a srt — ee y ESTIMATION of CHOLESTEROL BY CHOD ff, ee Jo AAI ting [oy SH20 [PAP method > Chplataol iw sexu or plasma fd colsstrol it the main Upicl found ba blood, bile oma brabw Ueiute Stu the main Upc auocialed willy oatutoachrotic VOAULOr COE. 9b jk neqpined for the formation dy tunoids andl Coltutan membnanu. The fiver matabotises the cholustenok oud it le transported tw the blood smeam by tipsprotdm. Smorented feels ane found iow IypercholabexoLacnsa hyper ipidazmia. , Aippothy roidizm, wncentnotied diabetu, weplrotic ome, amd canhosis. Decrcaoud Revels ore found Inv! matalsorp- tiew, malnuletiow , Dypertryncidim , anemiat and Var diseases. 4 Ay EEA PRINCIPLE 3 Cholusterol esinase Kydrolyss eakenifpied cholutabls 10 chobubane. The fee choluitol ik oxidised to foun dog proxide, cotatytic, o.ckiow ef proxidoue to fem 0 ted coloured, qproncimine dye Complet. Splenity 9 the colow foumed a diactty propobiewal to the awwourd d}, clrolrterol present im thre sample . | Teacher's Signature © scanned with OKEN Scanner© scanned with OKEN Scanner| Reagent Preparatiow, Reagent ane ready to uce working Reagent » four the coutenta oh 1 bottle o L2 (Emme Reagent 2) into 4 vottde dU (Emme Reagent J). This working raagent ix stable Jor ot Rast Buueke whew sored ab 2-B°C. Upon stinage the Vv working reagent may dusebep o sight punk colour Dower this | | | dou st apes ha performance of the reafert . | \ | | AUematively for fleriibity on much, 4 working rnagent may be made os and whit dubed ty mixing topdtrr 4 pare ob” L( en: Reagent 1) anc 1 port dh L2( Erayme Reagent 2) . Atonabively OB me J, Lt amd O2 mL d L2 may also be usecl jskead, a amb 4 the working raagent dinate Ouning he ons. Teacher's Signature © scanned with OKEN ScannerRuins] ls 2 SoS on (iy BEA) = + - mn Gren { | Tempore arene ee ! we [| wget Pate tem a 1 fiptte_ into clea doy fat tub tabetled ax Blank (8), Standend CS). I | due Test_(T): | CALCULATIONS + amin. Measure omd the absorbance A We Standard (Abs. $) | sample (Abs T) against the Blank, within 60 Min. at [Min well ond inubate at 33'c fer Sari. or ot RT Ne is | \ \ od va Crottnrod iw mq [at 1 Abs. T 20D Abs. S = 0-03 p20 00S = 3x40 = 120mg |e. Teacher's Signature © scanned with OKEN ScannerThis procedure it fimean upto 150 mg [dl ay the value excaade His Lint» ditute the serum will mormal saline ( NaCl 0-37.) amet | repeat the amay. Cotculats the mun uting the prope aitution factor. NOTE © + pnbiccagulants such ox flourider ancl oxalatas result iw false (vo volun. The test is not inptuened by Hb vou uid dom jdt amd pitinubiw upto to mq |dl. inet s\z\ Teacher's Signature © scanned with OKEN Scanner