Centre for Environmental Manage ment of Degra de d Ec osyste ms

University of Delhi QUOTATION INVITATION LETTER Ref: Bar DBT/Equip/07 Date: 06.08.2007

Sealed quotations are invited for the following items so as to reach the undersigned latest by 07.09.2007 Instruction to Suppliers: Two kinds of quotations may be submitted, technical and financial quotations. Technical quotation should consist of all technical details alongwith commercial terms and conditions and delivery schedule. Financial quotation containing item wise price for the items mentioned in the technical quotation and delivery schedule. Both the quotations should be sealed by the supplier in separate covers duly superscribed and both these sealed covers are to be put in a bigger cover which should also be sealed and superscribed. Company should attach users list also. Schedule of requirements: S. No. 1. Item Gel Documentation System No. 01 No.

Specification and allied technical details: (i) High resolution CCD camera Fire wire connectivity 12-bit data acquisition upgradeability (ii) Resolution (iii) Motorized Zoom Lens (iv) Universal Hood 1.4 Mega pixel interline CCD Rapid data transfer for real time imaging. Quantitative, 3.0 orders of dynamic range. Should be upgradeable for chemi application in future 1360x1024 with 4.6x4.6 micron Pixel Size Resolution should be 47.8 m/pixel at max zoom C-mount lens to collect more light to hit CCD, f/1.2;Zoom 8-51mm . Should be light tight with light baffling option. Touch pad controls for iris, zoom, and focus of the lens and for on/off control of epi and

transillumination (v) Large Sample Plate (vi) White Light Epi Illumination (vii) UV Transillumination 302nm UV-B
UV illumination imaging area should be 26 x 25cm.

For imaging of colorimetric blots and colonies. Should be Compatible with a wide range of applications including ethidium bromide, SYBR green, SYBR gold, SYPRO orange, Fluorescein, Radiant Red, Texas Red, SYPRO Red, GelStar, CY3, Rhodamine, Hoechst, Coumarin. Option for easy conversion of system set-up for either fluorescence or colorimetric samples. Threeposition filter slider - two positions for emission filters (fluorescence/colorimetric samples) and one open position for chemiluminescence for future up gradation. Amber Filter with spectral range of 548-630nm for following application:EtBr, SYBR Green/GFP/SYBR Gold/Fluorescein/ CY3/Rhodamine/ SYPRO Ruby/Texas Red, Hoechst/Coumarin. To protect from UV exposure by shutting off UV light source during opening of the main door or pull out drawer. To preserve lamp life, system should automatically shuts off UV and white light lamps after approximately 5 minutes. Latest data processor(BRANDED ONE) with suitable inkjet printer- P4, 512 MB, 80 GB, 17 TFT Flat monitor, CD-DVD Combo Drive, windows XP Pro. Easy user control of UV and White light sources through touch pad. Software should analyze variety of data based on application includingradioactive ,chemiluminescent, fluorescent, color stained sample, Automatic lane

(viii) Filter Slider

(ix) Filters

(x) UV Safety Interlock

(xi) UV and White Lamp Auto Shut-off (xii) Data processor

(xiii) Single Point Control of all Light Sources (xiv) Software with networking

and band detection, Rapid molecular weight detection with multiple regression models, band lane matching analysis, background substraction correction of gradient gels, Accurate concentration analysis using volume tools, recall of lane and sample layouts, molecular weight determination, colony counting, array tools for slot blot, dot blot and other medium density array, Differential display option for study of Gene expression study using mRNA and PCR amplification, Ready agarose 96 plus option,3 D viewer for discriminating two nearby band or spots ,Variable number tandem repeats (VNTR) for microsattalite Study. 2. UV Transilluminator UV Transilluminator should have the following: Should be able to take up Proteins, DNA, and sequencing Gels. Gel visualization area of 25x26 cm Should have UV illumination of 302 nm Should have option for analytical and Preparative switches. 01 No. 01 No.

3. Thermal cycler Specifications for Thermal cycler:

Gradient Thermal Cycler with Peltier heating and cooling based system. Should have block for 96x0.2ml tubes. Should have option for accommodating interchangeable blocks (2X48X0.2 ml wells, 60 wells, 384 wells, etc.) Blocks should have multizone feedback control with four heating or cooling zones. Should have option of block for insitu PCR capable of taking 2x16 glass slides Ramping speed of upto 3 degree C heating per sec. Temperature range of 0-105OC Gradient range of 30-105CC with accuracy of 0.3OC System should be upgradeable to Real Time PCR Alphanumeric LCD display Memory of around 400 programs Adjustable Heated lid and auto start up facility should be there.

It should be a Licensed PCR machine Should come with an APC make online UPS of 2 KVA capacity with battery back up of 60 minutes (via external batteries).

4. Submarine electrophoresis system Specifications for submarine electrophoresis system UV Transparent gel trays with an integrated fluorescent ruler. Multiple options for hand casting gels of different sizes. Combs to fit virtually every need- fixed height Drop-in combs. Easy to replace electrode cassettes. Should have Combs accommodating up to 120 samples Gel trays 15 cm wide IEC 1010 (EN 61010) electrical safety guidelines.

01 No.

Sizes
1. 15X15 cm with UV transparent tray, and 2 nos of 1.5 mm thick combs of 15 & 20 well, comb holder, casting gates and gel caster. - Qty 1 no. 2. 15X20 cm with UV transparent tray and 2 nos of 1.5 mm thick comb of 15 & 20 well with casting gates, gel caster Qty- 1nos. 3. Extra UV transparent tray of size 15X 15 cm & 15 X 20 cm Qty 1 no. each. 4. Extra combs 2 each of 10 well, 15 well & 20 well of 1.5 mm thickness fixed height comb 5. A suitable Power Pac is required to run these electrophoresis, with 10-300V; 4400mA & 75 W (maximum) with constant voltage or constant current; timer 1999min; Pause/resume function; 3 digit LED display; automatic recovery after power failure; safety features like no-load detection, sudden load change detection, overload/short circuit detection, over voltage protection, stackable, Regulatory EN 61010,CE and no. of output jacks- 4 sets in parallel The above quotation should be addressed to Principal Investigator, Development of DNA barcode for Amphibian Fauna of the Western Ghats, Centre for Environmental Management of Degraded Ecosystems, University of Delhi, Delhi 110 007 to be sent on or before 07 April 2007.

Principal Investigator Development of DNA barcode for Amphibian Fauna of the Western Ghats