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CO2IncubatorCare BestPractices

CO2 incubators are designed to replicate in vivo conditions for optimal cell and tissue growth by controlling carbon dioxide, humidity, and temperature levels in a contamination-free environment. Cells and tissues are sensitive to environmental changes, so proper maintenance of the CO2 incubator is important.

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14 views1 page

CO2IncubatorCare BestPractices

CO2 incubators are designed to replicate in vivo conditions for optimal cell and tissue growth by controlling carbon dioxide, humidity, and temperature levels in a contamination-free environment. Cells and tissues are sensitive to environmental changes, so proper maintenance of the CO2 incubator is important.

Uploaded by

Asistencia Técnica
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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CO2 incubators are designed to replicate in vivo conditions for optimal growth of cells and tissues.

The device controls the levels of carbon


dioxide, humidity, and temperature in a contamination-free environment. Cells and tissues are highly sensitive to changes in their environment,
so it is important to keep your CO2 incubator functioning properly.

Avoid Maintain Optimal


Contamination Temperature
Always wear gloves when using CO2 incubators are typically
the incubator and keep the inner maintained at 37°C. Attach
glass door closed as much as a calibrated thermometer to
possible. Change the water in the the inside of the glass door to
humidity pan weekly; regularly enable temperature readings
clean the pan, shelves, chamber, from the outside when the door
and any passages into the is closed. Check periodically that
incubator; and occasionally wipe the temperature sensor matches
down the door handle. the thermometer reading, and
recalibrate the sensor if the
readings differ.

Monitor Keep
CO2 Humidity High
The CO2 concentration should If the humidity inside of the
be kept around 5% to match incubator falls too low, the
physiological conditions and cell culture can evaporate or
to maintain a constant pH. growth media can become too
While most CO2 incubators are concentrated. To keep humidity
equipped with a CO2 sensor, an around 95%, make sure that there
external gas analyzer will help to is always water in the tray under
ensure the readings are accurate. the incubator.

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