CLINICAL CHEMISTRY

ANALYZER

AE-600N

SERVICE MANUAL

ERMA INC., JAPAN

 Contents
Sr no. Descriptions Pg no.

1. Steps for Installation 1

2. Maintenance and Instrument 1

3. Testing Procedure 2

4. MCB Board 3

5. Replacement of Inlet Tubings & Outlet 4

6. Replacement Of Peristaltic Pump 5

7. Photometer Assembly 6 to 7

8. Trouble Shooting 8 to 13

9. AE-600N Spare Parts 14 &15

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SAFETY NOTES TO USERS
Be sure to read "Safety notes to users" before you use this instrument for operation.
Symbols and their meaning are as follows:

If treated mistakenly, a danger to cause death or wound is expected to be

DANGER
eminent.

If treated mistakenly , a possibility to cause death or wound is expected to

WARNING
be eminent.

If treated mistakenly , a danger to cause wound and physical damage is

CAUTION
expected to happen.

Read the statement following the symbol. The statement is alerting you to a
BIOHAZARD
potentially bio-hazardous condition.

WARNING

About the operator

This instrument must be operated by nationally qualified doctors or technicians who is

well skilled to use machine.

About diagnostics

Clinical diagnosis based on the measurement result and its treatment must to be
determined together with clinical symptoms and other checkup results by a doctor in
charge of the patient.

Take ground

Unless it is done ,it may cause an electrical shock when breakdown and electrical power
leaking occure.

3
Power unit outlet should be independent

Place instrument where independent power receptacle can be used. use receptacle
different from the one used by a device that easily generate noise such as a centrifuge
etc.

Not to handle with wet hands

Never plug or unplug with wet hands.It may cause electrical shock.

When unplugged,it should not be pulled with a cable.

Pull a plug out by holding a plug with two fingers , or it may cause electrical shock or
short to bring about fire.

Never use other cords and cables than the designated ones.

Cords and cables with less electrical capacity may cause fire.Do not step on,twist or pull
cords.Never use damaged power cords.

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 BIOHAZARD

Mishandling of samples

Not to touch samples directly

Follow standard safety procedure

5
Disposal of waste

Disposal of the waste in accordance with as and on applicable rules for Bio-hazard waste
disposal and consulting the manufacturer or distributor of the reagents for details.

CAUTION

Use and operate the unit in accordance with enclosed "User's guide"

Perform operations cautiously when using Reagents and wash solutions.

Warranty:
Warranty does not cover any defect, malfunctioning or damage due to: a) Usage under
the condition prohibited by the instruction. b) Customer does not do proper maintenance
required by the instruction manual. c) Customer disassembles the instrument without
permission from manufacturer/selling agencies d) Usage of generic reagents. e)
Instrument opened or serviced other than authorized person.
Note: Instrument can not be installed without proper Stabilized voltage, Earthing and
Proper environmental conditions. If fail to fulfil or providing false information will be
harmful for the instrument.

6
Steps for Installation
1.Check groundings of the power supply:

 Before Installing the Analyzer, it is necessary to check for good mains supply.
 It is important to have a good earthing since poor earthing causes variation in the
results which will affect the performance of the machine.
 Phase (Left side in the socket) to Neutral (Right side in the socket) = 110/220Vac (±
10Vac) without fluctuation.
 Phase (Left side in the socket) to Earth (Top side in the socket) = 110/220Vac (± 5Vac)
without fluctuation.
 Neutral (Right side in the socket) to Earth (Top side in the socket) = not more than
± 5Vac.

Environmental conditions for the analyzer:

 The ambient temperature should be from 15- 40ºC.

 Instrument should be placed away from direct sunlight, dust free & well-
ventilated environment.
 Instrument should not be placed near any device generating high frequency wave.

2.Maintenance and Instrument

Care Daily Maintenance:
 Wipe off any reagent spills on the instrument immediately.
 After every Chemistry run, wash the flowcell thoroughly with good quality
Distilled / Deionised water using the aspiration switch.
 Always leave the flowcell filled with distilled / deionised water. Never leave any
reaction mixture in it.
 Empty the waste solution bottle every evening.

Caution:
 Do not use strong Acidic or Alkaline solutions which can cause irrepairable damage to
the instrument.
 Do not leave the Flowcell dry. Always leave it filled with distilled / deionised water.

NOTE: It is advisable to carry out the PERISTALTIC PUMP CALIBRATION

every week in order to compensate for the change in elasticity of the tubing.

7
 3.Testing procedure :-
I. SMPS:-
 Input Voltage :- 100 to 240Vac, 50/60Hz, ±10%
Output Voltage :- 12.3Vdc, +0.2Vdc
 Mains fuse :- 2A, 250Vac, (Slow blow)
II. Main Controller Board (AGDSAMCB-0C/1D/1E)
Test point voltages
Sr. No. TP # (w.r.t. GND) Voltage range Condition
1. 5V 4.9 to 5.3Vdc Power ON, LED D21 glow.
2. 3V3 3.1 to 3.3Vdc Power ON, LED D20 glow.
After booting the Instrument. LED D27
3. LV (Lamp) 5.5 to 6.1Vdc
glow.
2.9 to 3.0Vdc “Check absorbance” / “Analyze” mode,
4. PV (Peltier) (Toggling state) LED D26 & D15/D16 glow.
After booting the Instrument or during
5. 8V 7.5 to 8.2Vdc Printout, LED D22 glow.
6. Coin Cell 3Vdc Always
During stepper motor locked/rotate. LED
7. Across R2/R3 0.1 to 0.4
D1/D3 & D5/D8 LED glow.
> 3V Filter wheel plate slot not in the opto path.
8. FWO (J3) Pin 2 Filter wheel plate slot in the opto path,
< 0.5V LED D36 glow
> 3V Aspiration switch without press position.
9. OPT (J15) Pin 2 Aspiration switch press position, LED D37
< 0.5V glow
During peristaltic dc pump on, LED D9
10. Across V1 (J4) > 11V glow.
< 0.5V Aspiration switch press position.
± 2.9 to 3.1V
(AGDSAMCB-0C) “Check absorbance” / “Analyze” mode.
± 2.9 to 7V Cooling action - LED D13 & D16 glow.
Across V4/PLTR
(AGDSAMCB- Hot action - LED D15 glow
11. (J8) 1D/E)
Exit from “Check absorbance” / “Analyze”
< ±0.2V mode.
LED D26 & D15/D16 off.
Thermal PRT. (J21)
12. 4.9 to 5.3Vdc After booting the Instrument.
Pin 2
Thermal PRT. (J21) After booting the Instrument or during
13. Pin 3 7.5 to 8.2Vdc Printout.
Thermal PRT. (J21) After booting the Instrument or during
14. -5.0 to -12.0Vdc
Pin 4 Printout.
15. PHM (J23) Pin 1 3.1 to 3.3Vdc Power ON.
16. PHM (J23) Pin 2 3.1 to 3.3Vdc Power ON.
17. PHM (J23) Pin 3 3.1 to 3.3Vdc Power ON.
18. USB (J22) pin 1 & 2 4.9 to 5.3Vdc Power ON.
19. USB (J22) pin 3 & 4 < 0.5V Device (Keyboard / Mouse) not connected.

8
> 2.7V Device (Keyboard / Mouse) connected.

9
4.Replacement of tubings:
Replace the aspiration and peristaltic pump tubing if it shows signs of wear and tear.

Replacement of Aspiration tubing:

 Open the Flowcell flap, gently remove the flowcell from its slot.
 Unscrew the aspiration tubing from the flowcell.
 Slide out the connector, O-ring and washer of the old aspiration tubing.
 Slide in the connector, O-ring and washer into the new aspiration tubing.
 Check for proper flanging of new tubing, if not then rub it on a clean flat surface.
 Screw it to the flowcell.
 Place the flowcell back into its slot.
 Aspirate deionised water.
 Close the flowcell flap cover.

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5.Replacement of peristaltic Pump:

 Unscrew the machine's top cover.

 Replace the Pump with new Pump Casstte (with tubing)
 Lock it with the bush buttons.
 Connect the first end to the flowcell outlet tubing and the other end to the waste
tubing.
 Perform pump calibration with 1 mL Deionised water.

Note: After replacement of the Pump, it is essential to recalibrate the peristaltic pump.

Internal view of Peristaltic Pump

External view of Peristaltic pump

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6.Photometer Assembly:
Lamp Assembly :

Photo micro sensor is to control the home position of filterwheel.

Lamp Replacement Procedure :

 Switch OFF the instrument, unplug the power cord.

 Open the Machine's top cover, remove lamp connector.
 Unscrew the old lamp.
 Replace the lamp with the new one, wipe it with a clean lintfree tissue.
 Connect the lamp connector.
 Connect power cord, switch ON the machine.
 Click on 'Service' Icon, select 'Absorbance Mode'.
 Select 340nm filter.
 Aspirate Deionised/ Distilled water, check the Absorbance and Count (Abs. should
be around ± 0.00-0.01 and Count should be minimum 17000).
 Adjust the lamp height using Allen keys.

NOTE:
 Lamp voltage should be 5.5 – 5.8 Volts.(More voltage will affect the lamp life.)
Lens adjustment should be disturbed only if the counts are not adjusted by lamp
adjustment.

Photodiode PCB : Converts Light into electrical signal.

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Trouble Shooting for Instrument:

 If machine crashes :- Internal action not completed as expected during sample analysis
or while saving the desired changes.
 Could not Save Date & Time :- Date & Time Could not be set to RTC.

NOTE: If the above things happen then attach a pendrive, go to 'Set Up' option, Copy
Log and send to HO.

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 Troubleshooting
Problem Cause Action
Instrument doesn’t  Mains supply not proper.  Loose connection at Mains
ON or ON-OFF switch/socket/mains cord.
frequently.  Loose connection.
 Fuse absent/blown.
Display touch not  Display over tightened to  Slightly loosen the display fitting.
working. the instrument body.
 Perform touchscreen calibration using
 FPC cable connection not a mouse.
proper.
 Refit/ change FPC.
 Display not calibrated.

Touch screen may be

damaged.

Display working not  FPC cable connection not  Slightly tighten the display.
proper. proper.
 Refit / change FPC.
 Due to vibrations
developed because of  Don't place any other device
filter wheel rotation or generating high frequency waves near
some other high the analyzer.
frequency device placed
on the same platform.  Change display

Date / Time changes  Coin battery life may be  Change the coin battery.
after power ON over.
 Clean the clip contact / re-solder socket
 Loose connection in battery pins.
socket clip/solder pins.

Fluctuation in test  Noise in Mains Earth.  Provide noise free Earth mains supply.
results.
 Bubble in liquid path.  Clean flowcell window externally.

 Particle trapped in flowcell.  Check the liquid path and aspiration

tubing fitting with flowcell for bubble.
 Dust particle on flowcell
window  Give flowcell cleaning using Wash
Solutoin.
 Dust in optical path
 Clean the dust with soft brush.
 Lamp alignment not proper.
 Check filter wheel opto setting.

 Check the Lamp pcb soldering

 Do lamp alignment setting.

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 Problem Cause Action
Liquid not aspirated  Micro switch not working  Check if the peristaltic pump tubing is
when the connected properly, if not reconnect
ASPIRATION correctly.
SWITCH is pressed
& no buzzer sound.  Perform Pump Calibration.

 Replace micro switch electrical

assembly.

In Self test /  Bubble in Flowcell.  Check the liquid path and flowcell
Photometer tubing fitting for bubble.
Calibration / In  Photometer gain count may
Analyze ( high water be less.Perform  Clean the external walls of flowcell with
blank absorbance) Photometer calibration if tissue paper.
done long back.
OR  Clean flowcell thoroughly with cleaning
 Filter wheel position not solution followed by deionised water.
Error related to filter / proper.
clean flowcell path.  Check filter wheel opto setting.
 Low intensity of lamp.
 Check filter wheel motor connection.
 Damaged Filter
 Check the Lamp pcb soldering.

 Change the Lamp if its life is over.

 Perform photometer calibration if done

long back.

 Do Lamp & lens alignment setting.

Liquid not moving  Liquid path may be  Ensure that pump tubing and
properly. blocked. aspiration tubing are properly
OR connected.
Peristaltic pump  Flowcell or pump tube
operates but liquid is may be punctured.  Check tubings for blockage, crimps, or
not aspirated. leaks.
 Pump rotation not proper.
 Check pump motor connector
connection.

 Replace tubings.

USB Mouse/  USB port may not be  Check USB port fitting.
Keyboard/ Pendrive properly fitted.
not working.  Check USB port connector.
 Check USB PCB
 Check USB dry solder or replace it.
 MCB board may be
damaged.  Replace MCB board.

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16
 Problem Cause Action
The graph of a  'Graph' option not  Select 'Graph' option as soon as
Kinetic chemistry is selected entered in 'Sample' screen to get graph
not printed along with printed for each sample.
result.
Printer printout is  Printer roll may be old/  Replace printer roll.
faint. bad quality.
OR  Make sure that the paper roll is
Printer does not work.  Printer roll not properly installed properly with the heat
installed. sensitive surface up.(the instrument
will not print if the paper is fed
reversed.)

 Always use thermal paper of

acceptable quality.

 Check printer locking arrangement.

While performing a  It should be borne in mind to Change

Kinetic or Fixed time Patient Id before running the next
chemistry, the Patient sample.
ID do not change
even after the
previous result is
printed and another
sample is aspirated.

Temperature attained  Flowcell not inserted  Ensure that the flowcell is completely
in the flowcell is not properly in its slot. inserted in its slot.
within ±0.1ºC of the
selected temperature.  Reagents were not  Refrigerated reagents should be
OR allowed to come to room allowed to come to room temperature
Flowcell Temp not temperature before use. before use.
proper.
 Temp pcb not connected  Press and fit Temp pcb & tight the
properly. fitting screw.

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18
Poorly reproducible  Aspiration of sample not  Check if the aspiration volume is
or erratic readings. done properly. correctly programmed and whether the
amount of liquid aspirated is as
 Contamination in programmed.
reagents.
 If it is found that the aspiration is not
 Contaminated test-tubes / proper, perform pump calibration.
pipette tips.
 Check expiry date of the reagents and
control material used.

 Always use clean labwares.

Problem Cause Action

Control / Sample  Wrong Programming.  Ensure that the procedures specified
values not within by the reagent manufacturer are
range.  Improper reconstitution. followed.

 Expired Reagents or  Check the wavelength, temperature,

controls. sample and reagent volumes, factor
and standard concentration. In case of
kinetic chemistries make sure that the
Delay time and Read time are correctly
programmed.

 Check reagents, controls and standards

for expiry.

 Ensure that water used for

reconstitution is of reagent grade
quality. i.e. Deionised / double distilled
water.
'Control Data not set  If control data is not set  Set control data in Quality Control
for selected test'. for selected test during Icon.
run.

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20
 Error messages / Causes Corrective Action
Flags
Temperature not  Getting lower or higher  Check peltier.
attaining results for kinetic or fixed
time chemisties.  Check temperature PCB contact with
flowcell housing
 Fluctuations observed for
temperature displayed in
analyze mode / check
absorbance mode.
'SD' flag shown  Non-linearity or deviation in  Check for micro bubbles.
graph.
 Sample may not be properly aspirated.

 Clean Flowcell with Wash solution

followed by deionised water

 Problem with the reagent.

 If the deviation is within 20% than we

can say that deviation is negligible.
'R' flagging is  For Decreasing reactions: if  Problem with the reagent.
shown for result the reagent absorbance is less
than the Initial Abs. in Test  Check for reagent Contamination/
Parameters. expiry date and reconstitution stability
(if applicable).
 For Increasing reactions: If
the reagent absorbance is  Check for proper storage condition of
greater than the Initial Abs. in reagent.
Test Parameters.
 Use fresh reagent and rerun.
 Reagent deteriorated

RL flagging  If reagent blank absorbance  Problem with the reagent.

is less than Reagent Abs.
Low in Test Parameters.  Check for reagent expiry date and
reconstitution stability (if applicable).

 Check for proper storage condition of

reagent.

 Use fresh reagent and rerun.

LH flagging  If the sample result is greater  Dilute the sample and rerun.
than the Linearity in Test
Parameters.  After rerunning the diluted sample,
report the result by multiplying with
 If the sample result is greater dilution factor.
than Max Delta Abs/min in
Test Parameters.

21
Error messages / Causes Corrective Action
Flags
RH flagging  If reagent blank absorbance  Problem with the reagent.
is greater than Reagent Abs.
Low in Test Parameters.  Check for reagent expiry date and
reconstitution stability (if applicable).

 Check for proper storage condition of

reagent.

 Use fresh reagent and rerun.

Substrate depletion  Sample crossing the linearity.  Dilute the sample and re-run.
('S' flagging is to be
displayed )  For Kinetic assays:  After rerunning the diluted sample,
For Decreasing reactions: if the report the result by multiplying with
reaction Abs. is less than the dilution factor.
Reaction Abs. in Test Parameters.

For Increasing reactions: If the

Reaction Abs. is greater than the
Reaction Abs. in Test Parameters.
Substrate depleted  Initial Absorbance field and  For increasing kinetic reactions enter
but 'S' flagging is Reaction Absorbance field 1.3 in Initial Absorbance as well as
not given. not entered. reaction absorbance field.

 For decreasing kinetic reactions, enter

0.7 in Initial Absorbance as well as
reaction absorbance field.

 For increasing fixed time reactions,

enter 1.3 in Initial Absorbance field.

 For decreasing kinetic reactions, enter

0.7 in Initial Absorbance field.
'R,S' flag is shown  Reagent detoriated as well as  Check Initial Abs. in case of increasing
for Result. substrate depleted. reaction if its greater than the value
entered in the Initial Abs. Field (i.e =
 Initial Abs. in case of 1.3) in Test Parameters then change the
increasing Kinetic / Fixed reagent and re-run.
time reaction will be greater
than the value entered in the  Check Initial Abs. in case of
Initial Abs. Field in Test decreasing reaction if its less than the
Parameters. value entered in the Initial Abs. Field
(i.e = 0.7) in Test Parameters then
 Initial Abs. in case of change the reagent and re-run.
decreasing Kinetic / Fixed
time reaction will be less than  Change the reagent and re-run.
the value entered in the Initial
 In 'Parameter' screen enter the Initial.
Abs. Field in Test Parameters. Abs As well as reaction Abs.

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 AE-600N Spare Parts
Sr no. Spare Part Name Item Code Description
1 Lamp Assembly AA000084

2 Pump Cassette with SS000093

tubing
3 DC Pump motor EA000001 EA DC PERISTALTIC PUMP ASSEMBLY
4 Filters- 340 MB000085 ROUND FILTER - 340 NM (DIA 10 X 8 T)
5 With ND filter 405 MB000086 ROUND FILTER - 405 NM (DIA 10 X 8 T)
6 With ND filter 450 MB000087 ROUND FILTER - 450 NM (DIA 10 X 8 T)
7 With ND filter 505 MB000088 ROUND FILTER - 505 NM (DIA 10 X 8 T)
8 With ND filter 546 MB000089 ROUND FILTER - 546 NM (DIA 10 X 8 T)
9 With ND filter 578 MB000090 ROUND FILTER - 578 NM (DIA 10 X 8 T)
10 With ND filter 630 MB000091 ROUND FILTER - 630 NM (DIA 10 X 8 T)
11 With ND filter 670 MB000092 ROUND FILTER - 670 NM (DIA 10 X 8 T)

12 Stepper Motor(Filter
Wheel) EA000009 EA STEPPER MOTOR ASSEMBLY

13 Optical Sensor for Filter

wheel EA000007 EA FILTER WHEEL OPTO ASSEMBLY
14 Flowcell 32ul MB000081 STARNA FLOWCELL

15 Flowcell tubing with Inlet FLOWCELL INLET CONNECTOR

connector AA000061 ASSEMBLY

16 Flowcell tubing with FLOWCELL OUTLET CONNECTOR

outlet connector AA000062 ASSEMBLY
17 Waste bottle with tubing Waste Bottle Assly (
18 Thermal Printer Head AA000036
19 CPU Board PA000015 PCB ASSEMBLY MCB 2020
20 SMPS unit-12V,75W EX000003 SMPS Universal INPUT 90-260VAC

21 7" Touch sreen Colour EA 7 INCH DISPLAY ASSEMBLY WITH

Display EA000019 TOUCH
22 Cable for display EW000015 FPC cable

23 STORAGE MEMORY CARD CLASS 4

SD card- 4 GB EI000041 FULL SDHC 8GB
24 Battery 3V EX000005 COIN CELL 3V CR2032
25 Aspiration Switch EA000008 EA ASPIRATION KEY ASSEMBLY
26 Peltier (2Pcs) EA000002 EA PELTIER ASSEMBLY 2020

27
Power entery module EA000005 MAIN SWITCH AND SOCKET ASSEMBLY
28 Fan EA000003 EA FAN ASSEMBLY
29 Photometer PCB PA000013 PCB ASSEMBLY PHOTOMETER 2020
30 Photometer AA000011 PHOTOMETER ASSEMBLY
31 Temperature PCB PA000009 PCB ASSEMBLY TEMP SENSOR 2020

32 PT Adaptor (For
Coagulation Test) AP000091 Coagulation tube adaptor
33 Cuvettes – 10 Nos. MB000083 CUVETTES

23
34 VARIABLE VOLUME MICRO PIPETTE (5-
Micro Pipette 5-50 FG000031 50ΜL)

35 VARIABLE VOLUME MICRO PIPETTE

Micro Pipette 100-1000 FG000032 (100-1000ΜL)
36 Timimg Belt MB000053 Timing Belt T5-51-255mm pl-6mm
37 Gusto Adaptor PA000025 PCB ASSEMBLY Adaptor
38 Toradex tiny ED000003 COLIBRI VF50 128 MB V1
39 Top panel AP000333 SEMI AUTO TOP CABINET
40 Product Logo AP000387 PRODUCT NUMBER DOME STICKER
41 Erma Logo AP000371 Erma LOGO
42 Lens mounting bracket AP000314 Lens MTG BRKT
Lens mounting bracket
43 AA000028 Lens BRACKET ASSEMBLY
assembly
44 Lens Bush AP000322 Lens Bush
45 Lens Assembly AA000029 Lens ASSEMBLY
46 Coagulation Tube MB000137 Coagtube
Graphical Printer EA ELEGANCE THERMAL PRINTER
47 EA000013
Assembly ASSEMBLY
48 LENS DIA 6.5 x EFL 7.8 AP000169 Lens
49 Tiny Board PCB ED000010 SBC tiny 6410
Waste Connector
50 MB00039 Panel mount Male Luer
of AE-600N
Waste Connector
51 MB00040 Panel mount NUT
of AE-600N

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