Gene Expression Real-Time PCR Workflow

Do you have all the pieces of the puzzle?

It’s never been easier to get everything you need for your
real-time PCR experiments.

1
Isolate RNA
Aurum™ Total RNA Kits produce high-quality, DNA-free total RNA
from various starting materials.

2
Synthesize cDNA
iScript™ Reverse Transcription Kits provide simple, fast, and sensitive
first-strand cDNA synthesis.

3
Set Up the Plate
PrimePCR™ Assays — Supermixes — patented* Plastics — patented
gene-specific primers Sso7d fusion polymerase Hard-Shell® PCR Plates
are bench tested for technology provides provide superior stability
proven performance. robust performance. and flatness.

4
Run Real-Time PCR
Bio-Rad’s real-time PCR detection systems feature advanced optical
technology and precise thermal control for optimal performance.

5
Analyze Data
CFX Maestro™ Software provides intuitive setup and data analysis
for all your quantitative PCR needs.

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

Ordering Information
Catalog # Description Catalog # Description
Isolate RNA PCR Plates and Sealer
1 7326820 Aurum Total RNA Mini Kit, 50 preps, includes 43 HSP9601 Hard-Shell Low-Profile 96-Well Skirted PCR Plates,
50 RNA binding columns, 50 capless collection tubes pkg of 50, white shell/clear well, rigid 2-component design
(2.0 ml), 100 capped sample tubes (2.0 ml), 50 capped HSP3805 Hard-Shell 384-Well Skirted PCR Plates, pkg of 50,
sample tubes (1.5 ml), 1 vial lyophilized DNase I, clear shell/white well, for higher fluorescent signals,
RNase-free reagents rigid 2-component design
7326830 Aurum Total RNA Fatty and Fibrous Tissue Kit, 1814000 PX1™ PCR Plate Sealer, includes heat sealing instrument,
50 preps, includes 50 RNA binding columns, 50 capless plate support block that holds 96-well and 384-well plates,
collection tubes (2.0 ml), 100 capped sample tubes (2.0 ml), sealing frame, power cord
50 capped sample tubes (1.5 ml), 1 vial lyophilized DNase I,
RNase-free reagents, plasticware, 50 ml PureZOL™ More at bio-rad.com/web/PlasticSolutions
RNA Isolation Reagent
Run Real-Time PCR
More at bio-rad.com/web/RNASolutions 4 1855195 CFX96 Touch™ Real-Time PCR Detection System,
includes C1000 Touch™ Thermal Cycler Chassis, CFX96™
Synthesize cDNA
2 1708840 iScript Reverse Transcription Supermix for RT-qPCR,
1855485
Optical Reaction Module, USB cable
CFX384 Touch™ Real-Time PCR Detection System,
25 x 20 µl reactions, 4 x 25 µl 5x supermix contains reverse
includes C1000 Touch Thermal Cycler Chassis, CFX384™
transcriptase, RNase inhibitor, dNTPs, primers, MgCl2,
Optical Reaction Module, USB cable
stabilizers; 50 reactions of no-RT control supermix
1725037 iScript Advanced cDNA Synthesis Kit for RT-qPCR, 1855201 CFX Connect™ Real-Time PCR Detection System,
25 x 20 µl reactions, includes 100 µl 5x iScript Advanced includes CFX Connect Thermal Cycler Chassis, CFX Connect
Reaction Mix, 25 µl iScript Advanced Reverse Transcriptase, Optical Reaction Module, USB cable
nuclease-free water
1708890 iScript cDNA Synthesis Kit, 25 x 20 µl reactions, More at bio-rad.com/web/RealTimePCR
includes 100 µl 5x iScript Reaction Mix, 25 µl iScript
Analyze Data
Reverse Transcriptase, nuclease-free water
5 12004110 CFX Maestro Software, Windows PC version. Mac, Security
Edition (U.S. FDA 21 CFR Part 11 compliant), and Russian
More at bio-rad.com/web/cDNASynthesis
and Chinese language versions are also available
Set Up the Plate
3 PrimePCR Assays
More at bio-rad.com/web/CFXMaestro
Bio-Rad offers bench-tested primers and probes in predesigned
plate, custom plate, and individual assay formats. Go to Mac is a trademark of Apple Inc. ROX and SYBR are trademarks of Thermo Fisher
bio-rad.com/web/PrimePCRAssays for more information. Scientific Inc. Bio-Rad Laboratories, Inc. is licensed by Thermo Fisher Scientific
SsoAdvanced™ and iTaq™ Supermixes Inc. to sell reagents containing SYBR Green I for use in real-time PCR, for research
purposes only. Windows is a trademark of Microsoft Corporation.
1725270 SsoAdvanced™ Universal SYBR ® Green Supermix,
200 x 20 µl reactions, 2 ml (2 x 1 ml vials), 2x qPCR mix, Bio-Rad’s thermal cyclers and real-time thermal cyclers are covered by one or more
contains dNTPs, Sso7d fusion polymerase, MgCl2, of the following U.S. patents or their foreign counterparts owned by Eppendorf AG:
SYBR® Green I, ROX Normalization Dyes U.S. Patent Numbers 6,767,512 and 7,074,367.
1725280 SsoAdvanced Universal Probes Supermix, 200 x 20 µl Hard-Shell Plates are covered by one or more of the following U.S. patents or their
reactions, 2 ml (2 x 1 ml vials), 2x qPCR mix, contains foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977;
dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, 6,340,589; and 6,528,302.
ROX Normalization Dyes
1725120 iTaq™ Universal SYBR® Green Supermix, 200 x 20 µl
reactions, 2 ml (2 x 1 ml vials), 2x qPCR mix, contains
dNTPs, iTaq DNA Polymerase, MgCl 2 , SYBR® Green I,
enhancers, stabilizers, fluorescein, and ROX
Normalization Dyes
1725130 iTaq Universal Probes Supermix, 200 x 20 µl reactions,
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, iTaq
DNA Polymerase, MgCl2 , enhancers, stabilizers, ROX
Normalization Dyes

More at bio-rad.com/web/PCRSupermixes

Bio-Rad
Laboratories, Inc.

Life Science Web site bio-rad.com USA 1 800 424 6723 Australia 61 2 9914 2800 Austria 43 01 877 89019 Belgium 32 03 710 53 00 Brazil 55 11 3065 7550
Canada 1 905 364 3435 China 86 21 6169 8500 Czech Republic 36 01 459 6192 Denmark 45 04 452 10 00 Finland 35 08 980 422 00 France 33 01 479 593 00
Group Germany 49 089 3188 4393 Hong Kong 852 2789 3300 Hungary 36 01 459 6190 India 91 124 4029300 Israel 972 03 963 6050 Italy 39 02 49486600
Japan 81 3 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 31 0 318 540 666 New Zealand 64 9 415 2280 Norway 47 0 233 841 30
Poland 36 01 459 6191 Portugal 351 21 4727717 Russia 7 495 721 14 04 Singapore 65 6415 3188 South Africa 36 01 459 6193 Spain 34 091 49 06 580
Sweden 46 08 555 127 00 Switzerland 41 0617 17 9555 Taiwan 886 2 2578 7189 Thailand 66 2 651 8311 United Arab Emirates 971 4 8187300
United Kingdom 44 01923 47 1301

Bulletin 6763 Ver B US/EG 18-0334 0618 Sig 0118