessel into the HTC safety simultaneously.
shield.
This procedure is only a guideline and it may need to be
3. Add the acids; if part of the sample stays on
modified or changed to obtain the required results on your
the inner wall of the TFM vessel, wet it by
sample.
adding acids drop by drop, then gently swirl
the solution to homogenize the sample with Milestone suggest to use pressure sensor (P) only for
the acids. method developments or unknown samples. See the
4. Close the vessel and tighten the cover. pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity Always use hand, eye and body protection when operating
and connect the temperature sensor. with the microwave system.
6. Run the microwave program to completion.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-12

BCS 300 Soil

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.25 g

Reagents
6 ml of HNO3 65%, 4 ml of HF 40%.

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK-EN-13

EPA 3051

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 175°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 175°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
10 ml of HNO3 65%.

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-14

Green Algae

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-15

Mud with 25% Organic

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-16

Raw Waste

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
7 ml of HNO3 65%, 2 ml of H2O2 30%

1 ml of HF 40%.

Procedure Note
1. Place a TFM vessel on the balance plate, tare *Max power: 1500W for Ethos and 1200W for Start units.
it and weigh of the sample. Use up to 500 Watt for operations with 5 or less vessels
2. Introduce the TFM vessel into the HTC safety simultaneously.
shield.
This procedure is only a guideline and it may need to be
3. Add the acids; if part of the sample stays on
modified or changed to obtain the required results on your
the inner wall of the TFM vessel, wet it by
sample.
adding acids drop by drop, then gently swirl
the solution to homogenize the sample with Milestone suggest to use pressure sensor (P) only for
the acids. method developments or unknown samples. See the
4. Close the vessel and tighten the cover. pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity Always use hand, eye and body protection when operating
and connect the temperature sensor. with the microwave system.
6. Run the microwave program to completion.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-EN-17

Siliceus Soil with Organic

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
6 ml of HNO3 65%, 1 ml of H2O2 30%

3 ml of HF 40%.

Procedure Note
1. Place a TFM vessel on the balance plate, tare *Max power: 1500W for Ethos and 1200W for Start units.
it and weigh of the sample. Use up to 500 Watt for operations with 5 or less vessels
2. Introduce the TFM vessel into the HTC safety simultaneously.
shield.
This procedure is only a guideline and it may need to be
3. Add the acids; if part of the sample stays on
modified or changed to obtain the required results on your
the inner wall of the TFM vessel, wet it by
sample.
adding acids drop by drop, then gently swirl
the solution to homogenize the sample with Milestone suggest to use pressure sensor (P) only for
the acids. method developments or unknown samples. See the
4. Close the vessel and tighten the cover. pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity Always use hand, eye and body protection when operating
and connect the temperature sensor. with the microwave system.
6. Run the microwave program to completion.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-EN-18

Urban Wastewater

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of HF 40%.

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-01

Ham

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-02

Agarose

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-03

Baby food

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-04

BCR 100 Beech Leaves

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-05

BCR 185 Fresh Bovine Liver

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.5 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%

Procedure
1. Place a TFM vessel on the balance plate, tare Note
it and weigh of the sample.
*Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety
shield. Use up to 500 Watt for operations with 5 or less vessels
3. Add the acids; if part of the sample stays on simultaneously.
the inner wall of the TFM vessel, wet it by This procedure is only a guideline and it may need to be
adding acids drop by drop, then gently swirl modified or changed to obtain the required results on your
the solution to homogenize the sample with sample.
the acids.
Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover.
method developments or unknown samples. See the
5. Insert the segment into the microwave cavity
pressure sensor user manual for more information.
and connect the temperature sensor.
6. Run the microwave program to completion. Always use hand, eye and body protection when operating
with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-06

BCR 464 Tuna Fish

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
high throughput rotor.

Sample weight
Up to 0.5 g

Reagents
15 ml of HNO3 65%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-07

BCR 60 Aquatic Plant

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
10 ml of HNO3 65%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-08

Bovine Milk

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:15:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-09

Calcium Alginate from SE

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:30:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare *Max power: 1500W for Ethos and 1200W for Start units.
it and weigh of the sample.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
simultaneously.
shield.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with Milestone suggest to use pressure sensor (P) only for
the acids. method developments or unknown samples. See the
4. Close the vessel and tighten the cover. pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
Always use hand, eye and body protection when operating
and connect the temperature sensor.
with the microwave system.
6. Run the microwave program to completion.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-10

Casein

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-11

Cheese Ashes

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.5 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-12

Chewing Gum

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.2 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-13

Chocolate

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-14

Cocoa Powder

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-15

Corn Starch

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-16

Dried Fish

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-17

Dry Infant Formula

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-18

Feed for Fish

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-19

Fish in Oil

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-20

Fresh Kidney

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-21

Fresh Liver

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-22

Lecithin

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011
Application Note SK12-FO-23

Lyophilized Meat

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011
 Application Note SK12-FO-24

Margarine

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.2 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011
 Application Note SK12-FO-25

Milk Powder

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011
 Application Note SK12-FO-26

Whole Wheat Grain

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-27

Mint Oil

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-28

Monogliceride

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.1 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-29

Mushroom

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-30

Noodles

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-31

Olives

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-32

Oyster Tissue

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-33

Palm Oil

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.1 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-34

Pet Food

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-35

Raw Sugar

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note PRO-FO-36

Reconst Infant Formula

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 4 ml

Reagents
8 ml of HNO3 65%, 2ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 04_09-
 Application Note SK12-FO-37

Rice Flour

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature
Temperature profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
6 ml of HNO3 65%, 1 ml of H2O2 30%

3 ml of H2O.
Note
Procedure
*Max power: 1500W for Ethos and 1200W for Start units.
1. Place a TFM vessel on the balance plate, tare Use up to 500 Watt for operations with 5 or less vessels
it and weigh of the sample. simultaneously.
2. Introduce the TFM vessel into the HTC safety
shield. This procedure is only a guideline and it may need to be
3. Add the acids; if part of the sample stays on modified or changed to obtain the required results on your
sample.
the inner wall of the TFM vessel, wet it by
adding acids drop by drop, then gently swirl Milestone suggest to use pressure sensor (P) only for
the solution to homogenize the sample with method developments or unknown samples. See the
the acids. pressure sensor user manual for more information.
4. Close the vessel and tighten the cover. Always use hand, eye and body protection when operating
5. Insert the segment into the microwave cavity with the microwave system.
and connect the temperature sensor.
6. Run the microwave program to completion.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-38

Salami

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-39

Sheep Milk

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-40

Soya Fat

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.1 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-41

Soya Oil

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-42

Soybean

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-43

Sunflower Seeds

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-44

Tea Leaves

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-45

Tinned Beef

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-46

Tinned Pork

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-47

Tuna Fish in Oil

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-48

Vegetable Oil

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-49

Wheat Flour

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011 -
Application Note SK12-FO-50

Tobacco Leaves

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-51

Fertilizer

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-52

Lichen

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:20:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-53

Barley

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on
This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by
modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl
sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
4. Close the vessel and tighten the cover. method developments or unknown samples. See the
5. Insert the segment into the microwave cavity pressure sensor user manual for more information.
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-54

Hop

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-55

Grass

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-56

Grape Leaves

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-57

Dried Graminacee

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-58

Cereals

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-59

Spinache

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-60

Tomatoes

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-61

Wine

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.2 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-62

Coffee

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.2 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-63

Butter

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.2 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-64

Fresh Meat

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
high throughput rotor.

Sample weight
Up to 0.5 g (0.3 g of dry sample max)

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-0/2011-
Application Note SK12-FO-65

Salad

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-66

Vinegar

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
Application Note SK12-FO-67

Yeast

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-
 Application Note SK12-FO-68

Corn Flakes

Summary
This method provides the acid digestion of the 7. Cool the rotor by air or by water until the
sample in a closed vessel device using solution reaches room temperature.
temperature control microwave heating for the 8. Open the vessel and transfer the solution
metal determination by spectroscopic methods. to a marked flask.

Instrumentation Microwave program

(1) (2)
Step Time T1 T2 P Power
Microwave acid digestion apparatus
1 00:20:00 180°C 120°C 30 bar Max power*
Milestone Ethos or Start labstation with internal 2 00:10:00 180°C 120°C 30 bar Max power*
temperature sensor, 640-260 terminal with (1), (2) Optional sensors
easyCONTROL software installed and Pro 24/16
Temperature profile
profile
high throughput rotor.

Sample weight
Up to 0.3 g

Reagents
8 ml of HNO3 65%, 2 ml of H2O2 30%.

Procedure
Note
1. Place a TFM vessel on the balance plate, tare
it and weigh of the sample. *Max power: 1500W for Ethos and 1200W for Start units.
2. Introduce the TFM vessel into the HTC safety Use up to 500 Watt for operations with 5 or less vessels
shield. simultaneously.
3. Add the acids; if part of the sample stays on This procedure is only a guideline and it may need to be
the inner wall of the TFM vessel, wet it by modified or changed to obtain the required results on your
adding acids drop by drop, then gently swirl sample.
the solution to homogenize the sample with
the acids. Milestone suggest to use pressure sensor (P) only for
method developments or unknown samples. See the
4. Close the vessel and tighten the cover.
pressure sensor user manual for more information.
5. Insert the segment into the microwave cavity
and connect the temperature sensor. Always use hand, eye and body protection when operating
6. Run the microwave program to completion. with the microwave system.

Milestone Srl – Helping Chemists www.milestonesrl.com [email protected]

-Rev 0/2011-