20 600 07625L - en - 2010/07

®
20 Strep IVD

Identification system for Streptococcaceae and related organisms

SUMMARY AND EXPLANATION - API 20 Strep Analytical Profile Index

API 20 Strep is a standardized system combining (Ref. 20 690)
20 biochemical tests that offer widespread capabilities. It or apiweb TM identification software (Ref. 40 011)
enables group or species identification of most (consult bioMérieux)
streptococci and enterococci, and those most common - Columbia blood agar plates (Ref. 43 041)
related organisms. The complete list of those organisms - Schaedler broth (optional)
that it is possible to identify with this system is given in the Material
Identification Table at the end of this package insert.
- Swabs
- Pipettes or PSIpettes
PRINCIPLE
- Ampule rack
The API 20 Strep strip consists of 20 microtubes - Ampule protector
containing dehydrated substrates for the demonstration of - Anaerobic jar
enzymatic activity or the fermentation of sugars. - General microbiology laboratory equipment
The enzymatic tests are inoculated with a dense
suspension of organisms, made from a pure culture, WARNINGS AND PRECAUTIONS
which is used to reconstitute the enzymatic substrates.
• For in vitro diagnostic use and microbiological
During incubation, metabolism produces color changes
control.
that are either spontaneous or revealed by the addition of
• For professional use only.
reagents.
• This kit contains products of animal origin. Certified
The fermentation tests are inoculated with an enriched
knowledge of the origin and/or sanitary state of the
medium which rehydrates the sugar substrates.
animals does not totally guarantee the absence of
Fermentation of carbohydrates is detected by a shift in the
transmissible pathogenic agents. It is therefore
pH indicator.
recommended that these products be treated as
The reactions are read according to the Reading Table
potentially infectious, and handled observing the usual
and the identification is obtained by referring to the
safety precautions (do not ingest or inhale).
Analytical Profile Index or using the identification software.
• All specimens, microbial cultures and inoculated
products should be considered infectious and handled
CONTENT OF THE KIT (Kit for 25 tests)
appropriately. Aseptic technique and usual precautions
- 25 API 20 Strep strips for handling the bacterial group studied should be
- 25 incubation boxes observed throughout this procedure. Refer to "CLSI®
- 25 ampules of API GP Medium M29-A, Protection of Laboratory Workers From
- 25 result sheets Occupationally Acquired Infections; Approved Guideline
- 1 package insert - Current revision". For additional handling precautions,
refer to "Biosafety in Microbiological and Biomedical
COMPOSITION Laboratories - CDC/NIH - Latest edition", or to the
Strip regulations currently in use in each country.
• Do not use reagents past the expiry date.
The composition of the API 20 Strep strip is given in the
• Before use, check that the packaging and components
Reading Table of this package insert.
are intact.
Medium • Do not use strips which have been damaged: cupules
deformed, desiccant sachet open, etc.
API GP L-cystine 0.5 g • It is recommended to perform a quality control test when
Medium Tryptone (bovine/porcine origin) 20 g a new ampule of ZYM B reagent is opened.
2 ml Sodium chloride 5g • Open ampules carefully as follows :
Sodium sulfite 0.5 g - Place the ampule in the ampule protector.
Phenol red 0.17 g - Hold the protected ampule in one hand in a
Demineralized water to make 1000 ml vertical position (white plastic cap upper-
pH : 7.4 - 7.6 most).
The quantities indicated may be adjusted depending on the titer - Press the cap down as far as possible.
of the raw materials used. - Position the thumb tip on the striated part of
the cap and press forward to snap off the
REAGENTS AND MATERIAL REQUIRED BUT NOT top of the ampule.
PROVIDED - Take the ampule out of the ampule protector
Reagents / Instrumentation and put the protector aside for subsequent
use.
- API® Suspension Medium, 2 ml (Ref. 70 700) - Carefully remove the cap.
- Reagents : NIN (Ref. 70 491) • The performance data presented were obtained using
VP 1 + VP 2 (Ref. 70 422) the procedure indicated in this package insert. Any
ZYM A (Ref. 70 494) change or modification in the procedure may affect the
ZYM B (Ref. 70 493) results.
- Mineral oil (Ref. 70 100)
- McFarland Standard (Ref. 70 900) point 4 on the scale
or DENSIMAT (Ref. 99 234)

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api® 20 Strep 07625L - en - 2010/07

• Interpretation of the test results should be made taking • Make a dense suspension with a turbidity greater than
into consideration the patient history, the source of the 4 McFarland. This suspension must be used
specimen, colonial and microscopic morphology of the immediately after preparation.
strain and, if necessary, the results of any other tests
Inoculation of the strip
performed, particularly the antimicrobial susceptibility
patterns. • In the first half of the strip (tests VP to ADH), distribute
this suspension, avoiding the formation of bubbles (tilt
STORAGE CONDITIONS the strip slightly forwards and place the tip of the pipette
or PSIpette against the side of the cupule) :
The strips and media should be stored at 2-8°C until the - For the tests VP to LAP : distribute approximately
expiry date indicated on the packaging. 100 µl into each cupule.
- For the ADH test : fill the tube only.
SPECIMENS (COLLECTION AND PREPARATION) • In the second half of the strip (tests RIB to GLYG) :
API 20 Strep is not for use directly with clinical or other - Open an ampule of API GP Medium as indicated in
specimens. the paragraph "Warnings and Precautions" and trans-
The microorganisms to be identified must first be isolated fer the rest of the suspension into it (appr. 0.5 ml).
on a suitable culture medium according to standard Mix well.
microbiological techniques. - Distribute this new suspension into the tubes only.
• Fill the cupule of the underlined tests (ADH to GLYG)
INSTRUCTIONS FOR USE with mineral oil to form a convex meniscus.
Selection of colonies • Place the lid on the tray.
• Incubate at 36°C ± 2°C in aerobic conditions for
Once the microorganism to be identified has been isolated 4 - 4 ½ hours to obtain a first reading and for 24 hours
and verified to be a member of the family (± 2 hours) to obtain a second reading if required.
Streptococcaceae (Gram, catalase test) :
• Note the type of hemolysis on the result sheet
READING AND INTERPRETATION
(21st test).
• Pick a well-isolated colony (Note 1) and suspend it in Reading the strip
0.3 ml of sterile water. Homogenize well. After 4 hours of incubation :
• Flood a Columbia sheep blood agar plate (Note 2) with • Add the reagents :
this suspension (or aseptically swab the entire surface - VP test : 1 drop of each of VP 1 and VP 2.
of the agar). - HIP test : 2 drops of NIN.
• Incubate the plate for 24 hours (± 2 hours) at - PYRA, αGAL, ßGUR, ßGAL, PAL and LAP tests :
36°C ± 2°C in anaerobic conditions. 1 drop of each of ZYM A and ZYM B (*).
NOTE 1 : ß-hemolytic streptococci and enterococci (*) It is recommended to control each ampule of
produce sufficiently large colonies after 24 hours of ZYM B before using for the first time.
incubation. For other streptococci, it is preferable to select To do this, it is recommended to use the strain ATCC®
a colony after 48 hours of incubation. For fastidious 700400 indicated in the Quality Control paragraph in
strains (producing minute colonies after 48 hours), the order to eliminate any defective reagents.
following procedure is recommended : • Wait 10 minutes, then read the reactions by referring to
- Culture the colony in 1 ml of Schaedler broth at the Reading Table. If necessary, expose the strip to a
36°C ± 2°C for 5 hours. strong light (10 seconds with a 1000 W lamp) to
- Flood a Columbia sheep blood agar plate with the entire decolorize any excess reagents in tubes PYRA to LAP.
culture. Remove any excess liquid.
Reincubation is necessary in the following cases :
- Incubate the plate for 18-24 hours at 36°C ± 2°C in
- low discrimination ;
anaerobic conditions.
- unacceptable or doubtful profile ;
NOTE 2 : In the case of suspected pneumococci, it is - or if the following comment is given for the profile :
advisable to prepare 2 agar plates in order to obtain IDENTIFICATION NOT VALID
sufficient growth. BEFORE 24 HOURS OF INCUBATION
Preparation of the strip In this case, after 24 hours, reread the reactions ESC,
ADH, and RIB to GLYG. Do not reread the enzymatic
• Prepare an incubation box (tray and lid) and distribute reactions (HIP, PYRA, αGAL, ßGUR, ßGAL, PAL, LAP)
about 5 ml of distilled water or demineralized water and VP. Record all the reactions on the result sheet.
[or any water without additives or chemicals which may
release gases (e.g. Cl2, CO2, etc.)] into the honey- Interpretation
combed wells of the tray to create a humid atmosphere. Identification is obtained with the numerical profile.
• Record the strain reference on the elongated flap of the
• Determination of the numerical profile :
tray. (Do not record the reference on the lid as it may be
On the result sheet, the tests are separated into groups
misplaced during the procedure).
of 3 and a value of 1, 2 or 4 is indicated for each. By
• Remove the strip from its individual packaging.
adding together the values corresponding to positive
• Place the strip in the incubation box.
reactions within each group, a 7-digit profile number is
Preparation of the inoculum obtained.
• Open an ampule of API Suspension Medium (2 ml) as
indicated in the paragraph "Warnings and Precautions"
or use any tube containing 2 ml of distilled water without
additives.
• Using a swab, harvest all the culture from the previously
prepared subculture plate.

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• Identification : NOTE : The hemolytic reaction constitutes the 21st test ;

This is performed using the database (V 7.0) ß-hemolysis is considered as positive with a numerical
* with the Analytical Profile Index : value of 4. All other hemolytic reactions are considered as
- Look up the numerical profile in the list of profiles. negative with a numerical value of 0. Nevertheless, this
* with the apiweb TM identification software : test may be of discriminant value for the identification of
- Enter the 7-digit numerical profile manually via the certain species.
keyboard.

5 240 550 / 5 240 770 Streptococcus mutans

QUALITY CONTROL
The media, strips and reagents are systematically quality controlled at various stages of their manufacture.
Streamlined quality control may be used to confirm acceptable performance of the API 20 Strep system after shipping-
storage. This methodology may be performed by following the instructions above for testing and meeting the criteria
®
stated in CLSI M50-A Quality Control for Commercial Microbial Identification Systems.
Testing may be conducted using Streptococcus equi spp zooepidemicus ATCC® 700400 to evaluate the performance
of the ARA test. Testing performed by bioMérieux has shown that the ARA test is the most labile on the API 20 Strep
strip. When testing the strip, Stretococcus equi spp zooepidemicus ATCC 700400 can be used to detect degradation.
For those users who are required to perform comprehensive quality control testing with the strip, the following two
strains should be tested to demonstrate positive and negative reactivity for most of the API 20 Strep tests.
1. Streptococcus equi spp zooepidemicus ATCC 700400 2. Streptococcus uberis ATCC 700407
ATCC : American Type Culture Collection, 10801 University Boulevard, Manassas, VA 20110-2209, USA.
VP HIP ESC PYRA αGAL ßGUR ßGAL PAL LAP ADH RIB ARA MAN SOR LAC TRE INU RAF AMD GLYG
1. – – – – – + – + + + + – – + + – – – + +
2. + + + V V + – –* + + + – + + + + + + – –
* This result may vary depending on the culture medium used.
• Inoculum adjusted to between 4.5 and 5.5 McF using DENSIMAT.
• Profiles obtained after : - 4 hours of incubation for tests VP to LAP
- 24 hours of incubation for tests ADH to GLYG.
• Strains cultured on Columbia sheep blood agar.
It is the responsibility of the user to perform Quality Control in accordance with any local applicable regulations.

LIMITATIONS OF THE METHOD • After 24 hours of incubation:

• The API 20 Strep system is intended uniquely for the 3782 collection strains and strains of various origins
identification of those species included in the database belonging to species included in the database were
(see Identification Table at the end of this package tested :
insert). It cannot be used to identify any other - 93.4 % of the strains were correctly identified (with or
microorganisms or to exclude their presence. without supplementary tests).
• Certain strains of Streptococcus porcinus may be - 3.2 % of the strains were not identified.
identified as Streptococcus agalactiae. - 3.4 % of the strains were misidentified.
• Only pure cultures of a single organism should be used.
WASTE DISPOSAL
RANGE OF EXPECTED RESULTS Dispose of used or unused reagents as well as any other
Consult the Identification Table at the end of this package contaminated disposable materials following procedures
insert for the range of expected results for the various for infectious or potentially infectious products.
biochemical reactions. It is the responsibility of each laboratory to handle waste
and effluents produced according to their type and degree
PERFORMANCE of hazardousness and to treat and dispose of them (or
• After 4 hours of incubation: have them treated and disposed of) in accordance with
2336 collection strains and strains of various origins any applicable regulations.
belonging to species included in the database were
tested : WARRANTY
- 87.9 % of the strains were correctly identified (with or bioMérieux disclaims all warranties, express or implied,
without supplementary tests). including any implied warranties of MERCHANTABILITY
- 5.7 % of the strains were not identified. AND FITNESS FOR A PARTICULAR USE. bioMérieux
- 6.4 % of the strains were misidentified. shall not be liable for any incidental or consequential
damages. IN NO EVENT SHALL BIOMERIEUX’S
LIABLITY TO CUSTOMER UNDER ANY CLAIM
EXCEED A REFUND OF THE AMOUNT PAID TO
BIOMERIEUX FOR THE PRODUCT OR SERVICE
WHICH IS THE SUBJECT OF THE CLAIM.

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READING TABLE
QTY RESULTS
TESTS ACTIVE INGREDIENTS REACTIONS/ENZYMES
(mg/cup.) NEGATIVE POSITIVE
VP 1 + VP 2 / wait 10 min (3)
acetoin production
VP sodium pyruvate 1.9 Colorless Pink-Red
(Voges Proskauer)
NIN / wait 10 min
HIP hippuric acid 0.4 hydrolysis (HIPpuric acid) Colorless/Pale blue
Dark blue/Violet
Bluish-grey
4 hrs. 24 hrs. 4 hrs. 24 hrs.
Colorless
esculin 1.16 ß-glucosidase hydrolysis Colorless Black
ESC Pale yellow Black
ferric citrate 0.152 (ESCulin) Pale yellow Grey
Light grey
ZYM A + ZYM B / 10 min (PYRA to LAP) (1)
if necessary, decolorize with intense light
pyroglutamic acid- Colorless or
PYRA 0.0256 PYRrolidonyl Arylamidase Orange
ß-naphthylamide very pale orange
6-bromo-2-naphthyl-
αGAL 0.0376 α-GALactosidase Colorless Violet
αD-galactopyranoside
naphthol ASBI-
ßGUR 0.0537 ß-GlUcuRonidase Colorless Blue
glucuronic acid
2-naphthyl- Colorless or
ßGAL 0.0306 ß-GALactosidase Violet
ßD-galactopyranoside Very pale violet
Colorless or
PAL 2-naphthyl phosphate 0.0244 ALkaline Phosphatase Violet
Very pale violet
LAP L-leucine-ß-naphthylamide 0.0256 Leucine AminoPeptidase Colorless Orange
ADH L-arginine 1.9 Arginine DiHydrolase Yellow Red
4 hrs. 24 hrs. 4 hrs. 24 hrs.
Orange/ Orange/
RIB D-ribose 1.4 acidification (RIBose) Red Yellow
Red Yellow
Orange/ Orange/
ARA L-arabinose 1.4 acidification (ARAbinose) Red Yellow
Red Yellow
Orange/ Orange/
MAN D-mannitol 1.36 acidification (MANnitol) Red Yellow
Red Yellow
Orange/ Orange/
SOR D-sorbitol 1.36 acidification (SORbitol) Red Yellow
Red Yellow
D-lactose Orange/ Orange/
LAC 1.4 acidification (LACtose) Red Yellow
(bovine origin) Red Yellow
Orange/ Orange/
TRE D-trehalose 1.32 acidification (TREhalose) Red Yellow
Red Yellow
Orange/ Orange/
INU inulin 5.12 acidification (INUlin) Red Yellow
Red Yellow
Orange/ Orange/
RAF D-raffinose 3.12 acidification (RAFfinose) Red Yellow
Red Yellow
Orange/ Orange/
AMD starch (2) 2.56 acidification (AmiDon) Red Yellow
Red Yellow
GLYG glycogen 1.28 acidification (GLYcoGen) Red or Orange Bright yellow
(1) During a second reading after 24 hours of incubation, a deposit may be noticed in the tubes where the ZYM A and ZYM B reagents
have been added. This phenomenon is normal and should not be taken into consideration.
(2) The acidification of starch is frequently weaker than that of other sugars.
(3) A pale pink color obtained after 10 minutes should be considered negative.
• The quantities indicated may be adjusted depending on the titer of the raw materials used.
• Certain cupules contain products of animal origin, notably peptones.

PROCEDURE p. I LITERATURE REFERENCES p. III

IDENTIFICATION TABLE p. II INDEX OF SYMBOLS p. IV

BIOMERIEUX, the blue logo, API and apiweb are used, pending and/or registered trademarks belonging to bioMérieux SA or one of its subsidiaries.
CLSI is a trademark belonging to Clinical Laboratory and Standards Institute, Inc.
ATCC is a trademark belonging to American Type Culture Collection.
Any other name or trademark is the property of its respective owner.

bioMérieux SA bioMérieux, Inc

RCS LYON 673 620 399 Box 15969,
69280 Marcy-l'Etoile / France Durham, NC 27704-0969 / USA
Tél. 33 (0)4 78 87 20 00 Tél. (1) 919 620 20 00
Fax 33 (0)4 78 87 20 90 Fax (1) 919 620 22 11
www.biomerieux.com Imprimé en France
api® 20 Strep 07625L - xl - 2010/07

METHODOLOGIE / PROCEDURE / METHODIK / TECNICA / PROCEDIMENTO /

ΔΙΑΔΙΚΑΣΙΑ / METOD / METODE / METODYKA

- Cocci / Kokken / Cocos / Cocchi /

Κόκκοι / Kocker / Kokker / Ziarniaki
- Gram +
- Catalase / Katalase / Catalasa /
Gélose au sang / Blood agar / Blutagar /
Catalasi / Καταλάση / Katalas /
Agar con sangre / Agar al sangue / Gelose de sangue /
Αιματούχο άγαρ / Blodagar / Agar krwawy Katalase / Katalaza –

Gélose Columbia au sang / Columbia blood agar /

Columbia Blutagar / Agar Columbia con sangre /
Agar Columbia al sangue / Gelose Columbia de sangue /
Αιματούχο άγαρ Columbia / Columbia blodagar /
Agar Columbia z krwią

24:00 ± 2:00 02 36°C ± 2°C

> 4 McF

API Suspension Medium 2 ml

~ 500 µl VP ADH

ADH

API GP Medium

RIB GLYG

RIB GLYG

4:00 – 4:30 36°C ± 2°C

24:00 36°C VP : VP 1 + VP 2
± 2:00 ± 2°C HIP : NIN
API 20 Strep PYRA LAP : ZYM A + ZYM B

+ - + - + -


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TABLEAU D'IDENTIFICATION / IDENTIFICATION TABLE / PROZENTTABELLE / TABLA DE IDENTIFICACION /

TABELLA DI IDENTIFICAZIONE / QUADRO DE IDENTIFICAÇÃO / ΠΙΝΑΚΑΣ ΤΑΥΤΟΠΟΙΗΣΗΣ /
IDENTIFIERINGSTABELL / IDENTIFIKATIONSTABEL / TABELA IDENTYFIKACYJNA
% de réactions positives après 4/24 h à 36°C ± 2°C / % of reactions positive after 4/24 hrs. at 36°C ± 2°C /
% der positiven Reaktionen nach 4/24 h bei 36°C ± 2°C / % de las reacciones positivas después de 4/24 H a 36°C ± 2°C /
% di reazioni positive dopo 4/24 ore a 36°C ± 2°C / % das reacções positivas após 4/24 H a 36°C ± 2°C /
% θετικών αντιδράσεων μετά από 4/24 ώρες στους 36°C ± 2°C / % positiva reaktioner efter 4/24 timmar vid 36°C ± 2°C /
% positive reaktioner efter 4/24 timer ved 36°C ± 2°C/ % pozytywnych reakcji po 4/24 godzinach w 36°C ± 2°C
API 20 STREP V7.0 VP HIP ESC PYRA AGAL BGUR BGAL PAL LAP ADH RIB ARA MAN SOR LAC TRE INU RAF AMD GLYG HEM
Abiotrophia defectiva 25 0 15 99 100 0 100 0 92 0 0 0 0 0 98 100 5 92 99 0 0
Aerococcus urinae 3 99 24 12 0 52 41 50 92 28 28 0 32 13 56 64 1 1 40 0 0
Aerococcus viridans 1 13 50 96 54 33 16 37 1 5 1 83 33 85 70 83 99 33 41 70 33 1
Aerococcus viridans 2 15 70 50 76 10 20 25 1 5 5 25 1 35 2 70 89 1 5 24 1 5
Aerococcus viridans 3 22 88 99 40 85 48 14 14 1 1 8 2 82 5 91 99 37 99 14 1 1
Alloiococcus otitis 0 25 0 100 0 3 100 1 90 0 0 0 0 0 0 20 0 0 0 0 0
Enterococcus avium 99 60 99 94 15 0 24 1 99 0 99 40 100 95 95 99 1 40 15 0 1
Enterococcus durans 100 43 100 97 32 2 76 1 91 100 99 15 2 0 84 76 0 0 56 0 18
Enterococcus faecalis 99 46 99 97 1 0 21 4 99 92 98 1 98 92 92 100 0 1 96 2 1
Enterococcus faecium * 94 43 99 95 42 1 89 1 97 93 85 70 78 18 84 98 15 10 60 3 1
Gardnerella vaginalis 0 95 0 1 0 1 53 0 99 0 46 6 1 0 1 0 0 0 73 53 0
Gemella haemolysans 25 0 0 70 0 0 1 84 40 1 1 0 20 10 5 2 0 0 10 5 1
Gemella morbillorum 3 0 0 35 0 0 10 35 86 4 5 0 1 0 1 11 3 1 16 5 0
Globicatella sanguinis 4 40 98 40 52 16 100 0 9 0 76 95 71 47 76 100 71 95 100 90 0
Granulicatella adiacens 0 0 10 80 0 25 0 0 99 0 0 0 0 0 0 0 0 0 0 0 0
Lactococcus lactis ssp cremoris 98 25 41 1 23 0 18 4 88 0 27 0 17 0 97 30 0 15 25 0 0
Lactococcus lactis ssp lactis 90 40 99 35 3 0 35 3 96 95 95 15 45 1 72 87 4 5 90 3 1
Leuconostoc spp 91 1 60 5 55 0 65 2 70 10 37 35 29 4 35 65 0 42 11 0 0
Listeria spp 97 79 98 0 0 0 0 0 85 0 6 0 0 0 49 92 1 1 72 0 26
Streptococcus agalactiae ** 100 99 1 1 4 79 1 96 99 99 98 0 1 1 50 87 0 1 35 4 75
Streptococcus anginosus 100 0 100 0 44 0 1 99 100 100 0 0 33 0 99 88 0 44 97 0 37
Streptococcus bovis I 99 1 100 1 34 2 1 0 100 0 0 1 97 1 100 100 65 98 98 98 1
Streptococcus bovis II 1 100 0 1 0 58 0 0 0 100 0 0 0 0 0 90 0 0 97 97 97 0
Streptococcus bovis II 2 100 2 100 0 89 97 99 0 100 0 0 0 0 0 100 100 0 72 31 5 0
Streptococcus bovis II 3 99 1 100 0 99 0 6 0 100 0 0 0 0 0 100 6 6 100 93 0 0
Streptococcus bovis II 4 98 1 100 0 97 2 10 0 100 1 1 32 1 1 98 40 84 99 99 97 0
Streptococcus canis 0 1 25 4 95 1 80 100 100 100 100 0 0 0 99 1 0 1 99 0 100
Streptococcus constellatus 100 1 27 0 0 0 5 99 100 100 0 0 0 0 10 72 0 0 12 0 61
Streptococcus dys.ssp dysgalactiae 0 0 1 1 1 99 0 100 99 100 99 0 1 50 86 100 0 1 99 30 2
Streptococcus dys.ssp equisimilis 0 1 25 1 1 99 1 99 100 97 97 1 1 1 45 99 0 1 98 40 94
Streptococcus equi ssp equi 1 0 1 0 0 100 0 100 100 100 0 0 0 0 0 1 0 0 100 100 100
Streptococcus equi ssp zooepidemicus 0 1 15 0 0 100 1 99 100 99 85 0 0 99 100 0 0 0 99 99 99
Streptococcus equinus 100 0 95 0 28 0 1 1 100 0 0 0 30 0 25 7 25 15 17 10 0
Streptococcus group L 1 75 1 0 0 100 1 100 100 100 100 0 0 0 75 100 0 0 100 98 94
Streptococcus intermedius 100 0 87 0 0 0 44 99 100 100 0 0 0 0 99 99 3 3 99 0 40
Streptococcus mitis 1 1 0 3 1 21 0 25 35 99 19 14 1 0 1 94 7 3 26 67 5 0
Streptococcus mitis 2 0 0 3 0 31 0 35 50 100 99 1 0 1 0 100 1 1 31 84 0 0
Streptococcus mutans 99 0 99 1 64 0 1 1 100 18 0 0 99 90 90 100 81 81 1 0 1
Streptococcus oralis 0 0 1 1 50 0 46 72 100 5 1 0 1 0 99 32 1 72 96 0 0
Streptococcus pneumoniae 0 0 39 60 70 3 79 3 100 57 3 1 0 0 99 98 64 87 84 10 1
Streptococcus porcinus 100 5 99 1 19 99 1 97 97 100 98 0 88 88 83 99 0 0 50 0 100
Streptococcus pyogenes 0 1 5 98 0 15 0 100 100 99 0 0 8 1 99 98 0 1 61 22 98
Streptococcus salivarius 85 0 98 1 8 0 70 20 100 0 0 0 5 1 86 67 34 88 74 1 1
Streptococcus sanguinis 0 1 42 0 63 0 1 5 100 90 0 0 1 48 83 98 33 55 67 0 0
Streptococcus suis I 0 1 82 53 80 94 76 1 100 91 0 0 7 0 94 100 75 0 100 89 0
Streptococcus suis II 0 1 70 41 91 91 52 3 100 95 0 0 3 1 99 98 63 93 99 96 2
Streptococcus uberis 99 98 100 35 10 86 5 30 100 98 99 0 99 98 99 99 87 10 50 20 0
Enterococcus casseliflavus
* si / if / wenn / se / εάν / om / hvis / gdy :
VancoR / VanR / VAN = R : {
ou / or / od. / o / ή / eller / lub
Enterococcus gallinarum }possible / möglich / posible / possibile / possível / πιθανόν /
möjlig / mulig / możliwość.
** Voir § Limites du test / See § Limitations of the method / Siehe § Limitierungen / Ver § Límites del método / Vedere § Limiti del metodo /
Consultar § Limites do teste / Bλέπε § Περιορισμοί Μεθόδου / Se avsnittet “Metodens begränsningar“ / Se § Metodens begrænsninger /
Patrz § Ograniczenia testu

bioMérieux SA II
api® 20 Strep 07625L - xl - 2010/07

BIBLIOGRAPHIE / LITERATURE REFERENCES / LITERATUR /

BIBLIOGRAFIA / ΑΝΑΦΟΡΕΣ ΑΡΘΡΟΓΡΑΦΙΩΝ / REFERENSLITTERATUR /
LITTERATURHENVISNINGER / PIŚMIENNICTWO
1. APPELBAUM P.C., CHAURUSHIYA P.S., JACOBS M.R., 6. HUMAN R.P. and TILLOTSON G.S.
DUFFETT A. Identification of Gardnerella vaginalis with the API 20 Strep
Evaluation of the Rapid Strep System for Species System.
Identification of Streptococci. (1985) J. Clin. Microbiol., 21, 985-986.
(1984) J. Clin. Microbiol., 19, 588-591. 7. KLOOSTERMAN R.E., CULLEN K.D., McCLATCHEY K.D.
2. BALL L.C., COLMAN G. Comparison of Two Commercial Systems for the Rapid
A Comparison of Conventional Methods and API Galleries for Identification of Streptococci.
the Identification of Streptococci. (1984) ASM ST. LOUIS C198.
(1982) International Meeting on Streptococci and 8. MacGOWAN A.P., MARSHALL R.J., REEVES D.S.
Streptococcal Diseases, LUND SWEDEN, 41-42. Evaluation of API 20 STREP System for Identifying Listeria
3. BANNISTER M.F., BENSON C.E. and SWEENEY C.R. Species.
Rapid Species Identification of Group C Streptococci Isolated (1989) J. Clin. Path., 42, 548-550.
from Horses. 9. RUOFF K.L., KUNZ L.J.
(1985) J. Clin. Microbiol., 21, 524-526. Use of the Rapid STREP System for Identification of Viridans
4. COLMAN G., BALL L.C. Streptococcal Species.
Identification of Streptococci in a Medical Laboratory. (1983) J. Clin. Microbiol., 18, 1138-1140.
(1984) J. Appl. Bact., 57, 1-14. 10. TILLOTSON G.S.
5. FACKLAM R.R., RHODEN D.L., SMITH P.B. An Evaluation of the API 20 Strep System.
Evaluation of the Rapid Strep System for the Identification of (1982) J. Clin. Path., 468-472.
Clinical Isolates of Streptococcus Species. 11. Clinical and Laboratory Standards Institute, M50-A Quality
(1984) J. Clin. Microbiol., 20, 894-898. Control for Commercial Microbial Identification Systems;
Approved Guideline, Vol. 28 n° 23.
.

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api® 20 Strep 07625L - xl - 2010/07

TABLE DES SYMBOLES / INDEX OF SYMBOLS / SYMBOLE / CUADRO DE SIMBOLOS /

TABELLA DEI SIMBOLI / QUADRO DOS SÍMBOLOS / ΠΙΝΑΚΑΣ ΣΥΜΒΟΛΩΝ /
SYMBOLER / SYMBOLFORTEGNELSE / TABELA SYMBOLI
Symbole / Symbol Signification / Meaning / Bedeutung
Símbolo /Simbolo Significado / Significato / Επεξήγηση
Σύμβολο Betydelse / Betydning / Znaczenie
Référence du catalogue
Catalogue number (GB) / Catalog number (US)
Bestellnummer / Número de catálogo / Numero di catalogo
Referência de catálogo / Αριθμός καταλόγου
Katalognummer / Katalognummer / Numer katalogowy
Dispositif médical de diagnostic in vitro
In Vitro Diagnostic Medical Device
In Vitro Diagnostikum
Producto sanitario para diagnóstico in vitro
Dispositivo medico-diagnostico in vitro
Dispositivo médico para diagnóstico in vitro
In Vitro Διαγνωστικό Ιατροτεχνολογικό προϊόν
Medicintekniska produkter för in vitro diagnostik
Medicinsk udstyr til in vitro-diagnostik
Wyrób do diagnostyki In Vitro
Fabricant / Manufacturer / Hersteller / Fabricante
Fabbricante / Κατασκευαστής / Tillverkare / Producent
Limites de température / Temperature limitation
Temperaturbegrenzung / Límite de temperatura
Limiti di temperatura / Limites de temperatura
Περιορισμοί θερμοκρασίας / Temperaturbegränsning
Temperaturbegrænsning / Przestrzegać zakresu temperatury
Utiliser jusque / Use by / Verwendbar bis
Fecha de caducidad / Utilizzare entro / Prazo de validade
Ημερομηνία λήξης / Använd före / Holdbar til / Użyć przed
Code du lot / Batch code
Chargenbezeichnung / Código de lote
Codice del lotto / Código do lote
Αριθμός Παρτίδας / Lot nummer / Lotnummer / Kod partii
Consulter les instructions d'utilisation
Consult Instructions for Use
Gebrauchsanweisung beachten
Consulte las instrucciones de uso
Consultare le istruzioni per l'uso
Consulte as instruções de utilização
Συμβουλευτείτε τις οδηγίες χρήσης
Se handhavandebeskrivningen / Se brugsanvisning
Sprawdź w instrukcji obsługi
Contenu suffisant pour "n" tests
Contains sufficient for <n> tests
Inhalt ausreichend für <n> Prüfungen
Contenido suficiente para <n> ensayos
Contenuto sufficiente per "n" saggi
Conteúdo suficiente para “n” ensaios
Περιεχόμενο επαρκές για «ν» εξετάσεις
Räcker till "n" antal tester
Indeholder tilstrækkeligt til "n" test
Wystarczy na wykonanie <n> testów

bioMérieux SA bioMérieux, Inc

RCS LYON 673 620 399 Box 15969,
69280 Marcy-l'Etoile / France Durham, NC 27704-0969 / USA
Tél. 33 (0)4 78 87 20 00 Tél. (1) 919 620 20 00
Fax 33 (0)4 78 87 20 90 Fax (1) 919 620 22 11
www.biomerieux.com Imprimé en / Printed in France