AST (IFCC, without Pyridoxal phosphate activation), AU400/AU640

Serum/Plasma Application
System Reagent: OSR6009, OSR6109, OSR6209 Reagent ID: 009

Specific Test Parameters

General LIH ISE Range

Test Name: AST    Type: Serum  Operation: Yes 

Sample: Volume 10 L Dilution 0 L Pre-Dilution Rate: 1

Reagents: R1 Volume 50 L Dilution 50 L Min OD Max OD
R2 Volume 50 L Dilution 100 L L 0.60 H 2.5
Reagent OD limit:
Wavelength: Pri. 340  Sec. 660  First L 1.1 First H 2.5
Method: RATE  Last L 1.1 Last H 2.5
Reaction slope: -  Dynamic Range:
Measuring Point 1: First 14 Last 27 L 3* H 1000*
Measuring Point 2: First Last Correlation Factor:

SERUM/PLASMA APPLICATION
Linearity : 15 % A 1 B 0
No Lag Time: YES  On-board stability period: 30

Specific Test Parameters

General LIH ISE Range

Test Name: AST    Type: Serum 

Value/Flag: #  Level L: # Level H: #

Normal Ranges: Age L Age H
Sex Year Month Year Month L H
 1. #  # # # # # #
 2. #  # # # # # #
 3. #  # # # # # #
 4. #  # # # # # #
 5. #  # # # # # #
 6. #  # # # # # #
7. None Selected # #
8. Out of Range L H # #
Panic Value: # # Unit: U/L* Decimal places: #

Calibration Specific
General ISE

Test Name: AST    Type Serum 

Calibration Type: MB  Formula: Y=AX+B  Counts: # Process: 

Cal. No. OD CONC Factor/OD-L Factor/OD-H

Point 1: § § §3650* §5470*
Point 2:
Point 3:
Point 4:
Point 5:
Point 6:
Point 7:
1-Point Cal. Point:  With CONC-0 Slope Check: None  Advanced Calibration: # 
MB Type Factor: # Calibration Stability Period:

# User defined
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60
§ For use in AB mode only, refer to leaflet for further instruction.
Enzyme BSOSR6x09.07
2018-05
AST (IFCC, without Pyridoxal phosphate activation), AU2700/AU5400 AST (IFCC, without Pyridoxal phosphate activation), AU680/AU480
Serum/Plasma Application
Serum/Plasma Application System Reagent: OSR6009, OSR6109, OSR6209, Reagent ID: 009
System Reagent: OSR6009, OSR6109, OSR6209, OSR6609 Reagent ID: 009 Parameters Specific Test Parameters
General LIH ISE HbA1c Calculated Test Range
Specific Test Parameters
General LIH ISE Range Test Name: AST    Type: Serum  Operation Yes 

Test Name: AST    Type: Serum  Operation: Yes 

Sample Volume 5 L Dilution 0 L OD Limit
Sample: Volume 5 L Dilution 0 L Pre-Dilution Rate: 1 Pre-Dilution Rate 1  Min.OD 0.60 Max.OD 2.5
Reagents: R1 Volume 25 L Dilution 25 L Min OD Max OD Rgt. Volume R1(R1-1) 25 L Dilution 25 L Reagent OD Limit
R2 Volume 25 L Dilution 50 L L 0.60 H 2.5 First Low 1.1 High 2.5
Reagent OD limit: Last Low 1.1 High 2.5
Wavelength: Pri. 340  Sec. 660  First L 1.1 First H 2.5 R2(R2-1) 25 L Dilution 50 L
Method: RATE  Last L 1.1 Last H 2.5 Dynamic Range Low 3* High 1000*
Reaction slope: -  Dynamic Range: Common Rgt. Type Noneф Name ф Correlation Factor A 1 B 0
Measuring Point 1: First 14 Last 27 L 3* H 1000* Wavelength Pri 340 nm Sec. 660 nm Factor for Maker A 1 B 0
Measuring Point 2: First Last Correlation Factor: Method RATE 
Linearity : 15 % A 1 B 0 Reaction Slope -  Onboard Stability Period 30 Day 0 Hour

SERUM/PLASMA APPLICATION
No Lag Time: YES  On-board stability period: 30 Measuring Point1 First 14 Last 27 LIH Influence Check # 
Measuring Point2 First Last Lipemia +++++ 
Linearity Limit 15 % Icterus +++++ 
Lag Time Check YES  Hemolysis + 
Specific Test Parameters
General LIH ISE Range Parameters Specific Test Parameters
General LIH ISE HbA1c Calculated Test Range
Test Name: AST    Type: Serum 
Test Name: AST    Type: Serum 
Value/Flag: #  Level L: # Level H: #
Normal Ranges: Age L Age H Value/Flag: #  Low High
Sex Year Month Year Month L H Level # # Panic Value
 1. #  # # # # # # Specific Ranges: From To Low High
 2. #  # # # # # # Sex Year Month Year Month Low High # #
 3. #  # # # # # #  1. #  # # # # # #
 4. #  # # # # # #  2. #  # # # # # #
 3. #  # # # # # #
 5. #  # # # # # #
 4. #  # # # # # #
 6. #  # # # # # #
 5. #  # # # # # #
7. None Selected # #
 6. #  # # # # # #
8. Out of Range L H # # 7. No demographics # #
Panic Value: # # Unit: U/L* Decimal places: # 8. Not within expected values # #
Unit U/L* Decimal Places #
Parameters Calibration Parameters
Calibration Specific Calibrators Calibration Specific STAT Table Calibration
General ISE General ISE

Test Name: AST    Type Serum  Test Name: AST    Type Serum   Use Serum Cal.

Calibration Type: MB  Formula: Y=AX+B  Counts: # Process:  Calibration Type: MB  Formula: Y=AX+B  Counts: # 
<Calibrator Parameters> Range
Cal. No. OD CONC Factor/OD-L Factor/OD-H Calibrator OD Conc Low High Slope Check None 
Point 1: § § §3650* §5470* Point 1: §  § §3650* §5470*
Point 2: Point 2:  Allowance Range Check
Point 3: Point 3: 
Point 4: Point 4:   Reagent Blank
Point 5: Point 5:   Calibration
Point 6: Point 6: 
Point 7:
Point 7  Advanced Calibration
Point 8  Operation # 
1-Point Cal. Point:  with CONC-0 Slope Check: None  Advanced Calibration: #  Point 9 
MB Type Factor: # Calibration Stability Period: Point 10  Interval (RB/ACAL) # 
<Point Cal. For No. of Correction Points  Use Master Curve   Lot Calibration
Master Curve> OD Range
# User defined Calibrator OD Conc Low High Stability
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60 Point-1  Reagent Blank Day Hour
§ For use in AB mode only. Refer to IFU for further instruction Point-2  Calibration Day Hour
ф AU680
MB Type Factor: # 1-Point Calibration Point   with Conc-0

Enzyme BSOSR6x09.07
2018-05
AST (IFCC, without Pyridoxal phosphate activition) AU5800 Serum/Plasma Application
System Reagent: OSR6009, OSR6109, OSR6209, OSR6609 Reagent ID: 009
Parameters Specific Test Parameters
General LIH ISE HbA1c Calculated Test Range

Test Name: AST    Type: Serum  Operation Yes 

Sample Volume 5 L Dilution 0 L OD Limit

Pre-Dilution Rate 1  Diluent Bottle #  Min.OD 0.60 Max.OD 2.5
Rgt. Volume R1(R1-1) 25 L Dilution 25 L Reagent OD Limit
R1-2 L Dilution L 1st. Low 1.1 High 2.5
Last Low 1.1 High 2.5
R2(R2-1) 25 L Dilution 50 L
Dynamic Range Low 3* High 1000*
Common Rgt. Type None Name None Correlation Factor A 1 B 0
Wavelength Pri 340 nm Sec. 660 nm Factor for Maker A 1 B 0
Method RATE 
Reaction Slope -  Onboard Stability Period 30 Day 0 Hour

SERUM/PLASMA APPLICATION
Measuring Point1 1st 14 Last 27 LIH Influence Check # 
Measuring Point2 1st Last Lipemia +++++ 

SERUM/PLASMA APPLICATION
Linearity Limit 15 % Icterus +++++ 
Lag Time Check YES  Hemolysis + 

Parameters Specific Test Parameters

General LIH ISE HbA1c Calculated Test Range

Test Name: AST    Type: Serum 

Value/Flag: #  .
Level Low # High #
Specific Ranges: From To
Sex Year Month Year Month Low High
 1. #  # # # # # #
 2. #  # # # # # #
 3. #  # # # # # #
 4. #  # # # # # #
 5. #  # # # # # #
 6. #  # # # # # #
7. Standard demographics # #
8. Not within expected values # #
Panic Value Low # High # Unit U/L* Decimal Places #
Parameters Calibration Parameters
Calibrators Calibration Specific
General ISE

Test Name: AST    Type Serum  Cuvette . 

□ Use Serum Cal.

Calibration Type: MB  Formula: Y=AX+B  Counts: # 

<Calibrator Parameters> Range
Calibrator OD Conc Low High Slope Check 
Point 1: §  § §3650* §5470*
Point 2:  Allowance Range Check
Point 3: 
Point 4:  □ Reagent Blank
Point 5:  □ Calibration
Point 6: 
Point 7:  Advanced Calibration
Point 8:  Operation # 
Point 9: 
Point 10:  Interval (RB/ACAL) # 
<Point Cal. For No. of Correction Points  Use Master Curve  □ Lot Calibration
Master Curve> OD Range
Calibrator OD Conc Low High Stability
Point-1  Reagent Blank Day Hour # User defined
Point-2  Calibration Day Hour * Values set for working in U/L. To work in SI units (µkat/L) divide by 60
MB Type Factor: # 1-Point Calibration Point  □ with Conc-0 § For use in AB mode only, refer to IFU for further instruction

Enzyme BSOSR6x09.07
2018-05
AST (IFCC, with OE60106 activation), AU400/AU640 Serum/Plasma
Application
System Reagent: OSR6009, OSR6109, OSR6209 Reagent ID: 009

Specific Test Parameters

General LIH ISE Range

Test Name: AST    Type: Serum  Operation: Yes 

Sample: Volume 10 L Dilution 0 L Pre-Dilution Rate: 1

Reagents: R1 Volume 50 L Dilution 50 L Min OD Max OD
R2 Volume 50 L Dilution 100 L L 1.0 H 2.5
Reagent OD limit:
Wavelength: Pri. 340  Sec. 660  First L 1.3 First H 2.5
Method: RATE  Last L 1.3 Last H 2.5
Reaction slope: -  Dynamic Range:
Measuring Point 1: First 14 Last 27 L 3* H 1000*
Measuring Point 2: First Last Correlation Factor:
Linearity : 15 % A 1 B 0

SERUM/PLASMA APPLICATION
No Lag Time: YES  On-board stability period: 7

Specific Test Parameters

General LIH ISE Range

Test Name: AST    Type: Serum 

Value/Flag: #  Level L: # Level H: #

Normal Ranges: Age L Age H
Sex Year Month Year Month L H
 1. #  # # # # # #
 2. #  # # # # # #
 3. #  # # # # # #
 4. #  # # # # # #
 5. #  # # # # # #
 6. #  # # # # # #
7. None Selected # #
8. Out of Range L H # #
Panic Value: # # Unit: U/L* Decimal places: #

Calibration Specific
General ISE

Test Name: AST    Type Serum 

Calibration Type: MB  Formula: Y=AX+B  Counts: # Process: 

Cal. No. OD CONC Factor/OD-L Factor/OD-H

Point 1: § § §3650* §5470*
Point 2:
Point 3:
Point 4:
Point 5:
Point 6:
Point 7:
1-Point Cal. Point:  with CONC-0 Slope Check: None  Advanced Calibration: # 
MB Type Factor: # Calibration Stability Period:

# User defined
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60
§ For use in AB mode only, refer to IFU for further instruction.

Enzyme BSOSR6x09.07
2018-05
AST (IFCC, with OE60106 activation), AU2700/AU5400 Serum/Plasma AST (IFCC, with OE60106 activition) AU5800 Serum/Plasma Application
System Reagent: OSR6009, OSR6109, OSR6209, OSR6609 Reagent ID: 009
Application Parameters Specific Test Parameters
System Reagent: OSR6009, OSR6109, OSR6209, OSR6609 Reagent ID: 009 General LIH ISE HbA1c Calculated Test Range

Test Name: AST    Type: Serum  Operation Yes 

Specific Test Parameters
General LIH ISE Range
Sample Volume 5 L Dilution 0 L OD Limit
Test Name: AST    Type: Serum  Operation: Yes  Pre-Dilution Rate 1  Diluent Bottle #  Min.OD 1.0 Max.OD 2.5
Sample: Volume 5 L Dilution 0 L Pre-Dilution Rate: 1 Rgt. Volume R1(R1-1) 25 L Dilution 25 L Reagent OD Limit
Reagents: R1 Volume 25 L Dilution 25 L Min OD Max OD R1-2 L Dilution L 1st. Low 1.3 High 2.5
Last Low 1.3 High 2.5
R2 Volume 25 L Dilution 50 L L 1.0 H 2.5
R2(R2-1) 25 L Dilution 50 L
Reagent OD limit:
Dynamic Range Low 3* High 1000*
Wavelength: Pri. 340  Sec. 660  First L 1.3 First H 2.5
Common Rgt. Type None Name None Correlation Factor A 1 B 0
Method: RATE  Last L 1.3 Last H 2.5
Wavelength Pri 340 nm Sec. 660 nm Factor for Maker A 1 B 0
Reaction slope: -  Dynamic Range:
Method RATE 
Measuring Point 1: First 14 Last 27 L 3* H 1000* Reaction Slope -  Onboard Stability Period 7 Day 0 Hour
Measuring Point 2: First Last Correlation Factor: Measuring Point1 1st 14 Last 27 LIH Influence Check # 
Linearity : 15 % A 1 B 0

SERUM/PLASMA APPLICATION
Measuring Point2 1st Last Lipemia +++++ 
No Lag Time: YES  On-board stability period: 7 Linearity Limit 15 % Icterus +++++ 
Lag Time Check YES  Hemolysis + 

Specific Test Parameters Parameters Specific Test Parameters

General LIH ISE Range General LIH ISE HbA1c Calculated Test Range

Test Name: AST    Type: Serum  Test Name: AST    Type: Serum 

Value/Flag: #  Level L: # Level H: # Value/Flag: # 

Normal Ranges: Age L Age H Level Low # High #
Sex Year Month Year Month L H Specific Ranges: From To
Sex Year Month Year Month Low High
 1. #  # # # # # #
 1. #  # # # # # #
 2. #  # # # # # #
 2. #  # # # # # #
 3. #  # # # # # #  3. #  # # # # # #
 4. #  # # # # # #  4. #  # # # # # #
 5. #  # # # # # #  5. #  # # # # # #
 6. #  # # # # # #  6. #  # # # # # #
7. None Selected # # 7. Standard demographics # #
8. Out of Range L H # # 8. Not within expected values # #
Panic Value: # # Unit: U/L* Decimal places: # Panic Value Low # High # Unit U/L* Decimal Places #
Parameters Calibration Parameters
Calibrators Calibration Specific
General ISE
Calibration Specific
General ISE Test Name: AST    Type Serum  Cuvette . 
□ Use Serum Cal.
Test Name: AST    Type Serum 
Calibration Type: MB  Formula: Y=AX+B  Counts: # 
Calibration Type: MB  Formula: Y=AX+B  Counts: # Process:  <Calibrator Parameters> Range
Calibrator OD Conc Low High Slope Check 
Cal. No. OD CONC Factor/OD-L Factor/OD-H Point 1: §  § §3650* §5470*
Point 1: § § §3650* §5470* Point 2:  Allowance Range Check
Point 2: Point 3: 
Point 3: Point 4:  □ Reagent Blank
Point 4: Point 5:  □ Calibration
Point 5: Point 6: 
Point 6: Point 7:  Advanced Calibration
Point 7: Point 8:  Operation # 
1-Point Cal. Point:  with CONC-0 Slope Check: None  Advanced Calibration: #  Point 9: 
Point 10:  Interval (RB/ACAL) # 
MB Type Factor: # Calibration Stability Period:
<Point Cal. For No. of Correction Points  Use Master Curve  □ Lot Calibration
Master Curve> OD Range
Calibrator OD Conc Low High Stability
# User defined Point-1  Reagent Blank Day Hour
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60 Point-2  Calibration Day Hour
§ For use in AB mode only. Refer to IFU for further instruction
MB Type Factor: # 1-Point Calibration Point  □ with Conc-0

Enzyme BSOSR6x09.07
2018-05
AST (IFCC, with OE60106 activation ), AU680/AU480 AST (IFCC, with OSR60180 activation), AU680
Serum/Plasma Application Serum/Plasma Application
System Reagent: OSR6009, OSR6109, OSR6209 Reagent ID: 009 System Reagent: OSR6009, OSR6109, OSR6209 Reagent ID: 009
Parameters Specific Test Parameters Parameters Specific Test Parameters
General LIH ISE HbA1c Calculated Test Range General LIH ISE HbA1c Calculated Test Range

Test Name: AST    Type: Serum  Operation Yes  Test Name: AST    Type: Serum  Operation Yes 

Sample Volume 5 L Dilution 0 L OD Limit Sample Volume 5 L Dilution 0 L OD Limit

Pre-Dilution Rate 1  Min.OD 1.0 Max.OD 2.5 Pre-Dilution Rate 1  Min.OD 1.0 Max.OD 2.5
Rgt. Volume R1(R1-1) 25 L Dilution 25 L Reagent OD Limit Rgt. Volume R1(R1-1) 25 L Dilution 10 L Reagent OD Limit
First Low 1.3 High 2.5 R1-2 5 L Dilution 10 L First Low 1.3 High 2.5
Last Low 1.3 High 2.5 Last Low 1.3 High 2.5
R2(R2-1) 25 L Dilution 50 L R2(R2-1) 25 L Dilution 50 L
Dynamic Range Low 3* High 1000* Dynamic Range Low 3* High 1000*
Common Rgt. Type Noneф Name ф Correlation Factor A 1 B 0 Common Rgt. Type R1-2 Name # Correlation Factor A 1 B 0
Wavelength Pri 340 nm Sec. 660 nm Factor for Maker A 1 B 0 Wavelength Pri 340 nm Sec. 660 nm Factor for Maker A 1 B 0
Method RATE  Method RATE 
Reaction Slope -  Onboard Stability Period 7 Day 0 Hour Reaction Slope -  Onboard Stability Period 30 Day 0 Hour
Measuring Point1 First 14 Last 27 LIH Influence Check #  Measuring Point1 First 14 Last 27 LIH Influence Check # 
Measuring Point2 First Last Lipemia +++++  Measuring Point2 First Last Lipemia +++++ 

SERUM/PLASMA APPLICATION
Linearity Limit 15 % Icterus +++++  Linearity Limit 15 % Icterus +++++ 
Lag Time Check YES  Hemolysis +  Lag Time Check YES  Hemolysis + 

Parameters Specific Test Parameters Parameters Specific Test Parameters

General LIH ISE HbA1c Calculated Test Range General LIH ISE HbA1c Calculated Test Range

Test Name: AST    Type: Serum  Test Name: AST    Type: Serum 

Value/Flag: #  Low High Value/Flag: #  Low High

Level # # Panic Value Level # # Panic Value
Specific Ranges: From To Low High Specific Ranges: From To Low High
Sex Year Month Year Month Low High # # Sex Year Month Year Month Low High # #
 1. #  # # # # # #  1. #  # # # # # #
 2. #  # # # # # #  2. #  # # # # # #
 3. #  # # # # # #  3. #  # # # # # #
 4. #  # # # # # #  4. #  # # # # # #
 5. #  # # # # # #  5. #  # # # # # #
 6. #  # # # # # #  6. #  # # # # # #
7. No demographics # # 7. No demographics # #
8. Not within expected values # # 8. Not within expected values # #
Unit U/L* Decimal Places # Unit U/L* Decimal Places #
Parameters Calibration Parameters Parameters Calibration Parameters
Calibrators Calibration Specific STAT Table Calibration Calibrators Calibration Specific STAT Table Calibration
General ISE General ISE

Test Name: AST    Type Serum   Use Serum Cal. Test Name: AST    Type Serum   Use Serum Cal.

Calibration Type: MB  Formula: Y=AX+B  Counts: #  Calibration Type: MB  Formula: Y=AX+B  Counts: # 
<Calibrator Parameters> Range <Calibrator Parameters> Range
Calibrator OD Conc Low High Slope Check None  Calibrator OD Conc Low High Slope Check None 
Point 1: §  § §3650* §5470* Point 1: §  § §3650* §5470*
Point 2:  Allowance Range Check Point 2:  Allowance Range Check
Point 3:  Point 3: 
Point 4:   Reagent Blank Point 4:   Reagent Blank
Point 5:   Calibration Point 5:   Calibration
Point 6:  Point 6: 
Point 7  Advanced Calibration Point 7  Advanced Calibration
Point 8  Operation #  Point 8  Operation # 
Point 9  Point 9 
Point 10  Interval (RB/ACAL) #  Point 10  Interval (RB/ACAL) # 
<Point Cal. For No. of Correction Points  Use Master Curve   Lot Calibration <Point Cal. For No. of Correction Points  Use Master Curve   Lot Calibration
Master Curve> OD Range Master Curve> OD Range
Calibrator OD Conc Low High Stability Calibrator OD Conc Low High Stability
Point-1  Reagent Blank Day Hour Point-1  Reagent Blank Day Hour
Point-2  Calibration Day Hour Point-2  Calibration Day Hour
MB Type Factor: # 1-Point Calibration Point   with Conc-0 MB Type Factor: # 1-Point Calibration Point   with Conc-0

Enzyme BSOSR6x09.07
2018-05
AST (IFCC, with OSR60180 activition) AU5800 Serum/Plasma Application
System Reagent: OSR6009, OSR6109, OSR6209, OSR6609 Reagent ID: 009
Parameters Specific Test Parameters
General LIH ISE HbA1c Calculated Test Range

Test Name: AST    Type: Serum  Operation Yes 

Sample Volume 5 L Dilution 0 L OD Limit

Pre-Dilution Rate 1  Diluent Bottle #  Min.OD 1.0 Max.OD 2.5
Rgt. Volume R1(R1-1) 25 L Dilution 10 L Reagent OD Limit
R1-2 5 L Dilution 10 L 1st. Low 1.3 High 2.5
Last Low 1.3 High 2.5
R2(R2-1) 25 L Dilution 50 L
Dynamic Range Low 3* High 1000*
Common Rgt. Type R1-2 Name # Correlation Factor A 1 B 0
Wavelength Pri 340 nm Sec. 660 nm Factor for Maker A 1 B 0
Method RATE 

SERUM/PLASMA APPLICATION
Reaction Slope -  Onboard Stability Period 30 Day 0 Hour
Measuring Point1 1st 14 Last 27 LIH Influence Check # 
Measuring Point2 1st Last Lipemia +++++ 
Linearity Limit 15 % Icterus +++++ 
Lag Time Check YES  Hemolysis + 

Parameters Specific Test Parameters

General LIH ISE HbA1c Calculated Test Range

Test Name: AST    Type: Serum 

Value/Flag: # 
Level Low # High #
Specific Ranges: From To
Sex Year Month Year Month Low High
 1. #  # # # # # #
 2. #  # # # # # #
 3. #  # # # # # #
 4. #  # # # # # #
 5. #  # # # # # #
 6. #  # # # # # #
7. Standard demographics # #
8. Not within expected values # #
Panic Value Low # High # Unit U/L* Decimal Places #
Parameters Calibration Parameters
Calibrators Calibration Specific
General ISE

Test Name: AST    Type Serum  Cuvette . 

□ Use Serum Cal.

Calibration Type: MB  Formula: Y=AX+B  Counts: # 

<Calibrator Parameters> Range
Calibrator OD Conc Low High Slope Check None 
Point 1: §  § §3650* §5470*
Point 2:  Allowance Range Check
Point 3: 
Point 4:  □ Reagent Blank
Point 5:  □ Calibration
Point 6: 
Point 7:  Advanced Calibration
Point 8:  Operation # 
Point 9: 
Point 10:  Interval (RB/ACAL) # 
<Point Cal. For No. of Correction Points  Use Master Curve  □ Lot Calibration
Master Curve> OD Range
Calibrator OD Conc Low High Stability
Point-1  Reagent Blank Day Hour
Point-2  Calibration Day Hour
# User defined
MB Type Factor: # 1-Point Calibration Point  □ with Conc-0
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60
§ For use in AB mode only. Refer to IFU for further instruction

Enzyme BSOSR6x09.07
2018-05
AST (IFCC, without Pyridoxal phosphate activation), AU400/AU640
Paediatric Application
System Reagent: OSR6009, OSR6109, OSR6209 Reagent ID: 009
Specific Test Parameters
General LIH ISE Range

Test Name: ASTP    Type: Serum  Operation: Yes 

Sample: Volume 10 L Dilution 10 L Pre-Dilution Rate: 1

Reagents: R1 Volume 50 L Dilution 50 L Min OD Max OD
R2 Volume 50 L Dilution 90 L L 0.60 H 2.5
Reagent OD limit:
Wavelength: Pri. 340  Sec. 660  First L 1.1 First H 2.5
Method: RATE  Last L 1.1 Last H 2.5
Reaction slope: -  Dynamic Range:
Measuring Point 1: First 14 Last 27 L 3* H 1000*
Measuring Point 2: First Last Correlation Factor:
Linearity : 15 % A 1 B 0
No Lag Time: YES  On-board stability period: 30

PAEDIATRIC APPLICATION
Specific Test Parameters
General LIH ISE Range

Test Name: ASTP    Type: Serum 

Value/Flag: #  Level L: # Level H: #

Normal Ranges: Age L Age H
Sex Year Month Year Month L H
 1. #  # # # # # #
 2. #  # # # # # #
 3. #  # # # # # #
 4. #  # # # # # #
 5. #  # # # # # #
 6. #  # # # # # #
7. None Selected # #
8. Out of Range L H # #
Panic Value: # # Unit: U/L* Decimal places: #

Calibration Specific
General ISE

Test Name: ASTP    Type Serum 

Calibration Type: MB  Formula: Y=AX+B  Counts: # Process: 

Cal. No. OD CONC Factor/OD-L Factor/OD-H

Point 1: § § §3650* §5470*
Point 2:
Point 3:
Point 4:
Point 5:
Point 6:
Point 7:
1-Point Cal. Point:  with CONC-0 Slope Check: None  Advanced Calibration: # 
MB Type Factor: # Calibration Stability Period:

# User defined
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60
§ For use in AB mode only, refer to IFU for further instruction.

Enzyme BSOSR6x09.07
2018-05
AST (IFCC, without Pyridoxal phosphate activation), AU2700/AU5400 AST (IFCC, without Pyridoxal phosphate activation), AU680/AU480
Paediatric Application
Paediatric Application System Reagent: OSR6009, OSR6109, OSR6209 Reagent ID: 009
System Reagent: OSR6009, OSR6109, OSR6209, OSR6609 Reagent ID: 009 Parameters Specific Test Parameters
Specific Test Parameters General LIH ISE HbA1c Calculated Test Range
General LIH ISE Range Test Name: ASTP    Type: Serum  Operation Yes 
Test Name: ASTP    Type: Serum  Operation: Yes 
Sample Volume 5 L Dilution 10 L OD Limit
Sample: Volume 5 L Dilution 10 L Pre-Dilution Rate: 1
Pre-Dilution Rate 1  Min.OD 0.60 Max.OD 2.5
Reagents: R1 Volume 25 L Dilution 25 L Min OD Max OD
Rgt. Volume R1(R1-1) 25 L Dilution 25 L Reagent OD Limit
R2 Volume 25 L Dilution 40 L L 0.60 H 2.5
First Low 1.1 High 2.5
Reagent OD limit: Last Low 1.1 High 2.5
Wavelength: Pri. 340  Sec. 660  First L 1.1 First H 2.5 R2(R2-1) 25 L Dilution 40 L
Method: RATE  Last L 1.1 Last H 2.5 Dynamic Range Low 3* High 1000*
Reaction slope: -  Dynamic Range: Common Rgt. Type Noneф Name ф Correlation Factor A 1 B 0
Measuring Point 1: First 14 Last 27 L 3* H 1000* Wavelength Pri 340 nm Sec. 660 nm Factor for Maker A 1 B 0
Measuring Point 2: First Last Correlation Factor: Method RATE 
Linearity : 15 % A 1 B 0 Reaction Slope -  Onboard Stability Period 30 Day 0 Hour
No Lag Time: YES  On-board stability period: 30 Measuring Point1 First 14 Last 27 LIH Influence Check # 

PAEDIATRIC APPLICATION
Measuring Point2 First Last Lipemia +++++ 
Linearity Limit 15 % Icterus +++++ 
Specific Test Parameters Lag Time Check YES  Hemolysis + 
General LIH ISE Range
Parameters Specific Test Parameters
Test Name: ASTP    Type: Serum  General LIH ISE HbA1c Calculated Test Range

Value/Flag: #  Level L: # Level H: # Test Name: ASTP    Type: Serum 

Normal Ranges: Age L Age H
Value/Flag: #  Low High
Sex Year Month Year Month L H
Level # # Panic Value
 1. #  # # # # # #
Specific Ranges: From To Low High
 2. #  # # # # # # Sex Year Month Year Month Low High # #
 3. #  # # # # # #  1. #  # # # # # #
 4. #  # # # # # #  2. #  # # # # # #
 5. #  # # # # # #  3. #  # # # # # #
 6. #  # # # # # #  4. #  # # # # # #
7. None Selected # #  5. #  # # # # # #
8. Out of Range L H # #  6. #  # # # # # #
7. No demographics # #
Panic Value: # # Unit: U/L* Decimal places: #
8. Not within expected values # #
Unit U/L* Decimal Places #
Parameters Calibration Parameters
Calibration Specific
Calibrators Calibration Specific STAT Table Calibration
General ISE General ISE
Test Name: ASTP    Type Serum  Test Name: ASTP    Type Serum   Use Serum Cal.

Calibration Type: MB  Formula: Y=AX+B  Counts: # Process:  Calibration Type: MB  Formula: Y=AX+B  Counts: # 
<Calibrator Parameters> Range
Cal. No. OD CONC Factor/OD-L Factor/OD-H Calibrator OD Conc Low High Slope Check None 
Point 1: § § §3650* §5470* Point 1: §  § §3650* §5470*
Point 2: Point 2:  Allowance Range Check
Point 3: Point 3: 
Point 4: Point 4:   Reagent Blank
Point 5: Point 5:   Calibration
Point 6: Point 6: 
Point 7: Point 7  Advanced Calibration
1-Point Cal. Point:  with CONC-0 Slope Check: None  Advanced Calibration: #  Point 8  Operation # 
Point 9 
MB Type Factor: # Calibration Stability Period:
Point 10  Interval (RB/ACAL) # 
<Point Cal. For No. of Correction Points  Use Master Curve   Lot Calibration
Master Curve> OD Range
# User defined
Calibrator OD Conc Low High Stability
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60
Point-1  Reagent Blank Day Hour
§ For use in AB mode only. Refer to IFU for further instruction
ф AU680 Point-2  Calibration Day Hour
MB Type Factor: # 1-Point Calibration Point   with Conc-0

Enzyme BSOSR6x09.07
2018-05
AST (IFCC, without Pyridoxal phosphate activition) AU5800 Paediatric Application
System Reagent: OSR6009, OSR6109, OSR6209, OSR6609 Reagent ID: 009
Parameters Specific Test Parameters
General LIH ISE HbA1c Calculated Test Range

Test Name: ASTP    Type: Serum  Operation Yes 

Sample Volume 5 L Dilution 10 L OD Limit

Pre-Dilution Rate 1  Diluent Bottle #  Min.OD 0.60 Max.OD 2.5
Rgt. Volume R1(R1-1) 25 L Dilution 25 L Reagent OD Limit
R1-2 L Dilution L 1st. Low 1.1 High 2.5
Last Low 1.1 High 2.5
R2(R2-1) 25 L Dilution 40 L
Dynamic Range Low 3* High 1000*
Common Rgt. Type None Name None Correlation Factor A 1 B 0
Wavelength Pri 340 nm Sec. 660 nm Factor for Maker A 1 B 0
Method RATE 
Reaction Slope -  Onboard Stability Period 30 Day 0 Hour

SERUM/PLASMA APPLICATION
Measuring Point1 1st 14 Last 27 LIH Influence Check # 
Measuring Point2 1st Last Lipemia +++++ 
Linearity Limit 15 % Icterus +++++ 
Lag Time Check YES  Hemolysis + 

PAEDIATRIC APPLICATION
Parameters Specific Test Parameters
General LIH ISE HbA1c Calculated Test Range

Test Name: ASTP    Type: Serum 

Value/Flag: #  .
Level Low # High #
Specific Ranges: From To
Sex Year Month Year Month Low High
 1. #  # # # # # #
 2. #  # # # # # #
 3. #  # # # # # #
 4. #  # # # # # #
 5. #  # # # # # #
 6. #  # # # # # #
7. Standard demographics # #
8. Not within expected values # #
Panic Value Low # High # Unit U/L* Decimal Places #
Parameters Calibration Parameters
Calibrators Calibration Specific
General ISE

Test Name: ASTP    Type Serum  Cuvette . 

□ Use Serum Cal.

Calibration Type: MB  Formula: Y=AX+B  Counts: # 

<Calibrator Parameters> Range
Calibrator OD Conc Low High Slope Check 
Point 1: §  § §3650* §5470*
Point 2:  Allowance Range Check
Point 3: 
Point 4:  □ Reagent Blank
Point 5:  □ Calibration
Point 6: 
Point 7:  Advanced Calibration
Point 8:  Operation # 
Point 9: 
Point 10:  Interval (RB/ACAL) # 
<Point Cal. For No. of Correction Points  Use Master Curve  □ Lot Calibration
Master Curve> OD Range
Calibrator OD Conc Low High Stability
Point-1  Reagent Blank Day Hour # User defined
Point-2  Calibration Day Hour * Values set for working in U/L. To work in SI units (µkat/L) divide by 60
MB Type Factor: # 1-Point Calibration Point  □ with Conc-0 § For use in AB mode only, refer to IFU for further instruction

Enzyme BSOSR6x09.07
2018-05
AST (IFCC, with OE60106 activation), AU400/AU640
Paediatric Application
System Reagent: OSR6009, OSR6109, OSR6209 Reagent ID: 009

Specific Test Parameters

General LIH ISE Range

Test Name: ASTP    Type: Serum  Operation: Yes 

Sample: Volume 10 L Dilution 10 L Pre-Dilution Rate: 1

Reagents: R1 Volume 50 L Dilution 50 L Min OD Max OD
R2 Volume 50 L Dilution 90 L L 1.0 H 2.5
Reagent OD limit:
Wavelength: Pri. 340  Sec. 660  First L 1.3 First H 2.5
Method: RATE  Last L 1.3 Last H 2.5
Reaction slope: -  Dynamic Range:
Measuring Point 1: First 14 Last 27 L 3* H 1000*
Measuring Point 2: First Last Correlation Factor:
Linearity : 15 % A 1 B 0
No Lag Time: YES  On-board stability period: 7

PAEDIATRIC APPLICATION
Specific Test Parameters
General LIH ISE Range

Test Name: ASTP    Type: Serum 

Value/Flag: #  Level L: # Level H: #

Normal Ranges: Age L Age H
Sex Year Month Year Month L H
 1. #  # # # # # #
 2. #  # # # # # #
 3. #  # # # # # #
 4. #  # # # # # #
 5. #  # # # # # #
 6. #  # # # # # #
7. None Selected # #
8. Out of Range L H # #
Panic Value: # # Unit: U/L* Decimal places: #

Calibration Specific
General ISE

Test Name: ASTP    Type Serum 

Calibration Type: MB  Formula: Y=AX+B  Counts: # Process: 

Cal. No. OD CONC Factor/OD-L Factor/OD-H

Point 1: § § §3650* §5470*
Point 2:
Point 3:
Point 4:
Point 5:
Point 6:
Point 7:
1-Point Cal. Point:  with CONC-0 Slope Check: None  Advanced Calibration: # 
MB Type Factor: # Calibration Stability Period:

# User defined
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60
§ For use in AB mode only, refer to IFU for further instruction.
Enzyme BSOSR6x09.07
2018-05
AST (IFCC, with OE60106 activation), AU2700/AU5400 AST (IFCC, with OE60106 activition) AU5800 Paediatric Application
System Reagent: OSR6009, OSR6109, OSR6209, OSR6609 Reagent ID: 009
Paediatric Application Parameters Specific Test Parameters
System Reagent: OSR6009, OSR6109, OSR6209, OSR6609 Reagent ID: 009 General LIH ISE HbA1c Calculated Test Range

Specific Test Parameters Test Name: ASTP    Type: Serum  Operation Yes 
General LIH ISE Range

Test Name: ASTP    Type: Serum  Operation: Yes  Sample Volume 5 L Dilution 10 L OD Limit
Pre-Dilution Rate 1  Diluent Bottle #  Min.OD 1.0 Max.OD 2.5
Sample: Volume 5 L Dilution 10 L Pre-Dilution Rate: 1 Rgt. Volume R1(R1-1) 25 L Dilution 25 L Reagent OD Limit
Reagents: R1 Volume 25 L Dilution 25 L Min OD Max OD R1-2 L Dilution L 1st. Low 1.3 High 2.5
R2 Volume 25 L Dilution 40 L L 1.0 H 2.5 Last Low 1.3 High 2.5
Reagent OD limit: R2(R2-1) 25 L Dilution 40 L
Wavelength: Pri. 340  Sec. 660  First L 1.3 First H 2.5 Dynamic Range Low 3* High 1000*
Method: RATE  Last L 1.3 Last H 2.5 Common Rgt. Type None Name None Correlation Factor A 1 B 0
Reaction slope: -  Dynamic Range: Wavelength Pri 340 nm Sec. 660 nm Factor for Maker A 1 B 0
Measuring Point 1: First 14 Last 27 L 3* H 1000* Method RATE 
Measuring Point 2: First Last Correlation Factor: Reaction Slope -  Onboard Stability Period 7 Day 0 Hour
Linearity : 15 % A 1 B 0 Measuring Point1 1st 14 Last 27 LIH Influence Check # 
Measuring Point2 1st Last Lipemia +++++ 
No Lag Time: YES  On-board stability period: 7
Linearity Limit 15 % Icterus +++++ 

PAEDIATRIC APPLICATION
Lag Time Check YES  Hemolysis + 

Specific Test Parameters Parameters Specific Test Parameters

General LIH ISE Range General LIH ISE HbA1c Calculated Test Range

Test Name: ASTP    Type: Serum  Test Name: ASTP    Type: Serum 

Value/Flag: #  Level L: # Level H: # Value/Flag: # 

Normal Ranges: Age L Age H Level Low # High #
Sex Year Month Year Month L H Specific Ranges: From To
 1. #  # # # # # # Sex Year Month Year Month Low High
 2. #  # # # # # #  1. #  # # # # # #
 2. #  # # # # # #
 3. #  # # # # # #
 3. #  # # # # # #
 4. #  # # # # # #
 4. #  # # # # # #
 5. #  # # # # # #
 5. #  # # # # # #
 6. #  # # # # # #  6. #  # # # # # #
7. None Selected # # 7. Standard demographics # #
8. Out of Range L H # # 8. Not within expected values # #
Panic Value: # # Unit: U/L* Decimal places: # Panic Value Low # High # Unit U/L* Decimal Places #
Parameters Calibration Parameters
Calibrators Calibration Specific
Calibration Specific General ISE
General ISE Test Name: ASTP    Type Serum  Cuvette . 
□ Use Serum Cal.
Test Name: ASTP    Type Serum 
Calibration Type: MB  Formula: Y=AX+B  Counts: # 
Calibration Type: MB  Formula: Y=AX+B  Counts: # Process:  <Calibrator Parameters> Range
Calibrator OD Conc Low High Slope Check 
Cal. No. OD CONC Factor/OD-L Factor/OD-H
Point 1: §  § §3650* §5470*
Point 1: § § §3650* §5470*
Point 2:  Allowance Range Check
Point 2: Point 3: 
Point 3: Point 4:  □ Reagent Blank
Point 4: Point 5:  □ Calibration
Point 5: Point 6: 
Point 6: Point 7:  Advanced Calibration
Point 7: Point 8:  Operation # 
1-Point Cal. Point:  with CONC-0 Slope Check: None Advanced Calibration: #  Point 9: 
MB Type Factor: # Calibration Stability Period: Point 10:  Interval (RB/ACAL) # 
<Point Cal. For No. of Correction Points  Use Master Curve  □ Lot Calibration
Master Curve> OD Range
# User defined Calibrator OD Conc Low High Stability
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60 Point-1  Reagent Blank Day Hour
§ For use in AB mode only. Refer to IFU for further instruction Point-2  Calibration Day Hour
MB Type Factor: # 1-Point Calibration Point  □ with Conc-0

Enzyme BSOSR6x09.07
2018-05
AST (IFCC, with OE60106 activation), AU680/AU480 Paediatric Application AST (IFCC, with OSR60180 activation), AU680 Paediatric Application
System Reagent: OSR6009, OSR6109, OSR6209, Reagent ID: 009 System Reagent: OSR6009, OSR6109, OSR6209 Reagent ID: 009
Parameters Specific Test Parameters Parameters Specific Test Parameters
General LIH ISE HbA1c Calculated Test Range General LIH ISE HbA1c Calculated Test Range

Test Name: ASTP    Type: Serum  Operation Yes  Test Name: ASTP    Type: Serum  Operation Yes 

Sample Volume 5 L Dilution 10 L OD Limit Sample Volume 5 L Dilution 10 L OD Limit

Pre-Dilution Rate 1  Min.OD 1.0 Max.OD 2.5 Pre-Dilution Rate 1  Min.OD 1.0 Max.OD 2.5
Rgt. Volume R1(R1-1) 25 L Dilution 25 L Reagent OD Limit Rgt. Volume R1(R1-1) 25 L Dilution 10 L Reagent OD Limit
First Low 1.3 High 2.5 R1-2 5 L Dilution 10 L First Low 1.3 High 2.5
Last Low 1.3 High 2.5 Last Low 1.3 High 2.5
R2(R2-1) 25 L Dilution 40 L R2(R2-1) 25 L Dilution 40 L
Dynamic Range Low 3* High 1000* Dynamic Range Low 3* High 1000*
Common Rgt. Type Noneф Name ф Correlation Factor A 1 B 0 Common Rgt. Type R1-2 Name # Correlation Factor A 1 B 0
Wavelength Pri 340 nm Sec. 660 nm Factor for Maker A 1 B 0 Wavelength Pri 340 nm Sec. 660 nm Factor for Maker A 1 B 0
Method RATE  Method RATE 
Reaction Slope -  Onboard Stability Period 7 Day 0 Hour Reaction Slope -  Onboard Stability Period 30 Day 0 Hour
Measuring Point1 First 14 Last 27 LIH Influence Check #  Measuring Point1 First 14 Last 27 LIH Influence Check # 
Measuring Point2 First Last Lipemia +++++  Measuring Point2 First Last Lipemia +++++ 
Linearity Limit 15 % Icterus +++++  Linearity Limit 15 % Icterus +++++ 
Lag Time Check YES  Hemolysis +  Lag Time Check YES  Hemolysis + 

PAEDIATRIC APPLICATION
Parameters Specific Test Parameters Parameters Specific Test Parameters
General LIH ISE HbA1c Calculated Test Range General LIH ISE HbA1c Calculated Test Range

Test Name: ASTP    Type: Serum  Test Name: ASTP    Type: Serum 

Value/Flag: #  Low High Value/Flag: #  Low High

Level # # Panic Value Level # # Panic Value
Specific Ranges: From To Low High Specific Ranges: From To Low High
Sex Year Month Year Month Low High # # Sex Year Month Year Month Low High # #
 1. #  # # # # # #  1. #  # # # # # #
 2. #  # # # # # #  2. #  # # # # # #
 3. #  # # # # # #  3. #  # # # # # #
 4. #  # # # # # #  4. #  # # # # # #
 5. #  # # # # # #  5. #  # # # # # #
 6. #  # # # # # #  6. #  # # # # # #
7. No demographics # # 7. No demographics # #
8. Not within expected values # # 8. Not within expected values # #
Unit U/L* Decimal Places # Unit U/L* Decimal Places #
Parameters Calibration Parameters Parameters Calibration Parameters
Calibrators Calibration Specific STAT Table Calibration Calibrators Calibration Specific STAT Table Calibration
General ISE General ISE

Test Name: ASTP    Type Serum   Use Serum Cal. Test Name: ASTP    Type Serum   Use Serum Cal.

Calibration Type: MB  Formula: Y=AX+B  Counts: #  Calibration Type: MB  Formula: Y=AX+B  Counts: # 
<Calibrator Parameters> Range <Calibrator Parameters> Range
Calibrator OD Conc Low High Slope Check None  Calibrator OD Conc Low High Slope Check None 
Point 1: §  § §3650* §5470* Point 1: §  § §3650* §5470*
Point 2:  Allowance Range Check Point 2:  Allowance Range Check
Point 3:  Point 3: 
Point 4:   Reagent Blank Point 4:   Reagent Blank
Point 5:   Calibration Point 5:   Calibration
Point 6:  Point 6: 
Point 7  Advanced Calibration Point 7  Advanced Calibration
Point 8  Operation #  Point 8  Operation # 
Point 9  Point 9 
Point 10  Interval (RB/ACAL) #  Point 10  Interval (RB/ACAL) # 
<Point Cal. For No. of Correction Points  Use Master Curve   Lot Calibration <Point Cal. For No. of Correction Points  Use Master Curve   Lot Calibration
Master Curve> OD Range Master Curve> OD Range
Calibrator OD Conc Low High Stability Calibrator OD Conc Low High Stability
Point-1  Reagent Blank Day Hour Point-1  Reagent Blank Day Hour
Point-2  Calibration Day Hour Point-2  Calibration Day Hour
MB Type Factor: # 1-Point Calibration Point   with Conc-0 MB Type Factor: # 1-Point Calibration Point   with Conc-0

Enzyme BSOSR6x09.07
2018-05
AST (IFCC, with OSR60180 activition) AU5800 Paediatric Application
System Reagent: OSR6009, OSR6109, OSR6209, OSR6609 Reagent ID: 009
Parameters Specific Test Parameters
General LIH ISE HbA1c Calculated Test Range

Test Name: ASTP    Type: Serum  Operation Yes 

Sample Volume 5 L Dilution 10 L OD Limit

Pre-Dilution Rate 1  Diluent Bottle #  Min.OD 1.0 Max.OD 2.5
Rgt. Volume R1(R1-1) 25 L Dilution 10 L Reagent OD Limit
R1-2 5 L Dilution 10 L 1st. Low 1.3 High 2.5
Last Low 1.3 High 2.5
R2(R2-1) 25 L Dilution 40 L
Dynamic Range Low 3* High 1000*
Common Rgt. Type R1-2 Name # Correlation Factor A 1 B 0
Wavelength Pri 340 nm Sec. 660 nm Factor for Maker A 1 B 0
Method RATE 
Reaction Slope -  Onboard Stability Period 30 Day 0 Hour
Measuring Point1 1st 14 Last 27 LIH Influence Check # 
Measuring Point2 1st Last Lipemia +++++ 
Linearity Limit 15 % Icterus +++++ 
Lag Time Check YES  Hemolysis + 

Parameters Specific Test Parameters

General LIH ISE HbA1c Calculated Test Range

Test Name: ASTP    Type: Serum 

Value/Flag: # 
Level Low # High #
Specific Ranges: From To
Sex Year Month Year Month Low High
 1. #  # # # # # #
 2. #  # # # # # #
 3. #  # # # # # #
 4. #  # # # # # #
 5. #  # # # # # #
 6. #  # # # # # #
7. Standard demographics # #
8. Not within expected values # #
Panic Value Low # High # Unit U/L* Decimal Places #
Parameters Calibration Parameters
Calibrators Calibration Specific
General ISE

Test Name: ASTP    Type Serum  Cuvette . 

□ Use Serum Cal.

Calibration Type: MB  Formula: Y=AX+B  Counts: # 

<Calibrator Parameters> Range
Calibrator OD Conc Low High Slope Check None 
Point 1: §  § §3650* §5470*
Point 2:  Allowance Range Check
Point 3: 
Point 4:  □ Reagent Blank
Point 5:  □ Calibration
Point 6: 
Point 7:  Advanced Calibration
Point 8:  Operation # 
Point 9: 
Point 10:  Interval (RB/ACAL) # 
<Point Cal. For No. of Correction Points  Use Master Curve  □ Lot Calibration
Master Curve> OD Range
Calibrator OD Conc Low High Stability
Point-1  Reagent Blank Day Hour # User defined
Point-2  Calibration Day Hour * Values set for working in U/L. To work in SI units (µkat/L) divide by 60
MB Type Factor: # 1-Point Calibration Point  □ with Conc-0 § For use in AB mode only. Refer to IFU for further instruction

Enzyme BSOSR6x09.07
2018-05
 Name: AST (IFCC,without Pyridoxal phosphate activation), DxC 700 AU Serum/Plasma Application
System Reagent: OSR6x09
Test name: AST1N Reagent ID: 009

General LIH ISE Calculated Test Range

Test Name: ▼ Type: ▼ Operation ▼
AST1N Test No Serum Yes

Sample Volume 5.0 µL Dilution 0 ▼ µL OD Limit

Pre-Dilution Rate 1 ▼ Min. OD 0.6000 Max OD 2.5000
Reagent OD Limit
Reagent Volume R1 (R1-1) 25 µL Dilution 25 µL 1st Low 1.1000 High 2.5000
R1-2 µL Dilution µL Last Low 1.1000 High 2.5000
R2 (R2-1) 25 µL Dilution 50 µL Analytical Measuring Range Low 3.00* High 1000.00*
Common Reagent Type None Name None Correlation Factor A 1 B 0

Wavelength Pri 340 ▼ nm Sec 660 ▼ nm Manufacturer Factor A 1 B 0

Method RATE ▼
Reaction Slope - ▼ Onboard Stability Period 30 Day 0 Hour
Measuring Point-1 1st 14 Last 27 LIH Influence Check # ▼
Measuring Point-2 1st Last Lipemia +++++ ▼
Linearity Limit 15 % Icterus +++++ ▼
Lag Time Check YES ▼ Hemolysis + ▼

General LIH ISE Calculated Test Range

Test Name: ▼ Type: ▼
AST1N Test No Serum

Value/Flag # ▼ Level Low # High #

Specific Ranges
From To
Sex Year Month Year Month Other Type Low High
□ 1: # ▼ # # # # None # #

□ 2: # ▼ # # # # None # #

□ 3: # ▼ # # # # None # #

□4 # ▼ # # # # None # #

□ 5: # ▼ # # # # None # #

□ 6: # ▼ # # # # None # #

7: Standard demographics # #

8: Not within expected values # #

Critical Limits Low # High # Unit U/L* Select Decimal Places #

Calibrators General ISE  

Test Name: ▼ Type: ▼
AST1N Serum
□ Use Serum Cal.
Calibration Type: MB ▼ Formula: Y=AX+B ▼ Counts: # ▼

<Calibrator Parameters> Slope Check ▼

Range
Calibrator OD Conc
Low High
Point-1 § ▼ § §3650* §5470* Allowable Range Check
Point-2 ▼ □ Reagent Blank
Point-3 ▼ □ Calibration
Point-4 ▼ Advanced Calibration
Point-5 ▼ Operation Yes ▼
Point-6 ▼
Point-7 ▼ Interval (RB) Lot ▼

MB Type Factor # 1-Point Calibration Point ▼ □ with Conc-0 Interval (ACAL) ▼

Stability
Reagent Blank Day Hour

Calibration Day Hour

# User Defined
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60
§ For use in AB mode only, refer to IFU for further instruction

Enzyme BSOSR6x09.07
2018-05
 Name: AST (IFCC,with 60106 activation), DxC 700 AU Serum/Plasma Application
System Reagent: OSR6x09
Test name: AST2N Reagent ID: 009

General LIH ISE Calculated Test Range

Test Name: ▼ Type: ▼ Operation ▼
AST2N Test No Serum Yes

Sample Volume 5.0 µL Dilution 0 ▼ µL OD Limit

Pre-Dilution Rate 1 ▼ Min. OD 1.0000 Max OD 2.5000
Reagent OD Limit
Reagent Volume R1 (R1-1) 25 µL Dilution 25 µL 1st Low 1.3000 High 2.5000
R1-2 µL Dilution µL Last Low 1.3000 High 2.5000
R2 (R2-1) 25 µL Dilution 50 µL Analytical Measuring Range Low 3.00* High 1000.00*
Common Reagent Type None Name None Correlation Factor A 1 B 0

Wavelength Pri 340 ▼ nm Sec 660 ▼ nm Manufacturer Factor A 1 B 0

Method RATE ▼
Reaction Slope - ▼ Onboard Stability Period 7 Day 0 Hour
Measuring Point-1 1st 14 Last 27 LIH Influence Check # ▼
Measuring Point-2 1st Last Lipemia +++++ ▼
Linearity Limit 15 % Icterus +++++ ▼
Lag Time Check YES ▼ Hemolysis + ▼

General LIH ISE Calculated Test Range

Test Name: ▼ Type: ▼
AST2N Test No Serum

Value/Flag # ▼ Level Low # High #

Specific Ranges
From To
Sex Year Month Year Month Other Type Low High
□ 1: # ▼ # # # # None # #

□ 2: # ▼ # # # # None # #

□ 3: # ▼ # # # # None # #

□4 # ▼ # # # # None # #

□ 5: # ▼ # # # # None # #

□ 6: # ▼ # # # # None # #

7: Standard demographics # #

8: Not within expected values # #

Critical Limits Low # High # Unit U/L* Select Decimal Places #

Calibrators General ISE  

Test Name: ▼ Type: ▼
AST2N Serum
□ Use Serum Cal.
Calibration Type: MB ▼ Formula: Y=AX+B ▼ Counts: # ▼

<Calibrator Parameters> Slope Check ▼

Range
Calibrator OD Conc
Low High
Point-1 § ▼ § §3650* §5470* Allowable Range Check
Point-2 ▼ □ Reagent Blank
Point-3 ▼ □ Calibration
Point-4 ▼ Advanced Calibration
Point-5 ▼ Operation Yes ▼
Point-6 ▼
Point-7 ▼ Interval (RB) Lot ▼

MB Type Factor # 1-Point Calibration Point ▼ □ with Conc-0 Interval (ACAL) ▼

Stability
Reagent Blank Day Hour

Calibration Day Hour

# User Defined
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60
§ For use in AB mode only, refer to IFU for further instruction

Enzyme BSOSR6x09.07
2018-05
 Name: AST (IFCC, with OSR60180 activation), DxC 700 AU Serum/Plasma Application
System Reagent: OSR6x09
Test name: AST3N Reagent ID: 009

General LIH ISE Calculated Test Range

Test Name: ▼ Type: ▼ Operation ▼
AST3N Test No Serum Yes

Sample Volume 5.0 µL Dilution 0 ▼ µL OD Limit

Pre-Dilution Rate 1 ▼ Min. OD 1.0000 Max OD 2.5000
Reagent OD Limit
Reagent Volume R1 (R1-1) 25 µL Dilution 10 µL 1st Low 1.3000 High 2.5000
R1-2 5 µL Dilution 10 µL Last Low 1.3000 High 2.5000
R2 (R2-1) 25 µL Dilution 50 µL Analytical Measuring Range Low 3.00* High 1000.00*
Common Reagent Type R1-2 Name # Correlation Factor A 1 B 0

Wavelength Pri 340 ▼ nm Sec 660 ▼ nm Manufacturer Factor A 1 B 0

Method RATE ▼
Reaction Slope - ▼ Onboard Stability Period 30 Day 0 Hour
Measuring Point-1 1st 14 Last 27 LIH Influence Check # ▼
Measuring Point-2 1st Last Lipemia +++++ ▼
Linearity Limit 15 % Icterus +++++ ▼
Lag Time Check YES ▼ Hemolysis + ▼

General LIH ISE Calculated Test Range

Test Name: ▼ Type: ▼
AST3N Test No Serum

Value/Flag # ▼ Level Low # High #

Specific Ranges
From To
Sex Year Month Year Month Other Type Low High
□ 1: # ▼ # # # # None # #

□ 2: # ▼ # # # # None # #

□ 3: # ▼ # # # # None # #

□4 # ▼ # # # # None # #

□ 5: # ▼ # # # # None # #

□ 6: # ▼ # # # # None # #

7: Standard demographics # #

8: Not within expected values # #

Critical Limits Low # High # Unit U/L* Select Decimal Places #

Calibrators General ISE  

Test Name: ▼ Type: ▼
AST3N Serum
□ Use Serum Cal.
Calibration Type: MB ▼ Formula: Y=AX+B ▼ Counts: # ▼

<Calibrator Parameters> Slope Check ▼

Range
Calibrator OD Conc
Low High
Point-1 § ▼ § §3650* §5470* Allowable Range Check
Point-2 ▼ □ Reagent Blank
Point-3 ▼ □ Calibration
Point-4 ▼ Advanced Calibration
Point-5 ▼ Operation Yes ▼
Point-6 ▼
Point-7 ▼ Interval (RB) Lot ▼

MB Type Factor # 1-Point Calibration Point ▼ □ with Conc-0 Interval (ACAL) ▼

Stability
Reagent Blank Day Hour

Calibration Day Hour

# User Defined
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60
§ For use in AB mode only, refer to IFU for further instruction

Enzyme BSOSR6x09.07
2018-05
 Name: AST (IFCC,without Pyridoxal phosphate activation), DxC 700 AU Paediatric Serum Application
System Reagent: OSR6x09
Test name: AST1NP Reagent ID: 009

General LIH ISE Calculated Test Range

Test Name: ▼ Type: ▼ Operation ▼
AST1NP Test No Serum Yes

Sample Volume 5.0 µL Dilution 10 ▼ µL OD Limit

Pre-Dilution Rate 1 ▼ Min. OD 0.6000 Max OD 2.5000
Reagent OD Limit
Reagent Volume R1 (R1-1) 25 µL Dilution 25 µL 1st Low 1.1000 High 2.5000
R1-2 µL Dilution µL Last Low 1.1000 High 2.5000
R2 (R2-1) 25 µL Dilution 40 µL Analytical Measuring Range Low 3.00* High 1000.00*
Common Reagent Type None Name None Correlation Factor A 1 B 0

Wavelength Pri 340 ▼ nm Sec 660 ▼ nm Manufacturer Factor A 1 B 0

Method RATE ▼
Reaction Slope - ▼ Onboard Stability Period 30 Day 0 Hour
Measuring Point-1 1st 14 Last 27 LIH Influence Check # ▼
Measuring Point-2 1st Last Lipemia +++++ ▼
Linearity Limit 15 % Icterus +++++ ▼
Lag Time Check YES ▼ Hemolysis + ▼

General LIH ISE Calculated Test Range

Test Name: ▼ Type: ▼
AST1NP Test No Serum

Value/Flag # ▼ Level Low # High #

Specific Ranges
From To
Sex Year Month Year Month Other Type Low High
□ 1: # ▼ # # # # None # #

□ 2: # ▼ # # # # None # #

□ 3: # ▼ # # # # None # #

□4 # ▼ # # # # None # #

□ 5: # ▼ # # # # None # #

□ 6: # ▼ # # # # None # #

7: Standard demographics # #

8: Not within expected values # #

Critical Limits Low # High # Unit U/L* Select Decimal Places #

Calibrators General ISE  

Test Name: ▼ Type: ▼
AST1NP Serum
□ Use Serum Cal.
Calibration Type: MB ▼ Formula: Y=AX+B ▼ Counts: # ▼

<Calibrator Parameters> Slope Check ▼

Range
Calibrator OD Conc
Low High
Point-1 § ▼ § §3650* §5470* Allowable Range Check
Point-2 ▼ □ Reagent Blank
Point-3 ▼ □ Calibration
Point-4 ▼ Advanced Calibration
Point-5 ▼ Operation Yes ▼
Point-6 ▼
Point-7 ▼ Interval (RB) Lot ▼

MB Type Factor # 1-Point Calibration Point ▼ □ with Conc-0 Interval (ACAL) ▼

Stability
Reagent Blank Day Hour

Calibration Day Hour

# User Defined
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60
§ For use in AB mode only, refer to IFU for further instruction

Enzyme BSOSR6x09.07
2018-05
 Name: AST (IFCC, with 60106 activation), DxC 700 AU Paediatric Serum Application
System Reagent: OSR6x09
Test name: AST2NP Reagent ID: 009

General LIH ISE Calculated Test Range

Test Name: ▼ Type: ▼ Operation ▼
AST2NP Test No Serum Yes

Sample Volume 5.0 µL Dilution 10 ▼ µL OD Limit

Pre-Dilution Rate 1 ▼ Min. OD 1.0000 Max OD 2.5000
Reagent OD Limit
Reagent Volume R1 (R1-1) 25 µL Dilution 25 µL 1st Low 1.3000 High 2.5000
R1-2 µL Dilution µL Last Low 1.3000 High 2.5000
R2 (R2-1) 25 µL Dilution 40 µL Analytical Measuring Range Low 3.00* High 1000.00*
Common Reagent Type None Name None Correlation Factor A 1 B 0

Wavelength Pri 340 ▼ nm Sec 660 ▼ nm Manufacturer Factor A 1 B 0

Method RATE ▼
Reaction Slope - ▼ Onboard Stability Period 7 Day 0 Hour
Measuring Point-1 1st 14 Last 27 LIH Influence Check # ▼
Measuring Point-2 1st Last Lipemia +++++ ▼
Linearity Limit 15 % Icterus +++++ ▼
Lag Time Check YES ▼ Hemolysis + ▼

General LIH ISE Calculated Test Range

Test Name: ▼ Type: ▼
AST2NP Test No Serum

Value/Flag # ▼ Level Low # High #

Specific Ranges
From To
Sex Year Month Year Month Other Type Low High
□ 1: # ▼ # # # # None # #

□ 2: # ▼ # # # # None # #

□ 3: # ▼ # # # # None # #

□4 # ▼ # # # # None # #

□ 5: # ▼ # # # # None # #

□ 6: # ▼ # # # # None # #

7: Standard demographics # #

8: Not within expected values # #

Critical Limits Low # High # Unit U/L* Select Decimal Places #

Calibrators General ISE  

Test Name: ▼ Type: ▼
AST2NP Serum
□ Use Serum Cal.
Calibration Type: MB ▼ Formula: Y=AX+B ▼ Counts: # ▼

<Calibrator Parameters> Slope Check ▼

Range
Calibrator OD Conc
Low High
Point-1 § ▼ § §3650* §5470* Allowable Range Check
Point-2 ▼ □ Reagent Blank
Point-3 ▼ □ Calibration
Point-4 ▼ Advanced Calibration
Point-5 ▼ Operation Yes ▼
Point-6 ▼
Point-7 ▼ Interval (RB) Lot ▼

MB Type Factor # 1-Point Calibration Point ▼ □ with Conc-0 Interval (ACAL) ▼

Stability
Reagent Blank Day Hour

Calibration Day Hour

# User Defined
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60
§ For use in AB mode only, refer to IFU for further instruction

Enzyme BSOSR6x09.07
2018-05
 Name: AST (IFCC,with OSR60180 activation), DxC 700 AU Serum/Plasma Paediatric Application
System Reagent: OSR6x09
Test name: AST3NP Reagent ID: 009

General LIH ISE Calculated Test Range

Test Name: ▼ Type: ▼ Operation ▼
AST3NP Test No Serum Yes

Sample Volume 5.0 µL Dilution 10 ▼ µL OD Limit

Pre-Dilution Rate 1 ▼ Min. OD 1.0000 Max OD 2.5000
Reagent OD Limit
Reagent Volume R1 (R1-1) 25 µL Dilution 10 µL 1st Low 1.3000 High 2.5000
R1-2 5 µL Dilution 10 µL Last Low 1.3000 High 2.5000
R2 (R2-1) 25 µL Dilution 40 µL Analytical Measuring Range Low 3.00* High 1000.00*
Common Reagent Type R1-2 Name # Correlation Factor A 1 B 0

Wavelength Pri 340 ▼ nm Sec 660 ▼ nm Manufacturer Factor A 1 B 0

Method RATE ▼
Reaction Slope - ▼ Onboard Stability Period 30 Day 0 Hour
Measuring Point-1 1st 14 Last 27 LIH Influence Check # ▼
Measuring Point-2 1st Last Lipemia +++++ ▼
Linearity Limit 15 % Icterus +++++ ▼
Lag Time Check YES ▼ Hemolysis + ▼

General LIH ISE Calculated Test Range

Test Name: ▼ Type: ▼
AST3NP Test No Serum

Value/Flag # ▼ Level Low # High #

Specific Ranges
From To
Sex Year Month Year Month Other Type Low High
□ 1: # ▼ # # # # None # #

□ 2: # ▼ # # # # None # #

□ 3: # ▼ # # # # None # #

□4 # ▼ # # # # None # #

□ 5: # ▼ # # # # None # #

□ 6: # ▼ # # # # None # #

7: Standard demographics # #

8: Not within expected values # #

Critical Limits Low # High # Unit U/L* Select Decimal Places #

Calibrators General ISE  

Test Name: ▼ Type: ▼
AST3NP Serum
□ Use Serum Cal.
Calibration Type: MB ▼ Formula: Y=AX+B ▼ Counts: # ▼

<Calibrator Parameters> Slope Check ▼

Range
Calibrator OD Conc
Low High
Point-1 § ▼ § §3650* §5470* Allowable Range Check
Point-2 ▼ □ Reagent Blank
Point-3 ▼ □ Calibration
Point-4 ▼ Advanced Calibration
Point-5 ▼ Operation Yes ▼
Point-6 ▼
Point-7 ▼ Interval (RB) Lot ▼

MB Type Factor # 1-Point Calibration Point ▼ □ with Conc-0 Interval (ACAL) ▼

Stability
Reagent Blank Day Hour

Calibration Day Hour

# User Defined
* Values set for working in U/L. To work in SI units (µkat/L) divide by 60
§ For use in AB mode only, refer to IFU for further instruction

Enzyme BSOSR6x09.07
2018-05